Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,371,069 G→T 6.6% intergenic (‑33/+15) nanE ← / ← nanT putative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,371,0690GT 6.6% 489.0 / 16.2 158intergenic (‑33/+15)nanE/nanTputative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (67/80);  new base T (4/7);  total (71/87)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 160 total aligned reads displayed.

CATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATC  >  NC_000913/3370923‑3371218
                                                                                                                                                  |                                                                                                                                                     
  tGGCGGCGACGATTTCGGGTTTCTCTAGCGGGCTGTCCGGAACCGGCGGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCgg                                                                                                                                                  <  2:163749/150‑1 (MQ=255)
    gcggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  1:1012327/150‑1 (MQ=255)
    gcggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  1:628600/150‑1 (MQ=255)
     cggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTCTATGTACCGGGcc                                                                                                                                               <  2:1055815/150‑2 (MQ=255)
      ggcgACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGCCCGGGCaa                                                                                                                                              >  2:1286999/1‑150 (MQ=255)
      ggcgACGATTTCGGGTTTATCGAGCGGGCGGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCTTTAGCAGCGATGTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGCTCTATGTACCGGGcct                                                                                                                                              <  1:134633/150‑3 (MQ=255)
        cgacgaTTTCGGGGTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACACTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGCCCGGGCAACa                                                                                                                                            >  2:844131/1‑150 (MQ=255)
         gacgaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCCGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAg                                                                                                                                           >  1:711812/1‑150 (MQ=255)
           cgaTTTCGGGTTTATCGAGCGGGCTATCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTATCGAAAGGCCCGGTCTATGTACCGGGcctttcgc                                                                                                                                         <  1:555101/150‑8 (MQ=255)
            gaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGAt                                                                                                                                        <  1:999618/150‑1 (MQ=255)
            gaTTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGAt                                                                                                                                        <  2:1555571/150‑1 (MQ=255)
              tttCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTa                                                                                                                                      <  1:1702177/150‑1 (MQ=255)
                 cGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACt                                                                                                                                   >  1:574280/1‑150 (MQ=255)
                   ggTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACttt                                                                                                                                 >  1:1600827/1‑150 (MQ=255)
                   ggTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACttt                                                                                                                                 >  2:456917/1‑150 (MQ=255)
                   ggTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACttt                                                                                                                                 >  2:1019554/1‑150 (MQ=255)
                    gTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACtttt                                                                                                                                <  2:1595105/150‑1 (MQ=255)
                    gTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACtttt                                                                                                                                <  2:1308215/150‑1 (MQ=255)
                     tttATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTGATTCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTgg                                                                                                                              <  1:805495/150‑1 (MQ=255)
                        aTCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGtt                                                                                                                            >  2:309751/1‑150 (MQ=255)
                           gAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTg                                                                                                                         <  2:1240714/150‑1 (MQ=255)
                              cGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACt                                                                                                                      <  2:1600827/150‑1 (MQ=255)
                                   tGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATc                                                                                                                 <  2:92959/150‑1 (MQ=255)
                                   tGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATc                                                                                                                 >  1:17114/1‑150 (MQ=255)
                                         gAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGttttt                                                                                                           <  1:1376503/150‑1 (MQ=255)
                                          aaCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTg                                                                                                          <  2:193064/150‑1 (MQ=255)
                                           aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                         <  2:206326/150‑1 (MQ=255)
                                           aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                         <  1:868986/150‑1 (MQ=255)
                                           aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                         <  2:1338604/150‑1 (MQ=255)
                                           aCCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTgg                                                                                                         >  1:1583416/1‑150 (MQ=255)
                                            ccGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTCACGACATACATCTTCCCATAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgc                                                                                                        <  2:1089817/150‑1 (MQ=255)
                                              ggCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTTACGACATACATCTTCCCTTAGCGTAAGGCCCGGTACATTGCGCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcgc                                                                                                      >  1:1135873/1‑150 (MQ=255)
                                              ggCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGgcgc                                                                                                      <  2:1364553/150‑1 (MQ=255)
                                               gCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGTTCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCt                                                                                                     >  2:302639/1‑150 (MQ=255)
                                                 tGGCAGGAGACAATCCGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTATATCGTTTTTGGCGCTGc                                                                                                   <  2:196411/150‑1 (MQ=255)
                                                  ggCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGcc                                                                                                  <  2:955509/150‑1 (MQ=255)
                                                  ggCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGcc                                                                                                  >  1:1442924/1‑150 (MQ=255)
                                                        agaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCAGCGCCaaaaac                                                                                            <  1:415486/150‑4 (MQ=255)
                                                         gaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggc                                                                                           >  2:759571/1‑150 (MQ=255)
                                                         gaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCAGCGCCaaaaacg                                                                                           <  1:1019554/150‑5 (MQ=255)
                                                         gaCAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCAGCGCCaaaaacg                                                                                           <  1:456917/150‑5 (MQ=255)
                                                            aaTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggcacg                                                                                        <  1:464347/150‑1 (MQ=255)
                                                             aTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                <  1:1379431/93‑1 (MQ=255)
                                                             aTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGc                                                                                                                                                >  2:1379431/1‑93 (MQ=255)
                                                             aTCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAacggcacgg                                                                                       <  1:485191/150‑1 (MQ=255)
                                                                 ggCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCAcc                                                                                   <  2:1526457/150‑1 (MQ=255)
                                                                 ggCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCAcc                                                                                   >  1:1705586/1‑150 (MQ=255)
                                                                     aCCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTg                                                                               >  1:1401123/1‑150 (MQ=255)
                                                                        gTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAAt                                                                            <  1:986807/150‑1 (MQ=255)
                                                                        gTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAAt                                                                            >  2:799299/1‑150 (MQ=255)
                                                                             cagcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGttt                                                                       <  1:1489980/150‑1 (MQ=255)
                                                                             cagcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGttt                                                                       >  1:1554013/1‑150 (MQ=255)
                                                                             cagcGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGttt                                                                       <  1:133553/150‑1 (MQ=255)
                                                                                cGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTAcc                                                                    >  1:944820/1‑150 (MQ=255)
                                                                                     ttttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGa                                                               >  2:757197/1‑150 (MQ=255)
                                                                                       ttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATa                                                             <  1:1198668/150‑1 (MQ=255)
                                                                                       ttGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATa                                                             <  2:1482940/150‑1 (MQ=255)
                                                                                        tGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAg                                                            >  1:770597/1‑150 (MQ=255)
                                                                                            ccAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTc                                                        <  1:844131/150‑1 (MQ=255)
                                                                                             cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                       <  2:503864/150‑1 (MQ=255)
                                                                                             cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                       >  2:809579/1‑150 (MQ=255)
                                                                                             cAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCa                                                       <  2:1567899/150‑1 (MQ=255)
                                                                                              aGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCTGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCAt                                                      >  1:314781/1‑150 (MQ=255)
                                                                                               gTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                     <  1:1286980/150‑1 (MQ=255)
                                                                                                 tgtgCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAg                                                   <  1:1486534/150‑1 (MQ=255)
                                                                                                   tgCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTa                                                 >  1:259342/1‑150 (MQ=255)
                                                                                                       aGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCa                                             >  1:246578/1‑150 (MQ=255)
                                                                                                         tAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATTGCGTCATGAGTACGCAAc                                           <  2:1635113/150‑1 (MQ=255)
                                                                                                           aCGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGc                                         <  2:1401123/150‑1 (MQ=255)
                                                                                                             gACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                     >  2:513720/1‑136 (MQ=255)
                                                                                                             gACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATg                                                     <  1:513720/136‑1 (MQ=255)
                                                                                                              acatacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTc                                      >  1:1677693/1‑150 (MQ=255)
                                                                                                               catacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTcc                                     >  2:1358130/1‑150 (MQ=255)
                                                                                                                atacatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCg                                    <  2:574280/150‑1 (MQ=255)
                                                                                                                  acatCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCggg                                  <  2:1702381/150‑1 (MQ=255)
                                                                                                                   catCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTACGGgc                                 >  1:267023/1‑150 (MQ=255)
                                                                                                                    atCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTAAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGgcg                                >  2:1527893/1‑150 (MQ=255)
                                                                                                                     tCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGgcgc                               <  1:1286999/150‑1 (MQ=255)
                                                                                                                         cccTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaacc                           >  2:411821/1‑150 (MQ=255)
                                                                                                                            ttAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGcaaccaac                        <  1:1462389/150‑1 (MQ=255)
                                                                                                                               gCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCt                     <  1:759571/150‑1 (MQ=255)
                                                                                                                               gCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCt                     <  2:944820/150‑1 (MQ=255)
                                                                                                                                  aaaGGCCCGGTACATAGACCGTGCAACAGGATTAACTTTTGGTTGTGACTAAATCGTTTTTGGCGATGACAAACGGCACGGCTCCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGaa                  >  2:1630359/1‑150 (MQ=255)
                                                                                                                                   gttgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAc                 >  1:827563/4‑150 (MQ=255)
                                                                                                                                   gttgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAc                 >  1:1642718/4‑150 (MQ=255)
                                                                                                                                    aGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGATTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcg                <  2:700674/150‑1 (MQ=255)
                                                                                                                                     tgCCCGGTCTATGTACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCATCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgc               >  1:671319/2‑150 (MQ=255)
                                                                                                                                     ggCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgc               >  2:1104246/1‑150 (MQ=255)
                                                                                                                                      gCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAAcgcg              <  1:1104246/150‑1 (MQ=255)
                                                                                                                                       cccGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAAGCGTTTTTGGCGCTGCCAAACGGCACGGCCCCGCTGAATGGTTTACCGTCGATAGCGTCAAGAGTACGCAACGCTTCCGGTCGCAACCAAACCTGAACGCga             >  1:88531/1‑150 (MQ=255)
                                                                                                                                        ccGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCgag            >  2:1661227/1‑150 (MQ=255)
                                                                                                                                          ggTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCAGGGCGCAACCAACGCTGAACGCGAGaa          >  1:346041/1‑150 (MQ=255)
                                                                                                                                          ggTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACCGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGaa          >  2:437356/1‑150 (MQ=255)
                                                                                                                                           gTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAgg        <  1:31071/150‑1 (MQ=255)
                                                                                                                                              cATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGACGGCa      >  2:1715590/1‑150 (MQ=255)
                                                                                                                                               aTAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCat     >  2:1195146/1‑150 (MQ=255)
                                                                                                                                                tAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCAAGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCata    >  2:115509/1‑150 (MQ=255)
                                                                                                                                                 aGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCatat   <  2:555309/150‑1 (MQ=255)
                                                                                                                                                  gACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATc  >  2:887816/1‑150 (MQ=255)
                                                                                                                                                  gACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATc  >  2:909996/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CATGGCGGCGACGATTTCGGGTTTATCGAGCGGGCTGTCCGGAACCGGCTGGCAGGAGACAATCAGGCCACCGTTAGCAGCGATTTTTTGATCCAGTTGTGCAAGTAACGACATACATCTTCCCTTAGCGAAAGGCCCGGTACATAGACCGGGCAACAGGATTAACTTTTGGTTTTGACTAAATCGTTTTTGGCGCTGCCAAACGGCACGGCACCGCTGAATGGTTTACCGTCGATAGCGTCATGAGTACGCAACGCTTCCGGGCGCAACCAACGCTGAACGCGAGAAGGCATATC  >  NC_000913/3370923‑3371218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: