Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,544,689 A→T 5.5% R52R (CGT→CGA bioH ← pimeloyl‑ACP methyl ester carboxylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,544,6890AT 5.5% 450.6 / 10.4 156R52R (CGT→CGAbioHpimeloyl‑ACP methyl ester carboxylesterase
Reads supporting (aligned to +/- strand):  ref base A (74/73);  new base T (5/4);  total (79/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 156 total aligned reads displayed.

CGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGAT  >  NC_000913/3544542‑3544835
                                                                                                                                                   |                                                                                                                                                  
cgcCTGAACACGCTCGGGATGGGTTAACGCAATCTGCCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACg                                                                                                                                                  <  2:1002226/150‑1 (MQ=255)
 gcCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGc                                                                                                                                                 <  1:1415319/150‑1 (MQ=255)
    tGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCg                                                                                                                                              <  1:52383/150‑1 (MQ=255)
     gAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCCCCAAATCCCCGGCTACGCCCGa                                                                                                                                             >  1:475079/1‑150 (MQ=255)
         acGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGcc                                                                                                                                         >  2:1609071/1‑150 (MQ=255)
            cTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTTCGGGCGTAGCCggg                                                                                                                                      <  2:944834/150‑1 (MQ=255)
             tCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGc                                                                                                                                     <  2:975654/150‑1 (MQ=255)
               gggATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAg                                                                                                                                   >  2:1544733/1‑150 (MQ=255)
                  aggggTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATAGCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTc                                                                                                                                <  1:320312/148‑1 (MQ=255)
                  aTGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTc                                                                                                                                >  2:224957/1‑150 (MQ=255)
                    gggTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaa                                                                                                                              <  1:1310443/150‑1 (MQ=255)
                     ggTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaac                                                                                                                             >  2:1305433/1‑150 (MQ=255)
                        tAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTcaacatg                                                                                                                          >  1:509963/1‑148 (MQ=255)
                        tAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAg                                                                                                                          >  2:99272/1‑150 (MQ=255)
                         aaCGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAgg                                                                                                                         >  1:55179/1‑150 (MQ=255)
                          aCGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGt                                                                                                                        <  1:1483578/150‑1 (MQ=255)
                          aCGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGt                                                                                                                        <  1:899263/150‑1 (MQ=255)
                             cAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCACATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCa                                                                                                                     >  2:1165530/1‑150 (MQ=255)
                              aaTCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAg                                                                                                                    <  2:1456042/150‑1 (MQ=255)
                              aaTCTGGCTTGCCACCAGCCCGACCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAg                                                                                                                    <  2:311674/150‑1 (MQ=255)
                                  tGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTa                                                                                                                >  2:1421628/1‑150 (MQ=255)
                                   ggCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTTCGGGCGTAGCCgggg                                                                                                                                     >  1:1554231/1‑127 (MQ=255)
                                        gCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGc                                                                                                          <  1:364810/150‑1 (MQ=255)
                                         ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCg                                                                                                         <  2:740594/150‑1 (MQ=255)
                                         ccaccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCg                                                                                                         >  2:937176/1‑150 (MQ=255)
                                            ccaGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGc                                                                                                      >  1:1325399/1‑150 (MQ=255)
                                               gcccgcccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTaa                                                                                                   >  1:1597866/1‑150 (MQ=255)
                                               gcccgcccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCATCGGCCATATCAGCAAGTGACAGCGCACCAAATCGCCGGCTACGCCCGACGCCGGGCAGGTCCACAAGGTGCAGCGTACAATGCGAGCTaa                                                                                                   >  1:263159/1‑150 (MQ=255)
                                                  cgcccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGCCCGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGtt                                                                                                >  2:1252083/1‑150 (MQ=255)
                                                   gcccAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAACCCGGGCAGGTCAACAAGGTGCAGCGAAAAATGCGAGCTAAGTTc                                                                                               >  1:1258373/1‑150 (MQ=255)
                                                          cTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCa                                                                                        >  2:1258233/1‑150 (MQ=255)
                                                              aGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGc                                                                                    >  1:1145195/1‑150 (MQ=255)
                                                              aGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGc                                                                                    <  2:957657/150‑1 (MQ=255)
                                                               gCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCa                                                                                   >  2:1165935/1‑150 (MQ=255)
                                                               gCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCa                                                                                   <  1:1410951/150‑1 (MQ=255)
                                                                ccTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCaa                                                                                  <  1:1529479/150‑1 (MQ=255)
                                                                ccTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCaa                                                                                  <  2:900200/150‑1 (MQ=255)
                                                                  tAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACg                                                                                <  1:164432/150‑1 (MQ=255)
                                                                       aaaTGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCcaca                                                                           <  1:828559/150‑1 (MQ=255)
                                                                             cTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTctg                                                                     <  2:1210495/150‑3 (MQ=255)
                                                                             cTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCgg                                                                     >  1:1545908/1‑150 (MQ=255)
                                                                              tttATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGACGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGc                                                                    >  1:104161/1‑150 (MQ=255)
                                                                              tttATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGc                                                                    <  1:194444/150‑1 (MQ=255)
                                                                              tttATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTAGTCAATGCAACGCCACACTTCGGc                                                                    >  1:1609628/1‑150 (MQ=255)
                                                                                tATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCAt                                                                  >  1:165920/1‑150 (MQ=255)
                                                                                tATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCAt                                                                  >  1:1103947/1‑150 (MQ=255)
                                                                                  tCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCATGGGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTc                                                                <  1:437031/150‑1 (MQ=255)
                                                                                  tCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTc                                                                <  2:266932/150‑1 (MQ=255)
                                                                                   cAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCa                                                               >  2:178580/1‑150 (MQ=255)
                                                                                   cAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCa                                                               >  1:221394/1‑150 (MQ=255)
                                                                                        gCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCCCACTTCGGCATTCAGTccc                                                          >  2:908165/1‑150 (MQ=255)
                                                                                        gCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCAACACTTCGGCATTCAGTccc                                                          >  2:39471/1‑150 (MQ=255)
                                                                                        gCCTGTTGCAGCACGGCTTCGCCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCATCGCCACACTTCGGCATTCAGTccc                                                          >  2:1483980/1‑150 (MQ=255)
                                                                                          cTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGTGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCa                                                        <  1:940253/150‑1 (MQ=255)
                                                                                              tGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCg                                                    <  1:735020/150‑1 (MQ=255)
                                                                                              tGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCg                                                    <  2:1325399/150‑1 (MQ=255)
                                                                                                cagcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTg                                                  >  2:1639140/1‑150 (MQ=255)
                                                                                                cagcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTg                                                  >  2:3110/1‑150 (MQ=255)
                                                                                                 agcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgc                                                 >  1:967601/1‑150 (MQ=255)
                                                                                                 agcaCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGGAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgc                                                 >  1:52520/1‑150 (MQ=255)
                                                                                                 agcaCGGATTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCACGGATACGCCCGAAGCGGGGCAGGTCAACAAGGAGCAGAGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGCCATTCAGTCCCCATCCGTgc                                                 >  1:1071762/1‑150 (MQ=255)
                                                                                                   caCGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCATCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTgcag                                               <  2:607716/150‑1 (MQ=255)
                                                                                                            ggCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAg                                      >  1:678355/1‑150 (MQ=255)
                                                                                                            ggCCATATCAGCAAGGGACAGCGCACCAAATCCCCGGCGACGCCCGAAGCCGTGCAGGTCAACAAGGTGCAGCGTAATATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAg                                      <  2:838874/150‑1 (MQ=255)
                                                                                                             gCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGa                                     >  2:1119426/1‑150 (MQ=255)
                                                                                                              ccATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGAt                                    >  1:1422327/1‑150 (MQ=255)
                                                                                                                atatCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGa                                  >  2:566376/1‑150 (MQ=255)
                                                                                                                 tatCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCTGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGGAGCACAAGATgca                                 >  1:334218/1‑148 (MQ=255)
                                                                                                                 tatCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAGAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATc                                                      >  2:785267/1‑129 (MQ=255)
                                                                                                                 tatCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGaa                                 >  1:946299/1‑150 (MQ=255)
                                                                                                                    cAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGAGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACAt                              >  1:106093/1‑150 (MQ=255)
                                                                                                                     aGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACAtt                             <  1:99272/150‑1 (MQ=255)
                                                                                                                      gCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGGGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTc                            <  2:106093/150‑1 (MQ=255)
                                                                                                                       cAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcc                           <  2:55179/150‑1 (MQ=255)
                                                                                                                       cAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcc                           >  1:1187440/1‑150 (MQ=255)
                                                                                                                        aaGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTccc                          <  2:1545908/150‑1 (MQ=255)
                                                                                                                        aaGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTccc                          <  2:419297/150‑1 (MQ=255)
                                                                                                                         tgTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcccc                         <  1:156581/149‑1 (MQ=255)
                                                                                                                         aGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcccc                         <  2:615271/150‑1 (MQ=255)
                                                                                                                         aGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcccc                         >  2:1593639/1‑150 (MQ=255)
                                                                                                                         aGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcccc                         <  1:1305433/150‑1 (MQ=255)
                                                                                                                         aGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTcccc                         <  1:224957/150‑1 (MQ=255)
                                                                                                                          gTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCt                        <  1:549559/150‑1 (MQ=255)
                                                                                                                              cAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGAcc                    <  2:165920/150‑1 (MQ=255)
                                                                                                                              aaGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGAcc                    <  1:1037444/149‑1 (MQ=255)
                                                                                                                                 cgcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCTTCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCttt                 <  1:1115647/150‑1 (MQ=255)
                                                                                                                                 cgcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCttt                 <  2:493774/150‑1 (MQ=255)
                                                                                                                                 cgcACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCttt                 >  1:910637/1‑150 (MQ=255)
                                                                                                                                   cACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTgg               >  2:882591/1‑150 (MQ=255)
                                                                                                                                      cAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCt            <  2:1145195/150‑1 (MQ=255)
                                                                                                                                      cAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCt            <  1:882591/150‑1 (MQ=255)
                                                                                                                                       aaaTCCCCGGCTATGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAAAATTCCCCTGACCTTTGGTCTg           >  2:1087469/1‑150 (MQ=255)
                                                                                                                                         ctgcccGGCTTCGGGCGTAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc         >  2:15656/4‑150 (MQ=255)
                                                                                                                                         ctgcccGGCTTCGGGCGTAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc         >  1:1713460/4‑150 (MQ=255)
                                                                                                                                         aTCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcc         <  1:1473832/150‑1 (MQ=255)
                                                                                                                                          tgcccGGCTTCGGGCGTAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGcca        >  2:739042/3‑150 (MQ=255)
                                                                                                                                             ccGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGccacca     >  1:865616/1‑150 (MQ=255)
                                                                                                                                             ccGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAACATGAACATTCCCCTGCCCTTTGGTCTGccacca     >  2:1504394/1‑150 (MQ=255)
                                                                                                                                               ggCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGa   >  2:1616100/1‑150 (MQ=255)
                                                                                                                                                gCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGAt  <  2:1522375/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCCTGAACACGCTCGGGATGGGTTAACGCAATCTGGCTTGCCACCAGCCCGCCCAGACTCCAGCCTAACCAAATGGCTTTATCAGGTGCCTGTTGCAGCACGGCTTCGGCCATATCAGCAAGTGACAGCGCACCAAATCCCCGGCTACGCCCGAAGCCGGGCAGGTCAACAAGGTGCAGCGTAAAATGCGAGCTAAGTTCCTCGTCAATGCAACGCCACACTTCGGCATTCAGTCCCCATCCGTGCAGCAGCACAAGATGAACATTCCCCTGACCTTTGGTCTGCCACCAGAT  >  NC_000913/3544542‑3544835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: