Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,632,766 C→G 6.4% intergenic (‑176/+86) yhiJ ← / ← yhiL DUF4049 family protein/pseudogene, DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,632,7660CG 6.4% 414.8 / 14.4 125intergenic (‑176/+86)yhiJ/yhiLDUF4049 family protein/pseudogene, DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base C (60/56);  new base G (4/4);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 131 total aligned reads displayed.

GAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGCT  >  NC_000913/3632618‑3632915
                                                                                                                                                    |                                                                                                                                                     
gAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGtca                                                                                                                                                      <  1:1616203/150‑1 (MQ=255)
 aaTACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAATATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGtcat                                                                                                                                                     >  1:910447/1‑150 (MQ=255)
 aaTACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGtcat                                                                                                                                                     <  1:1399244/150‑1 (MQ=255)
     cATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGACCCTAAAGATTGTAGAGGCGTCATCAGa                                                                                                                                                 >  2:234874/1‑150 (MQ=255)
         gTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACAt                                                                                                                                             >  2:400555/1‑150 (MQ=255)
          ttAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATg                                                                                                                                            <  1:1639061/150‑1 (MQ=255)
           tAACATTTTATCTTATGACATTCACCTCATGTTCGATGGGAAAATAATTATATTTTTCACAAATAATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCATAGAAAGTCACTAAAGATTTTATAGGCGTAATCAGAAAAtta                                                                                                                                           >  1:35268/1‑148 (MQ=255)
           tAACATTTTATCTTATGACATACCCCTCATGTGCGCTGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                           >  1:335024/1‑150 (MQ=255)
           tAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                           >  2:192996/1‑150 (MQ=255)
           tAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                           >  2:686213/1‑150 (MQ=255)
            aaCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGcc                                                                                                                                          <  1:973324/150‑1 (MQ=255)
            aaCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGcc                                                                                                                                          >  1:1373228/1‑150 (MQ=255)
             aCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCct                                                                                                                                         >  2:1420109/1‑150 (MQ=255)
                ttatagcTTATGACATACACCCCATGATCGATGGGAAAATAATTATATTTTGCCCAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCATAGAAAGTCCCTAAATATTGTAGATTCGTCAGCATCACATGCCTCTa                                                                                                                                      >  2:1130708/7‑150 (MQ=255)
                 tttATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTAc                                                                                                                                     <  1:580458/150‑1 (MQ=255)
                  ttATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCAACa                                                                                                                                    >  2:1445739/1‑150 (MQ=255)
                   tATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTACaa                                                                                                                                   <  2:1576861/150‑1 (MQ=255)
                     tCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATc                                                                                                                                 <  2:551836/150‑1 (MQ=255)
                      cTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTACAATct                                                                                                                                <  2:793120/150‑2 (MQ=38)
                        tATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGAt                                                                                                                              >  2:1234425/1‑150 (MQ=255)
                               tACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGaaa                                                                                                                       >  2:1076056/1‑150 (MQ=255)
                                 cacCTCATGTTCGATGTGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAATCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAc                                                                                                                     <  2:1663369/150‑1 (MQ=255)
                                 cacCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAc                                                                                                                     >  1:968549/1‑150 (MQ=255)
                                      cATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATg                                                                                                                <  2:322304/150‑1 (MQ=255)
                                        tGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGATAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGaa                                                                                                              <  1:1282598/150‑1 (MQ=255)
                                        tGTTCGATGGGAAAATAATTATATTTTGAACAAATCATTCAGCCTCAAAGCATAATATGAAATTTATTATCTAATAGCAGAGAAAGTCCCTAAAGATTGTATAGGCGTCATCAGAACATGCCTCTACAATAGATTAAGAAAACCTATGaa                                                                                                              >  1:331801/1‑150 (MQ=255)
                                               tGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTgg                                                                                                       >  1:1319593/1‑150 (MQ=255)
                                                gggAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGACATACTGGc                                                                                                      <  1:1076056/150‑1 (MQ=255)
                                                 ggAAAATAATATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                    >  1:372160/1‑150 (MQ=255)
                                                  gAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                    >  1:1027405/1‑150 (MQ=255)
                                                     aataatTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATATCAGAGAAAGTCCCTAAAGATTTTAGAGGCGTCATCAGAACATGCCTCTACAATAGATTAAGATAACCAATGAAATACTGTCAGGAt                                                                                                 >  1:299587/1‑150 (MQ=255)
                                                        aatTATATTTTGCACAAATCATTCACCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaa                                                                                              <  1:272578/150‑1 (MQ=255)
                                                         atTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaa                                                                                             <  2:646660/150‑1 (MQ=255)
                                                          ttATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaa                                                                                            >  2:1345341/1‑150 (MQ=255)
                                                          ttATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaa                                                                                            >  2:1352100/1‑150 (MQ=255)
                                                           tataTTTTGCACAAATCATGCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaaa                                                                                           <  1:471636/150‑1 (MQ=255)
                                                             tatTTTGCACAAATCATTCAGCATCAAAGCATAATATGATATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCTTCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGa                                                                                         <  2:331801/150‑1 (MQ=255)
                                                              atTTTGCACAAATCATTCAGCCTCAAAGCAGAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGAc                                                                                        >  1:684937/1‑150 (MQ=255)
                                                                tttGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACAt                                                                                      <  2:1086195/150‑1 (MQ=255)
                                                                 ttGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATa                                                                                     <  1:1097306/150‑1 (MQ=255)
                                                                   gCACAAATCATTCAGCCTCAAAGCATAATATGAAAATTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAACAATGAAATACTGGCAGGAATAAAAAGACATaca                                                                                   >  1:1693561/1‑149 (MQ=255)
                                                                    cacaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCAAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGAAGGATTAAAAAGACATacca                                                                                  >  1:794360/1‑150 (MQ=255)
                                                                    aacaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATacca                                                                                  <  1:1493875/149‑1 (MQ=255)
                                                                     acaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATaccac                                                                                 >  1:1292376/1‑150 (MQ=255)
                                                                       aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTa                                                                                               <  1:739453/134‑1 (MQ=255)
                                                                       aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTa                                                                                               >  2:739453/1‑134 (MQ=255)
                                                                       aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCg                                                                               >  1:827352/1‑150 (MQ=255)
                                                                       aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCg                                                                               <  2:1373228/150‑1 (MQ=255)
                                                                                aGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATgg                                                                      <  2:394406/150‑1 (MQ=255)
                                                                                 gCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGTTTGTAGAGGCGTCATCAGAACATGCCTCTACAAACGTTTAAGAAAACCTATGAAATACTGGAATGATTAAAAAGACATACCACAGTATTCATggt                                                                     >  2:1271734/1‑149 (MQ=255)
                                                                                    tCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGtt                                                                  >  1:1238330/1‑150 (MQ=255)
                                                                                    tCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCAATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGta                                                                  >  2:100097/1‑149 (MQ=255)
                                                                                           ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                           <  2:1292406/150‑1 (MQ=255)
                                                                                           ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                           <  2:684937/150‑1 (MQ=255)
                                                                                           ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                           <  2:335024/150‑1 (MQ=255)
                                                                                            taataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTa                                                          >  2:157065/1‑150 (MQ=255)
                                                                                             aataTGAAATTTCTTATCTCATAGCAGAGAAAGTCACTAAAGATTGTAGAGGCTTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAg                                                         <  1:1529509/150‑1 (MQ=255)
                                                                                              atatGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGt                                                        >  1:1099788/1‑150 (MQ=255)
                                                                                               tatGAAATTTCTTATCTCATTGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAAAATGCCTCTACAATCGAATAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGtt                                                       >  2:539688/1‑150 (MQ=255)
                                                                                                atGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTg                                                      >  2:103627/1‑150 (MQ=255)
                                                                                                      tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                                <  1:573937/150‑1 (MQ=255)
                                                                                                      tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                                <  2:827352/150‑1 (MQ=255)
                                                                                                        tCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaacaac                                              <  2:123385/150‑1 (MQ=255)
                                                                                                            aTCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                          <  2:664759/150‑1 (MQ=255)
                                                                                                            aTCTCATAGAAGAGAAAGTACCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTTGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                          <  2:1083067/150‑1 (MQ=255)
                                                                                                             tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                         <  2:968549/150‑1 (MQ=255)
                                                                                                             tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                         <  1:1302131/150‑1 (MQ=255)
                                                                                                             tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                         <  1:1345341/150‑1 (MQ=255)
                                                                                                             tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                         <  1:1352100/150‑1 (MQ=255)
                                                                                                             tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGAAGTTGAAAACAACTAAGc                                         <  2:966446/150‑1 (MQ=255)
                                                                                                              ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                        >  2:1054736/1‑150 (MQ=255)
                                                                                                              ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                        >  1:247861/1‑150 (MQ=255)
                                                                                                               tcATTGCAGAGAAAGTCCATAAAGATTGTAGAGGCGTCAGCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGTCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                       <  1:1130708/150‑1 (MQ=255)
                                                                                                               tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                       <  1:1000672/150‑1 (MQ=255)
                                                                                                               tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                       >  1:1375010/1‑150 (MQ=255)
                                                                                                                cATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCt                                      <  2:407927/150‑1 (MQ=255)
                                                                                                                 aTAGCAGAGAAAGTCCCTACAGATTGTAGAGCCGTCATCAGAACATGCCGCTACAAGAGATGAAGAAAACCTAAGAAATACTGGCAGGAATATAAGGACATACCACCGTATTCATGGAGTTTTAAGGTAGTTGAATACAACTAATCCCTg                                     >  1:74036/1‑150 (MQ=255)
                                                                                                                  tAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGa                                    >  1:1544593/1‑150 (MQ=255)
                                                                                                                   aGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGACCTGAg                                   >  2:1307763/1‑150 (MQ=255)
                                                                                                                    gCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAgg                                  <  1:54896/150‑1 (MQ=255)
                                                                                                                     cAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGa                                 >  1:996126/1‑150 (MQ=255)
                                                                                                                     cAGAGAAAGTCCATAAAGATTGTTGATGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGa                                 <  2:35268/150‑1 (MQ=255)
                                                                                                                      agagAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGaa                                >  1:1448105/1‑150 (MQ=255)
                                                                                                                      agagAAAGTCCCTAAAGATTGTAGAGGAGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGaa                                <  1:229957/150‑1 (MQ=255)
                                                                                                                       gagaAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAAt                               >  2:832955/1‑150 (MQ=255)
                                                                                                                            aGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAatt                          >  1:174873/1‑150 (MQ=255)
                                                                                                                                ccTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattt                      >  1:504229/1‑150 (MQ=255)
                                                                                                                                 taatcgATTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttt                     >  2:202653/6‑150 (MQ=38)
                                                                                                                                 cTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGTATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttt                     <  1:686213/150‑1 (MQ=255)
                                                                                                                                  tAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttta                    >  1:846153/1‑150 (MQ=255)
                                                                                                                                   aaaGATTGTCGAGGCGGCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAACTACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttat                   <  2:228901/150‑1 (MQ=255)
                                                                                                                                     aGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttatta                 >  1:1049302/1‑150 (MQ=255)
                                                                                                                                       aTTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatt               >  2:630240/1‑150 (MQ=39)
                                                                                                                                        ttGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattattt              <  1:224318/150‑1 (MQ=255)
                                                                                                                                             gagGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATc         >  1:1494365/1‑150 (MQ=255)
                                                                                                                                             gagGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATc         >  1:1610273/1‑150 (MQ=255)
                                                                                                                                               ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatttt             >  2:15476/1‑144 (MQ=255)
                                                                                                                                               ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatttt             <  1:15476/144‑1 (MQ=255)
                                                                                                                                               ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCAc       <  1:461371/150‑1 (MQ=255)
                                                                                                                                                 cGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCAATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGt     >  1:460862/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAATACATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGCT  >  NC_000913/3632618‑3632915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: