Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,349,163 A→G 6.9% V216A (GTG→GCG) ‡ mtgA ← biosynthetic peptidoglycan transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,349,1630AG 6.9% 365.7 / 13.1 131V216A (GTG→GCG) ‡mtgAbiosynthetic peptidoglycan transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (62/58);  new base G (6/3);  total (69/62)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 131 total aligned reads displayed.

TATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAA  >  NC_000913/3349015‑3349311
                                                                                                                                                    |                                                                                                                                                    
tATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCCGCTGGTGTTGTTGCATAAACGGCGCACCGCCTCACTGATACCTCTGCCGTAAAATCCCCGCCGGCCGGCTACGCAc                                                                                                                                                     >  1:1033452/1‑150 (MQ=255)
tATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCAc                                                                                                                                                     >  1:683176/1‑150 (MQ=255)
tATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCAc                                                                                                                                                     >  1:547098/1‑150 (MQ=255)
 aTTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCAAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACg                                                                                                                                                    >  1:1646484/1‑150 (MQ=255)
   tGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTa                                                                                                                                                  >  2:1315460/1‑150 (MQ=255)
    gCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAg                                                                                                                                                 <  2:1548828/150‑1 (MQ=255)
    gCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCGAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAg                                                                                                                                                 >  2:1416911/1‑150 (MQ=255)
      ccTGCAGAAAGCTCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTCCGCACGTAGcc                                                                                                                                               >  1:457507/1‑150 (MQ=255)
      ccTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGcc                                                                                                                                               <  2:1298294/150‑1 (MQ=255)
      ccTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGcc                                                                                                                                               <  1:1142262/150‑1 (MQ=255)
       cTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGccc                                                                                                                                              <  2:1373340/150‑1 (MQ=255)
         gCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtc                                                                                                                                            <  2:1559059/150‑4 (MQ=255)
         gCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGa                                                                                                                                            >  1:1027990/1‑150 (MQ=255)
          cAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACCTAGCCCGAt                                                                                                                                           >  2:727131/1‑150 (MQ=255)
              aaGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtcag                                                                                                                                          <  1:280754/147‑6 (MQ=255)
              aaGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtcag                                                                                                                                          <  1:791701/147‑6 (MQ=255)
              aaGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtcag                                                                                                                                          >  2:791701/1‑142 (MQ=255)
              aaGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGTGCGTAGccgtcag                                                                                                                                          >  2:280754/1‑142 (MQ=255)
                  tCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgagga                                                                                                                                   <  2:367706/150‑1 (MQ=255)
                   cTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCgaggag                                                                                                                                  <  2:178823/150‑1 (MQ=255)
                      cAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACt                                                                                                                               >  1:497398/1‑150 (MQ=255)
                          cTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGa                                                                                                                           <  1:285522/150‑1 (MQ=255)
                          cTCGTGAGAGCAAAACGCTGGCACTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGa                                                                                                                           <  1:1223430/150‑1 (MQ=255)
                            cGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGaaa                                                                                                                         <  1:1233461/150‑1 (MQ=255)
                            cGTGAGAGCAAAACGCTGGCCCTCACATCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGaaa                                                                                                                         <  1:345130/150‑1 (MQ=255)
                             gTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAAc                                                                                                                        <  2:181731/150‑1 (MQ=255)
                              tGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACg                                                                                                                       >  1:1582401/1‑150 (MQ=255)
                                   gCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTg                                                                                                                  >  1:467283/1‑150 (MQ=255)
                                    cAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTgg                                                                                                                 >  2:434792/1‑150 (MQ=255)
                                    cAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACAACTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGACCGATGGCGAGGAGAATTTGAAACGAAGtct                                                                                                                 >  2:1565182/1‑148 (MQ=255)
                                        aCGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTa                                                                                                             <  1:291540/150‑1 (MQ=255)
                                          gCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAgg                                                                                                           <  2:1494512/150‑1 (MQ=255)
                                           cgggCCCTCACTTCGCGCGAAGCTTAATCCAGCTCGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGAAAAATCCACTCCTGACGTCTAATAACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGt                                                                                                          <  2:253069/148‑1 (MQ=255)
                                           cTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGt                                                                                                          >  1:182255/1‑150 (MQ=255)
                                              gCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAAt                                                                                                       <  2:1294022/150‑1 (MQ=255)
                                              gCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAAt                                                                                                       >  2:1174433/1‑150 (MQ=255)
                                                  tCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCTCGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAg                                                                                                   <  1:1618146/150‑1 (MQ=255)
                                                   cACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGc                                                                                                  <  2:1363281/150‑1 (MQ=255)
                                                     cTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTg                                                                                                <  1:1579122/150‑1 (MQ=255)
                                                     cTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTg                                                                                                >  1:729823/1‑150 (MQ=255)
                                                      ttCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGc                                                                                               <  1:1163680/150‑1 (MQ=255)
                                                       tCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGcc                                                                                              <  2:509435/150‑1 (MQ=255)
                                                        cgcgcgAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTa                                                                                                         >  1:1702710/1‑138 (MQ=255)
                                                        cgcgcgAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTa                                                                                                         <  2:1702710/138‑1 (MQ=255)
                                                              aaGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATg                                                                                       <  1:1692482/150‑1 (MQ=255)
                                                                 cTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAg                                                                                    >  2:1208114/1‑150 (MQ=255)
                                                                   tAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCt                                                                                  >  2:1503374/1‑150 (MQ=255)
                                                                   tAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCt                                                                                  <  2:1027990/150‑1 (MQ=255)
                                                                      tCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTcc                                                                               <  2:467283/150‑1 (MQ=255)
                                                                      tCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTcc                                                                               <  2:1256589/150‑1 (MQ=255)
                                                                           cTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCa                                                                                             <  1:877954/131‑1 (MQ=255)
                                                                           cTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCa                                                                                             >  2:877954/1‑131 (MQ=255)
                                                                           cTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACAACTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCg                                                                          >  1:459409/1‑150 (MQ=255)
                                                                              gtgtTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGAcggtgt                                                                       >  2:932936/1‑145 (MQ=255)
                                                                                 ttgttgCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCGGCTTCCGACCGGGTAAg                                                                    >  1:1667976/1‑150 (MQ=255)
                                                                                  tgttgCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGt                                                                   >  2:969050/1‑150 (MQ=255)
                                                                                  tgttgCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGt                                                                   >  2:354228/1‑150 (MQ=255)
                                                                                     tgCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTg                                                                >  1:650822/1‑150 (MQ=255)
                                                                                     tgCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTg                                                                <  1:1208114/150‑1 (MQ=255)
                                                                                       cATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCt                                                              <  1:1194537/150‑1 (MQ=255)
                                                                                       cATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCt                                                              <  1:384134/150‑1 (MQ=255)
                                                                                             cGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCggg                                                        <  1:453810/150‑1 (MQ=255)
                                                                                                cTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGttt                                                     <  1:1326037/150‑1 (MQ=255)
                                                                                                cTCACCGACTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATCCAGATGCCAGTAATGCAGCTTACTACCGGGTAAGTTAGATCGATGGttt                                                     >  2:914742/1‑150 (MQ=255)
                                                                                                  cACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTtgt                                                   >  1:502278/1‑150 (MQ=255)
                                                                                                  cACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTtgt                                                   <  1:1676752/150‑1 (MQ=255)
                                                                                                   aCCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTtgtg                                                  >  1:1323956/1‑150 (MQ=255)
                                                                                                    ccGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTgg                                                 <  2:533878/150‑1 (MQ=255)
                                                                                                    ccGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTgg                                                 <  1:1174433/150‑1 (MQ=255)
                                                                                                      gCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGaa                                               >  2:513310/1‑150 (MQ=255)
                                                                                                       ccTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGGTTGTGGaaa                                              <  1:1648335/150‑1 (MQ=255)
                                                                                                            cTGCTACATCTGCCGTAAAATCCTCGTCTGACGGCTACGCACGTAGCCCGATGGCGATGAGACTTTGATACGTAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTTGAAATAACg                                         <  1:105735/150‑1 (MQ=255)
                                                                                                              gATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGtt                                       <  1:703036/150‑1 (MQ=255)
                                                                                                                tACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTtgt                                     >  1:963147/1‑150 (MQ=255)
                                                                                                                 aCATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTtgtg                                    <  2:1646484/150‑1 (MQ=255)
                                                                                                                  cATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGc                                   >  2:1043524/1‑150 (MQ=255)
                                                                                                                  cATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGc                                   >  1:182207/1‑150 (MQ=255)
                                                                                                                  cATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGc                                   <  2:497398/150‑1 (MQ=255)
                                                                                                                        ccGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGc                             >  1:85813/1‑150 (MQ=255)
                                                                                                                         cGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGATACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCt                            <  2:1648871/150‑1 (MQ=255)
                                                                                                                         cGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCt                            >  2:752271/1‑150 (MQ=255)
                                                                                                                          gTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCtt                           <  1:200576/150‑1 (MQ=255)
                                                                                                                           tAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTc                          <  2:1698300/150‑1 (MQ=255)
                                                                                                                              aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGAc                       <  2:191271/150‑1 (MQ=255)
                                                                                                                              aaTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGAc                       <  2:1179023/150‑1 (MQ=255)
                                                                                                                               aTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACg                      <  2:1582401/150‑1 (MQ=255)
                                                                                                                               aTCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACg                      >  2:394580/1‑150 (MQ=255)
                                                                                                                                 ccACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGcc                    >  1:944416/1‑150 (MQ=255)
                                                                                                                                  cACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCa                   >  2:480171/1‑150 (MQ=255)
                                                                                                                                  cACCCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGACGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCa                   <  1:1094265/150‑1 (MQ=255)
                                                                                                                                    cGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCaaa                 >  2:445342/1‑150 (MQ=255)
                                                                                                                                      ccatcgggCTACGTGCGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAaca               >  2:1025815/7‑150 (MQ=255)
                                                                                                                                       catcgggCTACGTGCGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAacac              >  1:50436/6‑150 (MQ=255)
                                                                                                                                       catcgggCTACGTGCGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAacac              >  2:86918/6‑150 (MQ=255)
                                                                                                                                       cTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAacac              >  2:196293/1‑150 (MQ=255)
                                                                                                                                         tcgggCTACGTGCGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAacacac            >  1:1313466/4‑150 (MQ=255)
                                                                                                                                              cTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCg       >  2:762071/1‑150 (MQ=255)
                                                                                                                                              cTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCg       >  1:1255926/1‑150 (MQ=255)
                                                                                                                                               tACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTATTACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGc      <  2:459409/150‑1 (MQ=255)
                                                                                                                                                   cACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCaaa  >  2:134976/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATTGCCCTGCAGAAAGATCTGCAACCTCGTGAGAGCAAAACGCTGGCCCTCACTTCGCGCGAAGCTTAATCCAGCTGGTGTTGTTGCATAAACGGCTCACCGCCTAACTGATACATCTGCCGTAAAATCCACGCCTGACGGCTACGCACGTAGCCCGATGGCGAGGAGACTTTGAAACGAAGTGGATTAGGTAATACAGCTGCCAGTAATGCAGCTTCCGACCGGGTAAGTTTGCTCGCGGGTTTGTGGAAATAACGTTGTGCCGCAGCTTCGACGCCAAACACACCGTCGCCAAA  >  NC_000913/3349015‑3349311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: