Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,632,764 G→A 6.2% intergenic (‑174/+88) yhiJ ← / ← yhiL DUF4049 family protein/pseudogene, DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,632,7640GA 6.2% 403.7 / 8.9 129intergenic (‑174/+88)yhiJ/yhiLDUF4049 family protein/pseudogene, DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base G (62/58);  new base A (4/4);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01

Only 100 of 132 total aligned reads displayed.

GAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGT  >  NC_000913/3632615‑3632912
                                                                                                                                                     |                                                                                                                                                    
taGGAATAGATAGTTAACATTTTATCTTATTACTTACACCTCTTGTTCGATGGGATAATAATTATATTTTGCTCAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAATATTGTAGAGGCg                                                                                                                                                      <  1:1372533/149‑1 (MQ=255)
gAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCg                                                                                                                                                      <  2:898988/150‑1 (MQ=255)
gAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCg                                                                                                                                                      >  2:461371/1‑150 (MQ=255)
   gAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGtca                                                                                                                                                   <  1:1616203/150‑1 (MQ=255)
        cATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGACCCTAAAGATTGTAGAGGCGTCATCAGa                                                                                                                                              >  2:234874/1‑150 (MQ=255)
            gTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACAt                                                                                                                                          >  2:400555/1‑150 (MQ=255)
              tAACATTTTATCTTATGACATTCACCTCATGTTCGATGGGAAAATAATTATATTTTTCACAAATAATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCATAGAAAGTCACTAAAGATTTTATAGGCGTAATCAGAAAAtta                                                                                                                                        >  1:35268/1‑148 (MQ=255)
              tAACATTTTATCTTATGACATACCCCTCATGTGCGCTGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                        >  1:335024/1‑150 (MQ=255)
              tAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                        >  2:686213/1‑150 (MQ=255)
               aaCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGcc                                                                                                                                       <  1:973324/150‑1 (MQ=255)
               aaCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGcc                                                                                                                                       >  1:1373228/1‑150 (MQ=255)
                 cATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCctc                                                                                                                                     <  2:1433689/150‑1 (MQ=255)
                    tttATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTAc                                                                                                                                  <  1:580458/150‑1 (MQ=255)
                    tttATCTTATGACATACACCTCATGTTAGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGTCGTCATCAGAACATGCCTCTAc                                                                                                                                  <  1:1565425/150‑1 (MQ=255)
                     ttATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCAACa                                                                                                                                 >  2:1445739/1‑150 (MQ=255)
                      tATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTACaa                                                                                                                                <  2:1576861/150‑1 (MQ=255)
                         cTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTACAATct                                                                                                                             <  2:793120/150‑2 (MQ=38)
                         attATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCg                                                                                                                             <  2:1661056/149‑1 (MQ=255)
                           tATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAt                                                                                                                           >  2:1234425/1‑150 (MQ=255)
                           tATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAt                                                                                                                           >  1:966446/1‑150 (MQ=255)
                                    cacCTCATGTTCGATGTGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAATCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAc                                                                                                                  <  2:1663369/150‑1 (MQ=255)
                                    cacCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAc                                                                                                                  >  1:968549/1‑150 (MQ=255)
                                      ccTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCt                                                                                                                >  2:1221505/1‑150 (MQ=255)
                                           tGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGATAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGaa                                                                                                           <  1:1282598/150‑1 (MQ=255)
                                           tGTTCGATGGGAAAATAATTATATTTTGAACAAATCATTCAGCCTCAAAGCATAATATGAAATTTATTATCTAATAGCAGAGAAAGTCCCTAAAGATTGTATAGGCGTCATCAGAACATGCCTCTACAATAGATTAAGAAAACCTATGaa                                                                                                           >  1:331801/1‑150 (MQ=255)
                                               cGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATAc                                                                                                       >  1:1292406/1‑150 (MQ=255)
                                                  tGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTgg                                                                                                    >  1:1319593/1‑150 (MQ=255)
                                                   gggAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGACATACTGGc                                                                                                   <  1:1076056/150‑1 (MQ=255)
                                                    ggAAAATAATATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                 >  1:372160/1‑150 (MQ=255)
                                                     gAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                 >  1:1027405/1‑150 (MQ=255)
                                                     gAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                 <  1:1420109/150‑1 (MQ=255)
                                                        aataatTATATTTTTCACAAATCATTCAGGCTAAAAGCATAATATGAAATTTCTTATCTCATATTAGTGAAAGTTCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGAt                                                                                              <  2:3228/150‑1 (MQ=255)
                                                        aataatTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATATCAGAGAAAGTCCCTAAAGATTTTAGAGGCGTCATCAGAACATGCCTCTACAATAGATTAAGATAACCAATGAAATACTGTCAGGAt                                                                                              >  1:299587/1‑150 (MQ=255)
                                                           aatTATATTTTGCACAAATCATTCACCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaa                                                                                           <  1:272578/150‑1 (MQ=255)
                                                            atTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaa                                                                                          <  2:384457/150‑1 (MQ=255)
                                                            atTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaa                                                                                          <  2:646660/150‑1 (MQ=255)
                                                             ttATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaa                                                                                         >  2:1352100/1‑150 (MQ=255)
                                                              tataTTTTGCACAAATCATGCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaaa                                                                                        <  1:471636/150‑1 (MQ=255)
                                                                tatTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGa                                                                                      <  1:1653844/150‑1 (MQ=255)
                                                                tatTTTGCACAAATCATTCAGCATCAAAGCATAATATGATATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCTTCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGa                                                                                      <  2:331801/150‑1 (MQ=255)
                                                                 atTTTGCACAAATCATTCAGCCTCAAAGCAGAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGAc                                                                                     >  1:684937/1‑150 (MQ=255)
                                                                   tttGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACAt                                                                                   <  2:1086195/150‑1 (MQ=255)
                                                                      gCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATacc                                                                                <  1:1221505/150‑1 (MQ=255)
                                                                      gCACAAATCATTCAGCCTCAAAGCATAATATGAAAATTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAACAATGAAATACTGGCAGGAATAAAAAGACATaca                                                                                >  1:1693561/1‑149 (MQ=255)
                                                                       aacaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATacca                                                                               <  1:1493875/149‑1 (MQ=255)
                                                                        acaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATaccac                                                                              >  1:1292376/1‑150 (MQ=255)
                                                                          aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTa                                                                                            <  1:739453/134‑1 (MQ=255)
                                                                          aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCg                                                                            >  1:827352/1‑150 (MQ=255)
                                                                          aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCg                                                                            <  2:1373228/150‑1 (MQ=255)
                                                                                   aGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATgg                                                                   <  2:394406/150‑1 (MQ=255)
                                                                                    gCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGa                                                                  >  1:1349486/1‑150 (MQ=255)
                                                                                       tCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCAATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGta                                                               >  2:100097/1‑149 (MQ=255)
                                                                                            gCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTGTACAATCGATTAAGAAAACCTATGAAATCCGGGCAGGATTAAAACGACATACCACCGTATTGAGGGAGGTTTAAt                                                          >  2:829551/1‑150 (MQ=255)
                                                                                              ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                        <  2:684937/150‑1 (MQ=255)
                                                                                              ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                        <  2:335024/150‑1 (MQ=255)
                                                                                              ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                        <  2:1292406/150‑1 (MQ=255)
                                                                                                aataTGAAATTTCTTATCTCATAGCAGAGAAAGTCACTAAAGATTGTAGAGGCTTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAg                                                      <  1:1529509/150‑1 (MQ=255)
                                                                                                 atatGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGt                                                     >  1:1099788/1‑150 (MQ=255)
                                                                                                  tatGAAATTTCTTATCTCATTGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAAAATGCCTCTACAATCGAATAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGtt                                                    >  2:539688/1‑150 (MQ=255)
                                                                                                   atGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTg                                                   >  2:103627/1‑150 (MQ=255)
                                                                                                     taaaTTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGaa                                                 <  2:1272227/149‑1 (MQ=255)
                                                                                                         tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                             <  2:827352/150‑1 (MQ=255)
                                                                                                         tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                             >  1:693348/1‑150 (MQ=255)
                                                                                                         tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                             <  1:573937/150‑1 (MQ=255)
                                                                                                         tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                             <  2:693348/150‑1 (MQ=255)
                                                                                                           tCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaacaac                                           <  2:123385/150‑1 (MQ=255)
                                                                                                               atatcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                       <  2:1077201/147‑1 (MQ=255)
                                                                                                               aTCTCATAGAAGAGAAAGTACCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTTGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                       <  2:1083067/150‑1 (MQ=255)
                                                                                                                tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                      <  1:1345341/150‑1 (MQ=255)
                                                                                                                tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                      <  2:968549/150‑1 (MQ=255)
                                                                                                                tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGAAGTTGAAAACAACTAAGc                                      <  2:966446/150‑1 (MQ=255)
                                                                                                                 ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                     >  1:247861/1‑150 (MQ=255)
                                                                                                                 ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                     >  2:1054736/1‑150 (MQ=255)
                                                                                                                 ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                     >  1:1529340/1‑150 (MQ=255)
                                                                                                                  tcATTGCAGAGAAAGTCCATAAAGATTGTAGAGGCGTCAGCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGTCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                    <  1:1130708/150‑1 (MQ=255)
                                                                                                                  tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                    >  1:1375010/1‑150 (MQ=255)
                                                                                                                  tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                    <  1:1000672/150‑1 (MQ=255)
                                                                                                                  tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                    >  2:54896/1‑150 (MQ=255)
                                                                                                                   cATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCt                                   >  2:663930/1‑150 (MQ=255)
                                                                                                                    aTAGCAGAGAAAGTCCCTACAGATTGTAGAGCCGTCATCAGAACATGCCGCTACAAGAGATGAAGAAAACCTAAGAAATACTGGCAGGAATATAAGGACATACCACCGTATTCATGGAGTTTTAAGGTAGTTGAATACAACTAATCCCTg                                  >  1:74036/1‑150 (MQ=255)
                                                                                                                     tAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGa                                 >  1:1544593/1‑150 (MQ=255)
                                                                                                                      aGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGACCTGAg                                >  2:1307763/1‑150 (MQ=255)
                                                                                                                       gCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAgg                               <  1:54896/150‑1 (MQ=255)
                                                                                                                        cAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGa                              >  1:996126/1‑150 (MQ=255)
                                                                                                                         agagAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGaa                             >  1:1448105/1‑150 (MQ=255)
                                                                                                                               aGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAatt                       <  2:1349486/150‑1 (MQ=255)
                                                                                                                               aGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAatt                       >  1:174873/1‑150 (MQ=255)
                                                                                                                                   ccTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattt                   >  1:504229/1‑150 (MQ=255)
                                                                                                                                    taatcgATTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttt                  >  2:224318/6‑150 (MQ=38)
                                                                                                                                    taatcgATTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttt                  >  2:202653/6‑150 (MQ=38)
                                                                                                                                     tAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttta                 >  1:846153/1‑150 (MQ=255)
                                                                                                                                      aaaGATTGTCGAGGCGGCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAACTACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttat                <  2:228901/150‑1 (MQ=255)
                                                                                                                                        aGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttatta              >  1:819590/1‑150 (MQ=255)
                                                                                                                                        aGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttatta              >  1:1049302/1‑150 (MQ=255)
                                                                                                                                          aTTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatt            >  2:630240/1‑150 (MQ=39)
                                                                                                                                           ttGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattattt           <  1:224318/150‑1 (MQ=255)
                                                                                                                                                gagGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATc      >  1:1610273/1‑150 (MQ=255)
                                                                                                                                                  ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatttt          >  2:15476/1‑144 (MQ=255)
                                                                                                                                                  ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCAc    <  1:461371/150‑1 (MQ=255)
                                                                                                                                                    cGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCAATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGt  >  1:460862/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAGGAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGT  >  NC_000913/3632615‑3632912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: