Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,443,592 A→C 7.7% M169R (ATG→AGG) ‡ secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,5920AC 7.7% 453.5 / 21.6 153M169R (ATG→AGG) ‡secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (68/73);  new base C (7/5);  total (75/78)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 154 total aligned reads displayed.

CGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGG  >  NC_000913/3443443‑3443739
                                                                                                                                                     |                                                                                                                                                   
  aGGAAGTGCAGGTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATg                                                                                                                                                   >  2:277553/1‑150 (MQ=255)
     taGTGCAGGTGTCCTTGAAGCGGTTGGTCGATAGTATGGGCAATGGCTGGCTGGAGTCCCGCGACATTACGGGCGTTGATAATGATTGTAATACCGTTGCCGATACCTAGTTCAGTAATCTGTTGGCCCAACCACATGAGGAACATGGtt                                                                                                                                                <  1:871891/149‑1 (MQ=255)
       gTGCAGGTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTcc                                                                                                                                              <  1:844483/150‑1 (MQ=255)
         gCAGGTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCGCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCtg                                                                                                                                            >  1:1686297/1‑150 (MQ=255)
         gCAGGTCGCCTTGACGCGCTTGCGCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCtg                                                                                                                                            <  2:618993/150‑1 (MQ=255)
          cAGGTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACCATGTTCCtga                                                                                                                                           <  1:1572165/150‑2 (MQ=255)
            ggTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACCATGTTCCtgatg                                                                                                                                         <  2:217879/150‑4 (MQ=255)
            ggTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACCATGTTCCtgatg                                                                                                                                         <  2:1564906/150‑4 (MQ=255)
             gTCGCCTTGACGCGCTTGCTCGATAGTATGTGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGAc                                                                                                                                        <  1:106090/150‑1 (MQ=255)
               cGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCa                                                                                                                                      >  1:545471/1‑150 (MQ=255)
                gCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAg                                                                                                                                     >  1:1101583/1‑150 (MQ=255)
                 ccTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGa                                                                                                                                    >  1:935809/1‑150 (MQ=255)
                  cTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGCTAAGGCTTGAAATACCGGTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACCTCAGGAACATGGTTCCTGTGACCAGAc                                                                                                                                   >  1:121301/1‑150 (MQ=255)
                      aCGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTac                                                                                                                               <  1:277553/150‑1 (MQ=255)
                       cgcgCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTaca                                                                                                                              <  2:1542003/150‑1 (MQ=255)
                       cgcgCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTaca                                                                                                                              >  1:1297551/1‑150 (MQ=255)
                       cgcgCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTaca                                                                                                                              <  2:884992/150‑1 (MQ=255)
                        gcgcTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTacaa                                                                                                                             <  1:1049666/150‑1 (MQ=255)
                         cgcTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTacaac                                                                                                                            >  1:686225/1‑150 (MQ=255)
                              gCTCGATAGTATGGTCAATGTCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCgg                                                                                                                       <  1:314371/150‑1 (MQ=255)
                              gCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCgg                                                                                                                       <  2:57511/150‑1 (MQ=255)
                              gCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCgg                                                                                                                       >  2:1385899/1‑150 (MQ=255)
                                tCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGAACAGACTTCCACCAGCGGTg                                                                                                                     >  2:89143/1‑150 (MQ=255)
                                 cGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGa                                                                                                                    <  1:1385899/150‑1 (MQ=255)
                                    tAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGt                                                                                                                 <  2:1570699/150‑1 (MQ=255)
                                          gggCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATg                                                                                                           <  1:260966/150‑1 (MQ=255)
                                          gggCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATg                                                                                                           <  2:431419/150‑1 (MQ=255)
                                                  gctggcGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTac                                                                                                                               >  1:240236/1‑122 (MQ=255)
                                                      gcGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGt                                                                                               <  1:437398/150‑1 (MQ=255)
                                                      gcGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGt                                                                                               >  1:10630/1‑150 (MQ=255)
                                                       cGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGtt                                                                                              <  2:353043/150‑1 (MQ=255)
                                                       cGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGtt                                                                                              >  1:1390062/1‑150 (MQ=255)
                                                       cGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGtt                                                                                              >  1:418396/1‑150 (MQ=255)
                                                        gggAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTAGAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTa                                                                                             <  2:133652/150‑1 (MQ=255)
                                                            ggcccGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCa                                                                                         <  1:142663/148‑1 (MQ=255)
                                                               ccGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCa                                                                                      <  1:1017609/150‑1 (MQ=255)
                                                                     cAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCtt                                                                                >  1:410803/1‑150 (MQ=255)
                                                                        tACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCa                                                                             <  2:799927/150‑1 (MQ=255)
                                                                         aCCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCAt                                                                            >  1:173263/1‑150 (MQ=255)
                                                                         aCCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCAt                                                                            <  2:24452/150‑1 (MQ=255)
                                                                          ccGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTTATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATa                                                                           <  1:13272/150‑1 (MQ=255)
                                                                          ccGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACAACATCAGGAACATGGTTCCTGTGACCAGACTTACAACATCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATa                                                                           <  1:1115608/150‑1 (MQ=255)
                                                                            ggCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATAcc                                                                         <  1:513660/150‑1 (MQ=255)
                                                                            ggCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATAcc                                                                         >  2:1399445/1‑150 (MQ=255)
                                                                              cGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAACGCCCGGGTTAATCACCAGGCCTTGCATACCAg                                                                       >  2:946380/1‑150 (MQ=255)
                                                                              cGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAg                                                                       <  1:537861/150‑1 (MQ=255)
                                                                              cGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAg                                                                       >  2:1624805/1‑150 (MQ=255)
                                                                                    tAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCatat                                                                 >  1:384538/1‑150 (MQ=255)
                                                                                    tAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCatat                                                                 >  2:1717783/1‑150 (MQ=255)
                                                                                      aTGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTc                                                               <  1:1399445/150‑1 (MQ=255)
                                                                                       tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCg                                                              >  2:267422/1‑150 (MQ=255)
                                                                                       tGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCg                                                              >  1:1523941/1‑150 (MQ=255)
                                                                                         aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGc                                                            <  1:1013549/150‑1 (MQ=255)
                                                                                         aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGc                                                            <  2:1277542/150‑1 (MQ=255)
                                                                                         aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGc                                                            <  1:691926/150‑1 (MQ=255)
                                                                                         aTTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGc                                                            <  2:758020/150‑1 (MQ=255)
                                                                                          ttGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCa                                                           <  2:1101583/150‑1 (MQ=255)
                                                                                            gAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGa                                                         >  2:1328818/1‑150 (MQ=255)
                                                                                            gAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGa                                                         >  1:957810/1‑150 (MQ=255)
                                                                                             aaaTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCCCCAGGCCTTGCATACCAGGCATATTCGGCAGAc                                                        >  1:108342/1‑150 (MQ=255)
                                                                                             aaaTACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGAc                                                        <  2:684138/150‑1 (MQ=255)
                                                                                                tACCGTTGCCGATACCTCGTTCAGTAATCTGGTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTCAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCgg                                                     <  2:937379/150‑1 (MQ=255)
                                                                                                  ccGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTGCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTa                                                   <  2:1085087/150‑1 (MQ=255)
                                                                                                    gTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGGGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGc                                                 <  2:1105218/150‑1 (MQ=255)
                                                                                                    gTTGCCGAAACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGc                                                 <  1:1103568/150‑1 (MQ=255)
                                                                                                       gCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAAt                                              >  2:1534819/1‑150 (MQ=255)
                                                                                                        ccGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATa                                             <  2:586830/150‑1 (MQ=255)
                                                                                                        ccGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATa                                             >  1:1607074/1‑150 (MQ=255)
                                                                                                          gATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTg                                                                               <  2:441066/114‑1 (MQ=255)
                                                                                                          gATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATAcc                                           <  1:1152102/150‑1 (MQ=255)
                                                                                                           aTACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATCCCg                                          >  2:762158/1‑150 (MQ=255)
                                                                                                              ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                       >  2:819135/1‑150 (MQ=255)
                                                                                                              ccTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATc                                       >  2:1037756/1‑150 (MQ=255)
                                                                                                                tCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGGGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGa                                     <  1:1218421/150‑1 (MQ=255)
                                                                                                                 cGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGAc                                    >  1:1186162/1‑150 (MQ=255)
                                                                                                                    tCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTgg                                 >  2:591486/1‑150 (MQ=255)
                                                                                                                     cAGTAATCTGTTCGCCAAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGACATATTCGGCAGACCGGTAGCAATACCGATCGACTGGa                                >  2:238389/1‑150 (MQ=255)
                                                                                                                      aGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGaa                               >  1:480774/1‑150 (MQ=255)
                                                                                                                             tGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGcc                        >  2:1581377/1‑150 (MQ=255)
                                                                                                                             tGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGcc                        >  2:647335/1‑150 (MQ=255)
                                                                                                                              gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGAt                                        >  2:966241/1‑133 (MQ=255)
                                                                                                                              gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGAt                                        <  1:966241/133‑1 (MQ=255)
                                                                                                                              gTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCa                       <  1:1037756/150‑1 (MQ=255)
                                                                                                                                  gCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCAc                   >  1:790135/1‑150 (MQ=255)
                                                                                                                                   cccAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCAcc                  <  2:545471/150‑1 (MQ=255)
                                                                                                                                   cccAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCAcc                  >  1:1252256/1‑150 (MQ=255)
                                                                                                                                     cAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCag                <  1:1534819/150‑1 (MQ=255)
                                                                                                                                         cacaTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTa            >  2:1118495/1‑150 (MQ=255)
                                                                                                                                            cacAGGAACCATGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCg         >  1:654023/3‑150 (MQ=255)
                                                                                                                                            cacAGGAACCATGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCg         >  1:223080/3‑150 (MQ=255)
                                                                                                                                            cacAGGAACCATGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACAAGAGTACCg         >  1:1505503/3‑150 (MQ=255)
                                                                                                                                            aTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCg         >  1:644339/1‑150 (MQ=255)
                                                                                                                                            aTCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCg         >  1:913695/1‑150 (MQ=255)
                                                                                                                                             acAGGAACCATGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGt        >  1:1029829/2‑150 (MQ=255)
                                                                                                                                             acAGGAACCATGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGAACCGt        >  1:1700992/2‑150 (MQ=255)
                                                                                                                                              cAGGAACCATGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGtt       >  1:245823/1‑149 (MQ=255)
                                                                                                                                              cAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTa       >  2:1301648/1‑150 (MQ=255)
                                                                                                                                               aGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAg      <  1:86446/150‑1 (MQ=255)
                                                                                                                                                 gAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCg    >  1:758025/1‑150 (MQ=255)
                                                                                                                                                   aCATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCggg  <  1:1238477/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGAGGAAGTGCAGGTCGCCTTGACGCGCTTGCTCGATAGTATGGGCAATGGCTGGCGGGAGTCCCGCGACAATACCGGCGAAGATAATGATTGAAATACCGTTGCCGATACCTCGTTCAGTAATCTGTTCGCCCAACCACATCAGGAACATGGTTCCTGTGACCAGACTTACAACAGCGGTGAAGTAGAATGCAAAGCCCGGGTTAATCACCAGGCCTTGCATACCAGGCATATTCGGCAGACCGGTAGCAATACCGATCGACTGGAATATTGCCAGCACCAGAGTACCGTAGCGGG  >  NC_000913/3443443‑3443739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: