Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,614,203 A→G 5.1% K179K (AAA→AAG nikA → nickel/heme ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,614,2030AG 5.1% 348.6 / 6.4 117K179K (AAA→AAGnikAnickel/heme ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (57/53);  new base G (3/3);  total (61/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01

Only 100 of 122 total aligned reads displayed.

GTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGT  >  NC_000913/3614054‑3614352
                                                                                                                                                     |                                                                                                                                                     
gttgATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTaaa                                                                                                                                                       <  1:498164/150‑1 (MQ=255)
gttgATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTaaa                                                                                                                                                       >  1:1196901/1‑150 (MQ=255)
 ttgATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAg                                                                                                                                                      <  1:245089/150‑1 (MQ=255)
  tgATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAACGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAgc                                                                                                                                                     >  1:1466949/1‑150 (MQ=255)
  tgATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAgc                                                                                                                                                     >  1:1543526/1‑150 (MQ=255)
   gATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAgcg                                                                                                                                                    <  2:204077/150‑1 (MQ=255)
   gATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAgcg                                                                                                                                                    <  1:1374092/150‑1 (MQ=255)
     tGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAACCCATGAAACCATGAACGGAATTAAAGCGcc                                                                                                                                                  >  2:258680/1‑150 (MQ=255)
     tGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTAACCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATTAAACAATGAACGGAATTAAAGCGcc                                                                                                                                                  >  1:291977/1‑150 (MQ=255)
        tAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGAt                                                                                                                                               >  1:504160/1‑150 (MQ=255)
             cACTCCGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCAACTAACATTTCCTGCAATAACTGTCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAACCCATGAACGGAATTAAAGCGCCGATTGGCa                                                                                                                                          <  2:620690/150‑1 (MQ=255)
             cACTCAGTAAAACAGAGCTGCAAATTTCCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCa                                                                                                                                          <  2:614622/150‑1 (MQ=255)
                  aGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGa                                                                                                                                     >  2:1149461/1‑150 (MQ=255)
                   gTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGAc                                                                                                                                    >  1:648642/1‑150 (MQ=255)
                       aaCAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTg                                                                                                                                <  1:905965/150‑1 (MQ=255)
                       aaCAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTg                                                                                                                                <  1:1484128/150‑1 (MQ=255)
                         cAGAGCTGCAAATTACCCTGAAAAGCGCCGACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGa                                                                                                                              >  2:1178792/1‑150 (MQ=255)
                             gCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTAAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGAtttt                                                                                                                          >  1:699844/1‑150 (MQ=255)
                              cTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAACAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTg                                                                                                                         >  1:701394/1‑150 (MQ=255)
                              cTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTg                                                                                                                         <  1:1150555/150‑1 (MQ=255)
                               tGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGc                                                                                                                        <  1:202939/150‑1 (MQ=255)
                               ggCAAATTTCCCTGAAAAGGGGCTACTTTCCTTTCCTGCAAGAACTGGCCCGGCCCCGGCCTTTCCCGTTTATCGCTCCCTCGGAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGc                                                                                                                        <  1:802558/149‑1 (MQ=255)
                                gCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCa                                                                                                                       >  2:1688363/1‑150 (MQ=255)
                                 gaaaTTACGCTGAAAAGCGCCTACTATCCTTTCCTGCAATAACTGGCCCTGCCCGGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACGGGACCGTGGATTTTGCAg                                                                                                                      <  2:906324/149‑1 (MQ=255)
                                 cAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAg                                                                                                                      <  2:941108/150‑1 (MQ=255)
                                 cAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAg                                                                                                                      <  2:1154552/150‑1 (MQ=255)
                                        cccTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGa                                                                                                               <  2:440928/150‑1 (MQ=255)
                                          cTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGaaa                                                                                                             >  2:1301755/1‑150 (MQ=255)
                                          cTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGaaa                                                                                                             >  2:1308494/1‑150 (MQ=255)
                                           tGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCAGACCGTGGATTTTGCAGGAATCGAAAc                                                                                                            <  2:1196901/150‑1 (MQ=255)
                                           tGAAAAGATCATACTATCCTTTACTGCAAGAACTGGCCATGCGCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAAc                                                                                                            <  2:1136998/150‑1 (MQ=255)
                                            gAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCGGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACt                                                                                                           <  2:1102896/150‑1 (MQ=255)
                                             aaaaGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTg                                                                                                          <  1:1566953/150‑1 (MQ=255)
                                               aaGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGaa                                                                                                        <  1:1149461/150‑1 (MQ=255)
                                                  cgcCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCAGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGCACGGATTTAACGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGACACTGAATCa                                                                                                     >  1:74452/1‑150 (MQ=255)
                                                      tactaTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTAc                                                                                                 >  1:1208005/1‑150 (MQ=255)
                                                       actaTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACg                                                                                                <  2:1466949/150‑1 (MQ=255)
                                                       actaTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACg                                                                                                <  2:1543526/150‑1 (MQ=255)
                                                          aTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATg                                                                                             <  1:389204/150‑1 (MQ=255)
                                                          aTCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGAGTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGAtt                                                                                             >  1:632269/1‑149 (MQ=255)
                                                           tCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCACTTTAAAAACCATGAAACCATGAAAGGAAATAAAGCGCCGATGGGCACCGGACCGTGGATTTTGCAGGAATCTAAACTGAATCAGTAAGAtct                                                                                            >  2:41548/1‑148 (MQ=255)
                                                             cTTTCCTGCAAGAACTGTCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAATAACCATGAAACCATGAACGGAATTATAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCt                                                                                          <  1:519435/150‑1 (MQ=255)
                                                             cTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCt                                                                                          >  2:389644/1‑150 (MQ=255)
                                                             cTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCt                                                                                          >  1:893555/1‑150 (MQ=255)
                                                              tttCCTGCTAGAACTTTACCTGCCACGTCGTTTCATTTTTATCGCTCGGTGGCTGTTTAATAATCTTGTTACGATGATCTTAATTAAAGCGACTATTGTCAGCGTTCCGTGGATTTTGCAGGAATCGAAACGGAATCTGTACTATGtata                                                                                         <  2:1568533/150‑4 (MQ=255)
                                                                tCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTAAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCg                                                                                       <  2:699844/150‑1 (MQ=255)
                                                                 ccTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGt                                                                                      >  2:562052/1‑150 (MQ=255)
                                                                  cTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTc                                                                                     >  2:429600/1‑150 (MQ=255)
                                                                   tGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTcc                                                                                    >  1:1654535/1‑150 (MQ=255)
                                                                    gCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAACCCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATCTCTTCGTCCg                                                                                   >  2:243959/1‑150 (MQ=255)
                                                                     cAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGt                                                                                  >  2:690906/1‑150 (MQ=255)
                                                                       aGAACTGGCCCTGCCCCGTCCTTTCCGGTTTATCGCTCCCTCGCAGTTTAAAAACGATGAAACCATGATCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAATCTGAATCAGTACGATGTCTGCGTCCGTaa                                                                                >  1:278731/1‑150 (MQ=255)
                                                                        gAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAAc                                                                               <  2:382751/150‑1 (MQ=255)
                                                                        gAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAAc                                                                               >  1:1476750/1‑150 (MQ=255)
                                                                          aCTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGa                                                                             >  1:1179703/1‑150 (MQ=255)
                                                                          aCTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGa                                                                             >  2:870309/1‑150 (MQ=255)
                                                                               cccTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAact                                                                        >  1:412082/1‑150 (MQ=255)
                                                                                  tGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAactact                                                                     >  2:383195/1‑150 (MQ=255)
                                                                                   gCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTg                                                                    >  1:217926/1‑150 (MQ=255)
                                                                                    ccccGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAGGCGCCGATTGGCACCGGTCCGTGGATTTTGCAGGAATCGAATCTGAATCAGTAGGATGTCTTCGTCCGTAACGAATACTTCTgg                                                                   >  2:689375/1‑150 (MQ=255)
                                                                                    ccccGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAGGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTgg                                                                   >  2:695359/1‑150 (MQ=255)
                                                                                    ccccGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTgg                                                                   >  2:421225/1‑150 (MQ=255)
                                                                                    ccccGTCCTTTCAGTTTAATCGCTCCCTCGCCGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTgg                                                                   <  2:1043576/150‑1 (MQ=255)
                                                                                     cccGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTggg                                                                  >  2:1698427/1‑150 (MQ=255)
                                                                                       cGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAACCCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGc                                                                <  1:258680/150‑1 (MQ=255)
                                                                                         gccTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGa                                                              <  1:1178792/149‑1 (MQ=255)
                                                                                           cTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCTGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGaaa                                                            >  1:844378/1‑150 (MQ=255)
                                                                                           cTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGaaa                                                            >  1:1594279/1‑150 (MQ=255)
                                                                                              tCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGc                                                         >  1:603121/1‑150 (MQ=255)
                                                                                                 gTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAg                                                      <  2:217926/150‑1 (MQ=255)
                                                                                                     tATCGCTGCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACGGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTGTTCGTCCGTAACGAAAACTTCTGGGGCGATAAGCAAGCGAt                                                  >  1:46746/1‑150 (MQ=255)
                                                                                                     tATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGAt                                                  >  2:1219629/1‑150 (MQ=255)
                                                                                                      aTCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGATTTAAAGCGCCGATTGGCACCGGACCTTGGATTTTGAAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAAATACTGGGGCGAAATGCCAGCGAtt                                                 >  1:727683/1‑150 (MQ=255)
                                                                                                       tCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTa                                                <  1:1688363/150‑1 (MQ=255)
                                                                                                         gCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTaaa                                              >  2:550181/1‑150 (MQ=255)
                                                                                                              cTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGAt                                         >  1:769344/1‑150 (MQ=255)
                                                                                                                  cAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCAcc                                     <  2:1208005/150‑1 (MQ=255)
                                                                                                                     tttAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCttt                                  >  2:908190/1‑150 (MQ=255)
                                                                                                                         aaaaCCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACg                              <  2:1411821/150‑1 (MQ=255)
                                                                                                                         aaaaCCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACg                              <  2:1109201/150‑1 (MQ=255)
                                                                                                                           aaccatgaaaccatgaAAGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGAACGATGTCTTCGTCCGTACCGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACATTTAACGTc                            >  1:137510/1‑150 (MQ=255)
                                                                                                                            accatgaaaccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTTCAGGAATCGAAAAAGAATAAATACGATGTCTTCGTCCGAAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTAAACGtaa                           >  1:1044601/1‑148 (MQ=255)
                                                                                                                             ccatgaaaccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCAt                          <  1:1301755/150‑1 (MQ=255)
                                                                                                                              catgaaaccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATc                         >  2:1239632/1‑150 (MQ=255)
                                                                                                                              catgaaaccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATc                         <  2:221126/150‑1 (MQ=255)
                                                                                                                              aatctctccatgaaCTGAATTACAGCGGCTACTGGCACCGGACCGTGGATTTTGCACGAATCTATACTGAATCAGTACGATGGCTTCGTCCGGAACGAAAACTACTGTGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATc                         <  2:1452833/143‑1 (MQ=255)
                                                                                                                                tgaaaccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATccc                       >  1:1348614/1‑150 (MQ=255)
                                                                                                                                   aaccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGa                    >  1:573208/1‑150 (MQ=255)
                                                                                                                                     ccatgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGAcc                  >  1:1350360/1‑150 (MQ=255)
                                                                                                                                       atgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCg                <  1:193411/150‑1 (MQ=255)
                                                                                                                                        tgaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGa               >  1:1211465/1‑150 (MQ=255)
                                                                                                                                         gaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGAc              <  2:412082/150‑1 (MQ=255)
                                                                                                                                         gaaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGGCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGAc              <  2:40896/150‑1 (MQ=255)
                                                                                                                                          aaCGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACt             >  1:772618/1‑150 (MQ=255)
                                                                                                                                            cGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGATACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTaa           >  1:1300917/1‑149 (MQ=255)
                                                                                                                                             ggAATTAAGGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTAcc          <  1:695359/150‑1 (MQ=255)
                                                                                                                                             ggAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTAcc          <  2:769344/150‑1 (MQ=255)
                                                                                                                                                   aaaGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCAGACTACCcgcgcg    >  1:389525/1‑150 (MQ=255)
                                                                                                                                                     aGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGt  >  1:1651800/1‑150 (MQ=255)
                                                                                                                                                     aGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTAGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGCCCCGCCTACCCGCGCgga  >  2:103690/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTGATGTTAAAGCACTCAGTAAAACAGAGCTGCAAATTACCCTGAAAAGCGCCTACTATCCTTTCCTGCAAGAACTGGCCCTGCCCCGTCCTTTCCGTTTTATCGCTCCCTCGCAGTTTAAAAACCATGAAACCATGAACGGAATTAAAGCGCCGATTGGCACCGGACCGTGGATTTTGCAGGAATCGAAACTGAATCAGTACGATGTCTTCGTCCGTAACGAAAACTACTGGGGCGAAAAGCCAGCGATTAAAAAGATCACCTTTAACGTCATCCCGGACCCGACTACCCGCGCGGT  >  NC_000913/3614054‑3614352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: