BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,566,220	A→T	8.5%	L121H (CTC→CAC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,566,220	0	A	T	8.5%	250.4 / 7.9	106	L121H (CTC→CAC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand): ref base A (44/48); new base T (4/6); total (52/54)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACC  >  NC_000913/3566071‑3566369

agtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCGAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGa                                                                                                                                                       >  2:1198360/1‑150 (MQ=255)

 gtCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAg                                                                                                                                                      >  2:1325676/1‑150 (MQ=255)

  tCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAgg                                                                                                                                                     <  1:13902/150‑1 (MQ=255)

     ggCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCt                                                                                                                                                  >  1:1636943/1‑150 (MQ=255)

       cGACTCTATCTGGCTACCGTTAACGATGATCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTgg                                                                                                                                                <  2:856076/150‑1 (MQ=255)

         aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATAGCGTATTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCTAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                              <  1:1279048/150‑2 (MQ=255)

         aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                              <  1:1703084/150‑2 (MQ=255)

         aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCTCGGTAACGGTGGCCTggga                                                                                                                                              <  1:1223626/150‑2 (MQ=255)

         aCTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGt                                                                                                                                              <  2:528781/150‑1 (MQ=255)

          ctctTTCTGGCTACCGTTTACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGCGGCCTGGGTc                                                                                                                                             >  2:560515/1‑150 (MQ=255)

            ctTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCAt                                                                                                                                           >  1:653089/1‑150 (MQ=255)

                 tGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                      <  1:471182/150‑1 (MQ=255)

                 tGGCTACCGTTAACGATGGTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttct                                                                                                                                      <  1:698891/150‑1 (MQ=255)

                   gCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                                    >  2:1107968/1‑150 (MQ=255)

                   gCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTttcttc                                                                                                                                    <  1:548793/150‑1 (MQ=255)

                    cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTg                                                                                                                                                 <  1:1324496/136‑1 (MQ=255)

                    cTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTg                                                                                                                                                 >  2:1324496/1‑136 (MQ=255)

                      aCCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcatc                                                                                                                                 <  2:1273036/150‑1 (MQ=255)

                       ccGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGGCTGGGTCATTTTAGtcatca                                                                                                                                >  2:1523504/1‑150 (MQ=255)

                       ccGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTtcatca                                                                                                                                >  1:126056/1‑150 (MQ=255)

                         gTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTAACGAGGCCTGGGTCATTttcttaaaaaat                                                                                                                              >  2:1499144/1‑143 (MQ=255)

                           tAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCatcaatca                                                                                                                            <  1:546367/150‑1 (MQ=255)

                             aCGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGc                                                                                                                          <  2:213204/150‑1 (MQ=255)

                                 tGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCtt                                                                                                                      <  1:1325676/150‑1 (MQ=255)

                                   ttCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCg                                                                                                                    <  2:935920/150‑1 (MQ=255)

                                     cTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGTCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCGGGGTCATTTTCTTCGTCGATCAGCTCTTCgag                                                                                                                  >  1:664106/1‑150 (MQ=255)

                                      tGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCgaga                                                                                                                 <  1:105542/150‑1 (MQ=255)

                                       gCTTGAACATACCGTAGTCATAGCGGATGCCGAAACCGCGCCCCGGCAACCCTAACTTCGCCAGAGATTCAAGGAAGCAAGCCGCCAGACGTCCCAGCCCACCCTTCCCGAGGCCTGGGTCCATTTCTTCATCAAACCGCTCTTCgccaa                                                                                                                >  1:1446398/1‑146 (MQ=255)

                                          tGAACATACCGTAGTCATAGCGGAGGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTa                                                                                                             <  1:702440/150‑1 (MQ=255)

                                            aaCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGAAATCCGCCAGACGTCCGAGGCCTCGGGTACCGAGGGCTGGGTGATTTTATTCATAAATCAGGTCTTCGAGATTTTAc                                                                                                           >  2:1372042/1‑150 (MQ=255)

                                             aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAcc                                                                                                          <  1:1039068/150‑1 (MQ=255)

                                             aCATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAcc                                                                                                          <  2:1566810/150‑1 (MQ=255)

                                              cATACCGTTGTCATTGCGGATGCCGTATCCGCGCCGCGGCATCCCTAACGTCGCAAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAccc                                                                                                         <  1:843290/150‑1 (MQ=255)

                                              cATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAccc                                                                                                         >  2:567831/1‑150 (MQ=255)

                                                tACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGCGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCa                                                                                                       >  2:1059306/1‑150 (MQ=255)

                                                     tAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCt                                                                                                  <  1:650175/150‑1 (MQ=255)

                                                        tCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTcc                                                                                               <  1:952350/150‑1 (MQ=255)

                                                         cATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCa                                                                                              <  1:1658682/150‑1 (MQ=255)

                                                         cATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTATTCATCAATCAGCTCTTCGAGTTTTAACCCCATCGCTTCCa                                                                                              >  2:569220/1‑150 (MQ=255)

                                                           tAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCCGGCCCCCGGTGCCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAGTTAACCCCCTCGCTTCCAgg                                                                                            >  1:599783/1‑149 (MQ=255)

                                                            aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                           <  1:138459/150‑1 (MQ=255)

                                                            aGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGGCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                           <  2:126056/150‑1 (MQ=255)

                                                            aGCGGATGACGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTg                                                                                           <  1:1499144/150‑1 (MQ=255)

                                                              cggcggCCGTCACCGCGCCCCGGCCACCCTAACGTCTCCAGAGAATCAACGAAGCACGCCGCCAGACGTCCCACGCCACCGTTACCGTGGCCTTGGTCATTTTCTTCCTCACTCAGCTCATCGAGATTTAACCCCATCGCTTCCAGTgcg                                                                                         <  2:121188/145‑1 (MQ=255)

                                                              cGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTgcg                                                                                         <  2:592026/150‑1 (MQ=255)

                                                                          ccGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCCCCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCt                                                                             >  1:659275/1‑150 (MQ=255)

                                                                           cgcgCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCGCTTCGAGAGTTAACCCCATCGCTTCCAGGGCGCCCTGGACATcgt                                                                            >  1:667805/1‑148 (MQ=255)

                                                                              gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGt                                                                         >  2:422461/1‑150 (MQ=255)

                                                                              gcCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGt                                                                         >  2:428745/1‑150 (MQ=255)

                                                                                 ccGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTaaa                                                                      >  2:202342/1‑150 (MQ=255)

                                                                                  cGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCACGCCACCCTTACCGCGGCCTGGGTCAATTAATACATCAATCAGCACTTCGAAAATTAACCCCATAGCGTCCACAGCCCCCTGTACATCTTCGTaaaa                                                                     >  2:1081158/1‑149 (MQ=255)

                                                                                     cAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACTTCCCAGGCCACCGTTACCGAGGCCTGGGGCAGTTTCGTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCATCCAGTGCGCCCTGTACAACTTCGTAAATTcg                                                                  >  2:1047661/1‑149 (MQ=255)

                                                                                        cccTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAg                                                               <  1:849016/150‑1 (MQ=255)

                                                                                        cccTAACGTCGCCAGAGAATCAAGGAAGAAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTACATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAg                                                               >  2:1111186/1‑150 (MQ=255)

                                                                                            aaCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGac                                                           <  1:1623750/150‑1 (MQ=255)

                                                                                            aaCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGac                                                           >  1:1218032/1‑150 (MQ=255)

                                                                                             aCGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGaca                                                          <  1:941605/150‑1 (MQ=255)

                                                                                              cGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTTCCGAGGCCTGGGTCATTTTCTTCATAAATCAGCTCGTCGAGATTTAACCCCATCGCTTACAGTGCGCCCTGTACCTCTTCGTAAATTCCTAGCGacca                                                         >  2:591480/1‑148 (MQ=255)

                                                                                               gTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaac                                                        <  2:450302/150‑1 (MQ=255)

                                                                                               gTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGacaac                                                        <  1:312417/150‑1 (MQ=255)

                                                                                                  gCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATg                                                     <  2:653089/150‑1 (MQ=255)

                                                                                                   ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATgg                                                    <  1:1143474/150‑1 (MQ=255)

                                                                                                   ccAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCCTTACCGCGGCCTGGGGCATTTTCGTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTTCGCCCGGTACAACGTCGTAAATTCCTAGCGACAACAAgg                                                    >  2:1590780/1‑150 (MQ=255)

                                                                                                    cAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGATGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGAGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGc                                                   <  2:1335343/150‑1 (MQ=255)

                                                                                                      gagaATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGt                                                 <  1:1059306/150‑1 (MQ=255)

                                                                                                         aaTCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTgg                                              >  1:188932/1‑150 (MQ=255)

                                                                                                            cAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCCTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGgaga                                           >  2:1658828/1‑150 (MQ=255)

                                                                                                              aGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                                         <  2:188932/150‑1 (MQ=255)

                                                                                                              aGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGc                                         <  1:408981/150‑1 (MQ=255)

                                                                                                               ggAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGTGAGCg                                        >  1:62462/1‑150 (MQ=255)

                                                                                                                gAAGCAAGCAGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCATCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGt                                       <  1:258281/150‑1 (MQ=255)

                                                                                                                   gcaagcCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTATTCAAAAATCACCTCTTCGAGATTTAACCCCAACGCTTCCAGTGCGCCCAGTACATCTTCGTAAATTACTAGCGACAACATCGCGTTGGAGAGCGTACg                                    >  2:1686778/1‑150 (MQ=255)

                                                                                                                     aagcCGCCAGACGTCCCAGGCCACCGTTAACGACGCCTGGGTCATTTTGTTCAACAATAAGCTATTAGAGATTTTAACCCATAGCTTACACTGAGCCCGGTTAAAATTAGTAAAATTATAGCGACAACAAGGAGGTTGGGAGCGTaaagc                                  >  2:1422052/1‑146 (MQ=255)

                                                                                                                       gccgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGAGTTCCCCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAAGTCCTAGCGACAACATGGCGGTGGCGAGCGTACGGCCa                                >  1:17985/1‑150 (MQ=255)

                                                                                                                         cgccAGTCGTCCCAGGCCACCGTTACCGAGTCCGGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATAGCTTCCAGTGCGCCCTGTACATGTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                              <  1:1081158/150‑1 (MQ=255)

                                                                                                                         cgccAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCATCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAAt                              >  2:728484/1‑150 (MQ=255)

                                                                                                                            aaaatgacccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaa                           >  2:783651/8‑150 (MQ=255)

                                                                                                                              gACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCaaaa                         <  1:1210660/150‑1 (MQ=255)

                                                                                                                                cGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTTGAGAGCGTACGGCCAATCAAAAAc                       <  1:1686778/150‑1 (MQ=255)

                                                                                                                                 gTCCCAGGCCACCGTTACCGAGGCCTGGGACATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACt                      >  2:525677/1‑150 (MQ=255)

                                                                                                                                  acccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTc                     >  2:375299/2‑150 (MQ=255)

                                                                                                                                   cccAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTcc                    >  2:1417567/1‑150 (MQ=255)

                                                                                                                                    ccAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCa                   <  1:567831/150‑1 (MQ=255)

                                                                                                                                     cAGGCCTCGGTAACGGTGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt                  >  1:1088976/1‑150 (MQ=255)

                                                                                                                                     cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCCTCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGAATTAGGAGAGCGTACGGCCAATCAAAACCTCCag                  >  2:282727/1‑149 (MQ=255)

                                                                                                                                     cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt                  >  2:1209644/1‑150 (MQ=255)

                                                                                                                                     cAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCAt                  >  1:904067/1‑150 (MQ=255)

                                                                                                                                      cggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCGCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATc                 <  2:1303303/149‑1 (MQ=255)

                                                                                                                                       ggCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCg                <  1:560515/150‑1 (MQ=255)

                                                                                                                                        gCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGa               >  2:493680/1‑150 (MQ=255)

                                                                                                                                         ccACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGAc              >  2:1392764/1‑150 (MQ=255)

                                                                                                                                          cACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACa             >  1:45877/1‑150 (MQ=255)

                                                                                                                                            ccGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAgg           >  1:608953/1‑150 (MQ=255)

                                                                                                                                              gTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgta         >  1:1442952/1‑150 (MQ=255)

                                                                                                                                              gTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGgta         <  2:904067/150‑1 (MQ=255)

                                                                                                                                                  ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa     <  1:1523504/150‑1 (MQ=255)

                                                                                                                                                  ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa     >  2:777050/1‑150 (MQ=255)

                                                                                                                                                  ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTAGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa     >  1:614106/1‑150 (MQ=255)

                                                                                                                                                  ccGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAAACCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTaa     >  1:1409021/1‑150 (MQ=255)

                                                                                                                                                     aGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAAcc  <  1:728484/150‑1 (MQ=255)

AGTCTGGCGACTCTTTCTGGCTACCGTTAACGATGTTCTGCTTGAACATACCGTAGTCATAGCGGATGCCGTAACCGCGCCCCGGCAACCCTAACGTCGCCAGAGAATCAAGGAAGCAAGCCGCCAGACGTCCCAGGCCACCGTTACCGAGGCCTGGGTCATTTTCTTCATCAATCAGCTCTTCGAGATTTAACCCCATCGCTTCCAGTGCGCCCTGTACATCTTCGTAAATTCCTAGCGACAACATGGCGTTGGAGAGCGTACGGCCAATCAAAAACTCCATCGACAGGTAGTAAACC  >  NC_000913/3566071‑3566369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`