Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,377,771 A→G 7.0% intergenic (‑351/+44) sspA ← / ← rpsI stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,377,7710AG 7.0% 283.0 / 9.7 101intergenic (‑351/+44)sspA/rpsIstringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog/30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base A (52/40);  new base G (5/2);  total (57/44)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 104 total aligned reads displayed.

GAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCA  >  NC_000913/3377622‑3377920
                                                                                                                                                      |                                                                                                                                                     
gAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGaa                                                                                                                                                       >  2:1270209/1‑150 (MQ=255)
  aaaaGAGATGCGCTTTAGTCTGAAATATTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGc                                                                                                                                                     <  1:861053/150‑1 (MQ=255)
     agagATGCGCTTTAGTCTGAAATAGTTGTCTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCggg                                                                                                                                                  <  1:12656/150‑1 (MQ=255)
     agagATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCggg                                                                                                                                                  >  2:1556505/1‑150 (MQ=255)
     agagATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCggg                                                                                                                                                  <  1:1325746/150‑1 (MQ=255)
      gagaTGCGCTTTAGTCTTAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGt                                                                                                                                                 <  2:237891/150‑1 (MQ=255)
      gagaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGt                                                                                                                                                 <  2:855843/150‑1 (MQ=255)
      gagaTGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGt                                                                                                                                                 >  2:1204991/1‑150 (MQ=255)
          tGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttttt                                                                                                                                             <  2:1228902/150‑1 (MQ=255)
          tGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGttttt                                                                                                                                             >  1:854433/1‑150 (MQ=255)
            cgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGttttttt                                                                                                                                           <  2:56436/150‑2 (MQ=255)
            cgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTc                                                                                                                                           <  1:1621217/150‑1 (MQ=255)
            cgcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATAGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTc                                                                                                                                           <  1:1081929/150‑1 (MQ=255)
             gcTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCAGACGAAGCGGGTTTTTTCg                                                                                                                                          >  1:1099144/1‑150 (MQ=255)
              cTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGa                                                                                                                                         <  2:470974/150‑1 (MQ=255)
                 tAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCTTCGGCGGGtttttttatagc                                                                                                                                      <  1:1354542/150‑7 (MQ=255)
                   gTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAAt                                                                                                                                     >  1:585437/1‑150 (MQ=255)
                       gAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGttt                                                                                                                                >  1:939417/1‑150 (MQ=255)
                          aTAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTAATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCt                                                                                                                             <  1:827045/150‑1 (MQ=255)
                               tGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCgg                                                                                                                        <  2:729114/150‑1 (MQ=255)
                                gACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGa                                                                                                                       >  2:416801/1‑150 (MQ=255)
                                    tattCCCTTCTTGGCGATGTGGTGTTGGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGATTTTACGCTGATTCAGTTTTTAGATATAAAAAAACCCGCCGATGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAg                                                                                                                   <  1:236484/147‑1 (MQ=255)
                                    tAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAg                                                                                                                   >  1:125287/1‑150 (MQ=255)
                                        cccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATGAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGc                                                                                                               <  1:1486911/150‑1 (MQ=255)
                                         ccTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc                                                                                                              <  1:1327645/150‑1 (MQ=255)
                                          cTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGcc                                                                                                              <  2:585437/150‑1 (MQ=255)
                                            tATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAAt                                                                                                           <  2:139014/150‑1 (MQ=255)
                                            tATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCAGGAGCAGAAGCCAAt                                                                                                           >  1:538329/1‑150 (MQ=255)
                                             aTTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAAtt                                                                                                          >  1:1140685/1‑150 (MQ=255)
                                              ttGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTa                                                                                                         <  1:416801/150‑1 (MQ=255)
                                                  cGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGt                                                                                                     >  2:1029215/1‑150 (MQ=255)
                                                   gATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGtt                                                                                                    <  2:854433/150‑1 (MQ=255)
                                                       tgGTTTTTGTTTTACCTGTCTGTCAGGTTGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTATCGTTTGga                                                                                                <  1:1085081/150‑1 (MQ=255)
                                                       tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                                <  1:248622/150‑1 (MQ=255)
                                                       tgGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGga                                                                                                <  2:1375707/150‑1 (MQ=255)
                                                        ggTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag                                                                                               >  1:1419607/1‑150 (MQ=255)
                                                        ggTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgag                                                                                               >  2:472094/1‑150 (MQ=255)
                                                         gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAATCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgaga                                                                                              <  1:468015/150‑1 (MQ=255)
                                                         gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGgaga                                                                                              <  2:4839/150‑1 (MQ=255)
                                                         gTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGGTTTTACGCTGATTCAGATTTTTGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCTGAGCAGAAGCCAATTAACGTTTgggta                                                                                              >  1:674736/1‑147 (MQ=255)
                                                          tttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTTACGTTTGGAGaa                                                                                             >  2:588844/1‑150 (MQ=255)
                                                           ttttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTATTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAAc                                                                                            <  1:1052424/150‑1 (MQ=255)
                                                            tttGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACt                                                                                           <  2:1206344/150‑1 (MQ=255)
                                                               gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCg                                                                                        >  1:1639066/1‑150 (MQ=255)
                                                               gTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGACGAAGCAGAAGCAAATTAACGTTTGGAGAACTgag                                                                                        >  1:331960/1‑148 (MQ=255)
                                                                ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGaa                                                                                                                 >  1:1352144/1‑124 (MQ=255)
                                                                ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGaa                                                                                                                 <  2:1352144/124‑1 (MQ=255)
                                                                ttttACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCgg                                                                                       >  2:1300157/1‑150 (MQ=255)
                                                                   tACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacg                                                                                    <  2:6740/150‑1 (MQ=255)
                                                                    aCCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacga                                                                                   >  1:826171/1‑150 (MQ=255)
                                                                     ccTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGgacgac                                                                                  >  2:108943/1‑150 (MQ=255)
                                                                        gtctgtcAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAAGTGCGgacgacgac                                                                               >  2:1523176/1‑150 (MQ=255)
                                                                           tgtcAGGTGGCAGCAAAAAGCAACTTTCCAGGTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTg                                                                            <  1:1067727/150‑1 (MQ=255)
                                                                               aGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCttt                                                                        >  2:1266260/1‑150 (MQ=255)
                                                                                ggTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTa                                                                       >  1:433111/1‑150 (MQ=255)
                                                                                ggTGGCAGCAAAAAGCAACTTTCCAGATTTAACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTa                                                                       <  2:416360/150‑1 (MQ=255)
                                                                                   ggCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGc                                                                    >  1:1408517/1‑150 (MQ=255)
                                                                                    gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACgaa                                                                   >  1:149842/1‑148 (MQ=255)
                                                                                    gcagcaAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCa                                                                   <  2:1059627/150‑1 (MQ=255)
                                                                                        caAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCTGAGCAGAAGCCAATTAACGTTTGGAGAACTGCTGACGACGACGTGCTTTACGCAGAcc                                                               >  1:1106008/1‑150 (MQ=255)
                                                                                           aaaGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGAc                                                            >  2:1643939/1‑150 (MQ=255)
                                                                                             aGCAACTTTCCAGTATTTACGCTTATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCAGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACtt                                                          >  2:372285/1‑150 (MQ=255)
                                                                                               cAACATTCCATTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTc                                                        <  1:620346/150‑1 (MQ=255)
                                                                                                aaCTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCt                                                       >  1:1010016/1‑150 (MQ=255)
                                                                                                  cTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTATTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTa                                                     >  2:802238/1‑150 (MQ=255)
                                                                                                          tttttACGCTGATTCAGATTTTAGCTCTAAAAAAACCCGCCGACGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTACCGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAc                                             <  1:270583/150‑1 (MQ=255)
                                                                                                           ttttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAAcc                                            <  1:583310/150‑1 (MQ=255)
                                                                                                            tttACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCt                                           >  2:1454131/1‑150 (MQ=255)
                                                                                                                cGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACg                                       >  1:739862/1‑150 (MQ=255)
                                                                                                                cGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACg                                       >  2:1441047/1‑150 (MQ=255)
                                                                                                                cGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACg                                       >  1:644797/1‑150 (MQ=255)
                                                                                                                    gATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCg                                   >  2:1548489/1‑150 (MQ=255)
                                                                                                                     aTTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGt                                  >  1:932776/1‑150 (MQ=255)
                                                                                                                     aTTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGt                                  >  1:696073/1‑150 (MQ=255)
                                                                                                                      ttCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTc                                 >  1:779910/1‑150 (MQ=255)
                                                                                                                       tCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGCCGACGTGCTTTACGCAGCCCGACTTTCTTTCGTTCATCCTGACGAGCGTCa                                >  2:1520666/1‑150 (MQ=255)
                                                                                                                        cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGTAGCAGAGGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACTTTCAACCTGACGAGCGTCAc                               >  2:1504657/1‑150 (MQ=255)
                                                                                                                        cAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCAc                               <  1:1029215/150‑1 (MQ=255)
                                                                                                                         aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACg                              >  1:146209/1‑150 (MQ=255)
                                                                                                                         aGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACg                              <  1:1655479/150‑1 (MQ=255)
                                                                                                                          gATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGa                             >  1:651239/1‑150 (MQ=255)
                                                                                                                           aTTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAg                            <  1:1643939/150‑1 (MQ=255)
                                                                                                                            tttaatcTATAAAAAAACCCGCCGAAGAGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGAAGAAGCCAATTAACGTTTGGAGAAATGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGt                           <  2:645046/144‑1 (MQ=255)
                                                                                                                             tttAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTa                          >  1:1469713/1‑150 (MQ=255)
                                                                                                                              ttAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTaa                         <  1:1548489/150‑1 (MQ=255)
                                                                                                                                       gaaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCa                 >  1:1633560/2‑150 (MQ=255)
                                                                                                                                       gaaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCa                 >  2:1285536/2‑150 (MQ=255)
                                                                                                                                       aaaaacaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCa                 <  1:449858/150‑1 (MQ=255)
                                                                                                                                        aaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGTGGACGCCGACGTGCTTTACGCAGACCCACTTTCTTACGTTCAACCTGACGCGCGTCACGAGTAACgccgcccg                >  2:106443/1‑143 (MQ=255)
                                                                                                                                        aaaaaaCCCGCTTCGGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAg                >  1:241759/1‑150 (MQ=255)
                                                                                                                                         aaaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTTACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGc               <  1:588844/150‑1 (MQ=255)
                                                                                                                                          aaaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCt              >  1:142279/1‑150 (MQ=255)
                                                                                                                                           aaaCCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGCCCGACTTTCTTACGTTCAACCTGCCGAGCGTCACGAGTAACGAAGCCAGCtt             >  1:329271/1‑150 (MQ=255)
                                                                                                                                            aaCCCGCCGAAGCGGGGTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGCCCTGCGGACGACGACGTGCGTTAAGCAGACCGACTTTCTTACGTTCATCCTGACGCGCGTCACGAGTAACGAAGCCAGCgtt            >  1:1446475/1‑150 (MQ=255)
                                                                                                                                              cccGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTAc          >  1:666526/1‑150 (MQ=255)
                                                                                                                                                cgccgAAGCGGGTTTTTTCGAAAATTTTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGc        <  1:802238/150‑1 (MQ=255)
                                                                                                                                                cgccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGc        <  2:736478/150‑1 (MQ=255)
                                                                                                                                                cgccgAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGAAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGCCGTGCTTTACGCAGACCGACTTTCTTACGTTCACCCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGc        >  1:1276416/1‑150 (MQ=255)
                                                                                                                                                      aGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCa  <  2:149842/150‑1 (MQ=255)
                                                                                                                                                      aGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCa  <  2:1010016/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GAAAAAGAGATGCGCTTTAGTCTGAAATAGTTGACTTAGTCCCTTATTGGCGATGTGGTTTTTGTTTTACCTGTCTGTCAGGTGGCAGCAAAAAGCAACTTTCCAGTTTTTACGCTGATTCAGATTTTAGCTATAAAAAAACCCGCCGAAGCGGGTTTTTTCGAAAATTGTTTTCTGCCGGAGCAGAAGCCAATTAACGTTTGGAGAACTGCGGACGACGACGTGCTTTACGCAGACCGACTTTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCA  >  NC_000913/3377622‑3377920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: