Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,632,770 A→T 6.4% intergenic (‑180/+82) yhiJ ← / ← yhiL DUF4049 family protein/pseudogene, DUF4049 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,632,7700AT 6.4% 365.7 / 10.2 125intergenic (‑180/+82)yhiJ/yhiLDUF4049 family protein/pseudogene, DUF4049 family protein
Reads supporting (aligned to +/- strand):  ref base A (61/56);  new base T (4/4);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

Only 100 of 134 total aligned reads displayed.

CATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGCTTCCT  >  NC_000913/3632623‑3632919
                                                                                                                                                   |                                                                                                                                                     
cATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGACCCTAAAGATTGTAGAGGCGTCATCAGa                                                                                                                                                     >  2:234874/1‑150 (MQ=255)
    gTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACAt                                                                                                                                                 >  2:400555/1‑150 (MQ=255)
      tAACATTTTATCTTATGACATTCACCTCATGTTCGATGGGAAAATAATTATATTTTTCACAAATAATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCATAGAAAGTCACTAAAGATTTTATAGGCGTAATCAGAAAAtta                                                                                                                                               >  1:35268/1‑148 (MQ=255)
      tAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                               >  2:686213/1‑150 (MQ=255)
      tAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGc                                                                                                                                               >  2:192996/1‑150 (MQ=255)
       aaCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGcc                                                                                                                                              <  1:973324/150‑1 (MQ=255)
        aCATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCct                                                                                                                                             >  2:1420109/1‑150 (MQ=255)
         cATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCctc                                                                                                                                            <  2:1433689/150‑1 (MQ=255)
           ttatagcTTATGACATACACCCCATGATCGATGGGAAAATAATTATATTTTGCCCAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCATAGAAAGTCCCTAAATATTGTAGATTCGTCAGCATCACATGCCTCTa                                                                                                                                          >  2:1130708/7‑150 (MQ=255)
            tttATCTTATGACATACACCTCATGTTAGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGTCGTCATCAGAACATGCCTCTAc                                                                                                                                         <  1:1565425/150‑1 (MQ=255)
             ttATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCAACa                                                                                                                                        >  2:1445739/1‑150 (MQ=255)
              tATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCATGTTCTGATGACGCCTCTACaa                                                                                                                                       <  2:1576861/150‑1 (MQ=255)
                tCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATc                                                                                                                                     <  2:551836/150‑1 (MQ=255)
                          tACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGaaa                                                                                                                           >  2:1076056/1‑150 (MQ=255)
                            cacCTCATGTTCGATGTGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAATCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAc                                                                                                                         <  2:1663369/150‑1 (MQ=255)
                            cacCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAc                                                                                                                         >  1:968549/1‑150 (MQ=255)
                              ccTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCt                                                                                                                       >  2:1221505/1‑150 (MQ=255)
                                   tGTTCGATGGGAAAATAATTATATTTTGAACAAATCATTCAGCCTCAAAGCATAATATGAAATTTATTATCTAATAGCAGAGAAAGTCCCTAAAGATTGTATAGGCGTCATCAGAACATGCCTCTACAATAGATTAAGAAAACCTATGaa                                                                                                                  >  1:331801/1‑150 (MQ=255)
                                       cGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATAc                                                                                                              >  1:1292406/1‑150 (MQ=255)
                                           gggAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGACATACTGGc                                                                                                          <  1:1076056/150‑1 (MQ=255)
                                            ggAAAATAATATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                        >  1:372160/1‑150 (MQ=255)
                                             gAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                        >  1:1027405/1‑150 (MQ=255)
                                             gAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAg                                                                                                        <  1:1420109/150‑1 (MQ=255)
                                                aataatTATATTTTTCACAAATCATTCAGGCTAAAAGCATAATATGAAATTTCTTATCTCATATTAGTGAAAGTTCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGAt                                                                                                     <  2:3228/150‑1 (MQ=255)
                                                aataatTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATATCAGAGAAAGTCCCTAAAGATTTTAGAGGCGTCATCAGAACATGCCTCTACAATAGATTAAGATAACCAATGAAATACTGTCAGGAt                                                                                                     >  1:299587/1‑150 (MQ=255)
                                                    atTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaa                                                                                                 <  2:384457/150‑1 (MQ=255)
                                                    atTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaa                                                                                                 <  2:646660/150‑1 (MQ=255)
                                                     ttATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaa                                                                                                >  2:1345341/1‑150 (MQ=255)
                                                     ttATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaa                                                                                                >  2:1352100/1‑150 (MQ=255)
                                                      tataTTTTGCACAAATCATGCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTaaaaa                                                                                               <  1:471636/150‑1 (MQ=255)
                                                        tatTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGa                                                                                             <  1:1653844/150‑1 (MQ=255)
                                                        tatTTTGCACAAATCATTCAGCATCAAAGCATAATATGATATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCTTCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGa                                                                                             <  2:331801/150‑1 (MQ=255)
                                                         atTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGAc                                                                                            <  1:466808/150‑1 (MQ=255)
                                                         atTTTGCACAAATCATTCAGCCTCAAAGCAGAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGAc                                                                                            >  1:684937/1‑150 (MQ=255)
                                                            ttGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATa                                                                                         <  1:1097306/150‑1 (MQ=255)
                                                              gCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATacc                                                                                       <  1:1221505/150‑1 (MQ=255)
                                                              gCACAAATCATTCAGCCTCAAAGCATAATATGAAAATTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAAACAATGAAATACTGGCAGGAATAAAAAGACATaca                                                                                       >  1:1693561/1‑149 (MQ=255)
                                                               cacaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCAAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGAAGGATTAAAAAGACATacca                                                                                      >  1:794360/1‑150 (MQ=255)
                                                                acaAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATaccac                                                                                     >  1:1292376/1‑150 (MQ=255)
                                                                 caAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATaccacc                                                                                    >  2:1000672/1‑150 (MQ=255)
                                                                  aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTa                                                                                                   >  2:739453/1‑134 (MQ=255)
                                                                  aaaTCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCg                                                                                   >  1:827352/1‑150 (MQ=255)
                                                                           aGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATgg                                                                          <  2:394406/150‑1 (MQ=255)
                                                                            gCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGTTTGTAGAGGCGTCATCAGAACATGCCTCTACAAACGTTTAAGAAAACCTATGAAATACTGGAATGATTAAAAAGACATACCACAGTATTCATggt                                                                         >  2:1271734/1‑149 (MQ=255)
                                                                            gCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGa                                                                         >  1:1349486/1‑150 (MQ=255)
                                                                               tCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGtt                                                                      >  1:1238330/1‑150 (MQ=255)
                                                                               tCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCAATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGta                                                                      >  2:100097/1‑149 (MQ=255)
                                                                                    gCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTGTACAATCGATTAAGAAAACCTATGAAATCCGGGCAGGATTAAAACGACATACCACCGTATTGAGGGAGGTTTAAt                                                                 >  2:829551/1‑150 (MQ=255)
                                                                                      ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                               <  2:1292406/150‑1 (MQ=255)
                                                                                      ataataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGt                                                               <  2:335024/150‑1 (MQ=255)
                                                                                       taataTGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTa                                                              >  2:157065/1‑150 (MQ=255)
                                                                                        aataTGAAATTTCTTATCTCATAGCAGAGAAAGTCACTAAAGATTGTAGAGGCTTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAg                                                             <  1:1529509/150‑1 (MQ=255)
                                                                                          tatGAAATTTCTTATCTCATTGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAAAATGCCTCTACAATCGAATAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGtt                                                           >  2:539688/1‑150 (MQ=255)
                                                                                           atGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTg                                                          >  2:103627/1‑150 (MQ=255)
                                                                                             taaaTTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGaa                                                        <  2:1272227/149‑1 (MQ=255)
                                                                                                 tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                                    <  1:573937/150‑1 (MQ=255)
                                                                                                 tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                                    >  1:693348/1‑150 (MQ=255)
                                                                                                 tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                                    <  2:827352/150‑1 (MQ=255)
                                                                                                 tttCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaaca                                                    <  2:693348/150‑1 (MQ=255)
                                                                                                   tCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAaacaac                                                  <  2:123385/150‑1 (MQ=255)
                                                                                                       atatcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                              <  2:1077201/147‑1 (MQ=255)
                                                                                                       aTCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                              <  2:664759/150‑1 (MQ=255)
                                                                                                       aTCTCATAGAAGAGAAAGTACCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTTGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAg                                              <  2:1083067/150‑1 (MQ=255)
                                                                                                        tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                             <  1:1345341/150‑1 (MQ=255)
                                                                                                        tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                             <  1:1302131/150‑1 (MQ=255)
                                                                                                        tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                             <  2:968549/150‑1 (MQ=255)
                                                                                                        tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGc                                             <  1:1352100/150‑1 (MQ=255)
                                                                                                        tctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGAAGTTGAAAACAACTAAGc                                             <  2:966446/150‑1 (MQ=255)
                                                                                                         ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                            >  1:247861/1‑150 (MQ=255)
                                                                                                         ctcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGcc                                            >  1:1529340/1‑150 (MQ=255)
                                                                                                          tcATTGCAGAGAAAGTCCATAAAGATTGTAGAGGCGTCAGCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGTCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                           <  1:1130708/150‑1 (MQ=255)
                                                                                                          tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                           >  2:54896/1‑150 (MQ=255)
                                                                                                          tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                           >  1:1375010/1‑150 (MQ=255)
                                                                                                          tcATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGccc                                           <  1:1000672/150‑1 (MQ=255)
                                                                                                           cATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCt                                          >  2:663930/1‑150 (MQ=255)
                                                                                                           aaTAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCt                                          <  1:362450/149‑1 (MQ=255)
                                                                                                            aTAGCAGAGAAAGTCCCTACAGATTGTAGAGCCGTCATCAGAACATGCCGCTACAAGAGATGAAGAAAACCTAAGAAATACTGGCAGGAATATAAGGACATACCACCGTATTCATGGAGTTTTAAGGTAGTTGAATACAACTAATCCCTg                                         >  1:74036/1‑150 (MQ=255)
                                                                                                               gCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAgg                                      <  1:54896/150‑1 (MQ=255)
                                                                                                                 agagAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGaa                                    >  1:1448105/1‑150 (MQ=255)
                                                                                                                 agagAAAGTCCCTAAAGATTGTAGAGGAGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGaa                                    <  1:229957/150‑1 (MQ=255)
                                                                                                                  gagaAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAAt                                   >  2:832955/1‑150 (MQ=255)
                                                                                                                       aGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAatt                              >  1:174873/1‑150 (MQ=255)
                                                                                                                       aGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAatt                              <  2:1349486/150‑1 (MQ=255)
                                                                                                                            taatcgATTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttt                         >  2:202653/6‑150 (MQ=38)
                                                                                                                            cTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGTATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttt                         <  1:686213/150‑1 (MQ=255)
                                                                                                                             tAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattatttta                        >  1:846153/1‑150 (MQ=255)
                                                                                                                              aaaGATTGTCGAGGCGGCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAACTACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttat                       <  2:228901/150‑1 (MQ=255)
                                                                                                                                 gATTGTAGAGGCATGTTCTGATGACGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattat                    >  1:303455/1‑150 (MQ=39)
                                                                                                                                        gagGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATc             >  1:1494365/1‑150 (MQ=255)
                                                                                                                                        gagGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATc             >  1:1610273/1‑150 (MQ=255)
                                                                                                                                          ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatttt                 >  2:15476/1‑144 (MQ=255)
                                                                                                                                          ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAattattttattatttt                 <  1:15476/144‑1 (MQ=255)
                                                                                                                                          ggCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCAc           <  1:461371/150‑1 (MQ=255)
                                                                                                                                               catcaGAACATGCCTCTACTATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGct      <  1:234874/150‑1 (MQ=255)
                                                                                                                                               catcaGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGct      >  1:1088610/1‑150 (MQ=255)
                                                                                                                                                atcaGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGctt     >  1:969003/1‑150 (MQ=255)
                                                                                                                                                  caGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGcttcc   <  2:247861/150‑1 (MQ=255)
                                                                                                                                                   aGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGcttcct  <  1:103627/150‑1 (MQ=255)
                                                                                                                                                   aGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGcttcct  <  1:550396/150‑1 (MQ=255)
                                                                                                                                                   aGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGcttcct  <  1:754594/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATAGTTAACATTTTATCTTATGACATACACCTCATGTTCGATGGGAAAATAATTATATTTTGCACAAATCATTCAGCCTCAAAGCATAATATGAAATTTCTTATCTCATAGCAGAGAAAGTCCCTAAAGATTGTAGAGGCGTCATCAGAACATGCCTCTACAATCGATTAAGAAAACCTATGAAATACTGGCAGGATTAAAAAGACATACCACCGTATTCATGGAGTTTTAATGTAGTTGAAAACAACTAAGCCCTGAGGAATAAATTATTTTATTATTTTTATCACGTGCTTCCT  >  NC_000913/3632623‑3632919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: