Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,334,703 A→G 6.9% intergenic (+22/‑206) ispB → / → sfsB octaprenyl diphosphate synthase/malPQ operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,334,7030AG 6.9% 319.9 / 14.3 134intergenic (+22/‑206)ispB/sfsBoctaprenyl diphosphate synthase/malPQ operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (56/61);  new base G (6/3);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-02

Only 100 of 135 total aligned reads displayed.

GGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGA  >  NC_000913/3334557‑3334852
                                                                                                                                                  |                                                                                                                                                     
ggACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcg                                                                                                                                                    >  1:1262308/1‑150 (MQ=255)
ggACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcg                                                                                                                                                    <  2:727890/150‑1 (MQ=255)
ggACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAgcg                                                                                                                                                    >  2:719296/1‑150 (MQ=255)
      gTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCggg                                                                                                                                              <  2:1576346/150‑1 (MQ=255)
       tCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCCCATCCCGCGCGCTGCGCGGGa                                                                                                                                             <  2:996349/150‑1 (MQ=255)
            gTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCGCTGCGCGGGATgagg                                                                                                                                        <  1:182539/150‑4 (MQ=255)
               ccGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATCGCTGTTCACCGCGATCGTTAATCACCTCCCCTCATCACGCGCAGCGCGCGGGATGGTGCCa                                                                                                                                     >  2:42533/1‑150 (MQ=255)
                cGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAg                                                                                                                                    <  1:963045/150‑1 (MQ=255)
                 gAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCACCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGt                                                                                                                                   >  2:678029/1‑150 (MQ=255)
                      agaagCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACACCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGtt                                                                                                                              >  2:331455/1‑150 (MQ=255)
                         agCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCa                                                                                                                           >  2:1389462/1‑150 (MQ=255)
                             gACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATaaa                                                                                                                       <  2:1229795/150‑1 (MQ=255)
                                  aGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTAACCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCAATAAAAacac                                                                                                                  >  1:99731/1‑150 (MQ=255)
                                   gCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACACCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACt                                                                                                                 >  1:215239/1‑150 (MQ=255)
                                     cATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAACCACttt                                                                                                               >  2:890725/1‑149 (MQ=255)
                                      aTCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTAt                                                                                                              <  1:990915/150‑1 (MQ=255)
                                        cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                                                                            >  1:1229188/1‑150 (MQ=255)
                                        cGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTc                                                                                                            >  2:1092494/1‑150 (MQ=255)
                                         gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCCTCGGCCTCGCGCACATCGCTGTTCACCGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGCGATGGTTCCAGTAAGTTCCATATCAACACGTATTcg                                                                                                           >  1:305951/1‑149 (MQ=255)
                                         gCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCa                                                                                                           >  1:472289/1‑150 (MQ=255)
                                          cAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGGTCCAGTAAGTTCCATAAAACCACCTATTGAg                                                                                                          >  2:1066965/1‑150 (MQ=255)
                                           aGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGCTCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGGTCCATAAAAACACTGATTCCGc                                                                                                         >  1:68978/1‑150 (MQ=255)
                                            gCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTGCAGTAAGTTCCATAAAAACACATATTCAGct                                                                                                        >  2:536585/1‑150 (MQ=255)
                                                    ggTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGCTGGTTCCAGTAAGTTCCATAAATCCCCTGATGCAGCGCtatcccc                                                                                                >  2:318291/1‑146 (MQ=255)
                                                     gTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCtata                                                                                                   >  1:998619/1‑146 (MQ=255)
                                                      tGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACAcc                                                                                              <  2:1541129/150‑1 (MQ=255)
                                                       gCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCTATAAAAACACTTATTCAGCTCTATAACACCt                                                                                             <  1:2499/150‑1 (MQ=255)
                                                          cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaa                                                                                          >  1:1194342/1‑150 (MQ=255)
                                                          cccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaa                                                                                          >  2:278797/1‑150 (MQ=255)
                                                           ccGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGaaa                                                                                         <  2:1229188/150‑1 (MQ=255)
                                                            cGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTGAGCTCTATAACACCGGaaaa                                                                                        >  1:597521/1‑150 (MQ=255)
                                                               acacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                     <  2:907387/150‑1 (MQ=255)
                                                               acacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATa                                                                                     <  1:557599/150‑1 (MQ=255)
                                                                cacCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAg                                                                                    <  1:1710186/150‑1 (MQ=255)
                                                                 cccccTTGGCGAGAATCACTCATCGGCCTCGCTCACATCGCTGGTCAAGGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg                                                                                   <  2:130785/149‑1 (MQ=255)
                                                                 acCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg                                                                                   <  1:1017857/150‑1 (MQ=255)
                                                                 acCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAgg                                                                                   <  1:1464009/150‑1 (MQ=255)
                                                                  ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAggg                                                                                  <  2:1406962/150‑1 (MQ=255)
                                                                  ccccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCCCGCGGGCGGGGTGGTTGCCGTAAGTTGCAAAAAAACCAATATTAAGCTCTATAACACCGGTAAAATAtgg                                                                                  >  2:1323047/1‑150 (MQ=255)
                                                                   cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATGCCGCCCAGAGCGCGGGAGGGGTCCAGGAAGTTCCCTAAAAACACTTATTCAGCTCAATAACAACTGAAAAATAGGGa                                                                                 >  2:850786/1‑150 (MQ=255)
                                                                   cccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGa                                                                                 <  2:1262308/150‑1 (MQ=255)
                                                                    ccTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTGCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAt                                                                                >  1:1402286/1‑150 (MQ=255)
                                                                     cTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATGCCCCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACCCGTATTAAGCTCGTTAACACCAGAAAAATAGGGAtg                                                                               >  1:1268160/1‑150 (MQ=255)
                                                                        ggCGAGAAGCACTCATCGGCCGCGCGCACATCGCTGTTCAACGCGTTCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGTATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGCCATGTGc                                                                            <  2:201380/150‑1 (MQ=255)
                                                                          cGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTTATTTGCATAAAAAAAAATAATAAGCTATATAACAACTGAAAAAAATGGTAGTGCAt                                                                          >  2:1694555/1‑150 (MQ=255)
                                                                           gagaAGCACTCATCGGCCTCGCGCACATCGCGGGTCGACGCGATCGTTAAGCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATc                                                                         <  2:1093377/150‑1 (MQ=255)
                                                                            agaAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCt                                                                        >  1:1497209/1‑150 (MQ=255)
                                                                             gtcgCACTCAGCGGCCTCGCGCACATCGCTGATCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGGGCATCtt                                                                       <  2:257179/147‑1 (MQ=255)
                                                                               aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta                                                                     >  2:983170/1‑150 (MQ=255)
                                                                               aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta                                                                     <  1:1018423/150‑1 (MQ=255)
                                                                               aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTta                                                                     <  1:801747/150‑1 (MQ=255)
                                                                               aGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCACGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACAATTATTCAGATCGATAACACCTGAAAAAAAGTGATGTGAATCTTta                                                                     >  1:836557/1‑150 (MQ=255)
                                                                                  aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTtatat                                                                  <  2:1118112/150‑1 (MQ=255)
                                                                                  aCTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGGTCCATAAAAACACTTATTCAGCTCTATAACCCCGGAAAAATAGGGATTTGCATATTtatat                                                                  >  1:451835/1‑150 (MQ=255)
                                                                                    tCATCGGCCTCGAGCACATCGCTGTTCAACGAGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTatatat                                                                <  1:331455/150‑1 (MQ=255)
                                                                                     cATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTCCAGGAAGTTCCATAAAAACACTTATTCCGCTCTATTACCCCGGAAAAATAGGGATGTGGATCTTTTTATAtt                                                               >  1:1099165/1‑150 (MQ=255)
                                                                                       tCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTTAGTTCCAGAAAAACACTTATTCAGCTCTATAACACCGGAAAAATAGGGATGTGCCTCTTTATATATTCt                                                             >  1:136516/1‑150 (MQ=255)
                                                                                            cTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGGATCTTTATATAATCTGAata                                                        >  2:141492/1‑150 (MQ=255)
                                                                                              cgcgCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGTTACAGTAAGTTACATAAAAAAACATATTAAGCTCTATAACACCAGAAAAATATGGATGTGCATCTTTATATATTATGAATAtt                                                      >  2:1410704/1‑150 (MQ=255)
                                                                                                cgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGGTGGTTCCAGTAAGTTGCATAAAAACACTTATTGAGGTCTTTAACACCTGAAAAATAGGGATGTGGATCTTTATTTATTATGAAttttga                                                    >  1:573585/1‑148 (MQ=255)
                                                                                                cgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTca                                                    <  2:1662240/150‑1 (MQ=255)
                                                                                                cgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTca                                                    >  1:843046/1‑150 (MQ=255)
                                                                                                cgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCCGGGGATGGTTCCAGTAAGTAACATAAAAACAATTATTCAGCAATATAACACCTCAAAAATAGGGATGTGCATATTTATATATTATGAATAttaa                                                    >  1:627832/1‑148 (MQ=255)
                                                                                                cgcACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCCCGCCGCGCGCGGGATGGTGCCCGGAAGTTCCCTTAAACCCCTTATTGAGCTCAATAACACCAGAAAAATAGGGATGGTGATCTGTATATATTCTGTATATTca                                                    >  2:615216/1‑150 (MQ=255)
                                                                                                  cacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGa                                                           <  2:902184/141‑1 (MQ=255)
                                                                                                  cacaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTcaca                                                  <  1:590482/150‑1 (MQ=255)
                                                                                                   acaTCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCacac                                                 <  1:1694555/150‑1 (MQ=255)
                                                                                                   acaTCGCTGTTCAACGCGATCGTTAATCCACTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCacac                                                 >  1:1215680/1‑150 (MQ=255)
                                                                                                      tCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACActct                                              >  1:241271/1‑150 (MQ=255)
                                                                                                         cTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTa                                           <  1:719296/150‑1 (MQ=255)
                                                                                                           gTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACa                                         <  2:234085/150‑1 (MQ=255)
                                                                                                            ttCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCCCAGCGCGCGGGATGGTGCCAGTAAGTTCCATATAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTAAAATATTCTGAAAATTCACACTCTTTACAg                                        >  2:925598/1‑150 (MQ=255)
                                                                                                             tCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCAAGTAAGTTCCATTAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGAGGTGCATCTTTATATATTCTGAATATTCACACTCTTGACAgg                                       >  1:1167504/1‑150 (MQ=255)
                                                                                                                actcgATCGTTAATCCCCTCCCATCATACATCGCATCGAGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTAATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAAc                                    <  1:102756/147‑1 (MQ=255)
                                                                                                                 cgcgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACt                                   <  2:843046/150‑1 (MQ=255)
                                                                                                                  gcgATCGTTAATCCCCTCCCCTCATCCCGCGCCGCGCGCGGGATGGGTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtt                                  >  1:548514/1‑150 (MQ=255)
                                                                                                                   cgATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTAAGCTCTATAACACCAGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttt                                 >  1:1593526/1‑150 (MQ=255)
                                                                                                                    gATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt                                <  1:4560/150‑1 (MQ=255)
                                                                                                                    gATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACtttt                                <  1:1515129/150‑1 (MQ=255)
                                                                                                                     aTCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACttttt                               <  1:1389462/150‑1 (MQ=255)
                                                                                                                       cGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTag                             <  2:891991/150‑1 (MQ=255)
                                                                                                                        gTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTaga                            <  1:278797/150‑1 (MQ=255)
                                                                                                                          tAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACCCGTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGCACTTTTTagagg                          >  2:670569/1‑149 (MQ=255)
                                                                                                                           aaTCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCa                         <  1:708121/150‑1 (MQ=255)
                                                                                                                                cctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACACTTTACAGGAACTTTTTAGAGCAATAgg                    <  2:817707/150‑1 (MQ=255)
                                                                                                                                cctcccctcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATATTTATATATTCTGAATATTAACACTCTTTACAGTAACTTTTTAGAGCAATAgg                    >  2:677397/1‑150 (MQ=255)
                                                                                                                                      accATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCa              >  1:599088/3‑150 (MQ=255)
                                                                                                                                       tcATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAg             >  2:954821/1‑150 (MQ=255)
                                                                                                                                        cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAgg            >  2:657591/1‑150 (MQ=255)
                                                                                                                                        cATCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCATTACAGGAACTTTTTAGAGCAATAGGCCATCAgg            >  2:651616/1‑150 (MQ=255)
                                                                                                                                         aTCCCGCGCGCTGTGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa           >  2:1388201/1‑150 (MQ=255)
                                                                                                                                         aTCCCGCGCGCTGCGCGGGATGGTTCCAGTAAGTTCCATAAAAAAACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATAAAATCTGAATATTGACACTCTTTACAGGAACTTTTTAGAGCTATAGGCCATCAGGa           >  1:49500/1‑150 (MQ=255)
                                                                                                                                         aTCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGa           >  2:1697641/1‑150 (MQ=255)
                                                                                                                                          tCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGATCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAg          <  1:920135/150‑1 (MQ=255)
                                                                                                                                           cccGCGCAGCGCGCGGGATGGTTCCAGTAATTTCCATAAATACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt         <  1:1128470/150‑1 (MQ=255)
                                                                                                                                           cccGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt         <  1:1092494/150‑1 (MQ=255)
                                                                                                                                           cccGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGt         <  2:136785/150‑1 (MQ=255)
                                                                                                                                            ccGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGta        >  1:633624/1‑150 (MQ=255)
                                                                                                                                            ccGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGta        >  2:711311/1‑150 (MQ=255)
                                                                                                                                                  aGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGa  <  2:550016/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGACGCGTCAGCGTGCCGAGGAAGAAGCAGACAAAGCCATCGCAGCGTTACAGGTGCTCCCGGACACCCCTTGGCGAGAAGCACTCATCGGCCTCGCGCACATCGCTGTTCAACGCGATCGTTAATCCCCTCCCCTCATCCCGCGCAGCGCGCGGGATGGTTCCAGTAAGTTCCATAAAAACACTTATTCAGCTCTATAACACCTGAAAAATAGGGATGTGCATCTTTATATATTCTGAATATTCACACTCTTTACAGGAACTTTTTAGAGCAATAGGCCATCAGGAGTATAGTGA  >  NC_000913/3334557‑3334852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: