Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,616,399 T→G 6.0% V73G (GTG→GGG)  nikC → nickel ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,616,3990TG 6.0% 260.1 / 3.1 102V73G (GTG→GGG) nikCnickel ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (41/52);  new base G (5/2);  total (48/54)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTG  >  NC_000913/3616252‑3616547
                                                                                                                                                   |                                                                                                                                                    
ttGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCCCCGATCACTTAGGTCGCGCTATTTTCTCGCGGCTGATGGCAGCGACCCGCgtgt                                                                                                                                                    >  2:1339621/1‑150 (MQ=255)
  gcgcTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCg                                                                                                                                                  >  1:1216599/1‑150 (MQ=255)
   cgcTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCCCTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGc                                                                                                                                                 >  1:659266/1‑150 (MQ=255)
     cTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGAGCAGCACTGGCGGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtc                                                                                                                                               <  2:490650/150‑1 (MQ=255)
      tCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcg                                                                                                                                              <  2:1275026/150‑1 (MQ=255)
       cACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcgg                                                                                                                                             <  2:1267032/150‑1 (MQ=255)
        aCCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggt                                                                                                                                            >  2:793415/1‑150 (MQ=255)
         ccagccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggtt                                                                                                                                           >  1:820166/1‑150 (MQ=255)
          cagccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttc                                                                                                                                          <  2:723859/150‑1 (MQ=255)
          cagccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttc                                                                                                                                          >  2:263065/1‑150 (MQ=255)
          cagccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttc                                                                                                                                          <  2:59185/150‑1 (MQ=255)
             ccagTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCtcggttcggt                                                                                                                                       <  2:466794/150‑1 (MQ=255)
               agTGGTGGCTGCCGTATGACCCACAGGCGATTGATATGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGATGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTaa                                                                                                                                     >  1:915011/1‑150 (MQ=255)
                 tggtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATg                                                                                                                                   <  2:1626497/150‑1 (MQ=255)
                 tggtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATg                                                                                                                                   <  1:190563/150‑1 (MQ=255)
                  ggtggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATgg                                                                                                                                  <  1:1389399/150‑1 (MQ=255)
                    tggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgcc                                                                                                                                <  2:1104904/150‑1 (MQ=255)
                    tggCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgcc                                                                                                                                <  1:1076873/150‑1 (MQ=255)
                      gCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgcctg                                                                                                                              <  1:1608690/150‑1 (MQ=255)
                       cTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTCTCGCTCGGTTCGGTAATGgcctgc                                                                                                                             >  1:989630/1‑150 (MQ=255)
                       cTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCGGATGGCAGCGCCCCGCGTGTCGCTCGGGTCGGTAATGgcctgc                                                                                                                             >  2:840407/1‑150 (MQ=255)
                        tGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCGGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGgcctgcc                                                                                                                            <  2:1359123/150‑1 (MQ=255)
                          ccGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGGGTCGCTCGGTTCGGTAATGGcctgcctg                                                                                                                          >  1:462679/1‑150 (MQ=255)
                                 aCCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTCGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTgg                                                                                                                   <  1:1242535/150‑1 (MQ=255)
                                  cccACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGt                                                                                                                  >  2:1440119/1‑150 (MQ=255)
                                    cacaGTAGATTGATTTGCGGTCGCGCCTGCTTTCGGCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTAGGTAATGGCCTGCCTGCTGCTGGTGc                                                                                                                <  2:1304904/150‑1 (MQ=255)
                                    cacaGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGc                                                                                                                >  1:829952/1‑150 (MQ=255)
                                     acaGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCCGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGGTCGGGTCGGTAATGGCCTGCCTGGAGCTGGGGcg                                                                                                               >  1:467698/1‑149 (MQ=255)
                                      caGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTg                                                                                                              <  1:1431862/150‑1 (MQ=255)
                                        ggCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGAc                                                                                                            >  2:303639/1‑150 (MQ=255)
                                          cGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACAt                                                                                                          >  2:1124756/1‑150 (MQ=255)
                                           gattgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACAtt                                                                                                         <  2:741510/150‑1 (MQ=255)
                                           gattgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCGCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACAtt                                                                                                         >  1:1507444/1‑150 (MQ=255)
                                             ttgattTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTAGGTTATGGCCTGCCTGCTGCTGGTGCTGACATGAg                                                                                                       >  1:1414023/1‑150 (MQ=255)
                                                attTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAgggg                                                                                                    >  1:779080/1‑149 (MQ=255)
                                                   tGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTgg                                                                                                 <  1:459271/150‑1 (MQ=255)
                                                   ggCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGGCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTgg                                                                                                 <  1:680247/149‑1 (MQ=255)
                                                    gCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGt                                                                                                <  1:1035473/150‑1 (MQ=255)
                                                       gTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGAGTAGCTCGGTTCGGTAATGGCCAGCCTGCTGCAGGTGCTGACATTAGGGCTGGatat                                                                                             >  2:562844/1‑150 (MQ=255)
                                                         cgcgCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTg                                                                                           <  2:474867/150‑1 (MQ=255)
                                                            gcCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACAGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCGGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcg                                                                                        <  2:1499581/150‑1 (MQ=255)
                                                            gcCGGCTTGCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATTTTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTGCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcg                                                                                        <  2:1499585/150‑1 (MQ=255)
                                                             ccTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTggcgg                                                                                       >  2:1481781/1‑150 (MQ=255)
                                                             ccTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGAGCACTTAGGTCGCGATATTTTCGCGCGGCGGATGGCAGCGGCCCGCGTGTCGCGCGGTTCGGGAATGGCCTGCCTGCTGCGGGGGCTGCCAGGAGGGCGGGGGAGTggcgg                                                                                       >  1:1089276/1‑150 (MQ=255)
                                                               tGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCa                                                                                     <  2:1378621/150‑1 (MQ=255)
                                                                 cTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAgc                                                                                   <  2:1507444/150‑1 (MQ=255)
                                                                     cGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCg                                                                               >  2:933953/1‑150 (MQ=255)
                                                                      gCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTGGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCgg                                                                              >  1:1376847/1‑150 (MQ=255)
                                                                       ccGGATGCGCAGCACTGGCTGGGCTCCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCggg                                                                             <  1:562844/150‑1 (MQ=255)
                                                                       ccGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCggg                                                                             <  2:915011/150‑1 (MQ=255)
                                                                         ggATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGtt                                                                           >  2:50036/1‑150 (MQ=255)
                                                                            ggcgcAGCACTGGCTGGGCACCGATCACTTAGGGCGCGATATTTTCTCGCGGCTGCTGGCAGCGACCCGCGTGGCGCTCGGTGCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGAt                                                                        <  2:855532/149‑1 (MQ=255)
                                                                             gcgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGAtt                                                                       >  1:561177/1‑150 (MQ=255)
                                                                              cgcAGCACTGGCTGGGCACCGTTCACTTAGGACGCGATATTTTCTCGCGGCTGATGGAAGCGACCCGCGAGACGCACGGTTCGGTTATGGCCTGCCGGCTGCTGGTGCTGACATTAGGGCTGGTTAATGGCGGCAGCGCCGGGTTGATTg                                                                      >  2:244457/1‑150 (MQ=255)
                                                                              cgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATCTTTTCTCGCGGCGGATGGCAGCGACCCGCGTGGCGCTCGATTCGGTAATGGCCGGCCTGCTGCTGGTGCTGACAGTAGGGCGGGTGATGGGCGGCAGCGCCGGGGTGCTTg                                                                      >  1:1043737/1‑150 (MQ=255)
                                                                              cgcAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCCGCGACCCGCGGGTCGCTCGATTCGGTAATGGCCTGCCAGCGGCTTGTGCTGACCTTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGAtgg                                                                      >  1:1038249/1‑148 (MQ=255)
                                                                                cagcaCTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggc                                                                    <  2:1436463/150‑1 (MQ=255)
                                                                                cagcaCTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGAGGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTggc                                                                    <  2:1063204/150‑1 (MQ=255)
                                                                                        gctggGCACCGAGCACTTCGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGt                                                            <  2:659266/150‑1 (MQ=255)
                                                                                         ctggGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGtt                                                           >  2:1553565/1‑150 (MQ=255)
                                                                                             gCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTTGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATc                                                       >  2:943514/1‑150 (MQ=255)
                                                                                                ccGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAgg                                                    <  1:1124756/150‑1 (MQ=255)
                                                                                                     cACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGccacc                                               <  1:1306401/150‑1 (MQ=255)
                                                                                                     cACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGccacc                                               >  2:896340/1‑150 (MQ=255)
                                                                                                       cTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGGTGGTTTTTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCAt                                             >  1:112332/1‑150 (MQ=255)
                                                                                                         tAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATgc                                           <  1:793415/150‑1 (MQ=255)
                                                                                                             tCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGt                                       <  1:1553565/150‑1 (MQ=255)
                                                                                                                cgATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGc                                    >  2:175979/1‑150 (MQ=255)
                                                                                                                  atatTTTCTCGCGGCTGATGGCAGCGACCCGCGTGGCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCg                                  <  2:1216599/150‑1 (MQ=255)
                                                                                                                    atTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGat                                >  2:779572/1‑150 (MQ=255)
                                                                                                                     ttttCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCGGGGTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGata                               <  2:1215220/150‑1 (MQ=255)
                                                                                                                        tctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATTCGCGTCGCAGATATGt                            >  2:852665/1‑150 (MQ=255)
                                                                                                                        tctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGt                            >  2:846978/1‑150 (MQ=255)
                                                                                                                         ctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGtt                           <  1:987377/150‑1 (MQ=255)
                                                                                                                         ctcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGACTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGtt                           <  2:829952/150‑1 (MQ=255)
                                                                                                                          tcGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGttt                          >  1:1179224/1‑150 (MQ=255)
                                                                                                                           cgcgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTa                         >  2:978480/1‑150 (MQ=255)
                                                                                                                           cgcgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTa                         <  2:561177/150‑1 (MQ=255)
                                                                                                                           cgcgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCGGGTTATTGGCTGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATAGGTTTa                         >  1:683516/1‑150 (MQ=255)
                                                                                                                             cgGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGCTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATg                       >  2:230024/1‑150 (MQ=255)
                                                                                                                             cgGCTGATGGCAGCGACCCGCGTGTCGCTCGGGTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATg                       <  1:1339621/150‑1 (MQ=255)
                                                                                                                              ggCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGa                      >  2:1644185/1‑150 (MQ=255)
                                                                                                                                cTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCGGACCTTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGGCGCCGATATGTTTATGAcc                    <  1:303639/150‑1 (MQ=255)
                                                                                                                                  gaaccgaGCGACACGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCtt                  >  2:1469351/7‑150 (MQ=255)
                                                                                                                                  gATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCtt                  >  1:500932/1‑150 (MQ=255)
                                                                                                                                  gATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGGTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCtt                  >  1:1135658/1‑150 (MQ=255)
                                                                                                                                    tGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCGTGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTcc                <  2:845936/150‑1 (MQ=255)
                                                                                                                                     ccgaGCGACACGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTccc               >  1:1184988/4‑150 (MQ=255)
                                                                                                                                      gCAGCGAACCGGGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTTGTGCTGACATTAGGGCTGGTTCTTGGCGGCAGCGCCGGGTGGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCg              <  2:683516/150‑1 (MQ=255)
                                                                                                                                       cAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGa             <  2:149261/150‑1 (MQ=255)
                                                                                                                                        aGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGAc            <  1:1440119/150‑1 (MQ=255)
                                                                                                                                         gctaCCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGGTGGGGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTTGCGGGCGCGTTTATCAGGACACCTTGAGCGTCGACGATATGTTTATGTCCTTCCCGAcc           <  2:1541024/147‑1 (MQ=255)
                                                                                                                                         gCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGAcc           >  1:18159/1‑150 (MQ=255)
                                                                                                                                         gCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGAcc           <  2:24665/150‑1 (MQ=255)
                                                                                                                                         gCGACCCGCGTGTCGCGCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGAcc           <  2:1093884/150‑1 (MQ=255)
                                                                                                                                           gACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTc         <  1:933953/150‑1 (MQ=255)
                                                                                                                                           gACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTc         >  2:1018718/1‑150 (MQ=255)
                                                                                                                                           gACCCGCGGGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTc         <  2:230989/150‑1 (MQ=255)
                                                                                                                                                  gtgtCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGCCCTTCCCGCCCTCGATTCTg  >  1:1109646/1‑150 (MQ=255)
                                                                                                                                                  gtgtCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTg  <  1:1430794/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGCGCTCACCAGCCAGTGGTGGCTGCCGTATGACCCACAGGCGATTGATTTGCCGTCGCGCCTGCTTTCGCCGGATGCGCAGCACTGGCTGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCCTGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTG  >  NC_000913/3616252‑3616547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑