Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,407,295 T→A 6.5% intergenic (+29/‑80) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,407,2950TA 6.5% 326.3 / 6.0 112intergenic (+29/‑80)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (51/52);  new base A (2/5);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTG  >  NC_000913/3407146‑3407443
                                                                                                                                                     |                                                                                                                                                    
catcGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAatt                                                                                                                                                      >  1:617661/1‑150 (MQ=255)
   cGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTcc                                                                                                                                                   <  2:941433/150‑1 (MQ=255)
      cGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGc                                                                                                                                                <  2:962639/150‑1 (MQ=255)
      cGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGAAAGGTGCCACATACATCCACAAAATGGAGAAAAAACTCGGGCTTCAGGAAAAATAATACTGCAAAAGCTTCAAAAGGCCTGATATTACGGc                                                                                                                                                >  2:102093/1‑150 (MQ=255)
        gTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCt                                                                                                                                              <  2:121595/150‑1 (MQ=255)
         tATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtt                                                                                                                                             <  1:1714607/150‑1 (MQ=255)
          aTCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCTGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttt                                                                                                                                            <  1:902604/150‑1 (MQ=255)
           tCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttt                                                                                                                                           >  1:1448958/1‑150 (MQ=255)
             aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCtttttt                                                                                                                                         >  2:64308/1‑150 (MQ=255)
             aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttga                                                                                                                                         <  1:630626/150‑3 (MQ=255)
             aaaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttga                                                                                                                                         <  1:642441/150‑3 (MQ=255)
              aaaCCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAAAATCCGGCCttttgac                                                                                                                                        <  1:853663/150‑4 (MQ=255)
                  cAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAc                                                                                                                                    >  2:1541050/1‑150 (MQ=255)
                   aaCGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCAGGCCTTTTTTATTACt                                                                                                                                   >  1:676911/1‑150 (MQ=255)
                     cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCCGAATGGTGGCACTAACATACACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATAACTgg                                                                                                                                 >  2:978870/1‑150 (MQ=255)
                     cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                                 <  2:1396410/150‑1 (MQ=255)
                     cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTgg                                                                                                                                 <  2:1382260/150‑1 (MQ=255)
                     cGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCAGCATGGTGGAACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAATACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTggg                                                                                                                                <  2:914591/150‑1 (MQ=255)
                          aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  1:732759/150‑1 (MQ=255)
                          aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            >  2:1641385/1‑150 (MQ=255)
                          aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  2:399602/150‑1 (MQ=255)
                          aTCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  2:919283/150‑1 (MQ=255)
                          aTCTGCAGATCCGCATCATGAATGACGAGAACTTACAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATCAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATc                                                                                                                            <  2:849446/150‑1 (MQ=255)
                             tGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTATTACTGGGGATCGACa                                                                                                                        <  1:646814/150‑1 (MQ=255)
                                    ccGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAccccc                                                                                                                  <  2:916161/150‑1 (MQ=255)
                                          catGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAAcccc                                                                                                                   >  2:1271743/1‑143 (MQ=255)
                                           atgaatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                           >  2:479202/1‑150 (MQ=255)
                                           atgaatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGt                                                                                                           <  1:1070296/150‑1 (MQ=255)
                                              aatgaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACa                                                                                                        >  1:1194579/1‑150 (MQ=255)
                                                 gaCGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATc                                                                                                     >  1:1079871/1‑150 (MQ=255)
                                                   cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATccc                                                                                                   >  1:542231/1‑150 (MQ=255)
                                                   cGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTATTATATTACTGGGGATCGACAACCCCCATAAGGTACAAAccc                                                                                                   >  1:288504/1‑150 (MQ=255)
                                                     agaACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCg                                                                                                 <  1:64308/150‑1 (MQ=255)
                                                         cTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCttt                                                                                             >  2:746755/1‑150 (MQ=255)
                                                          ttCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttc                                                                                            <  2:357131/150‑1 (MQ=255)
                                                          ttCCAGCATGGGGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttc                                                                                            <  1:290854/150‑1 (MQ=255)
                                                             cAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTttcttc                                                                                         <  2:899855/150‑1 (MQ=255)
                                                                cATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCAcc                                                                                      <  1:1391652/150‑1 (MQ=255)
                                                                  tggtggCACTAACATCCACTATCAGGAGAAAAAACGCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCTAAAGTCCGGATTGTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCa                                                                                    <  1:257897/150‑1 (MQ=255)
                                                                     tggCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCa                                                                                 >  1:1464436/1‑150 (MQ=255)
                                                                     tggCACTAACATCCACACTCTTGAGAAAAAACTCGGTCTTCAGGAAAACTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCa                                                                                 <  2:112763/150‑1 (MQ=255)
                                                                      ggCACTAACATCCCCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGAATTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACGCATCAg                                                                                >  2:284967/1‑150 (MQ=255)
                                                                       gCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGTGGATCGACAACCCCCATAAAGTACACTCGCCGCTTTCTTCACCCTACAgg                                                                               >  2:334090/1‑150 (MQ=255)
                                                                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                              >  1:1245786/1‑150 (MQ=255)
                                                                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                              <  2:1284001/150‑1 (MQ=255)
                                                                        cACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAggg                                                                              <  2:198015/150‑1 (MQ=255)
                                                                          cTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGAc                                                                            <  1:1552424/150‑1 (MQ=255)
                                                                              cATCCACTATCTGGAGAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATTAGGTGCAATCCCCGCTTTCTTCACCCATCCGGTACaaaaa                                                                       >  2:70527/1‑150 (MQ=255)
                                                                                tCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTCGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACaaaaaa                                                                      >  1:368992/1‑150 (MQ=255)
                                                                                  cACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATg                                                                    <  2:688831/150‑1 (MQ=255)
                                                                                   aCTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATgg                                                                   <  1:479202/150‑1 (MQ=255)
                                                                                     tATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGac                                                                 <  2:191661/150‑1 (MQ=255)
                                                                                     tATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATAACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGac                                                                 <  1:978870/150‑1 (MQ=255)
                                                                                      aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca                                                                >  1:283933/1‑150 (MQ=255)
                                                                                      aTCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGaca                                                                >  2:943736/1‑150 (MQ=255)
                                                                                       tCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCACGCTTTCTTCACCCATCAGGGACAAAAAATGGacac                                                               >  2:668332/1‑150 (MQ=255)
                                                                                        cTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTAACCCATCAGGGACAAAAAATGGACACt                                                              <  2:1001177/150‑1 (MQ=255)
                                                                                         tGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTc                                                             >  1:1184813/1‑150 (MQ=255)
                                                                                          gtataaaaaaCTCGGTCTTCAGTAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGCATCGACACCCCCCATATGGTACAATCCCCGCTTTCTTCACCCATCCGGTACAAAAAATGGACACTCg                                                            <  2:1590478/146‑1 (MQ=255)
                                                                                            agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt                                                          >  2:357941/1‑150 (MQ=255)
                                                                                            agaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGtt                                                          >  2:779151/1‑150 (MQ=255)
                                                                                             gaAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                         <  1:1639872/150‑1 (MQ=255)
                                                                                             caaaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGttt                                                         <  1:288947/149‑1 (MQ=255)
                                                                                              aaaaaaCTCGGTCTTCAGGAAAAATAAGACTTCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTAATGGGGATCGACAACCCCAATAAGGTACAAGCCACGATTTCTTAAACCATAAGGGACAAAAAATGGAAACTATtttt                                                        >  2:169700/1‑150 (MQ=255)
                                                                                               aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGCCAAAAAATGGACACTAGtttag                                                       >  2:1076826/1‑148 (MQ=255)
                                                                                               aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                       >  1:352481/1‑150 (MQ=255)
                                                                                               aaaaaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTg                                                       <  2:654372/150‑1 (MQ=255)
                                                                                                  aaCTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTc                                                    <  2:1194579/150‑1 (MQ=255)
                                                                                                     tCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAgg                                                 >  2:989566/1‑150 (MQ=255)
                                                                                                            tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaa                                          >  1:455122/1‑150 (MQ=255)
                                                                                                            tCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATaa                                          >  1:981304/1‑150 (MQ=255)
                                                                                                              aGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAag                                        <  2:1325187/150‑1 (MQ=255)
                                                                                                               ggAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGTGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAaga                                       <  2:676911/150‑1 (MQ=255)
                                                                                                                 aaaaaTAAGACTGCTAAAGCGTTAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAgg                                     >  2:916232/1‑150 (MQ=255)
                                                                                                                   aaaTAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGgcg                                   <  2:537426/150‑1 (MQ=255)
                                                                                                                    aaTAAGACTGCTAAAGCGTCAAAAGGCCGGATGTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCACATAAGGTACAAACGCCGCTTGAGTCCCACATCAGGGACAAAAAAGGGAACCTAGTTTTGTTCAGGCCAATAAAGAGgcgc                                  >  1:1266881/1‑150 (MQ=255)
                                                                                                                          aCTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctggg                            <  2:1652099/150‑1 (MQ=255)
                                                                                                                            tGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcg                          >  1:763971/1‑150 (MQ=255)
                                                                                                                             gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcg                          <  1:839945/149‑1 (MQ=255)
                                                                                                                             gCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcg                          >  2:839945/1‑149 (MQ=255)
                                                                                                                                aaaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCgcgctgggcgctgg                      >  2:629759/1‑150 (MQ=255)
                                                                                                                                 aaGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACCACCCCCATAAGCTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAACTGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGcgctgggcgctggg                     <  1:1331980/150‑1 (MQ=255)
                                                                                                                                  aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                    >  2:1571917/1‑150 (MQ=255)
                                                                                                                                  aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                    >  1:559170/1‑150 (MQ=255)
                                                                                                                                  aGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTgggg                    <  2:1541433/150‑1 (MQ=255)
                                                                                                                                    cctCAAAAGGCCCGTTTTTCCGGGCTTTTTTATTACCGGGGAACGCCCACCCCCATTACGGACAAACCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCt                  <  1:1541050/148‑1 (MQ=255)
                                                                                                                                    cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTTCAATCCCCGCTTTCGTCACCCATCAGGGACAAAAATTGGACACTCGTTTTGTTCAGGCCCATTAAGAGGCGCGCTGGGCGCTGGGGCt                  >  1:345432/1‑150 (MQ=255)
                                                                                                                                    cGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCt                  <  2:1184813/150‑1 (MQ=255)
                                                                                                                                    ataaaaaaGGCCGGAAACTCCGGCCTTTTTTATTACTGGGGATCGACAACCCACATAAGATAAAATCCCCGCATTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGGTGGGGCt                  >  1:1147726/5‑150 (MQ=255)
                                                                                                                                    ataaaaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCAATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTATGTTCAGGCCCATAAAGAGGAGCGCTGGGCGATggggat                  >  2:1075569/5‑148 (MQ=255)
                                                                                                                                      tCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGa                >  2:667640/1‑150 (MQ=255)
                                                                                                                                       cAAAAGGCCGGATTTTCCGGGCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCCCCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAc               <  2:170945/150‑1 (MQ=255)
                                                                                                                                        aaaaGGCCGGAAAATCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACCCTCGGTTTGTTCAGGCCCATAATGAGGCGCGCTGGGCGCTGGGGCTGagc              >  1:444921/1‑148 (MQ=255)
                                                                                                                                         aaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAccc             >  2:1284344/1‑150 (MQ=255)
                                                                                                                                         aaaGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGAccc             >  2:43108/1‑150 (MQ=255)
                                                                                                                                             gCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGCCACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCtttt         <  1:508520/150‑1 (MQ=255)
                                                                                                                                              ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGAGAACCCCCATAAGGCACAATCCCCGCATTCTTCACCCATCACGGACAAAAAATGGACAGTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg        <  2:93954/150‑1 (MQ=255)
                                                                                                                                              ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGATAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg        <  2:1448958/150‑1 (MQ=255)
                                                                                                                                              ccGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTg        >  2:1439934/1‑150 (MQ=255)
                                                                                                                                                    tttCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTg  <  2:1679396/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CATCGACGGTATCAAAACCAACGTTGATCTGCAGATCCGCATCATGAATGACGAGAACTTCCAGCATGGTGGCACTAACATCCACTATCTGGAGAAAAAACTCGGTCTTCAGGAAAAATAAGACTGCTAAAGCGTCAAAAGGCCGGATTTTCCGGCCTTTTTTATTACTGGGGATCGACAACCCCCATAAGGTACAATCCCCGCTTTCTTCACCCATCAGGGACAAAAAATGGACACTCGTTTTGTTCAGGCCCATAAAGAGGCGCGCTGGGCGCTGGGGCTGACCCTTTTGTATCTG  >  NC_000913/3407146‑3407443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: