Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,405,887 C→A 7.0% P151Q (CCG→CAG)  accB → acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,405,8870CA 7.0% 383.5 / 15.2 128P151Q (CCG→CAG) accBacetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base C (56/62);  new base A (5/4);  total (61/67)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.93e-01

Only 100 of 132 total aligned reads displayed.

GTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAA  >  NC_000913/3405738‑3406035
                                                                                                                                                     |                                                                                                                                                    
gTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGcc                                                                                                                                                      <  2:445625/150‑1 (MQ=255)
gTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGcc                                                                                                                                                      >  1:1271686/1‑150 (MQ=255)
gTTCAGCGCAGTCGGTCAGACAGTCAACGTGGTCGATACCCAGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGcc                                                                                                                                                      <  2:232342/150‑1 (MQ=255)
  tCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGc                                                                                                                                                    >  1:273649/1‑150 (MQ=255)
     tCGAAGTGGGTCATAAAGTCAACGTGGGCGATACCCTGTGCATAGTTGAAGCCATTAAAAAGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGACGCTgg                                                                                                                                                 >  2:542859/1‑150 (MQ=255)
     tCGAAGTGGGTCAGAAAGTCAACCTGGGCGATACCCTGTGCATAGTTGAACCCATGAAAAAGATGAACCAGATCGAAGCGGACAAATACGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGAAAACCCGTAGAATTTGACGATCCGCTgg                                                                                                                                                 >  2:536231/1‑150 (MQ=255)
      cGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCAATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgt                                                                                                                                                <  2:141446/150‑1 (MQ=255)
       gAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                                                                                                                               >  1:595706/1‑150 (MQ=255)
       gAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                                                                                                                               >  2:756062/1‑150 (MQ=255)
        aaGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtcg                                                                                                                                              >  2:1192460/1‑150 (MQ=255)
            tggTCTGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAACCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaa                                                                                                                                          <  1:1317163/149‑2 (MQ=255)
            gggTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaa                                                                                                                                          <  2:1013591/150‑2 (MQ=255)
              gTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                        <  2:1485697/150‑1 (MQ=255)
              gTCAGAAAGGCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                        <  2:724551/150‑1 (MQ=255)
              gTCACAAAGTCAACGTGGGCGAAACCCTGAGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaaat                                                                                                                                        <  1:374805/150‑4 (MQ=255)
               tCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGa                                                                                                                                       >  1:158024/1‑150 (MQ=255)
                    aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAAc                                                                                                                                  >  2:1355285/1‑150 (MQ=255)
                     aGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACg                                                                                                                                 <  2:792118/150‑1 (MQ=255)
                      gTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTa                                                                                                                                    >  1:383315/1‑146 (MQ=255)
                      gTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTa                                                                                                                                    <  2:383315/146‑1 (MQ=255)
                        cAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAgg                                                                                                                              >  1:1680802/1‑150 (MQ=255)
                          aCGTGGGCGATAAACTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGTACAACAGGTAGAATTTGACTAGCCGCTTGTCGTCATCGAGTAACGAGGcc                                                                                                                            >  1:820103/1‑149 (MQ=255)
                            gTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGaa                                                                                                                          <  2:1617988/150‑1 (MQ=255)
                            gTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGaa                                                                                                                          >  2:1404419/1‑150 (MQ=255)
                              gggCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACa                                                                                                                        <  1:631752/150‑1 (MQ=255)
                                 cGATACCCTGTTCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGc                                                                                                                     <  2:487807/150‑1 (MQ=255)
                                  gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCt                                                                                                                    <  1:246432/150‑1 (MQ=255)
                                   atccccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg                                                                                                                   <  1:1537194/147‑1 (MQ=255)
                                   aTACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg                                                                                                                   <  2:1251512/150‑1 (MQ=255)
                                    tACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTgg                                                                                                                  >  1:935353/1‑150 (MQ=255)
                                      cccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTTACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGAt                                                                                                                >  1:1394103/1‑150 (MQ=255)
                                           tgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAAt                                                                                                           <  1:756062/150‑1 (MQ=255)
                                           ggCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAAt                                                                                                           <  2:369277/149‑1 (MQ=255)
                                             cATCTTTTAAGCCATTATAATGATGAACCAGATCGAAGCTGACAAATCCGGTACCGTGAAAGAAATTCTGGTCGAAAGTCGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                         <  2:356224/150‑1 (MQ=255)
                                             cATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                         >  2:1162839/1‑150 (MQ=255)
                                                cGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTa                                                                                                      >  1:786766/1‑150 (MQ=255)
                                                 gTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTAt                                                                                                     >  2:352734/1‑150 (MQ=255)
                                                  ttGAAGCCATGAAAATGATGAACCAGAGCGAAGCGGACAAATCCGGTACCGTGAAACCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTAtt                                                                                                    <  2:162497/150‑1 (MQ=255)
                                                   tGAAGCCTTGATAATGATGATCCCGACCGAAGCGGACCAATCCGGTACCCTGAAAGCAATTCTGGGCGAAAGTGGACTACCGGAAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTg                                                                                                   <  2:720104/150‑1 (MQ=255)
                                                    gAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGc                                                                                                  <  2:1523266/150‑1 (MQ=255)
                                                     aaGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGcc                                                                                                 >  1:648317/1‑150 (MQ=255)
                                                       gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                        >  1:585584/1‑109 (MQ=255)
                                                       gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                        <  2:585584/109‑1 (MQ=255)
                                                       gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCaa                                                                                               >  1:119346/1‑150 (MQ=255)
                                                        ccATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTTGATAAAATTGTTATTGCCAAc                                                                                              >  2:628217/1‑150 (MQ=255)
                                                           tGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACcgc                                                                                           >  1:1497866/1‑150 (MQ=255)
                                                               aaTGATGAACCAGATCGAAGCGGACAAATCCGGTACTGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCgcggcg                                                                                       <  1:676101/150‑1 (MQ=255)
                                                                attatgtACCAGATCGAAGAGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACATCCGGTAGAATTTGAAGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCga                                                                                      <  1:542859/147‑1 (MQ=255)
                                                                 tgatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                        <  2:160824/99‑1 (MQ=255)
                                                                 tgatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                        >  1:160824/1‑99 (MQ=255)
                                                                     gaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattg                                                                                 >  1:1167992/1‑150 (MQ=255)
                                                                     gaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattg                                                                                 >  2:211533/1‑150 (MQ=255)
                                                                      aaCCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgc                                                                                >  1:1000772/1‑150 (MQ=255)
                                                                         cAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcatt                                                                             >  1:1575480/1‑150 (MQ=255)
                                                                          aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                            >  2:198705/1‑150 (MQ=255)
                                                                          aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                            <  1:372313/150‑1 (MQ=255)
                                                                          aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATATGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                            <  1:1503469/150‑1 (MQ=255)
                                                                            ctCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCg                                                                          <  2:922613/149‑1 (MQ=255)
                                                                             tCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGt                                                                         <  2:595706/150‑1 (MQ=255)
                                                                              cGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTa                                                                        <  2:1680802/150‑1 (MQ=255)
                                                                                   cGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttctt                                                                   >  1:1389965/1‑150 (MQ=255)
                                                                                    ggACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttcttc                                                                  <  2:119346/150‑1 (MQ=255)
                                                                                     gACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCg                                                                 <  1:1192460/150‑1 (MQ=255)
                                                                                      aCAAATCCGTTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGt                                                                <  2:868333/150‑1 (MQ=255)
                                                                                       cAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTg                                                               <  1:1221995/150‑1 (MQ=255)
                                                                                         aaTCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGcc                                                             <  2:935353/150‑1 (MQ=255)
                                                                                             cGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCGAACCGCGGCGAGATTGCATTGCGTTTTCTTCGTGCCTGTa                                                         <  2:1185497/150‑1 (MQ=255)
                                                                                              ggTACCGTGAAAGCAATTCTGGGCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTaa                                                        <  2:307631/150‑1 (MQ=255)
                                                                                                tACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAg                                                      >  1:1383122/1‑150 (MQ=255)
                                                                                                    gTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACt                                                  >  2:1261550/1‑150 (MQ=255)
                                                                                                    gTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGAATTCTTCGTGCCTGTAAAGAACt                                                  >  1:1682713/1‑150 (MQ=255)
                                                                                                     tGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTg                                                 >  1:178777/1‑150 (MQ=255)
                                                                                                        aaGCAATTCTGGTCGAACGTGGACAACCGGTAGAATTTGACGAGCCGCGGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAATTTGTTAATGCCTACCGCGGCGAGATTGCATTGCGGATTCTTCGTGCCTGTAAAGAACTGGGc                                              <  1:844382/150‑1 (MQ=255)
                                                                                                              ttCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAg                                        <  2:786766/150‑1 (MQ=255)
                                                                                                               tCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGa                                       <  1:512115/150‑1 (MQ=255)
                                                                                                                cTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGAc                                      <  2:1167992/150‑1 (MQ=255)
                                                                                                                cTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGAc                                      >  1:516110/1‑150 (MQ=255)
                                                                                                                  ggTCGAAAGTCGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTg                                    <  2:1394103/150‑1 (MQ=255)
                                                                                                                       aaaGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCt                               >  2:810572/1‑150 (MQ=255)
                                                                                                                          gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGGCTGTCGCtgtg                            <  1:352734/150‑1 (MQ=255)
                                                                                                                          gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                            >  2:269501/1‑150 (MQ=255)
                                                                                                                          gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                            <  2:1282964/150‑1 (MQ=255)
                                                                                                                          gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                            >  2:1105100/1‑150 (MQ=255)
                                                                                                                          gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                            <  2:427573/150‑1 (MQ=255)
                                                                                                                             gACAACCGGTAGAAGTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                         <  1:1681193/150‑1 (MQ=255)
                                                                                                                             gACAACCGGTAGAAATTGACGAGCCGCTGGGCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                         <  2:1505809/150‑1 (MQ=255)
                                                                                                                                    ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg                  >  2:473742/1‑150 (MQ=255)
                                                                                                                                    ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg                  >  2:1548905/1‑150 (MQ=255)
                                                                                                                                       aGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCgg               <  1:513220/150‑1 (MQ=255)
                                                                                                                                       aGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCgg               <  1:1105100/150‑1 (MQ=255)
                                                                                                                                        ctcgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGa              >  2:1372301/6‑150 (MQ=255)
                                                                                                                                         tcgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGAt             >  1:1351574/5‑150 (MQ=255)
                                                                                                                                         aaTTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATACTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGAt             <  2:387614/150‑1 (MQ=255)
                                                                                                                                          cgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTCTTCTTCGTGCCTGTAAAGCACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATc            >  1:1049629/4‑150 (MQ=255)
                                                                                                                                           gatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGCCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTTTCGCTGTGCACTCCAGCGCGGATcg           >  2:1151807/3‑150 (MQ=255)
                                                                                                                                             tGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGAGCACTCCAGCGCGGATcgcg         >  2:468119/1‑150 (MQ=255)
                                                                                                                                             tGACGAGCCGCTGGTCGTCATAGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATcgcg         >  1:971328/1‑150 (MQ=255)
                                                                                                                                                cGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATc      <  2:273649/150‑1 (MQ=255)
                                                                                                                                                cGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATc      <  2:1268561/150‑1 (MQ=255)
                                                                                                                                                    ccGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTaaa  >  2:1050673/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTCATCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAA  >  NC_000913/3405738‑3406035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: