Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,397,967 C→T 5.6% V138I (GTC→ATC) ‡ yhdE ← dTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,397,9670CT 5.6% 402.4 / 6.5 125V138I (GTC→ATC) ‡yhdEdTTP/UTP pyrophosphatase; m(5)UTP/m(5)CTP/pseudo‑UTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base C (56/61);  new base T (4/3);  total (61/64)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 126 total aligned reads displayed.

TGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCA  >  NC_000913/3397818‑3398115
                                                                                                                                                     |                                                                                                                                                    
tGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGAc                                                                                                                                                      >  2:418329/1‑150 (MQ=255)
  cGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACAt                                                                                                                                                    >  1:353685/1‑150 (MQ=255)
    ttAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCGCCagag                                                                                                                                                  >  2:1566636/1‑150 (MQ=255)
      aaaaTTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACCGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGcc                                                                                                                                                >  2:1460008/1‑150 (MQ=255)
       aaaTTACTTAATAAATCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGccc                                                                                                                                               <  2:1311970/150‑1 (MQ=255)
        aaTAACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGAATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcg                                                                                                                                              <  2:1195975/150‑1 (MQ=255)
          ttACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCcgcg                                                                                                                                            <  2:1427633/150‑1 (MQ=255)
            aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGAt                                                                                                                                          <  2:1218658/150‑1 (MQ=255)
            aCTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGATGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAt                                                                                                                                          >  1:925073/1‑150 (MQ=255)
             attAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATg                                                                                                                                         <  1:479276/149‑1 (MQ=255)
             attAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATg                                                                                                                                         <  1:1586601/149‑1 (MQ=255)
              ttAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGt                                                                                                                                        <  2:1328760/150‑1 (MQ=255)
               taataaTTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTc                                                                                                                                       >  1:148292/1‑150 (MQ=255)
                aataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCGGGCTATGTCGCCAGCGATgaac                                                                                                                                      <  1:1376971/150‑4 (MQ=255)
                 ataatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACACAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCtt                                                                                                                                     <  1:185634/150‑1 (MQ=255)
                  taatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATCGCGGGCTATGTcgcc                                                                                                                                    <  1:755520/150‑4 (MQ=255)
                  taatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATCGCGGGCTATGTcgcc                                                                                                                                    <  1:609936/150‑4 (MQ=255)
                  taatTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTc                                                                                                                                    >  1:1616256/1‑150 (MQ=255)
                     ttCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTc                                                                                                                                 <  2:1000088/150‑1 (MQ=255)
                          aCGTTTCCACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCGGCCAACACAGCCACCCAGCCCCTGAATACCGGATGCAGCTGCGTTATCTATCGGTTCATCGGTGGCGCCATCGCCCGGGATGTCGTCGTCTGttg                                                                                                                            >  2:1126162/1‑149 (MQ=255)
                                cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTc                                                                                                                      <  1:1001675/150‑1 (MQ=255)
                                cAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTc                                                                                                                      <  2:483511/150‑1 (MQ=255)
                                 aaCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTaa                                                                                                                           <  2:1229371/144‑1 (MQ=255)
                                 aaCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTaa                                                                                                                           >  1:1229371/1‑144 (MQ=255)
                                 aaCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCt                                                                                                                     <  1:332255/150‑1 (MQ=255)
                                 aaCCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCt                                                                                                                     <  2:216572/150‑1 (MQ=255)
                                  accaccGGTAAGCCGACTACGGCGTGATACCTGCAATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACATGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGAGATGTCTTCGTCTCTTAACGttata                                                                                                                    >  2:815070/1‑147 (MQ=255)
                                    cAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGaa                                                                                                                  <  1:138012/150‑1 (MQ=255)
                                     aGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGaaa                                                                                                                 >  2:1054259/1‑150 (MQ=255)
                                        ggTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTc                                                                                                              <  1:1157718/150‑1 (MQ=255)
                                             gCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATc                                                                                                         >  1:1466782/1‑150 (MQ=255)
                                                   tACGGAGTGATAGCTGCCATATATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGac                                                                                                   <  2:1584989/150‑1 (MQ=255)
                                                    aCGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGacc                                                                                                  >  1:63551/1‑150 (MQ=255)
                                                    aCGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAAAACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGATAACGTGCGGAAAGTCACATCGGTGccc                                                                                                  >  2:97711/1‑150 (MQ=255)
                                                     cGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGacca                                                                                                 >  1:521759/1‑150 (MQ=255)
                                                       gCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGaccacc                                                                                               >  2:54508/1‑150 (MQ=255)
                                                          tGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAgg                                                                                            <  1:694482/150‑1 (MQ=255)
                                                            aTAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCa                                                                                          >  1:1000364/1‑150 (MQ=255)
                                                            aTAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCa                                                                                          <  2:804762/150‑1 (MQ=255)
                                                               gCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATc                                                                                       <  2:353685/150‑1 (MQ=255)
                                                                cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCCCCAGGCAATCg                                                                                      >  1:1629581/1‑150 (MQ=255)
                                                                cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                      <  2:1310624/150‑1 (MQ=255)
                                                                cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                      <  2:1243752/150‑1 (MQ=255)
                                                                cTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCg                                                                                      <  1:504228/150‑1 (MQ=255)
                                                                  gCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCgag                                                                                    <  1:120207/150‑1 (MQ=255)
                                                                   ccATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCgaga                                                                                   <  1:1566270/150‑1 (MQ=255)
                                                                   ccATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCgaga                                                                                   >  1:956776/1‑150 (MQ=255)
                                                                   ccATTTATCTTCCTGACAAAACAGCCACCCAGCCCCGGAATACCGTATGCACCTGCTTTAGCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCgaga                                                                                   <  1:97711/150‑1 (MQ=255)
                                                                    cATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGaa                                                                                  <  1:1206295/150‑1 (MQ=255)
                                                                          tCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgct                                                                            <  1:133619/150‑1 (MQ=255)
                                                                           cTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctg                                                                           <  1:1453724/150‑1 (MQ=255)
                                                                            ttCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctgg                                                                          >  2:465466/1‑150 (MQ=255)
                                                                            ttCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctgg                                                                          <  2:148292/150‑1 (MQ=255)
                                                                             tCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCTTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctggc                                                                         >  2:677686/1‑150 (MQ=255)
                                                                              ccTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctggct                                                                        >  2:646388/1‑150 (MQ=255)
                                                                               cTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                              <  1:394002/143‑1 (MQ=255)
                                                                               cTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAAtgtg                                                                              >  2:394002/1‑143 (MQ=255)
                                                                               cTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGATCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTgctggctg                                                                       <  1:1255610/150‑1 (MQ=255)
                                                                                   cAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCgg                                                                   >  2:1544157/1‑150 (MQ=255)
                                                                                    aaaaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGc                                                                  <  2:1487223/150‑1 (MQ=255)
                                                                                    aaaaCAGCCACCCAGCCCCTGAATACAGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCTGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGc                                                                  <  1:1224415/150‑1 (MQ=255)
                                                                                     aaaCAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGCCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCAATCGAGAATGTGCTGGCTGTCGGcc                                                                 >  2:594130/1‑150 (MQ=255)
                                                                                       aCAGCCACCCAGCCCCTGAATACCGTATGCACATGCTTTATCTAACGGTTCATAGCTGGCGACATAGCAAGCGATGTCTTCGACTGTTAACGTTCTGAAAGTAACATCAGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCaa                                                               >  2:1700127/1‑150 (MQ=255)
                                                                                          gCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGc                                                            <  2:521759/150‑1 (MQ=255)
                                                                                           ccACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGcc                                                           <  1:674597/150‑1 (MQ=255)
                                                                                            cACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCa                                                          <  2:907175/150‑1 (MQ=255)
                                                                                             aCCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCAc                                                         >  1:813452/1‑150 (MQ=255)
                                                                                                cAGCCCCTGAATACCGTATGCACCTGCTTTGTCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGAACACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCACAGCCActgc                                                      >  1:206025/1‑150 (MQ=255)
                                                                                                 aGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCActgct                                                     <  1:1350995/150‑1 (MQ=255)
                                                                                                  gCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCActgctg                                                    <  1:54508/150‑1 (MQ=255)
                                                                                                   ccccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGt                                                   >  1:690720/1‑150 (MQ=255)
                                                                                                    cccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGAGGTCTTCGTCGGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtg                                                  >  2:94269/1‑149 (MQ=255)
                                                                                                     ccTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtca                                                 >  2:1163031/1‑150 (MQ=255)
                                                                                                       tGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTGACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtcatc                                               <  2:1117487/150‑1 (MQ=255)
                                                                                                       tGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtcatc                                               <  1:1519262/150‑1 (MQ=255)
                                                                                                       tGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtcatc                                               <  1:631346/150‑1 (MQ=255)
                                                                                                       tGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGtcatc                                               <  1:741928/150‑1 (MQ=255)
                                                                                                          aTACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCAcc                                            >  1:420885/1‑150 (MQ=255)
                                                                                                           tACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCCACGCCACTGCTGTCATCACCt                                           >  2:843028/1‑150 (MQ=255)
                                                                                                            aCCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTg                                          <  1:1434898/150‑1 (MQ=255)
                                                                                                             ccGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGa                                         >  2:1160517/1‑150 (MQ=255)
                                                                                                                tATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATgg                                      <  2:1224195/150‑1 (MQ=255)
                                                                                                                 aTGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATggg                                     <  1:440169/150‑1 (MQ=255)
                                                                                                                 aTGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATggg                                     <  1:1544157/150‑1 (MQ=255)
                                                                                                                  tGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGt                                    >  2:1514227/1‑150 (MQ=255)
                                                                                                                      ccTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGa                                >  1:1309507/1‑150 (MQ=255)
                                                                                                                       cTGCTTTATCTAACGGTGCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAc                               <  2:956776/150‑1 (MQ=255)
                                                                                                                         gCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGAccc                             <  1:1163031/150‑1 (MQ=255)
                                                                                                                            ttATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGCCCCGAt                          >  2:1627548/1‑150 (MQ=255)
                                                                                                                             tATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATa                         >  1:364452/1‑150 (MQ=255)
                                                                                                                              aTCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATaa                        >  2:119803/1‑150 (MQ=255)
                                                                                                                                  aaCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCTTGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTg                    <  1:1700127/150‑1 (MQ=255)
                                                                                                                                  aaCGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTg                    <  2:323611/150‑1 (MQ=255)
                                                                                                                                     ggTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTgcgc                 <  2:690720/150‑1 (MQ=255)
                                                                                                                                      gTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCa                >  1:705898/1‑150 (MQ=255)
                                                                                                                                        acATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAAc              >  2:1125716/2‑150 (MQ=255)
                                                                                                                                         cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGCCCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa             >  1:551116/1‑150 (MQ=255)
                                                                                                                                         cATCGCGGGCTATGTCGCCAGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACa             >  1:690595/1‑150 (MQ=255)
                                                                                                                                              cTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTgc        >  1:477859/1‑150 (MQ=255)
                                                                                                                                                    aCATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATAGCCTGATGGGTCTGACCGGTGAATTTGCGCAGCATCTgcgcgggg  >  2:1630066/1‑146 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCGTTAAAATTACTTAATAATTCATACGTTTCAACCAGCGGTAAGCCGACTACGGCGTGATAGCTGCCATTTATCTTCCTGACAAAACAGCCACCCAGCCCCTGAATACCGTATGCACCTGCTTTATCTAACGGTTCATCGCTGGCGACATAGCCCGCGATGTCTTCGTCTGTTAACGTTCTGAAAGTCACATCGGTGACCACCAGGCAATCGAGAATGTGCTGGCTGTCGGCCAACGCCACTGCTGTCATCACCTGATGGGTCTGACCCGATAATTTGCGCAACATCTGCGCCGCA  >  NC_000913/3397818‑3398115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: