Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,405,885 G→C 6.7% E150D (GAG→GAC accB → acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,405,8850GC 6.7% 432.3 / 17.4 134E150D (GAG→GACaccBacetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base G (59/65);  new base C (5/4);  total (65/69)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

Only 100 of 137 total aligned reads displayed.

GCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATC  >  NC_000913/3405736‑3406031
                                                                                                                                                     |                                                                                                                                                  
gCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAg                                                                                                                                                    <  2:12019/150‑1 (MQ=255)
gCGTTCATCGAAGGGCGTCAGAAAGTCAACGTGGGCGCTACCCTGTGCCTCGTTGCAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAg                                                                                                                                                    <  2:1539024/150‑1 (MQ=255)
 cGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAGGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGc                                                                                                                                                   >  2:512115/1‑150 (MQ=255)
 cGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGAACGAAGCGGACAAATCAGGTACCTAGAAAGCAATTATGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGc                                                                                                                                                   >  2:837225/1‑150 (MQ=255)
  gTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGcc                                                                                                                                                  >  1:1271686/1‑150 (MQ=255)
  gTTCAGCGCAGTCGGTCAGACAGTCAACGTGGTCGATACCCAGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGcc                                                                                                                                                  <  2:232342/150‑1 (MQ=255)
    tCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGc                                                                                                                                                >  1:273649/1‑150 (MQ=255)
       tCGAAGTGGGTCAGAAAGTCAACCTGGGCGATACCCTGTGCATAGTTGAACCCATGAAAAAGATGAACCAGATCGAAGCGGACAAATACGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGAAAACCCGTAGAATTTGACGATCCGCTgg                                                                                                                                             >  2:536231/1‑150 (MQ=255)
        cGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCAATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgt                                                                                                                                            <  2:141446/150‑1 (MQ=255)
         gAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                                                                                                                           >  1:595706/1‑150 (MQ=255)
         gAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                                                                                                                           >  2:756062/1‑150 (MQ=255)
          aaGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcg                                                                                                                                          >  2:1192460/1‑150 (MQ=255)
              tggTCTGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAACCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaa                                                                                                                                      <  1:1317163/149‑2 (MQ=255)
              gggTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACACCCGGTAGAATTTGACGAGCCGCTGGTCGTCAt                                                                                                                                      >  2:437263/1‑150 (MQ=255)
               ggTCAGAAAGTCAACGTGGGCGAAACCCGGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaaa                                                                                                                                     <  1:624855/150‑3 (MQ=255)
                gTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                    <  2:1485697/150‑1 (MQ=255)
                gTCACAAAGTCAACGTGGGCGAAACCCTGAGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaaat                                                                                                                                    <  1:374805/150‑4 (MQ=255)
                         tCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAg                                                                                                                           >  2:1175135/1‑150 (MQ=255)
                          cAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAgg                                                                                                                          >  1:1680802/1‑150 (MQ=255)
                            aCGTGGGCGATAAACTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGTACAACAGGTAGAATTTGACTAGCCGCTTGTCGTCATCGAGTAACGAGGcc                                                                                                                        >  1:820103/1‑149 (MQ=255)
                              gTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGaa                                                                                                                      <  2:1617988/150‑1 (MQ=255)
                                gggCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACa                                                                                                                    <  1:631752/150‑1 (MQ=255)
                                gggCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACa                                                                                                                    >  2:1131396/1‑150 (MQ=255)
                                   cGATACCCTGTTCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGc                                                                                                                 <  2:487807/150‑1 (MQ=255)
                                    gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCt                                                                                                                <  1:246432/150‑1 (MQ=255)
                                    gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCt                                                                                                                <  2:1660782/150‑1 (MQ=255)
                                     atccccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg                                                                                                               <  1:1537194/147‑1 (MQ=255)
                                     aTACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg                                                                                                               <  2:1251512/150‑1 (MQ=255)
                                      tACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTgg                                                                                                              >  1:935353/1‑150 (MQ=255)
                                        cccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTTACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGAt                                                                                                            >  1:1394103/1‑150 (MQ=255)
                                        cccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGACGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGAt                                                                                                            >  1:1555429/1‑150 (MQ=255)
                                           tgtgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCCTCGAGTAACGAGGCGAACATGCTGGATaaa                                                                                                         >  2:1311668/1‑150 (MQ=255)
                                             ggCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAAt                                                                                                       <  2:369277/149‑1 (MQ=255)
                                               cATCTTTTAAGCCATTATAATGATGAACCAGATCGAAGCTGACAAATCCGGTACCGTGAAAGAAATTCTGGTCGAAAGTCGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                     <  2:356224/150‑1 (MQ=255)
                                               cATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                     >  1:1376215/1‑150 (MQ=255)
                                               cATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                     <  1:1175135/150‑1 (MQ=255)
                                               cATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                     >  2:1162839/1‑150 (MQ=255)
                                                  cGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTa                                                                                                  >  1:786766/1‑150 (MQ=255)
                                                     tGAAGCCTTGATAATGATGATCCCGACCGAAGCGGACCAATCCGGTACCCTGAAAGCAATTCTGGGCGAAAGTGGACTACCGGAAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTg                                                                                               <  2:720104/150‑1 (MQ=255)
                                                      gAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGc                                                                                              <  2:1523266/150‑1 (MQ=255)
                                                        aGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACAAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCa                                                                                            <  2:772946/150‑1 (MQ=255)
                                                         gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                    <  2:585584/109‑1 (MQ=255)
                                                         gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCaa                                                                                           >  1:119346/1‑150 (MQ=255)
                                                             tGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACcgc                                                                                       >  1:1497866/1‑150 (MQ=255)
                                                                aaaTGATGAACCAGATCGAAGCGGTCAAATCCGGTACCGTGAAAGCTATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCgcggc                                                                                    <  1:1404419/150‑1 (MQ=255)
                                                                aaaTGATGAACCAGATCGAAGCGGACAAATCCGGTACCGGGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGCGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCgcggc                                                                                    <  2:1255186/150‑1 (MQ=255)
                                                                 aaTGATGAACCAGATCGAAGCGGACAAATCCGGTACTGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCgcggcg                                                                                   <  1:676101/150‑1 (MQ=255)
                                                                  attatgtACCAGATCGAAGAGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACATCCGGTAGAATTTGAAGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCga                                                                                  <  1:542859/147‑1 (MQ=255)
                                                                  attatgaACCAGATCGAAGCGGACTATTCTGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACTAGCGGCTGTTCGTCATCGATTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCga                                                                                  <  1:536231/147‑1 (MQ=255)
                                                                   tgatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                    <  2:160824/99‑1 (MQ=255)
                                                                   tgatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                    >  1:160824/1‑99 (MQ=255)
                                                                        aaCCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgc                                                                            >  1:1000772/1‑150 (MQ=255)
                                                                         aCCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgca                                                                           >  1:1268561/1‑150 (MQ=255)
                                                                           cAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcatt                                                                         >  1:1575480/1‑150 (MQ=255)
                                                                            aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                        <  1:372313/150‑1 (MQ=255)
                                                                            aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                        >  2:198705/1‑150 (MQ=255)
                                                                             gATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattgc                                                                       >  2:713668/1‑150 (MQ=255)
                                                                              ctCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCg                                                                      <  2:922613/149‑1 (MQ=255)
                                                                               tCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGt                                                                     <  2:595706/150‑1 (MQ=255)
                                                                                caaaGCGGACAAATCAGGTACCGAGAAAGAAATTCAGGTCGAAAGTGGACAACCGGTAGAATTTGACTAGCCGCTGGTCGTCATCGAGTAACGAGACGAACATGATGGAAAAAATTGTTATATCAAACAACGGCGAGATTGCATTGCCTa                                                                    >  2:381806/3‑150 (MQ=255)
                                                                                cGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTa                                                                    <  2:1680802/150‑1 (MQ=255)
                                                                                  aaGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGGCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAtt                                                                  <  2:29629/150‑1 (MQ=255)
                                                                                   aGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttc                                                                 <  2:1497866/150‑1 (MQ=255)
                                                                                      ggACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttcttc                                                              <  2:119346/150‑1 (MQ=255)
                                                                                       gACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCg                                                             <  1:1192460/150‑1 (MQ=255)
                                                                                           aaTCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGcc                                                         <  2:935353/150‑1 (MQ=255)
                                                                                                ggTACCGTGAAAGCAATTCTGGGCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTaa                                                    <  2:307631/150‑1 (MQ=255)
                                                                                                  tACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAg                                                  >  1:1383122/1‑150 (MQ=255)
                                                                                                   aCCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGa                                                 <  2:158024/150‑1 (MQ=255)
                                                                                                      gTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACt                                              >  2:1261550/1‑150 (MQ=255)
                                                                                                      gTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGAATTCTTCGTGCCTGTAAAGAACt                                              >  1:1682713/1‑150 (MQ=255)
                                                                                                       tGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTg                                             >  1:178777/1‑150 (MQ=255)
                                                                                                        gAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATACTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTgg                                            >  1:387614/1‑150 (MQ=255)
                                                                                                         aaaGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTggg                                           <  2:1000772/150‑1 (MQ=255)
                                                                                                              aaTTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCa                                      <  1:713668/150‑1 (MQ=255)
                                                                                                                ttCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAg                                    <  2:786766/150‑1 (MQ=255)
                                                                                                                 tCTGTTCGTAATTTGACTACCGGTATATTTTGACGAGTCGCTGGTCTTCATCGAGTAACGAGGACAACATGCAGGATAAAATTGTTATTGCCAACAGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGa                                   <  1:837225/150‑1 (MQ=255)
                                                                                                                 tCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGa                                   <  1:512115/150‑1 (MQ=255)
                                                                                                                  cTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGAc                                  <  2:1167992/150‑1 (MQ=255)
                                                                                                                  cTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGAc                                  >  1:516110/1‑150 (MQ=255)
                                                                                                                    ggTCGAAAGTCGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTg                                <  2:1394103/150‑1 (MQ=255)
                                                                                                                     gTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGt                               >  1:1650062/1‑150 (MQ=255)
                                                                                                                         aaaGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCt                           >  2:810572/1‑150 (MQ=255)
                                                                                                                            gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGGCTGTCGCtgtg                        <  1:352734/150‑1 (MQ=255)
                                                                                                                            gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                        <  2:427573/150‑1 (MQ=255)
                                                                                                                            gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                        >  2:1105100/1‑150 (MQ=255)
                                                                                                                            gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                        <  2:1282964/150‑1 (MQ=255)
                                                                                                                               gACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                     <  2:5595/150‑1 (MQ=255)
                                                                                                                               gACAACCGGTAGAAGTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                     <  1:1681193/150‑1 (MQ=255)
                                                                                                                                     cGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgc               >  2:814411/1‑150 (MQ=255)
                                                                                                                                      ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg              <  2:1396022/150‑1 (MQ=255)
                                                                                                                                      ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg              >  2:473742/1‑150 (MQ=255)
                                                                                                                                         aGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCgg           <  1:1105100/150‑1 (MQ=255)
                                                                                                                                          ctcgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGCCTGTCGCTGTGCACTCCAGCGCGGa          >  2:218492/6‑150 (MQ=255)
                                                                                                                                          ctcgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGa          >  2:1372301/6‑150 (MQ=255)
                                                                                                                                           aaTTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATACTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGAt         <  2:387614/150‑1 (MQ=255)
                                                                                                                                            cgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTCTTCTTCGTGCCTGTAAAGCACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATc        >  1:1049629/4‑150 (MQ=255)
                                                                                                                                               tGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGAGCACTCCAGCGCGGATcgcg     >  2:468119/1‑150 (MQ=255)
                                                                                                                                               tGACGAGCCGCTGGTCGTCATAGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATcgcg     >  1:971328/1‑150 (MQ=255)
                                                                                                                                                  cGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATc  <  2:273649/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCGTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATC  >  NC_000913/3405736‑3406031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: