Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,405,892 G→C 7.1% V153L (GTC→CTC)  accB → acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,405,8920GC 7.1% 412.5 / 15.7 124V153L (GTC→CTC) accBacetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base G (51/63);  new base C (5/4);  total (56/68)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGT  >  NC_000913/3405743‑3406041
                                                                                                                                                     |                                                                                                                                                     
tCGAAGTGGGTCATAAAGTCAACGTGGGCGATACCCTGTGCATAGTTGAAGCCATTAAAAAGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGACGCTgg                                                                                                                                                       >  2:542859/1‑150 (MQ=255)
tCGAAGTGGGTCAGAAAGTCAACCTGGGCGATACCCTGTGCATAGTTGAACCCATGAAAAAGATGAACCAGATCGAAGCGGACAAATACGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGAAAACCCGTAGAATTTGACGATCCGCTgg                                                                                                                                                       >  2:536231/1‑150 (MQ=255)
  gAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                                                                                                                                     >  2:756062/1‑150 (MQ=255)
  gAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGgtc                                                                                                                                                     >  1:595706/1‑150 (MQ=255)
       tggTCTGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAACCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaa                                                                                                                                                <  1:1317163/149‑2 (MQ=255)
       gggTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACACCCGGTAGAATTTGACGAGCCGCTGGTCGTCAt                                                                                                                                                >  2:437263/1‑150 (MQ=255)
       gggTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaa                                                                                                                                                <  2:1013591/150‑2 (MQ=255)
        ggTCAGAAAGTCAACGTGGGCGAAACCCGGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaaa                                                                                                                                               <  1:624855/150‑3 (MQ=255)
         gTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                              <  2:1485697/150‑1 (MQ=255)
         gTCAGAAAGGCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                              <  2:724551/150‑1 (MQ=255)
         gTCACAAAGTCAACGTGGGCGAAACCCTGAGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGACCAGCGGCTCGTCaaat                                                                                                                                              <  1:374805/150‑4 (MQ=255)
          tCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGa                                                                                                                                             >  1:158024/1‑150 (MQ=255)
               aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACACCCGGTAGAATTTTACGCGCCGCTGGTCGTCATCGAGTAAc                                                                                                                                        >  1:301064/1‑150 (MQ=255)
               aaGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAAc                                                                                                                                        >  2:1355285/1‑150 (MQ=255)
                aGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACg                                                                                                                                       <  2:792118/150‑1 (MQ=255)
                 gTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTa                                                                                                                                          <  2:383315/146‑1 (MQ=255)
                 gTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTa                                                                                                                                          >  1:383315/1‑146 (MQ=255)
                  tCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAg                                                                                                                                     >  2:1175135/1‑150 (MQ=255)
                     aCGTGGGCGATAAACTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGTACAACAGGTAGAATTTGACTAGCCGCTTGTCGTCATCGAGTAACGAGGcc                                                                                                                                  >  1:820103/1‑149 (MQ=255)
                       gTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGaa                                                                                                                                >  2:1404419/1‑150 (MQ=255)
                         gggCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACa                                                                                                                              >  2:1131396/1‑150 (MQ=255)
                            cGATACCCTGTTCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGc                                                                                                                           <  2:487807/150‑1 (MQ=255)
                             gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCt                                                                                                                          <  2:1660782/150‑1 (MQ=255)
                             gATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCt                                                                                                                          <  1:246432/150‑1 (MQ=255)
                              atccccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg                                                                                                                         <  1:1537194/147‑1 (MQ=255)
                              aTACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTg                                                                                                                         <  2:1251512/150‑1 (MQ=255)
                               tACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTgg                                                                                                                        >  1:935353/1‑150 (MQ=255)
                                 cccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTTACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGAt                                                                                                                      >  1:1394103/1‑150 (MQ=255)
                                 cccTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGACGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGAt                                                                                                                      >  1:1555429/1‑150 (MQ=255)
                                    tgtgCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCCTCGAGTAACGAGGCGAACATGCTGGATaaa                                                                                                                   >  2:1311668/1‑150 (MQ=255)
                                        cATCTTTTAAGCCATTATAATGATGAACCAGATCGAAGCTGACAAATCCGGTACCGTGAAAGAAATTCTGGTCGAAAGTCGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTg                                                                                                               <  2:356224/150‑1 (MQ=255)
                                           cGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTa                                                                                                            >  1:786766/1‑150 (MQ=255)
                                            gTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTAt                                                                                                           >  2:352734/1‑150 (MQ=255)
                                             ttGAAGCCATGAAAATGATGAACCAGAGCGAAGCGGACAAATCCGGTACCGTGAAACCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTAtt                                                                                                          <  2:162497/150‑1 (MQ=255)
                                              tGAAGCCTTGATAATGATGATCCCGACCGAAGCGGACCAATCCGGTACCCTGAAAGCAATTCTGGGCGAAAGTGGACTACCGGAAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTg                                                                                                         <  2:720104/150‑1 (MQ=255)
                                                aaGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGcc                                                                                                       >  1:648317/1‑150 (MQ=255)
                                                  gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                              >  1:585584/1‑109 (MQ=255)
                                                  gCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCaa                                                                                                     >  1:119346/1‑150 (MQ=255)
                                                   ccATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTTGATAAAATTGTTATTGCCAAc                                                                                                    >  2:628217/1‑150 (MQ=255)
                                                      tGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACcgc                                                                                                 >  1:1497866/1‑150 (MQ=255)
                                                         aaaTGATGAACCAGATCGAAGCGGACAAATCCGGTACCGGGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGCGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCgcggc                                                                                              <  2:1255186/150‑1 (MQ=255)
                                                          aaTGATGAACCAGATCGAAGCGGACAAATCCGGTACTGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCgcggcg                                                                                             <  1:676101/150‑1 (MQ=255)
                                                           attatgaACCAGATCGAAGCGGACTATTCTGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACTAGCGGCTGTTCGTCATCGATTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCga                                                                                            <  1:536231/147‑1 (MQ=255)
                                                            tgatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                              <  2:160824/99‑1 (MQ=255)
                                                            tgatgaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCg                                                                                                                                              >  1:160824/1‑99 (MQ=255)
                                                                gaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattg                                                                                       >  1:1167992/1‑150 (MQ=255)
                                                                gaACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattg                                                                                       >  2:211533/1‑150 (MQ=255)
                                                                 aaCCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgc                                                                                      >  1:1000772/1‑150 (MQ=255)
                                                                     aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                                  <  1:372313/150‑1 (MQ=255)
                                                                     aGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATATGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGattgcattg                                                                                  <  1:1503469/150‑1 (MQ=255)
                                                                       ctCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCg                                                                                <  2:922613/149‑1 (MQ=255)
                                                                        tCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGt                                                                               <  2:595706/150‑1 (MQ=255)
                                                                         caaaGCGGACAAATCAGGTACCGAGAAAGAAATTCAGGTCGAAAGTGGACAACCGGTAGAATTTGACTAGCCGCTGGTCGTCATCGAGTAACGAGACGAACATGATGGAAAAAATTGTTATATCAAACAACGGCGAGATTGCATTGCCTa                                                                              >  2:381806/3‑150 (MQ=255)
                                                                         cGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTa                                                                              <  2:1680802/150‑1 (MQ=255)
                                                                            aGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttc                                                                           <  2:1497866/150‑1 (MQ=255)
                                                                              cGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttctt                                                                         >  1:1389965/1‑150 (MQ=255)
                                                                               ggACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTAttcttc                                                                        <  2:119346/150‑1 (MQ=255)
                                                                                gACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCg                                                                       <  1:1192460/150‑1 (MQ=255)
                                                                                 aCAAATCCGTTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGt                                                                      <  2:868333/150‑1 (MQ=255)
                                                                                  cAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTg                                                                     <  1:1221995/150‑1 (MQ=255)
                                                                                    aaTCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGcc                                                                   <  2:935353/150‑1 (MQ=255)
                                                                                        cGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCGAACCGCGGCGAGATTGCATTGCGTTTTCTTCGTGCCTGTa                                                               <  2:1185497/150‑1 (MQ=255)
                                                                                         ggTACCGTGAAAGCAATTCTGGGCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTaa                                                              <  2:307631/150‑1 (MQ=255)
                                                                                           tACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAg                                                            >  1:1383122/1‑150 (MQ=255)
                                                                                            aCCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGa                                                           <  2:158024/150‑1 (MQ=255)
                                                                                               gTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGAATTCTTCGTGCCTGTAAAGAACt                                                        >  1:1682713/1‑150 (MQ=255)
                                                                                                 gAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATACTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTgg                                                      >  1:387614/1‑150 (MQ=255)
                                                                                                  aaaGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTggg                                                     <  2:1000772/150‑1 (MQ=255)
                                                                                                       aaTTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCa                                                <  1:713668/150‑1 (MQ=255)
                                                                                                         ttCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAg                                              <  2:786766/150‑1 (MQ=255)
                                                                                                          tCTGTTCGTAATTTGACTACCGGTATATTTTGACGAGTCGCTGGTCTTCATCGAGTAACGAGGACAACATGCAGGATAAAATTGTTATTGCCAACAGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGa                                             <  1:837225/150‑1 (MQ=255)
                                                                                                           cTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGAc                                            >  1:516110/1‑150 (MQ=255)
                                                                                                           cTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGAc                                            <  2:1167992/150‑1 (MQ=255)
                                                                                                             ggTCGAAAGTCGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTg                                          <  2:1394103/150‑1 (MQ=255)
                                                                                                              gTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGt                                         >  1:1650062/1‑150 (MQ=255)
                                                                                                                 gAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGc                                      >  1:1719135/1‑150 (MQ=255)
                                                                                                                  aaaGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCt                                     >  2:810572/1‑150 (MQ=255)
                                                                                                                     gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                                  >  2:269501/1‑150 (MQ=255)
                                                                                                                     gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                                  >  2:1105100/1‑150 (MQ=255)
                                                                                                                     gTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCtgtg                                  <  2:1282964/150‑1 (MQ=255)
                                                                                                                        gACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                               <  2:5595/150‑1 (MQ=255)
                                                                                                                        gACAACCGGTAGAAGTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                               <  1:1681193/150‑1 (MQ=255)
                                                                                                                        gACAACCGGTAGAAATTGACGAGCCGCTGGGCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCAc                               <  2:1505809/150‑1 (MQ=255)
                                                                                                                               ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg                        >  2:473742/1‑150 (MQ=255)
                                                                                                                               ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg                        >  2:1548905/1‑150 (MQ=255)
                                                                                                                               ggTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAgcg                        <  2:1396022/150‑1 (MQ=255)
                                                                                                                                  aGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCgg                     <  1:513220/150‑1 (MQ=255)
                                                                                                                                  aGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCgg                     <  1:1105100/150‑1 (MQ=255)
                                                                                                                                   ctcgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGCCTGTCGCTGTGCACTCCAGCGCGGa                    >  2:218492/6‑150 (MQ=255)
                                                                                                                                    tcgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGAt                   >  1:1351574/5‑150 (MQ=255)
                                                                                                                                    aaTTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATACTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGAt                   <  2:387614/150‑1 (MQ=255)
                                                                                                                                     cgatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTCTTCTTCGTGCCTGTAAAGCACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATc                  >  1:1049629/4‑150 (MQ=255)
                                                                                                                                      gatGACGACCAGCGGCTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGCCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTTTCGCTGTGCACTCCAGCGCGGATcg                 >  2:1151807/3‑150 (MQ=255)
                                                                                                                                        tGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGAGCACTCCAGCGCGGATcgcg               >  2:468119/1‑150 (MQ=255)
                                                                                                                                        tGACGAGCCGCTGGTCGTCATAGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATcgcg               >  1:971328/1‑150 (MQ=255)
                                                                                                                                           cGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATc            <  2:273649/150‑1 (MQ=255)
                                                                                                                                           cGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATc            <  2:1268561/150‑1 (MQ=255)
                                                                                                                                                 gCTCGTCGTCATCGAGTAACGAGCCGAACCTGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAac      <  2:692424/150‑1 (MQ=255)
                                                                                                                                                  cTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAaca     <  1:1355285/150‑1 (MQ=255)
                                                                                                                                                     gtcgtcATCGAGTACCGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGt  <  1:198705/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGAAGTGGGTCAGAAAGTCAACGTGGGCGATACCCTGTGCATCGTTGAAGCCATGAAAATGATGAACCAGATCGAAGCGGACAAATCCGGTACCGTGAAAGCAATTCTGGTCGAAAGTGGACAACCGGTAGAATTTGACGAGCCGCTGGTCGTCATCGAGTAACGAGGCGAACATGCTGGATAAAATTGTTATTGCCAACCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGT  >  NC_000913/3405743‑3406041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: