Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,380,447 C→T 5.6% P86L (CCG→CTG)  yhcB → DUF1043 family inner membrane‑anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,380,4470CT 5.6% 284.6 / 2.7 88P86L (CCG→CTG) yhcBDUF1043 family inner membrane‑anchored protein
Reads supporting (aligned to +/- strand):  ref base C (42/41);  new base T (2/3);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTT  >  NC_000913/3380298‑3380596
                                                                                                                                                     |                                                                                                                                                     
cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc                                                                                                                                                       <  2:1485676/150‑1 (MQ=255)
cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc                                                                                                                                                       <  1:968610/150‑1 (MQ=255)
cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc                                                                                                                                                       <  2:1652416/150‑1 (MQ=255)
cGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGAGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCCGCAGCCTGCTGcc                                                                                                                                                       >  2:1552556/1‑150 (MQ=255)
cGAAATGGAAATGAATTAAGCTTAAATGGTCGAGTATCGCTAAGAGCTGGTTAGCCACTTTTCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATGAGCACATGGCAAAAATCTCCAGCAGCCTGCTGcc                                                                                                                                                       <  1:331021/150‑1 (MQ=255)
 gAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCGCGCCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                                      >  2:1071557/1‑150 (MQ=255)
 gAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCg                                                                                                                                                      <  1:1526896/150‑1 (MQ=255)
  aaCTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCgg                                                                                                                                                     <  1:379937/150‑1 (MQ=255)
    cTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGGTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGag                                                                                                                                                   <  1:876706/150‑2 (MQ=255)
     tGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAAc                                                                                                                                                  <  1:1570509/150‑1 (MQ=255)
     tGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCAGGCTGCTGGagc                                                                                                                                                  <  1:189013/150‑3 (MQ=255)
       gAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTg                                                                                                                                                <  1:1335367/150‑1 (MQ=255)
          aaGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTct                                                                                                                                             >  2:1158271/1‑150 (MQ=255)
            gAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTctgc                                                                                                                                           >  2:537134/1‑150 (MQ=255)
            caaTAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATCCCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCGGCAGCAGGCTGCTGGAGCTTTttgc                                                                                                                                           <  1:1416566/149‑1 (MQ=255)
                 aaGCTGAACTAGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAg                                                                                                                                      >  1:1531243/1‑150 (MQ=255)
                    cTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaa                                                                                                                                   <  2:845865/150‑1 (MQ=255)
                     tGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                                  >  1:31576/1‑150 (MQ=255)
                     tGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCaaa                                                                                                                                  >  1:1135569/1‑150 (MQ=255)
                      gAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAAc                                                                                                                                 >  1:929987/1‑150 (MQ=255)
                       aaCTAGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAAcc                                                                                                                                <  2:1531243/150‑1 (MQ=255)
                           ggACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                                            <  1:823075/150‑1 (MQ=255)
                           ggACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGtt                                                                                                                            >  2:472590/1‑150 (MQ=255)
                             aCGAGTAGCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTcc                                                                                                                          <  2:14519/150‑1 (MQ=255)
                                   aTCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATc                                                                                                                    >  2:777730/1‑150 (MQ=255)
                                    tCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCg                                                                                                                   >  2:791973/1‑150 (MQ=255)
                                       cgAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCt                                                                                                                >  1:1597363/1‑150 (MQ=255)
                                        gAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTg                                                                                                               >  1:1250658/1‑150 (MQ=255)
                                             gCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCACACCCGTTCCGTAATCGTCTGGCaga                                                                                                          >  2:333222/1‑150 (MQ=255)
                                              cTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGCCTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCagag                                                                                                         >  2:833936/1‑150 (MQ=255)
                                              cTGGTTAGACACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCagag                                                                                                         >  1:251470/1‑150 (MQ=255)
                                               tGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGt                                                                                                        <  1:1307091/150‑1 (MQ=255)
                                                ggTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTGCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGtg                                                                                                       >  2:1390397/1‑149 (MQ=255)
                                                ggTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTc                                                                                                       <  2:811295/150‑1 (MQ=255)
                                                      ccACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGc                                                                                                 <  1:664485/150‑1 (MQ=255)
                                                       cACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                                                <  2:469236/150‑1 (MQ=255)
                                                       cACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                                                >  1:552296/1‑150 (MQ=255)
                                                       cACTTTGCCCGCAGCGCGGAATTACTGGAGACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGcc                                                                                                <  1:1710057/150‑1 (MQ=255)
                                                         cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCCCGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAg                                                                                              <  2:1426326/150‑1 (MQ=255)
                                                         cTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAg                                                                                              <  1:89139/150‑1 (MQ=255)
                                                                  cAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCa                                                                                     <  1:48512/150‑1 (MQ=255)
                                                                    gcgcTGAATTACTGGATACCATGGCGCACGACTATCGCGAGCTGTATCAGCACATGTCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAAGCCGTTCCGTAAGCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAgg                                                                                   <  1:1220643/150‑1 (MQ=255)
                                                                    gcgcGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAgg                                                                                   >  2:1658205/1‑150 (MQ=255)
                                                                     cgcgGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGc                                                                                  <  1:310950/150‑1 (MQ=255)
                                                                     cgcgGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGc                                                                                  <  1:994739/150‑1 (MQ=255)
                                                                        ggAATTACTGGATACCATGGCGCGCGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCAcc                                                                               <  1:791056/150‑1 (MQ=255)
                                                                           aTTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGt                                                                            >  1:1659485/1‑150 (MQ=255)
                                                                            ttACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTg                                                                           <  1:1593515/150‑1 (MQ=255)
                                                                            ttACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTg                                                                           <  2:875915/150‑1 (MQ=255)
                                                                            ttACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTg                                                                           <  1:258195/150‑1 (MQ=255)
                                                                             tACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGc                                                                          <  1:1556197/150‑1 (MQ=255)
                                                                             tACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGc                                                                          <  2:1135569/150‑1 (MQ=255)
                                                                              aCTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCa                                                                         >  2:979333/1‑150 (MQ=255)
                                                                                    tACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGcc                                                                   <  2:1597363/150‑1 (MQ=255)
                                                                                     aCCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCt                                                                  <  1:488085/150‑1 (MQ=255)
                                                                                      ccATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTc                                                                 >  1:559752/1‑150 (MQ=255)
                                                                                      ccATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTc                                                                 >  1:552965/1‑150 (MQ=255)
                                                                                            cgcACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCCGCAGCCTGCTGCCGGACCTGTCTGCTGCAGCAACCCCGTGCCGTCATCGTCTGGCAGCGTCTGAAGCCAGCAACGATAAGGCACCGGTGCAGATGCCTCGCGACt                                                           >  1:923295/1‑150 (MQ=255)
                                                                                            cgcACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACt                                                           >  2:738316/1‑150 (MQ=255)
                                                                                                cGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTc                                                       <  1:666740/150‑1 (MQ=255)
                                                                                                cGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGCAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTc                                                       >  2:1693952/1‑150 (MQ=255)
                                                                                                 gACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCt                                                      <  2:221520/150‑1 (MQ=255)
                                                                                                       cGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgc                                                <  1:778187/150‑1 (MQ=255)
                                                                                                        gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                               <  2:1255193/150‑1 (MQ=255)
                                                                                                        gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                               >  1:623230/1‑150 (MQ=255)
                                                                                                        gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                               >  2:1672008/1‑150 (MQ=255)
                                                                                                        gCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAACCCAGCAACGATCAGGCCCCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                               >  1:1648402/1‑150 (MQ=255)
                                                                                                        cccAGCTGTATCAGCACATGCCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGACGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcg                                               <  2:41189/149‑1 (MQ=255)
                                                                                                         ccAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGgcgc                                              >  2:1484061/1‑150 (MQ=255)
                                                                                                          cAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCa                                             <  1:340165/150‑1 (MQ=255)
                                                                                                            gCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATc                                           >  1:358644/1‑150 (MQ=255)
                                                                                                                    aGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgc                                   <  2:1659485/150‑1 (MQ=255)
                                                                                                                    aGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgc                                   >  1:1021677/1‑150 (MQ=255)
                                                                                                                    aGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGAAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgc                                   <  2:251470/150‑1 (MQ=255)
                                                                                                                      cacaTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCctgctg                                 >  1:765250/1‑150 (MQ=255)
                                                                                                                        caTGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCg                               >  1:173519/1‑150 (MQ=255)
                                                                                                                          tGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTa                             <  1:738316/150‑1 (MQ=255)
                                                                                                                           ggCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                                       <  2:1600325/107‑1 (MQ=255)
                                                                                                                           ggCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGa                                                                       >  1:1600325/1‑107 (MQ=255)
                                                                                                                            gCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACt                           >  2:705471/1‑150 (MQ=255)
                                                                                                                             cAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTg                          >  2:1720034/1‑150 (MQ=255)
                                                                                                                             cAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTg                          >  2:1665860/1‑150 (MQ=255)
                                                                                                                              aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCGGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTgg                         <  2:1175254/150‑1 (MQ=255)
                                                                                                                              aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTgg                         <  2:552965/150‑1 (MQ=255)
                                                                                                                              aaaaaGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTgg                         <  2:559752/150‑1 (MQ=255)
                                                                                                                               gacagttCCGGCAGCAGGCTGCTGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTAGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGgc                        >  1:1360458/7‑150 (MQ=255)
                                                                                                                                   gttCCGGCAGCAGGCTGCTGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGa                    >  1:1244853/3‑150 (MQ=255)
                                                                                                                                    cTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGaa                   >  1:1225561/1‑150 (MQ=255)
                                                                                                                                         gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGcgcg              >  2:1546907/1‑150 (MQ=255)
                                                                                                                                         gcagcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGcgcg              >  2:997444/1‑150 (MQ=255)
                                                                                                                                           agcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGAc            <  2:31576/150‑1 (MQ=255)
                                                                                                                                            gcCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGACCTGCTGCGTACTGGCGCGAAGCGCGACt           >  1:1348144/1‑150 (MQ=255)
                                                                                                                                                   gcCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAatttat    >  2:598077/1‑150 (MQ=255)
                                                                                                                                                     cGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAatttattt  >  1:1515469/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAACTGTCTGCTGAAGCAAACCCGTTCCGTAATCGTCTGGCAGAGTCTGAAGCCAGCAACGATCAGGCACCGGTGCAGATGCCTCGCGACTATTCTGAAGGCGCATCCGGCCTGCTGCGTACTGGCGCGAAGCGCGACTAATTTATTT  >  NC_000913/3380298‑3380596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: