Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,647,672 G→C 6.2% intergenic (+21/+33) gor → / ← dinQ glutathione oxidoreductase/UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,647,6720GC 6.2% 314.2 / 7.4 93intergenic (+21/+33)gor/dinQglutathione oxidoreductase/UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (44/43);  new base C (3/3);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 102 total aligned reads displayed.

GGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCAT  >  NC_000913/3647523‑3647821
                                                                                                                                                     |                                                                                                                                                     
ggCTTTGGTATGGACGAAATGTTGCAGTGCTTCGCGGTGGCGCTGAAGATGGGGGCTACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGagtag                                                                                                                                                       <  2:1636677/150‑1 (MQ=255)
ggCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGagtag                                                                                                                                                       >  2:1017196/1‑150 (MQ=255)
 gCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGagtagt                                                                                                                                                      >  1:438927/1‑150 (MQ=255)
      ggTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGct                                                                                                                                                 <  1:56616/150‑1 (MQ=255)
       gTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                                <  1:189099/150‑1 (MQ=255)
       gTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                                >  2:1233570/1‑150 (MQ=255)
            gACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGc                                                                                                                                           >  1:534000/1‑150 (MQ=255)
            gACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGc                                                                                                                                           <  1:1230883/150‑1 (MQ=255)
             aCGAATTGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCATAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGCACACTACTCTTAGcc                                                                                                                                          <  1:305721/150‑1 (MQ=255)
             aCGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGcc                                                                                                                                          <  1:1639974/150‑1 (MQ=255)
              cGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGccc                                                                                                                                         >  2:194479/1‑150 (MQ=255)
              cGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGccc                                                                                                                                         <  1:227938/150‑1 (MQ=255)
              cGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGCACACTACTCTTAGccc                                                                                                                                         <  1:1451311/150‑1 (MQ=255)
               gAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGCACACTACTCTTAGCCCt                                                                                                                                        <  2:1185346/150‑1 (MQ=255)
                 aaTGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTa                                                                                                                                      <  1:1441076/150‑1 (MQ=255)
                 aaTGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTa                                                                                                                                      <  1:894030/150‑1 (MQ=255)
                       gCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACg                                                                                                                                <  2:61377/150‑1 (MQ=255)
                       gCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGAATTAGACAATACCGTCGCCATTCAGGCAACGGCGGCAGAAGAGTACGTGACAATGGGGTAAATGTTAAAGGGGAAAGAGTACTGTGCTCAAAGCCATTGAATAct                                                                                                                                >  2:1102831/1‑149 (MQ=255)
                           ggCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTc                                                                                                                            <  2:188571/150‑1 (MQ=255)
                              ttCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGc                                                                                                                         <  1:524298/150‑1 (MQ=255)
                              ttCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGc                                                                                                                         <  2:1580238/150‑1 (MQ=255)
                                cgcgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACGATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTa                                                                                                                       >  1:1704521/1‑150 (MQ=255)
                                 gcgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTAt                                                                                                                      <  1:471658/150‑1 (MQ=255)
                                  cgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACATTGGGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATc                                                                                                                     >  1:1256975/1‑150 (MQ=255)
                                  cgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATc                                                                                                                     <  1:1494066/150‑1 (MQ=255)
                                  cgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATc                                                                                                                     <  1:961240/150‑1 (MQ=255)
                                     tGGCGCTGAAGATGGGGGCAACCAAAAAATACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTTACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGt                                                                                                                  <  2:776181/150‑1 (MQ=255)
                                       gcgcTGAAGATGGGTGAAACCAAAAAAGTCTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGAGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTc                                                                                                                <  1:326372/150‑1 (MQ=255)
                                        cgcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCa                                                                                                               <  1:375247/150‑1 (MQ=255)
                                        cgcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCa                                                                                                               >  2:1167398/1‑150 (MQ=255)
                                        cgcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCa                                                                                                               >  2:1196084/1‑150 (MQ=255)
                                          cTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGa                                                                                                             >  2:1486433/1‑150 (MQ=255)
                                          cTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTAGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTACGCTATAAGTTCAGa                                                                                                             >  1:877325/1‑150 (MQ=255)
                                            gAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAg                                                                                                           >  2:1340263/1‑150 (MQ=255)
                                              aGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCt                                                                                                         >  2:434982/1‑150 (MQ=255)
                                              aGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCt                                                                                                         <  1:1017196/150‑1 (MQ=255)
                                               gATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTg                                                                                                        >  2:275497/1‑150 (MQ=255)
                                                aTGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGa                                                                                                       <  1:793115/150‑1 (MQ=255)
                                                   ggggCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACCATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGc                                                                                                    >  1:240220/1‑150 (MQ=255)
                                                     ggCAACCAAAAAAGAATTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGATAAATGTTAACGGGCTAAGAGTAGTGTGCTCTTAGCCCGTAATTACGTTTCCGCTATCAGTTAAGAAGCTgcagcac                                                                                                  >  1:1273734/1‑149 (MQ=255)
                                                         aCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAg                                                                                              >  2:156514/1‑150 (MQ=255)
                                                         aCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAg                                                                                              >  1:751584/1‑150 (MQ=255)
                                                               aaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATc                                                                                        >  2:161878/1‑150 (MQ=255)
                                                                 aGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGGTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAg                                                                                      >  2:1443814/1‑150 (MQ=255)
                                                                 aGACATCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAg                                                                                      <  2:1116310/150‑1 (MQ=255)
                                                                    cTTCGACAATACCTTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                                   <  1:9932/150‑1 (MQ=255)
                                                                    cTTCGACAATACCGTCGCCTTTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                                   >  2:1667076/1‑150 (MQ=255)
                                                                    cTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                                   <  1:631646/150‑1 (MQ=255)
                                                                      tCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCca                                                                                 <  1:275497/150‑1 (MQ=255)
                                                                         aaaaTACCGTCGCCATTCACCCAACGGCGGCATAAGAGTTCGTTACAATGCGTTAAATGTTAACGGGCTAAGCGTCGTGGGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCCGAAGCTGAAGCAGAAAGCGTTCAGTTCcagcag                                                                             <  2:1234385/148‑1 (MQ=255)
                                                                          cAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCcagcag                                                                             <  2:248445/150‑1 (MQ=255)
                                                                          aaaTACCGTCGCCATTCACCCAACGGCGTCAGAAGAGTTCGTGACAATGCGTTAACTGTTAAAGGGCTAAGAGTAGTGGGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCcagcag                                                                             <  1:871381/149‑1 (MQ=255)
                                                                              aCCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCa                                                                         <  1:615212/150‑1 (MQ=255)
                                                                               ccGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                        >  1:124432/1‑150 (MQ=255)
                                                                               ccGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                        >  2:1529550/1‑150 (MQ=255)
                                                                               ccGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                        <  1:1671160/150‑1 (MQ=255)
                                                                                 gTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTcc                                                                                  <  2:522366/138‑1 (MQ=255)
                                                                                 gTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTcc                                                                                  >  1:522366/1‑138 (MQ=255)
                                                                                    gCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAACCAGAAAGCGGAACAGTTCCAGCAGCGCAATTAAc                                                                   >  1:1251022/1‑150 (MQ=255)
                                                                                      cATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGc                                                                 <  1:194479/150‑1 (MQ=255)
                                                                                      cATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGc                                                                 >  1:1689467/1‑150 (MQ=255)
                                                                                        ttCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGccc                                                               >  1:1615853/1‑150 (MQ=255)
                                                                                           aCCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTa                                                            >  1:39872/1‑150 (MQ=255)
                                                                                             ccAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGa                                                          <  1:397621/150‑1 (MQ=255)
                                                                                             ccAACGGCGCCAGAAGAGTTCGTGACAATGCGTTAAATGTTAACGGGCTAAGCCTAGTCAGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAAGTAACGCCCCTAGa                                                          >  1:78480/1‑150 (MQ=255)
                                                                                               aaCGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAAc                                                        >  2:928550/1‑150 (MQ=255)
                                                                                                   gcttcAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGCGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTGCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACgatg                                                    <  2:72233/146‑1 (MQ=255)
                                                                                                     ggcAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACgatgat                                                  >  2:1305703/1‑150 (MQ=255)
                                                                                                         gaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCt                                              >  1:1438615/1‑150 (MQ=255)
                                                                                                         gaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCt                                              >  1:1182446/1‑150 (MQ=255)
                                                                                                           agagTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTACCGCCCCTAGAACGATGATTGCttt                                            >  2:1119362/1‑150 (MQ=255)
                                                                                                              tttCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTatc                                         <  1:968093/149‑1 (MQ=255)
                                                                                                                 cGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTatcaat                                      >  2:1188629/1‑150 (MQ=255)
                                                                                                                    gACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCTCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAc                                   <  2:438927/150‑1 (MQ=255)
                                                                                                                    gACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAc                                   >  2:1315543/1‑150 (MQ=255)
                                                                                                                    gACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAc                                   >  1:138144/1‑150 (MQ=255)
                                                                                                                       aaTGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCg                                <  2:216294/150‑1 (MQ=255)
                                                                                                                       aaTGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCg                                >  2:475784/1‑150 (MQ=255)
                                                                                                                             ttAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTtct                          <  2:1467501/150‑1 (MQ=255)
                                                                                                                               aaaTGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTcc                        <  1:434982/150‑1 (MQ=255)
                                                                                                                                     tAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGa                  >  1:574301/1‑150 (MQ=255)
                                                                                                                                      aaaGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAATGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGAt                 <  1:156514/150‑1 (MQ=255)
                                                                                                                                      aaaGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGAt                 <  1:1486433/150‑1 (MQ=255)
                                                                                                                                        aGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg               >  2:1483564/1‑150 (MQ=255)
                                                                                                                                        aGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg               <  2:1438615/150‑1 (MQ=255)
                                                                                                                                        aGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg               <  2:1182446/150‑1 (MQ=255)
                                                                                                                                        aGGGCTAAGAGCACACTACTCTTAGCCCTTAATTACGTTTCGGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg               >  2:661961/1‑150 (MQ=255)
                                                                                                                                        aGGGCTAAGAGCACACTACTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg               >  1:201363/1‑150 (MQ=255)
                                                                                                                                          ggCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg             <  1:1305703/150‑1 (MQ=255)
                                                                                                                                          ggCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg             >  2:1699055/1‑150 (MQ=255)
                                                                                                                                          ggCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg             <  2:124432/150‑1 (MQ=255)
                                                                                                                                           gCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGt            >  1:1013900/1‑150 (MQ=255)
                                                                                                                                            cTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTATCCATGCGATGGAGTg           >  1:474931/1‑150 (MQ=255)
                                                                                                                                              aaGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATAAATCACCCGTTTTCTCCATGCGATGGAGTgag         >  2:1661114/1‑150 (MQ=255)
                                                                                                                                                 agtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCNNAAGCTGANGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAt      >  1:1702994/1‑150 (MQ=255)
                                                                                                                                                 agtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAt      >  1:1709507/1‑150 (MQ=255)
                                                                                                                                                  gtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCATCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATg     <  2:1394548/150‑1 (MQ=255)
                                                                                                                                                    agtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCa   <  2:1704521/150‑1 (MQ=255)
                                                                                                                                                    agtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCa   <  2:1323886/150‑1 (MQ=255)
                                                                                                                                                     gtgtGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCAt  >  2:742584/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCAT  >  NC_000913/3647523‑3647821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: