Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,294 T→A 7.0% intergenic (+28/‑81) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2940TA 7.0% 313.5 / 8.5 112intergenic (+28/‑81)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (49/54);  new base A (4/4);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 116 total aligned reads displayed.

TCATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTG  >  NC_000913/3407145‑3407443
                                                                                                                                                     |                                                                                                                                                     
 catcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAatt                                                                                                                                                      >  1:617661/1‑150 (MQ=255)
   tcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTc                                                                                                                                                    >  1:654372/1‑150 (MQ=255)
    cGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTcc                                                                                                                                                   <  2:941433/150‑1 (MQ=255)
       cGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGc                                                                                                                                                <  2:962639/150‑1 (MQ=255)
       cGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGAAAGGTGCCACATACATCCACAAAATGGAGAAAAAACTCGGGCTTCAGGAAAAATAATACTGCAAAAGCTTCAAAAGGCCTGATATTACGGc                                                                                                                                                >  2:102093/1‑150 (MQ=255)
         gTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCt                                                                                                                                              <  2:121595/150‑1 (MQ=255)
          tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtt                                                                                                                                             <  1:1714607/150‑1 (MQ=255)
           aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCTGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                                                                            <  1:902604/150‑1 (MQ=255)
            tCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttt                                                                                                                                           >  1:1448958/1‑150 (MQ=255)
              aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                         >  2:64308/1‑150 (MQ=255)
              aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttga                                                                                                                                         <  1:630626/150‑3 (MQ=255)
              aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttga                                                                                                                                         <  1:642441/150‑3 (MQ=255)
              aaaaCCAACGTTGATCTGCAGATACGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                         <  2:1328519/150‑1 (MQ=255)
               aaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTtta                                                                                                                                        <  2:411982/150‑1 (MQ=255)
                   cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                                                                    >  2:1541050/1‑150 (MQ=255)
                    aaCGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCAGGCCTTTTTTATTACt                                                                                                                                   >  1:676911/1‑150 (MQ=255)
                      cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCCGAATGGTGGCACTAACATACACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATAACTgg                                                                                                                                 >  2:978870/1‑150 (MQ=255)
                      cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                                 <  2:1396410/150‑1 (MQ=255)
                      cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                                 <  2:1382260/150‑1 (MQ=255)
                      cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCAGCATGGTGGAACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAATACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                                <  2:914591/150‑1 (MQ=255)
                           aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            >  2:1641385/1‑150 (MQ=255)
                           aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  1:732759/150‑1 (MQ=255)
                           aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  2:399602/150‑1 (MQ=255)
                           aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  2:919283/150‑1 (MQ=255)
                           aTCTGCAGATCCGCATCATGAATGACGAGAACTTACAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATCAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  2:849446/150‑1 (MQ=255)
                              tGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTATTACTGGGGATCGACa                                                                                                                        <  1:646814/150‑1 (MQ=255)
                                     ccGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAccccc                                                                                                                  <  2:916161/150‑1 (MQ=255)
                                          tcatGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGTGGATCGACAACCCCCATAAg                                                                                                             >  2:1152555/1‑150 (MQ=255)
                                            atgaatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                           <  1:1070296/150‑1 (MQ=255)
                                            atgaatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                           >  2:479202/1‑150 (MQ=255)
                                               aatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACa                                                                                                        >  1:1194579/1‑150 (MQ=255)
                                                  gaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATc                                                                                                     >  1:1079871/1‑150 (MQ=255)
                                                  gaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATc                                                                                                     >  2:65715/1‑150 (MQ=255)
                                                    cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                                                                                   >  1:542231/1‑150 (MQ=255)
                                                    cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                                                                                   >  1:170945/1‑150 (MQ=255)
                                                    cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTATTATATTACTGGGGATCGACAACCCCCATAAGGTACAAAccc                                                                                                   >  1:288504/1‑150 (MQ=255)
                                                       gaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGc                                                                                                <  1:340469/150‑1 (MQ=255)
                                                          cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCttt                                                                                             >  2:746755/1‑150 (MQ=255)
                                                          cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCttt                                                                                             >  1:537426/1‑150 (MQ=255)
                                                           ttCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttc                                                                                            <  2:357131/150‑1 (MQ=255)
                                                              cAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                                                                         <  2:899855/150‑1 (MQ=255)
                                                                 cATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcc                                                                                      <  1:1391652/150‑1 (MQ=255)
                                                                   tggtggCACTAACATCCACTATCAGGAGAAAAAACGCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCTAAAGTCCGGATTGTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa                                                                                    <  1:257897/150‑1 (MQ=255)
                                                                      tggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCa                                                                                 >  1:1464436/1‑150 (MQ=255)
                                                                      tggCACTAACATCCACACTCTTGAGAAAAAACTCGGTCTTCAGGAAAACTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCa                                                                                 <  2:112763/150‑1 (MQ=255)
                                                                       ggCACTAACATCCCCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAATTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACGCATCAg                                                                                >  2:284967/1‑150 (MQ=255)
                                                                        gCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGTGGATCGACAACCCCCATAAAGTACACTCGCCGCTTTCTTCACCCTACAgg                                                                               >  2:334090/1‑150 (MQ=255)
                                                                         cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                              <  2:198015/150‑1 (MQ=255)
                                                                         cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                              <  2:1284001/150‑1 (MQ=255)
                                                                         cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                              >  1:1245786/1‑150 (MQ=255)
                                                                           cTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGAc                                                                            <  1:1552424/150‑1 (MQ=255)
                                                                               cATCCACTATCTGGAGAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATTAGGTGCAATCCCCGCTTTCTTCACCCATCCGGTACaaaaa                                                                       >  2:70527/1‑150 (MQ=255)
                                                                                 tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTCGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaaa                                                                      >  1:368992/1‑150 (MQ=255)
                                                                                   cACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                                                                    <  2:688831/150‑1 (MQ=255)
                                                                                    aCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCATCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAACCCCATAAGGTACAATCCCAGCTTTCTTCACCCATCAGGGACAAAAAATgg                                                                   >  2:186052/1‑150 (MQ=255)
                                                                                      tATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGac                                                                 <  2:191661/150‑1 (MQ=255)
                                                                                      tATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATAACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGac                                                                 <  1:978870/150‑1 (MQ=255)
                                                                                       aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca                                                                >  1:283933/1‑150 (MQ=255)
                                                                                       aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca                                                                >  2:943736/1‑150 (MQ=255)
                                                                                         cTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTAACCCATCAGGGACAAAAAATGGACACt                                                              <  2:1001177/150‑1 (MQ=255)
                                                                                          tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTc                                                             >  1:1184813/1‑150 (MQ=255)
                                                                                           gtataaaaaaCTCGGTCTTCAGTAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGCATCGACACCCCCCATATGGTACAATCCCCGCTTTCTTCACCCATCCGGTACAAAAAATGGACACTCg                                                            <  2:1590478/146‑1 (MQ=255)
                                                                                             agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt                                                          >  2:357941/1‑150 (MQ=255)
                                                                                             agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt                                                          >  2:779151/1‑150 (MQ=255)
                                                                                              caaaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                         <  1:288947/149‑1 (MQ=255)
                                                                                               aaaaaaCTCGGTCTTCAGGAAAAATAAGACTTCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAATGGGGATCGACAACCCCAATAAGGTACAAGCCACGATTTCTTAAACCATAAGGGACAAAAAATGGAAACTATtttt                                                        >  2:169700/1‑150 (MQ=255)
                                                                                               aaaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtttt                                                        <  1:943736/150‑1 (MQ=255)
                                                                                                aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                       >  2:1562136/1‑150 (MQ=255)
                                                                                                aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGCCAAAAAATGGACACTAGtttag                                                       >  2:1076826/1‑148 (MQ=255)
                                                                                                aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                       <  2:654372/150‑1 (MQ=255)
                                                                                                aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                       >  1:352481/1‑150 (MQ=255)
                                                                                                   aaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTc                                                    <  2:1194579/150‑1 (MQ=255)
                                                                                                      tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAgg                                                 >  2:989566/1‑150 (MQ=255)
                                                                                                             tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaa                                          >  1:455122/1‑150 (MQ=255)
                                                                                                             tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaa                                          >  1:981304/1‑150 (MQ=255)
                                                                                                               aGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAag                                        <  2:1325187/150‑1 (MQ=255)
                                                                                                                ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGTGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                       <  2:676911/150‑1 (MQ=255)
                                                                                                                  aaaaaTAAGACTGCTAAAGCGTTAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAgg                                     >  2:916232/1‑150 (MQ=255)
                                                                                                                    aaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcg                                   <  2:537426/150‑1 (MQ=255)
                                                                                                                     aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATGTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCACATAAGGTACAAACGCCGCTTGAGTCCCACATCAGGGACAAAAAAGGGAACCTAGTTTTGTTCAGGCCAATAAAGAGgcgc                                  >  1:1266881/1‑150 (MQ=255)
                                                                                                                             tGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcg                          >  1:763971/1‑150 (MQ=255)
                                                                                                                              gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcg                          <  1:839945/149‑1 (MQ=255)
                                                                                                                                 aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgctgg                      >  2:629759/1‑150 (MQ=255)
                                                                                                                                  aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACCACCCCCATAAGCTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAACTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGcgctgggcgctggg                     <  1:1331980/150‑1 (MQ=255)
                                                                                                                                   aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                    <  2:1541433/150‑1 (MQ=255)
                                                                                                                                   aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                    >  2:1571917/1‑150 (MQ=255)
                                                                                                                                   aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                    >  1:559170/1‑150 (MQ=255)
                                                                                                                                     cctCAAAAGGCCCGTTTTTCCGGGCTTTTTTATTACCGGGGAACGCCCACCCCCATTACGGACAAACCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCt                  <  1:1541050/148‑1 (MQ=255)
                                                                                                                                     cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTTCAATCCCCGCTTTCGTCACCCATCAGGGACAAAAATTGGACACTCGTTTTGTTCAGGCCCATTAAGAGGCGCGCTGGGCGCTGGGGCt                  >  1:345432/1‑150 (MQ=255)
                                                                                                                                     cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCt                  <  2:1184813/150‑1 (MQ=255)
                                                                                                                                     ataaaaaaGGCCGGAAACTCCGGCCTTTTTTATTACTGGGGATCGACAACCCACATAAGATAAAATCCCCGCATTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGGTGGGGCt                  >  1:1147726/5‑150 (MQ=255)
                                                                                                                                       tCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGa                >  2:667640/1‑150 (MQ=255)
                                                                                                                                        cAAAAGGCCGGATTTTCCGGGCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAc               <  2:170945/150‑1 (MQ=255)
                                                                                                                                         aaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACCCTCGGTTTGTTCAGGCCCATAATGAGGCGCGCTGGGCGCTGGGGCTGagc              >  1:444921/1‑148 (MQ=255)
                                                                                                                                          aaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAccc             >  2:1284344/1‑150 (MQ=255)
                                                                                                                                              gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGCCACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt         <  1:508520/150‑1 (MQ=255)
                                                                                                                                               ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGAGAACCCCCATAAGGCACAATCCCCGCATTCTTCACCCATCACGGACAAAAAATGGACAGTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg        <  2:93954/150‑1 (MQ=255)
                                                                                                                                               ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg        >  2:1439934/1‑150 (MQ=255)
                                                                                                                                                     tttCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTg  >  2:803598/1‑150 (MQ=255)
                                                                                                                                                     tttCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTg  <  2:1679396/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTG  >  NC_000913/3407145‑3407443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: