Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,570,719 C→G 5.3% V201L (GTG→CTG)  glgX ← glycogen debranching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,570,7190CG 5.3% 381.5 / 9.5 113V201L (GTG→CTG) glgXglycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base C (46/61);  new base G (2/4);  total (48/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01

Only 100 of 113 total aligned reads displayed.

TTTGATTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGA  >  NC_000913/3570573‑3570865
                                                                                                                                                   |                                                                                                                                                  
tttGATTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTgg                                                                                                                                                 <  2:1041501/150‑1 (MQ=255)
 ttGATTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGgc                                                                                                                                                <  2:1360902/150‑1 (MQ=255)
    aTTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGgcagc                                                                                                                                             <  1:952748/150‑1 (MQ=255)
    aTTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGgcagc                                                                                                                                             >  2:1200014/1‑150 (MQ=255)
    aTTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGACGGATGCAGCGCAATCTTCGCCACCGGGTTGTAACCCCAGTAGTTTCTTAGCCCCTTGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGgcagc                                                                                                                                             <  1:228122/150‑1 (MQ=255)
       gCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGt                                                                                                                                          >  2:354512/1‑150 (MQ=255)
         aTCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTc                                                                                                                                        >  1:1560150/1‑150 (MQ=255)
          tCGCGAAACTCATCCAGCGCCTTTTCTGGCGAGCTGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAAGCCAAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTcc                                                                                                                                       <  2:437997/150‑1 (MQ=255)
          tCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCTGCCAGTGGCGCAGtttg                                                                                                                                       <  1:1073305/150‑3 (MQ=255)
          tCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTcc                                                                                                                                       <  1:520066/150‑1 (MQ=255)
           cgcgAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCTGCCAGTGGCGCAGtttgc                                                                                                                                      <  1:692464/150‑4 (MQ=255)
           cgcgAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCTCCACTGGCAGCAGTTCCa                                                                                                                                      >  2:1484894/1‑150 (MQ=255)
           cgcgAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCAGGCGTTGCAGACGTGGTTCACTGGCAAACTGCTGCCAGTGGCGCAGtttgc                                                                                                                                      <  1:1548870/150‑4 (MQ=255)
            gcgAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAg                                                                                                                                     <  1:1496201/150‑1 (MQ=255)
            gcgAAACTCATCCAGCGCCGTTTCTGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAgc                                                                                                                                    >  1:1634681/1‑150 (MQ=255)
                aaCTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGcgcg                                                                                                                                 >  2:541614/1‑150 (MQ=255)
                aaCTCATCAAGCGCCGTTTCTGCGAGCAGGCATACGCCGGATGCAGCGGAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCACATGCGTTGCAGACGTGGTTCACAGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCgg                                                                                                                                >  2:934176/1‑150 (MQ=255)
                        cAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGcc                                                                                                                         >  2:77493/1‑150 (MQ=255)
                        cAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGCTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGcc                                                                                                                         <  1:1105479/150‑1 (MQ=255)
                           cgcCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCaa                                                                                                                      >  2:1047059/1‑150 (MQ=255)
                              cGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTg                                                                                                                   >  1:183125/1‑150 (MQ=255)
                                  tCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTc                                                                                                               <  1:618236/150‑1 (MQ=255)
                                   cTGGCGAGCAGGCATACGCCGGATGCAGCGCCAACATCGCCACCGGGTTGTAACCCCAGTAGTTACATAGCCCCAAGCGTTGCAGACGTGGTTCACTGGCCAACTGCGCCACTGGCAGAAGGGCCCGCGCGGTAATGGCCAATGGTTTCa                                                                                                              >  1:1197149/1‑150 (MQ=255)
                                   cTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCa                                                                                                              <  2:646833/150‑1 (MQ=255)
                                     ggCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCaaa                                                                                                            >  1:451340/1‑150 (MQ=255)
                                     ggCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCaaa                                                                                                            >  1:1677864/1‑150 (MQ=255)
                                      gCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGAGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAAt                                                                                                           <  1:1047059/150‑1 (MQ=255)
                                       cGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATa                                                                                                          <  2:471437/150‑1 (MQ=255)
                                        gAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAg                                                                                                         <  2:1692999/150‑1 (MQ=255)
                                         aGCAGGCATACGCCGGATGCAGCGCAGACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGt                                                                                                        >  2:275327/1‑150 (MQ=255)
                                          gCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTGACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGtt                                                                                                       <  2:128405/150‑1 (MQ=255)
                                          gCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGtt                                                                                                       <  2:1214332/150‑1 (MQ=255)
                                            aGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGa                                                                                                     <  2:26406/150‑1 (MQ=255)
                                             ggCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGat                                                                                                    >  2:1477229/1‑150 (MQ=255)
                                              gCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGatc                                                                                                   <  1:1314651/150‑1 (MQ=255)
                                               cATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGatca                                                                                                  <  1:1168215/150‑1 (MQ=255)
                                                aTACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGatcat                                                                                                 >  1:571949/1‑150 (MQ=255)
                                                 tACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGatcatc                                                                                                <  1:1015317/150‑1 (MQ=255)
                                                    gCCGGATGCAGCGCAAACATCGACACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCAcc                                                                                             <  2:102922/150‑1 (MQ=255)
                                                     ccGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCg                                                                                            <  2:1232309/150‑1 (MQ=255)
                                                       ggATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGa                                                                                          <  2:828552/150‑1 (MQ=255)
                                                       ggATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGa                                                                                          >  2:1616813/1‑150 (MQ=255)
                                                        gATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGAt                                                                                         <  1:1646724/150‑1 (MQ=255)
                                                                 cAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAggg                                                                                <  1:628084/150‑1 (MQ=255)
                                                                 cAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAggg                                                                                <  1:270411/150‑1 (MQ=255)
                                                                       tCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGGGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCGTtat                                                                          >  1:173815/1‑150 (MQ=255)
                                                                        cGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTtata                                                                         <  1:984391/150‑1 (MQ=255)
                                                                         gCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATaa                                                                        >  2:756951/1‑150 (MQ=255)
                                                                          ccACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAg                                                                       <  1:227159/150‑1 (MQ=255)
                                                                           cACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGAGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGt                                                                      <  2:93702/150‑1 (MQ=255)
                                                                              cGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGcc                                                                   <  1:1616813/150‑1 (MQ=255)
                                                                                   tGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGa                                                              >  2:436256/1‑150 (MQ=255)
                                                                                   tGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGa                                                              >  1:776421/1‑150 (MQ=255)
                                                                                   tGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGa                                                              <  1:275327/150‑1 (MQ=255)
                                                                                      aaCCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGAtct                                                           >  1:1231501/1‑150 (MQ=255)
                                                                                      aaCCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGAtct                                                           <  2:178090/150‑1 (MQ=255)
                                                                                        ccccAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCg                                                         >  2:315370/1‑150 (MQ=255)
                                                                                         cccAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCTGCAGTTCCAGCGCGGTAATGCCAAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGa                                                        <  2:938102/150‑1 (MQ=255)
                                                                                         cccAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGa                                                        <  2:501069/150‑1 (MQ=255)
                                                                                               agtTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTACAGCGCTGTAATGCCAAATTGTTTCAAATATTTTATCATCACCGGATGCACGAGGGCTTTATAATTGCCACGTATCTAGACCGCGa                                                  >  1:1061248/1‑150 (MQ=255)
                                                                                                  tACTTAGCCCCATGCGTTGCAGACGTGGTTAACTGGCAAACTGCTCCACTGGCAGCATTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGAGCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCGCGACCGGGAtcg                                               >  2:281564/1‑149 (MQ=255)
                                                                                                    cTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATTGTTGATCATCACCGGATGCCCGAGGTCTTTATAAGTGCCACGGATCTCGACCGGGATCTcc                                             <  1:989716/150‑1 (MQ=255)
                                                                                                    cTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTcc                                             >  2:1023102/1‑150 (MQ=255)
                                                                                                      tAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCgg                                           >  2:1564314/1‑150 (MQ=255)
                                                                                                      tAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCAACTGGCAGCAGTTCCATCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCgg                                           >  1:1131574/1‑150 (MQ=255)
                                                                                                       cgCCCCATGCGATGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCACTTGCTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCggg                                          <  1:224761/149‑1 (MQ=255)
                                                                                                       aGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCggg                                          >  1:1715109/1‑150 (MQ=255)
                                                                                                        gCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGt                                         <  1:756951/150‑1 (MQ=255)
                                                                                                                cGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTAc                                 >  2:1156474/1‑150 (MQ=255)
                                                                                                                 gTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACg                                >  2:1593424/1‑150 (MQ=255)
                                                                                                                    gCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTa                             <  2:1560150/150‑1 (MQ=255)
                                                                                                                      aGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAAt                           <  2:183125/150‑1 (MQ=255)
                                                                                                                        aCGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATcc                         <  2:1392164/150‑1 (MQ=255)
                                                                                                                        aCGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATcc                         >  1:1688360/1‑150 (MQ=255)
                                                                                                                          gTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATGGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCtt                       <  2:1139579/150‑1 (MQ=255)
                                                                                                                          gTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGGAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGACACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCtt                       >  2:1108578/1‑150 (MQ=255)
                                                                                                                           tGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCttt                      <  1:937324/150‑1 (MQ=255)
                                                                                                                            ggTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGGAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTg                     <  1:411535/150‑1 (MQ=255)
                                                                                                                             gTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGa                    <  2:1324906/150‑1 (MQ=255)
                                                                                                                             gTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGa                    >  1:487216/1‑150 (MQ=255)
                                                                                                                                  cTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGa                    <  1:256112/145‑1 (MQ=255)
                                                                                                                                  cTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGa                    >  2:256112/1‑145 (MQ=255)
                                                                                                                                   gctggaaCTGCTGCCAGTGGCGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGCCATggg              >  1:1092759/6‑150 (MQ=255)
                                                                                                                                    ggCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATTCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCt            <  1:541614/150‑1 (MQ=255)
                                                                                                                                    ggCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGc             >  2:1141501/1‑150 (MQ=255)
                                                                                                                                    ggCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGc             <  1:763470/150‑1 (MQ=255)
                                                                                                                                     gCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCt            <  1:960594/150‑1 (MQ=255)
                                                                                                                                      cAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCtt           <  2:822201/150‑1 (MQ=255)
                                                                                                                                       aaaCTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTc          <  2:1509761/150‑1 (MQ=255)
                                                                                                                                        aaCTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCa         >  1:1522299/1‑150 (MQ=255)
                                                                                                                                         actgctgcCAGTGGCGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCAt        >  2:97706/7‑150 (MQ=255)
                                                                                                                                         aCTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCAt        <  1:760571/150‑1 (MQ=255)
                                                                                                                                         aCTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCAt        >  1:452561/1‑150 (MQ=255)
                                                                                                                                         aCTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCAt        <  1:1484894/150‑1 (MQ=255)
                                                                                                                                           tGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTTATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCCCGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATaa      >  1:1245381/1‑150 (MQ=255)
                                                                                                                                           tGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATaa      <  2:1029904/150‑1 (MQ=255)
                                                                                                                                            gcgcCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATaaa     <  1:929265/150‑1 (MQ=255)
                                                                                                                                             cgcCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAat    <  1:1156474/150‑1 (MQ=255)
                                                                                                                                                ccACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAatga  <  2:1121246/150‑1 (MQ=255)
                                                                                                                                                ccACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAatga  >  1:1149263/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTTGATTGCATCGCGAAACTCATCCAGCGCCGTTTCTGGCGAGCAGGCATACGCCGGATGCAGCGCAAACATCGCCACCGGGTTGTAACCCCAGTAGTTACTTAGCCCCATGCGTTGCAGACGTGGTTCACTGGCAAACTGCGCCACTGGCAGCAGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGA  >  NC_000913/3570573‑3570865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: