Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,371,065 C→T 7.1% intergenic (‑29/+19) nanE ← / ← nanT putative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,371,0650CT 7.1% 483.0 / 16.3 154intergenic (‑29/+19)nanE/nanTputative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base C (63/80);  new base T (4/7);  total (67/87)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 155 total aligned reads displayed.

ATGCCATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCA  >  NC_000913/3370919‑3371214
                                                                                                                                                  |                                                                                                                                                     
aTGCCATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATa                                                                                                                                                    <  2:583/150‑1 (MQ=255)
     aTGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCg                                                                                                                                               <  1:1486841/150‑1 (MQ=255)
      tGGCGGCGACGATTTCGGGTTTCTCTAGCGGGCTGTCCGGAACCGGCGGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCgg                                                                                                                                              <  2:163749/150‑1 (MQ=255)
        gcggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                            <  1:1012327/150‑1 (MQ=255)
         cggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcc                                                                                                                                           <  2:1055815/150‑2 (MQ=255)
          ggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcct                                                                                                                                          <  2:1642101/150‑3 (MQ=255)
          ggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGCCCGGGCaa                                                                                                                                          >  2:1286999/1‑150 (MQ=255)
          ggcgACGATTTCGGGTTTATCGAGCGGGCGGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCTTTAGCAGCGATGTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGCTCTATGTACCGGGcct                                                                                                                                          <  1:134633/150‑3 (MQ=255)
            cgacgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACa                                                                                                                                        >  2:868986/1‑150 (MQ=255)
            cgacgaTTTCGGGGTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACACTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGCCCGGGCAACa                                                                                                                                        >  2:844131/1‑150 (MQ=255)
             gacgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCCGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAg                                                                                                                                       >  1:711812/1‑150 (MQ=255)
               cgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcctttcgc                                                                                                                                     <  1:853009/150‑8 (MQ=255)
               cgaTTGCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcctttcgc                                                                                                                                     <  1:1364101/150‑8 (MQ=255)
                gaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGAt                                                                                                                                    <  1:999618/150‑1 (MQ=255)
                     cGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACt                                                                                                                               >  1:574280/1‑150 (MQ=255)
                       ggTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACttt                                                                                                                             >  2:456917/1‑150 (MQ=255)
                       ggTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACttt                                                                                                                             >  2:1019554/1‑150 (MQ=255)
                        gTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACtttt                                                                                                                            <  2:1633470/150‑1 (MQ=255)
                        gTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACtttt                                                                                                                            <  2:1308215/150‑1 (MQ=255)
                               gAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTg                                                                                                                     <  2:1240714/150‑1 (MQ=255)
                                 gcgggcggTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGAc                                                                                                                   <  2:238628/150‑1 (MQ=255)
                                 gCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGAc                                                                                                                   <  2:175381/150‑1 (MQ=255)
                                  cGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACt                                                                                                                  <  1:1623991/150‑1 (MQ=255)
                                      cTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAAt                                                                                                              <  1:548278/150‑1 (MQ=255)
                                       tGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATc                                                                                                             <  2:92959/150‑1 (MQ=255)
                                              aaCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTg                                                                                                      <  2:193064/150‑1 (MQ=255)
                                               aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                     <  2:1338604/150‑1 (MQ=255)
                                               aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                     <  1:868986/150‑1 (MQ=255)
                                               aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                     >  1:1583416/1‑150 (MQ=255)
                                               aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                     <  2:200248/150‑1 (MQ=255)
                                                ccGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTCACGACATACATCTTCCCATAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgc                                                                                                    <  2:1089817/150‑1 (MQ=255)
                                                  ggCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTTACGACATACATCTTCCCTTAGCGTAAGGCCCGGTACATTGCGCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcgc                                                                                                  >  1:1135873/1‑150 (MQ=255)
                                                  ggCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcgc                                                                                                  <  2:1364553/150‑1 (MQ=255)
                                                   gCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGTTCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCt                                                                                                 >  2:302639/1‑150 (MQ=255)
                                                     tGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGc                                                                                               >  2:1486534/1‑150 (MQ=255)
                                                     tGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGc                                                                                               >  2:1583058/1‑150 (MQ=255)
                                                      ggCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGcc                                                                                              <  1:625131/150‑1 (MQ=255)
                                                      ggCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGcc                                                                                              <  2:955509/150‑1 (MQ=255)
                                                            agaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacgg                                                                                        >  1:1702381/1‑150 (MQ=255)
                                                            agaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCAGCGCCaaaaac                                                                                        <  1:415486/150‑4 (MQ=255)
                                                             gaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggc                                                                                       >  2:759571/1‑150 (MQ=255)
                                                             gaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCAGCGCCaaaaacg                                                                                       <  1:1019554/150‑5 (MQ=255)
                                                             gaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCAGCGCCaaaaacg                                                                                       <  1:456917/150‑5 (MQ=255)
                                                                aaTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggcacg                                                                                    <  1:464347/150‑1 (MQ=255)
                                                                 aTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                            >  2:1379431/1‑93 (MQ=255)
                                                                 aTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggcacgg                                                                                   <  1:485191/150‑1 (MQ=255)
                                                                     ggCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCAcc                                                                               >  1:1705586/1‑150 (MQ=255)
                                                                         aCCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTg                                                                           >  1:1401123/1‑150 (MQ=255)
                                                                            gTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAAt                                                                        >  2:799299/1‑150 (MQ=255)
                                                                            gTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAAt                                                                        <  1:986807/150‑1 (MQ=255)
                                                                                gcagcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGACAAACGGCACGACACCGCTGAATGGtt                                                                    >  1:1290700/1‑150 (MQ=255)
                                                                                 cagcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGttt                                                                   <  1:1489980/150‑1 (MQ=255)
                                                                                    cGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTAcc                                                                >  1:944820/1‑150 (MQ=255)
                                                                                     gATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCg                                                               >  1:1654188/1‑150 (MQ=255)
                                                                                        tttttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCg                                                            <  1:204078/150‑1 (MQ=255)
                                                                                         ttttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGa                                                           >  2:757197/1‑150 (MQ=255)
                                                                                         ttttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGa                                                           >  1:147711/1‑150 (MQ=255)
                                                                                           ttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATa                                                         <  1:1198668/150‑1 (MQ=255)
                                                                                           ttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATa                                                         <  2:1482940/150‑1 (MQ=255)
                                                                                            tGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAg                                                        >  1:770597/1‑150 (MQ=255)
                                                                                             gATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTATTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGc                                                       >  1:1432138/1‑150 (MQ=255)
                                                                                                ccAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTc                                                    <  2:442652/150‑1 (MQ=255)
                                                                                                 cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                   <  2:503864/150‑1 (MQ=255)
                                                                                                 cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                   <  2:1567899/150‑1 (MQ=255)
                                                                                                 cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                   >  2:809579/1‑150 (MQ=255)
                                                                                                  aGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCTGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCAt                                                  >  1:314781/1‑150 (MQ=255)
                                                                                                   gTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                 <  2:1554576/150‑1 (MQ=255)
                                                                                                    ttGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGa                                                <  2:1598942/150‑1 (MQ=255)
                                                                                                       tgCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTa                                             >  1:259342/1‑150 (MQ=255)
                                                                                                           aGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCa                                         >  1:246578/1‑150 (MQ=255)
                                                                                                               aCGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGc                                     <  2:1401123/150‑1 (MQ=255)
                                                                                                               aCGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGc                                     <  1:829644/150‑1 (MQ=255)
                                                                                                                 gACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                 <  1:513720/136‑1 (MQ=255)
                                                                                                                 gACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                 >  2:513720/1‑136 (MQ=255)
                                                                                                                  acatacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTc                                  >  1:1677693/1‑150 (MQ=255)
                                                                                                                  acatacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTcc                                 >  2:31071/1‑150 (MQ=255)
                                                                                                                   catacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTcc                                 >  2:1358130/1‑150 (MQ=255)
                                                                                                                    atacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCg                                <  2:574280/150‑1 (MQ=255)
                                                                                                                      acatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCggg                              <  2:1702381/150‑1 (MQ=255)
                                                                                                                       catCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTACGGgc                             >  1:267023/1‑150 (MQ=255)
                                                                                                                        atCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTAAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGgcg                            >  2:1527893/1‑150 (MQ=255)
                                                                                                                         tCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGgcgc                           <  1:1286999/150‑1 (MQ=255)
                                                                                                                             cccTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGAGTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaacc                       >  1:734069/1‑150 (MQ=255)
                                                                                                                             cccTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaacc                       >  2:411821/1‑150 (MQ=255)
                                                                                                                                ttAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaaccaac                    <  1:1462389/150‑1 (MQ=255)
                                                                                                                                  aGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGc                  <  1:1583058/150‑1 (MQ=255)
                                                                                                                                   gCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCt                 <  2:944820/150‑1 (MQ=255)
                                                                                                                                   gCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCt                 <  1:799299/150‑1 (MQ=255)
                                                                                                                                    cGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTg                >  2:1155780/1‑150 (MQ=255)
                                                                                                                                      aaaGGCCCGGTACATAGACCGTGCAACAGGATTAACTTTTGGTTGTGACTAAATCGTTTTTGGCGATGACAAACGGCACGGCTCCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGaa              >  2:1630359/1‑150 (MQ=255)
                                                                                                                                       gttgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAc             >  1:827563/4‑150 (MQ=255)
                                                                                                                                        aGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcg            <  1:1377814/150‑1 (MQ=255)
                                                                                                                                        aGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcg            >  2:1662594/1‑150 (MQ=255)
                                                                                                                                        aGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGATTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcg            <  2:700674/150‑1 (MQ=255)
                                                                                                                                         tgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCATCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgc           >  1:671319/2‑150 (MQ=255)
                                                                                                                                         ggCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgc           >  2:1104246/1‑150 (MQ=255)
                                                                                                                                          gCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgcg          <  1:1104246/150‑1 (MQ=255)
                                                                                                                                              ggTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCAGGGCGCAACCAACGCTGAACGCGAGaa      >  1:346041/1‑150 (MQ=255)
                                                                                                                                               gTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAg     >  1:1245558/1‑150 (MQ=255)
                                                                                                                                               gTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAgg    <  1:31071/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATGCCATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCA  >  NC_000913/3370919‑3371214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: