BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,566,218	C→G	8.7%	G122R (GGT→CGT)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,218	0	C	G	8.7%	314.2 / 18.8	104	G122R (GGT→CGT)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base C (47/48); new base G (4/5); total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.18e-01

Only 100 of 109 total aligned reads displayed.

GTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAA  >  NC_000913/3566069‑3566366

gtagtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTAcc                                                                                                                                                      <  2:1669520/150‑1 (MQ=255)

  agtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCGAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGa                                                                                                                                                    >  2:1198360/1‑150 (MQ=255)

  agtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGATTCAAGGAAGCAAGCCGCCAGACGTCCCCGGCCACCGTTACCgc                                                                                                                                                    >  1:102511/1‑149 (MQ=255)

   gtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAg                                                                                                                                                   >  2:1325676/1‑150 (MQ=255)

   gtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAg                                                                                                                                                   <  1:614001/150‑1 (MQ=255)

    tCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAgg                                                                                                                                                  <  1:13902/150‑1 (MQ=255)

       ggCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCt                                                                                                                                               >  1:1636943/1‑150 (MQ=255)

         cGACTCTATCTGGCTACCGTTAACGATGATCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTgg                                                                                                                                             <  2:856076/150‑1 (MQ=255)

           aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATAGCGTATTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCTAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                           <  1:1279048/150‑2 (MQ=255)

           aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                           <  1:1223626/150‑2 (MQ=255)

           aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                           <  1:1709580/150‑2 (MQ=255)

           aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                           <  1:1703084/150‑2 (MQ=255)

           aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGt                                                                                                                                           <  2:528781/150‑1 (MQ=255)

              ctTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAt                                                                                                                                        >  1:653089/1‑150 (MQ=255)

                   tGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                   <  1:471182/150‑1 (MQ=255)

                   tGGCTACCGTTAACGATGGTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                   <  1:698891/150‑1 (MQ=255)

                     gCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                                 <  2:1594404/150‑1 (MQ=255)

                      cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTg                                                                                                                                              >  2:1324496/1‑136 (MQ=255)

                      cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtca                                                                                                                                >  2:546367/1‑150 (MQ=255)

                        aCCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcatc                                                                                                                              <  2:1273036/150‑1 (MQ=255)

                         ccGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGGCTGGGTCATTTTAGtcatca                                                                                                                             >  2:1523504/1‑150 (MQ=255)

                         ccGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcatca                                                                                                                             >  1:126056/1‑150 (MQ=255)

                           gTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTAACGAGGCCTGGGTCATTttcttaaaaaat                                                                                                                           >  2:1499144/1‑143 (MQ=255)

                            ttAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaatc                                                                                                                          <  1:888766/150‑1 (MQ=255)

                             tAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaatca                                                                                                                         <  1:546367/150‑1 (MQ=255)

                               aCGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGc                                                                                                                       <  2:213204/150‑1 (MQ=255)

                                   tGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCtt                                                                                                                   <  1:1325676/150‑1 (MQ=255)

                                     ttCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCg                                                                                                                 <  2:935920/150‑1 (MQ=255)

                                       cTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGTCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCGGGGTCATTTTCTTCGTCGATCAGCTCTTCgag                                                                                                               >  1:664106/1‑150 (MQ=255)

                                        tGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCgaga                                                                                                              <  1:105542/150‑1 (MQ=255)

                                         gCTTGAACATACCGTAGTCATAGCGGATGCCGAAACCGCGCCCCGGCAACCCTAACTTCGCCAGAGATTCAAGGAAGCAAGCCGCCAGACGTCCCAGCCCACCCTTCCCGAGGCCTGGGTCCATTTCTTCATCAAACCGCTCTTCgccaa                                                                                                             >  1:1446398/1‑146 (MQ=255)

                                            tGAACATACCGTAGTCATAGCGGAGGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTa                                                                                                          <  1:702440/150‑1 (MQ=255)

                                               aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAcc                                                                                                       <  1:1039068/150‑1 (MQ=255)

                                               aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAcc                                                                                                       <  2:1566810/150‑1 (MQ=255)

                                                cATACCGTTGTCATTGCGGATGCCGTATCCGCGCCGCGGCATCCCTAACGTCGCAAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAccc                                                                                                      <  1:843290/150‑1 (MQ=255)

                                                cATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAccc                                                                                                      >  2:567831/1‑150 (MQ=255)

                                                  tACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGCGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCa                                                                                                    >  2:1059306/1‑150 (MQ=255)

                                                       tAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCt                                                                                               <  1:650175/150‑1 (MQ=255)

                                                          tCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTcc                                                                                            <  1:952350/150‑1 (MQ=255)

                                                           cATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTATTCATCAATCAGCTCTTCGAGTTTTAACCCCATCGCTTCCa                                                                                           >  2:569220/1‑150 (MQ=255)

                                                             tAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCCGGCCCCCGGTGCCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAGTTAACCCCCTCGCTTCCAgg                                                                                         >  1:599783/1‑149 (MQ=255)

                                                              aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                        <  1:138459/150‑1 (MQ=255)

                                                              aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGGCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                        <  2:126056/150‑1 (MQ=255)

                                                              aGCGGATGACGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                        <  1:1499144/150‑1 (MQ=255)

                                                                cggcggCCGTCACCGCGCCCCGGCCACCCTAACGTCTCCAGAGAATCAACGAAGCACGCCGCCAGACGTCCCACGCCACCGTTACCGTGGCCTTGGTCATTTTCTTCCTCACTCAGCTCATCGAGATTTAACCCCATCGCTTCCAGTgcg                                                                                      <  2:121188/145‑1 (MQ=255)

                                                                cGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTgcg                                                                                      <  2:592026/150‑1 (MQ=255)

                                                                            ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCt                                                                          >  1:659275/1‑150 (MQ=255)

                                                                                gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGt                                                                      >  2:422461/1‑150 (MQ=255)

                                                                                gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGt                                                                      >  2:428745/1‑150 (MQ=255)

                                                                                   ccGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTaaa                                                                   >  2:202342/1‑150 (MQ=255)

                                                                                    cGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCACGCCACCCTTACCGCGGCCTGGGTCAATTAATACATCAATCAGCACTTCGAAAATTAACCCCATAGCGTCCACAGCCCCCTGTACATCTTCGTaaaa                                                                  >  2:1081158/1‑149 (MQ=255)

                                                                                       cAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACTTCCCAGGCCACCGTTACCGAGGCCTGGGGCAGTTTCGTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCATCCAGTGCGCCCTGTACAACTTCGTAAATTcg                                                               >  2:1047661/1‑149 (MQ=255)

                                                                                          cccTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAg                                                            <  1:849016/150‑1 (MQ=255)

                                                                                          cccTAACGTCGCCAGAGAATCAAGGAAGAAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTACATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAg                                                            >  2:1111186/1‑150 (MQ=255)

                                                                                              aaCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGac                                                        >  1:1218032/1‑150 (MQ=255)

                                                                                              aaCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGac                                                        <  1:1623750/150‑1 (MQ=255)

                                                                                               aCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGaca                                                       <  1:941605/150‑1 (MQ=255)

                                                                                                cGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTTCCGAGGCCTGGGTCATTTTCTTCATAAATCAGCTCGTCGAGATTTAACCCCATCGCTTACAGTGCGCCCTGTACCTCTTCGTAAATTCCTAGCGacca                                                      >  2:591480/1‑148 (MQ=255)

                                                                                                 gTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaac                                                     <  2:450302/150‑1 (MQ=255)

                                                                                                 gTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaac                                                     <  1:312417/150‑1 (MQ=255)

                                                                                                    gCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATg                                                  <  2:653089/150‑1 (MQ=255)

                                                                                                     ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATgg                                                 <  1:1143474/150‑1 (MQ=255)

                                                                                                     ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCCTTACCGCGGCCTGGGGCATTTTCGTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTTCGCCCGGTACAACGTCGTAAATTCCTAGCGACAACAAgg                                                 >  2:1590780/1‑150 (MQ=255)

                                                                                                      cAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGATGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGAGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGc                                                <  2:1335343/150‑1 (MQ=255)

                                                                                                        gagaATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGt                                              <  1:1059306/150‑1 (MQ=255)

                                                                                                           aaTCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                           >  1:188932/1‑150 (MQ=255)

                                                                                                              cAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCCTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgaga                                        >  2:1658828/1‑150 (MQ=255)

                                                                                                                aGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                                      <  1:408981/150‑1 (MQ=255)

                                                                                                                 ggAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGTGAGCg                                     >  1:62462/1‑150 (MQ=255)

                                                                                                                  gAAGCAAGCAGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCATCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGt                                    <  1:258281/150‑1 (MQ=255)

                                                                                                                     gcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTATTCAAAAATCACCTCTTCGAGATTTAACCCCAACGCTTCCAGTGCGCCCAGTACATCTTCGTAAATTACTAGCGACAACATCGCGTTGGAGAGCGTACg                                 >  2:1686778/1‑150 (MQ=255)

                                                                                                                       aagcCGCCAGACGTCCCAGGCCACCGTTAACGACGCCTGGGTCATTTTGTTCAACAATAAGCTATTAGAGATTTTAACCCATAGCTTACACTGAGCCCGGTTAAAATTAGTAAAATTATAGCGACAACAAGGAGGTTGGGAGCGTaaagc                               >  2:1422052/1‑146 (MQ=255)

                                                                                                                         gccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAGTTCCCCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAGTCCTAGCGACAACATGGCGGTGGCGAGCGTACGGCCa                             >  1:17985/1‑150 (MQ=255)

                                                                                                                           cgccAGTCGTCCCAGGCCACCGTTACCGAGTCCGGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATAGCTTCCAGTGCGCCCTGTACATGTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                           <  1:1081158/150‑1 (MQ=255)

                                                                                                                           cgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCATCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                           >  2:728484/1‑150 (MQ=255)

                                                                                                                              aaaatgacccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaa                        >  2:783651/8‑150 (MQ=255)

                                                                                                                                gACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      >  2:1293841/1‑150 (MQ=255)

                                                                                                                                gACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                      <  1:1210660/150‑1 (MQ=255)

                                                                                                                                  cGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTTGAGAGCGTACGGCCAATCAAAAAc                    <  1:1686778/150‑1 (MQ=255)

                                                                                                                                   gTCCCAGGCCACCGTTACCGAGGCCTGGGACATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACt                   >  2:525677/1‑150 (MQ=255)

                                                                                                                                    acccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTc                  >  2:375299/2‑150 (MQ=255)

                                                                                                                                     cccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                 >  2:1417567/1‑150 (MQ=255)

                                                                                                                                      ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCa                <  1:567831/150‑1 (MQ=255)

                                                                                                                                      ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCATCAACAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAATCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCa                <  1:1372042/150‑1 (MQ=255)

                                                                                                                                       cAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt               >  1:1088976/1‑150 (MQ=255)

                                                                                                                                       cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCCTCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGAATTAGGAGAGCGTACGGCCAATCAAAACCTCCag               >  2:282727/1‑149 (MQ=255)

                                                                                                                                       cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt               >  1:904067/1‑150 (MQ=255)

                                                                                                                                       cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt               >  2:1209644/1‑150 (MQ=255)

                                                                                                                                        cggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCGCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATc              <  2:1303303/149‑1 (MQ=255)

                                                                                                                                         ggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCg             <  1:560515/150‑1 (MQ=255)

                                                                                                                                          gCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGa            >  2:493680/1‑150 (MQ=255)

                                                                                                                                           ccACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGAc           >  2:1392764/1‑150 (MQ=255)

                                                                                                                                            cACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa          >  1:45877/1‑150 (MQ=255)

                                                                                                                                              ccGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAgg        >  1:608953/1‑150 (MQ=255)

                                                                                                                                                gTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgta      >  1:1442952/1‑150 (MQ=255)

                                                                                                                                                gTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgta      <  2:904067/150‑1 (MQ=255)

                                                                                                                                                    ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa  <  1:1523504/150‑1 (MQ=255)

                                                                                                                                                    ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa  >  2:777050/1‑150 (MQ=255)

                                                                                                                                                    ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTAGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa  >  1:614106/1‑150 (MQ=255)

                                                                                                                                                    ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAACCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa  >  1:1409021/1‑150 (MQ=255)

GTAGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAA  >  NC_000913/3566069‑3566366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`