BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,566,210	A→T	9.2%	G124G (GGT→GGA) ‡	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,210	0	A	T	9.2%	248.4 / 12.3	107	G124G (GGT→GGA) ‡	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base A (43/50); new base T (5/5); total (52/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 107 total aligned reads displayed.

CCAGCCAGTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACA  >  NC_000913/3566062‑3566358

ccagcccgTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCAAAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCAc                                                                                                                                                     <  2:123408/150‑1 (MQ=255)

ccagccagTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCAc                                                                                                                                                     >  1:935920/1‑150 (MQ=255)

     cagTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTa                                                                                                                                                >  2:1658682/1‑150 (MQ=255)

     cagTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTa                                                                                                                                                >  2:408981/1‑150 (MQ=255)

       gtagtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTAcc                                                                                                                                              <  2:1669520/150‑1 (MQ=255)

         agtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCGAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGa                                                                                                                                            >  2:1198360/1‑150 (MQ=255)

         agtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGATTCAAGGAAGCAAGCCGCCAGACGTCCCCGGCCACCGTTACCgc                                                                                                                                            >  1:102511/1‑149 (MQ=255)

          gtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAg                                                                                                                                           >  2:1325676/1‑150 (MQ=255)

          gtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAg                                                                                                                                           <  1:614001/150‑1 (MQ=255)

           tCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAgg                                                                                                                                          <  1:13902/150‑1 (MQ=255)

              ggCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCt                                                                                                                                       >  1:1636943/1‑150 (MQ=255)

                cGACTCTATCTGGCTACCGTTAACGATGATCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTgg                                                                                                                                     <  2:856076/150‑1 (MQ=255)

                  aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATAGCGTATTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCTAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                   <  1:1279048/150‑2 (MQ=255)

                  aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                   <  1:1223626/150‑2 (MQ=255)

                  aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                   <  1:1709580/150‑2 (MQ=255)

                  aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                   <  1:1703084/150‑2 (MQ=255)

                  aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGt                                                                                                                                   <  2:528781/150‑1 (MQ=255)

                   ctctTTCTGGCTACCGTTTACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGCGGCCTGGGTc                                                                                                                                  >  2:560515/1‑150 (MQ=255)

                     ctTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAt                                                                                                                                >  1:653089/1‑150 (MQ=255)

                          tGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                           <  1:471182/150‑1 (MQ=255)

                            gCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                         >  2:1107968/1‑150 (MQ=255)

                            gCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                         <  1:548793/150‑1 (MQ=255)

                            gCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                         <  2:1594404/150‑1 (MQ=255)

                             cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTg                                                                                                                                      >  2:1324496/1‑136 (MQ=255)

                             cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTg                                                                                                                                      <  1:1324496/136‑1 (MQ=255)

                             cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtca                                                                                                                        >  2:546367/1‑150 (MQ=255)

                               aCCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcatc                                                                                                                      <  2:1273036/150‑1 (MQ=255)

                                ccGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGGCTGGGTCATTTTAGtcatca                                                                                                                     >  2:1523504/1‑150 (MQ=255)

                                ccGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcatca                                                                                                                     >  1:126056/1‑150 (MQ=255)

                                  gTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTAACGAGGCCTGGGTCATTttcttaaaaaat                                                                                                                   >  2:1499144/1‑143 (MQ=255)

                                   ttAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaatc                                                                                                                  <  1:888766/150‑1 (MQ=255)

                                    tAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaatca                                                                                                                 <  1:546367/150‑1 (MQ=255)

                                      aCGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGc                                                                                                               <  2:213204/150‑1 (MQ=255)

                                          tGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCtt                                                                                                           <  1:1325676/150‑1 (MQ=255)

                                            ttCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCg                                                                                                         <  2:935920/150‑1 (MQ=255)

                                              cTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGTCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCGGGGTCATTTTCTTCGTCGATCAGCTCTTCgag                                                                                                       >  1:664106/1‑150 (MQ=255)

                                               tGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCgaga                                                                                                      <  1:105542/150‑1 (MQ=255)

                                                gCTTGAACATACCGTAGTCATAGCGGATGCCGAAACCGCGCCCCGGCAACCCTAACTTCGCCAGAGATTCAAGGAAGCAAGCCGCCAGACGTCCCAGCCCACCCTTCCCGAGGCCTGGGTCCATTTCTTCATCAAACCGCTCTTCgccaa                                                                                                     >  1:1446398/1‑146 (MQ=255)

                                                   tGAACATACCGTAGTCATAGCGGAGGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTa                                                                                                  <  1:702440/150‑1 (MQ=255)

                                                     aaCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGAAATCCGCCAGACGTCCGAGGCCTCGGGTACCGAGGGCTGGGTGATTTTATTCATAAATCAGGTCTTCGAGATTTTAc                                                                                                >  2:1372042/1‑150 (MQ=255)

                                                      aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAcc                                                                                               <  1:1039068/150‑1 (MQ=255)

                                                       cATACCGTTGTCATTGCGGATGCCGTATCCGCGCCGCGGCATCCCTAACGTCGCAAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAccc                                                                                              <  1:843290/150‑1 (MQ=255)

                                                       cATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAccc                                                                                              >  2:567831/1‑150 (MQ=255)

                                                         tACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGCGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCa                                                                                            >  2:1059306/1‑150 (MQ=255)

                                                              tAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCt                                                                                       <  1:650175/150‑1 (MQ=255)

                                                                 tCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTcc                                                                                    <  1:952350/150‑1 (MQ=255)

                                                                  cATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCa                                                                                   <  1:1658682/150‑1 (MQ=255)

                                                                  cATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTATTCATCAATCAGCTCTTCGAGTTTTAACCCCATCGCTTCCa                                                                                   >  2:569220/1‑150 (MQ=255)

                                                                    tAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCCGGCCCCCGGTGCCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAGTTAACCCCCTCGCTTCCAgg                                                                                 >  1:599783/1‑149 (MQ=255)

                                                                     aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGGCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                <  2:126056/150‑1 (MQ=255)

                                                                     aGCGGATGACGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                <  1:1499144/150‑1 (MQ=255)

                                                                       cGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTgcg                                                                              <  2:592026/150‑1 (MQ=255)

                                                                                   ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCt                                                                  >  1:659275/1‑150 (MQ=255)

                                                                                    cgcgCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCGCTTCGAGAGTTAACCCCATCGCTTCCAGGGCGCCCTGGACATcgt                                                                 >  1:667805/1‑148 (MQ=255)

                                                                                       gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGt                                                              >  2:428745/1‑150 (MQ=255)

                                                                                       gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGt                                                              >  2:422461/1‑150 (MQ=255)

                                                                                          ccGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTaaa                                                           >  2:202342/1‑150 (MQ=255)

                                                                                           cGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCACGCCACCCTTACCGCGGCCTGGGTCAATTAATACATCAATCAGCACTTCGAAAATTAACCCCATAGCGTCCACAGCCCCCTGTACATCTTCGTaaaa                                                          >  2:1081158/1‑149 (MQ=255)

                                                                                              cAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACTTCCCAGGCCACCGTTACCGAGGCCTGGGGCAGTTTCGTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCATCCAGTGCGCCCTGTACAACTTCGTAAATTcg                                                       >  2:1047661/1‑149 (MQ=255)

                                                                                                 cccTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAg                                                    <  1:849016/150‑1 (MQ=255)

                                                                                                 cccTAACGTCGCCAGAGAATCAAGGAAGAAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTACATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAg                                                    >  2:1111186/1‑150 (MQ=255)

                                                                                                     aaCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGac                                                <  1:1623750/150‑1 (MQ=255)

                                                                                                     aaCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGac                                                >  1:1218032/1‑150 (MQ=255)

                                                                                                      aCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGaca                                               <  1:941605/150‑1 (MQ=255)

                                                                                                       cGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTTCCGAGGCCTGGGTCATTTTCTTCATAAATCAGCTCGTCGAGATTTAACCCCATCGCTTACAGTGCGCCCTGTACCTCTTCGTAAATTCCTAGCGacca                                              >  2:591480/1‑148 (MQ=255)

                                                                                                        gTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaac                                             <  1:312417/150‑1 (MQ=255)

                                                                                                           gCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATg                                          <  2:653089/150‑1 (MQ=255)

                                                                                                            ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATgg                                         <  1:1143474/150‑1 (MQ=255)

                                                                                                            ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCCTTACCGCGGCCTGGGGCATTTTCGTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTTCGCCCGGTACAACGTCGTAAATTCCTAGCGACAACAAgg                                         >  2:1590780/1‑150 (MQ=255)

                                                                                                             cAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGATGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGAGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGc                                        <  2:1335343/150‑1 (MQ=255)

                                                                                                               gagaATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGt                                      <  1:1059306/150‑1 (MQ=255)

                                                                                                                  aaTCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                   >  1:188932/1‑150 (MQ=255)

                                                                                                                     cAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCCTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgaga                                >  2:1658828/1‑150 (MQ=255)

                                                                                                                       aGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                              <  1:408981/150‑1 (MQ=255)

                                                                                                                       aGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                              <  2:188932/150‑1 (MQ=255)

                                                                                                                        ggAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGTGAGCg                             >  1:62462/1‑150 (MQ=255)

                                                                                                                         gAAGCAAGCAGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCATCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGt                            <  1:258281/150‑1 (MQ=255)

                                                                                                                            gcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTATTCAAAAATCACCTCTTCGAGATTTAACCCCAACGCTTCCAGTGCGCCCAGTACATCTTCGTAAATTACTAGCGACAACATCGCGTTGGAGAGCGTACg                         >  2:1686778/1‑150 (MQ=255)

                                                                                                                              aagcCGCCAGACGTCCCAGGCCACCGTTAACGACGCCTGGGTCATTTTGTTCAACAATAAGCTATTAGAGATTTTAACCCATAGCTTACACTGAGCCCGGTTAAAATTAGTAAAATTATAGCGACAACAAGGAGGTTGGGAGCGTaaagc                       >  2:1422052/1‑146 (MQ=255)

                                                                                                                                gccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAGTTCCCCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAGTCCTAGCGACAACATGGCGGTGGCGAGCGTACGGCCa                     >  1:17985/1‑150 (MQ=255)

                                                                                                                                  cgccAGTCGTCCCAGGCCACCGTTACCGAGTCCGGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATAGCTTCCAGTGCGCCCTGTACATGTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                   <  1:1081158/150‑1 (MQ=255)

                                                                                                                                  cgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCATCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                   >  2:728484/1‑150 (MQ=255)

                                                                                                                                     aaaatgacccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaa                >  2:783651/8‑150 (MQ=255)

                                                                                                                                       gACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa              >  2:1293841/1‑150 (MQ=255)

                                                                                                                                       gACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa              <  1:1210660/150‑1 (MQ=255)

                                                                                                                                         cGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTTGAGAGCGTACGGCCAATCAAAAAc            <  1:1686778/150‑1 (MQ=255)

                                                                                                                                          gTCCCAGGCCACCGTTACCGAGGCCTGGGACATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACt           >  2:525677/1‑150 (MQ=255)

                                                                                                                                           acccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTc          >  2:375299/2‑150 (MQ=255)

                                                                                                                                            cccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc         >  2:1417567/1‑150 (MQ=255)

                                                                                                                                             ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCa        <  1:567831/150‑1 (MQ=255)

                                                                                                                                             ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCATCAACAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAATCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCa        <  1:1372042/150‑1 (MQ=255)

                                                                                                                                              cAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt       >  1:1088976/1‑150 (MQ=255)

                                                                                                                                              cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCCTCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGAATTAGGAGAGCGTACGGCCAATCAAAACCTCCag       >  2:282727/1‑149 (MQ=255)

                                                                                                                                              cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt       >  2:1209644/1‑150 (MQ=255)

                                                                                                                                              cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt       >  1:904067/1‑150 (MQ=255)

                                                                                                                                               cggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCGCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATc      <  2:1303303/149‑1 (MQ=255)

                                                                                                                                                ggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCg     <  1:560515/150‑1 (MQ=255)

                                                                                                                                                 gCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGa    >  2:493680/1‑150 (MQ=255)

                                                                                                                                                  ccACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGAc   >  2:1392764/1‑150 (MQ=255)

                                                                                                                                                   cACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa  >  1:45877/1‑150 (MQ=255)

CCAGCCAGTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACA  >  NC_000913/3566062‑3566358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`