Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,380,440 C→A 5.4% L84M (CTG→ATG) ‡ yhcB → DUF1043 family inner membrane‑anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,380,4400CA 5.4% 307.6 / 5.2 92L84M (CTG→ATG) ‡yhcBDUF1043 family inner membrane‑anchored protein
Reads supporting (aligned to +/- strand):  ref base C (42/45);  new base A (2/3);  total (44/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACT  >  NC_000913/3380292‑3380587
                                                                                                                                                    |                                                                                                                                                   
gCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCct                                                                                                                                                    >  2:778187/1‑150 (MQ=255)
 cAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctg                                                                                                                                                   <  2:418814/150‑1 (MQ=255)
 cAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctg                                                                                                                                                   >  2:488085/1‑150 (MQ=255)
  aGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgc                                                                                                                                                  <  2:277938/150‑1 (MQ=255)
  aGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATGACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgc                                                                                                                                                  >  2:340165/1‑150 (MQ=255)
   gTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgct                                                                                                                                                 >  2:258195/1‑150 (MQ=255)
    tACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgctg                                                                                                                                                <  2:1172977/150‑1 (MQ=255)
    tACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgctg                                                                                                                                                <  1:1258179/150‑1 (MQ=255)
      cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc                                                                                                                                              <  1:968610/150‑1 (MQ=255)
      cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc                                                                                                                                              <  2:1485676/150‑1 (MQ=255)
      cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc                                                                                                                                              <  2:1652416/150‑1 (MQ=255)
      cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGAGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCCGCAGCCTGCTGcc                                                                                                                                              >  2:1552556/1‑150 (MQ=255)
      cGAAATGGAAATGAATTAAGCTTAAATGGTCGAGTATCGCTAAGAGCTGGTTAGCCACTTTTCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATGAGCACATGGCAAAAATCTCCAGCAGCCTGCTGcc                                                                                                                                              <  1:331021/150‑1 (MQ=255)
       gAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCGCGCCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                             >  2:1071557/1‑150 (MQ=255)
       gAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                             <  1:1526896/150‑1 (MQ=255)
        aaCTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCgg                                                                                                                                            <  1:379937/150‑1 (MQ=255)
          cTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGGTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGag                                                                                                                                          <  1:876706/150‑2 (MQ=255)
           tGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAAc                                                                                                                                         <  1:1570509/150‑1 (MQ=255)
           tGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCAGGCTGCTGGagc                                                                                                                                         <  1:189013/150‑3 (MQ=255)
             gAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTg                                                                                                                                       <  1:1335367/150‑1 (MQ=255)
                aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                                    >  2:1158271/1‑150 (MQ=255)
                  gAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTctgc                                                                                                                                  >  2:537134/1‑150 (MQ=255)
                  caaTAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATCCCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGAGCTTTttgc                                                                                                                                  <  1:1416566/149‑1 (MQ=255)
                       aaGCTGAACTAGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAg                                                                                                                             >  1:1531243/1‑150 (MQ=255)
                          cTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaa                                                                                                                          <  2:845865/150‑1 (MQ=255)
                           tGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                         >  1:31576/1‑150 (MQ=255)
                           tGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                         >  1:1135569/1‑150 (MQ=255)
                            gAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAAc                                                                                                                        >  1:929987/1‑150 (MQ=255)
                             aaCTAGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAAcc                                                                                                                       <  2:1531243/150‑1 (MQ=255)
                                 ggACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                                   >  2:472590/1‑150 (MQ=255)
                                 ggACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                                   <  1:823075/150‑1 (MQ=255)
                                   aCGAGTAGCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTcc                                                                                                                 <  2:14519/150‑1 (MQ=255)
                                         aTCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATc                                                                                                           >  2:777730/1‑150 (MQ=255)
                                          tCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCg                                                                                                          >  2:791973/1‑150 (MQ=255)
                                             cgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCt                                                                                                       >  1:1597363/1‑150 (MQ=255)
                                              gAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTg                                                                                                      >  1:1250658/1‑150 (MQ=255)
                                                   gCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCACACCCGTTCCGTAATCGTCTGGCaga                                                                                                 >  2:333222/1‑150 (MQ=255)
                                                    cTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGCCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCagag                                                                                                >  2:833936/1‑150 (MQ=255)
                                                    cTGGTTAGACACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCagag                                                                                                >  1:251470/1‑150 (MQ=255)
                                                     tGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGt                                                                                               <  1:1307091/150‑1 (MQ=255)
                                                      ggTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTGCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGtg                                                                                              >  2:1390397/1‑149 (MQ=255)
                                                      ggTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTc                                                                                              <  2:811295/150‑1 (MQ=255)
                                                            ccACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                                        <  1:664485/150‑1 (MQ=255)
                                                             cACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                                       >  1:552296/1‑150 (MQ=255)
                                                             cACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                                       <  2:469236/150‑1 (MQ=255)
                                                             cACTTTGCCCGCAGCGCGGAATTACTGGAGACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                                       <  1:1710057/150‑1 (MQ=255)
                                                               cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCCCGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAg                                                                                     <  2:1426326/150‑1 (MQ=255)
                                                               cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAg                                                                                     <  1:89139/150‑1 (MQ=255)
                                                                        cAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                            <  1:48512/150‑1 (MQ=255)
                                                                          gcgcTGAATTACTGGATACCATGGCGCACGACTATCGCGAGCTGTATCAGCACATGTCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAAGCCGTTCCGTAAGCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAgg                                                                          <  1:1220643/150‑1 (MQ=255)
                                                                          gcgcGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAgg                                                                          >  2:1658205/1‑150 (MQ=255)
                                                                           cgcgGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGc                                                                         <  1:994739/150‑1 (MQ=255)
                                                                           cgcgGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGc                                                                         <  1:310950/150‑1 (MQ=255)
                                                                              ggAATTACTGGATACCATGGCGCGCGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCAcc                                                                      <  1:791056/150‑1 (MQ=255)
                                                                                 aTTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGt                                                                   >  1:1659485/1‑150 (MQ=255)
                                                                                  ttACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTg                                                                  <  2:875915/150‑1 (MQ=255)
                                                                                  ttACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTg                                                                  <  1:258195/150‑1 (MQ=255)
                                                                                  ttACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTg                                                                  <  1:1593515/150‑1 (MQ=255)
                                                                                   tACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGc                                                                 <  2:1135569/150‑1 (MQ=255)
                                                                                   tACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGc                                                                 <  1:1556197/150‑1 (MQ=255)
                                                                                    aCTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCa                                                                >  2:979333/1‑150 (MQ=255)
                                                                                          tACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGcc                                                          <  2:1597363/150‑1 (MQ=255)
                                                                                           aCCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCt                                                         <  1:488085/150‑1 (MQ=255)
                                                                                            ccATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTc                                                        >  1:552965/1‑150 (MQ=255)
                                                                                            ccATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTc                                                        >  1:559752/1‑150 (MQ=255)
                                                                                                  cgcACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCCGCAGCCTGCTGCCGGACCTGTCTGCTGCAGCAACCCCGTGCCGTCATCGTCTGGCAGCGTCTGAAGCCAGCAACGATAAGGCACCGGTGCAGATGCCTCGCGACt                                                  >  1:923295/1‑150 (MQ=255)
                                                                                                  cgcACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACt                                                  >  2:738316/1‑150 (MQ=255)
                                                                                                      cGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTc                                              <  1:666740/150‑1 (MQ=255)
                                                                                                      cGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGCAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTc                                              >  2:1693952/1‑150 (MQ=255)
                                                                                                       gACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCt                                             <  2:221520/150‑1 (MQ=255)
                                                                                                             cGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgc                                       <  1:778187/150‑1 (MQ=255)
                                                                                                              gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                      >  1:623230/1‑150 (MQ=255)
                                                                                                              gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                      >  2:1672008/1‑150 (MQ=255)
                                                                                                              gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                      <  2:1255193/150‑1 (MQ=255)
                                                                                                              gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAACCCAGCAACGATCAGGCCCCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                      >  1:1648402/1‑150 (MQ=255)
                                                                                                              cccAGCTGTATCAGCACATGCCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGACGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                      <  2:41189/149‑1 (MQ=255)
                                                                                                               ccAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcgc                                     >  2:1484061/1‑150 (MQ=255)
                                                                                                                cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCa                                    <  1:340165/150‑1 (MQ=255)
                                                                                                                  gCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATc                                  >  1:358644/1‑150 (MQ=255)
                                                                                                                          aGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgc                          >  1:1021677/1‑150 (MQ=255)
                                                                                                                          aGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgc                          <  2:1659485/150‑1 (MQ=255)
                                                                                                                          aGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGAAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgc                          <  2:251470/150‑1 (MQ=255)
                                                                                                                            cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgctg                        >  1:765250/1‑150 (MQ=255)
                                                                                                                              caTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCg                      >  1:173519/1‑150 (MQ=255)
                                                                                                                                tGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTa                    <  1:738316/150‑1 (MQ=255)
                                                                                                                                 ggCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                              <  2:1600325/107‑1 (MQ=255)
                                                                                                                                 ggCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                              >  1:1600325/1‑107 (MQ=255)
                                                                                                                                  gCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACt                  >  2:705471/1‑150 (MQ=255)
                                                                                                                                   cAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTg                 >  2:1665860/1‑150 (MQ=255)
                                                                                                                                   cAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTg                 >  2:1720034/1‑150 (MQ=255)
                                                                                                                                    aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCGGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTgg                <  2:1175254/150‑1 (MQ=255)
                                                                                                                                    aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTgg                <  2:552965/150‑1 (MQ=255)
                                                                                                                                    aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTgg                <  2:559752/150‑1 (MQ=255)
                                                                                                                                     gacagttCCGGCAGCAGGCTGCTGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTAGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGgc               >  1:1360458/7‑150 (MQ=255)
                                                                                                                                         gttCCGGCAGCAGGCTGCTGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGa           >  1:1244853/3‑150 (MQ=255)
                                                                                                                                          cTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGaa          >  1:1225561/1‑150 (MQ=255)
                                                                                                                                               gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGcgcg     >  2:1546907/1‑150 (MQ=255)
                                                                                                                                               gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGcgcg     >  2:997444/1‑150 (MQ=255)
                                                                                                                                                 agcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGAc   <  2:31576/150‑1 (MQ=255)
                                                                                                                                                  gcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGACCTGCTGCGTACTGGCGCGAAGCGCGACt  >  1:1348144/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACT  >  NC_000913/3380292‑3380587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: