Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,647,673 T→A 5.9% intergenic (+22/+32) gor → / ← dinQ glutathione oxidoreductase/UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,647,6730TA 5.9% 262.9 / 3.2 93intergenic (+22/+32)gor/dinQglutathione oxidoreductase/UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (44/43);  new base A (3/3);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATC  >  NC_000913/3647524‑3647822
                                                                                                                                                     |                                                                                                                                                     
gCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGagtagt                                                                                                                                                       >  1:438927/1‑150 (MQ=255)
     ggTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGct                                                                                                                                                  <  1:56616/150‑1 (MQ=255)
      gTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                                 <  1:189099/150‑1 (MQ=255)
      gTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                                 >  2:1233570/1‑150 (MQ=255)
           gACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGc                                                                                                                                            >  1:534000/1‑150 (MQ=255)
           gACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGc                                                                                                                                            <  1:1230883/150‑1 (MQ=255)
            aCGAATTGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCATAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGCACACTACTCTTAGcc                                                                                                                                           <  1:305721/150‑1 (MQ=255)
            aCGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGcc                                                                                                                                           <  1:1639974/150‑1 (MQ=255)
             cGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGccc                                                                                                                                          >  2:194479/1‑150 (MQ=255)
             cGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGccc                                                                                                                                          <  1:227938/150‑1 (MQ=255)
             cGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGCACACTACTCTTAGccc                                                                                                                                          <  1:1451311/150‑1 (MQ=255)
              gAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGCACACTACTCTTAGCCCt                                                                                                                                         <  2:1185346/150‑1 (MQ=255)
                aaTGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTa                                                                                                                                       <  1:1441076/150‑1 (MQ=255)
                aaTGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTa                                                                                                                                       <  1:894030/150‑1 (MQ=255)
                      gCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACg                                                                                                                                 <  2:61377/150‑1 (MQ=255)
                      gCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGAATTAGACAATACCGTCGCCATTCAGGCAACGGCGGCAGAAGAGTACGTGACAATGGGGTAAATGTTAAAGGGGAAAGAGTACTGTGCTCAAAGCCATTGAATAct                                                                                                                                 >  2:1102831/1‑149 (MQ=255)
                          ggCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTc                                                                                                                             <  2:188571/150‑1 (MQ=255)
                             ttCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGc                                                                                                                          <  1:524298/150‑1 (MQ=255)
                             ttCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGc                                                                                                                          <  2:1580238/150‑1 (MQ=255)
                               cgcgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACGATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTa                                                                                                                        >  1:1704521/1‑150 (MQ=255)
                                gcgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTAt                                                                                                                       <  1:471658/150‑1 (MQ=255)
                                 cgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACATTGGGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATc                                                                                                                      >  1:1256975/1‑150 (MQ=255)
                                 cgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATc                                                                                                                      <  1:1494066/150‑1 (MQ=255)
                                 cgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATc                                                                                                                      <  1:961240/150‑1 (MQ=255)
                                    tGGCGCTGAAGATGGGGGCAACCAAAAAATACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTTACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGt                                                                                                                   <  2:776181/150‑1 (MQ=255)
                                      gcgcTGAAGATGGGTGAAACCAAAAAAGTCTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGAGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTc                                                                                                                 <  1:326372/150‑1 (MQ=255)
                                       cgcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCa                                                                                                                >  2:1167398/1‑150 (MQ=255)
                                       cgcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCa                                                                                                                >  2:1196084/1‑150 (MQ=255)
                                       cgcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCa                                                                                                                <  1:375247/150‑1 (MQ=255)
                                         cTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGa                                                                                                              >  2:1486433/1‑150 (MQ=255)
                                         cTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTAGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTACGCTATAAGTTCAGa                                                                                                              >  1:877325/1‑150 (MQ=255)
                                           gAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAg                                                                                                            >  2:1340263/1‑150 (MQ=255)
                                             aGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCt                                                                                                          >  2:434982/1‑150 (MQ=255)
                                             aGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCt                                                                                                          <  1:1017196/150‑1 (MQ=255)
                                              gATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTg                                                                                                         >  2:275497/1‑150 (MQ=255)
                                               aTGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGa                                                                                                        <  1:793115/150‑1 (MQ=255)
                                                  ggggCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACCATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGc                                                                                                     >  1:240220/1‑150 (MQ=255)
                                                    ggCAACCAAAAAAGAATTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGATAAATGTTAACGGGCTAAGAGTAGTGTGCTCTTAGCCCGTAATTACGTTTCCGCTATCAGTTAAGAAGCTgcagcac                                                                                                   >  1:1273734/1‑149 (MQ=255)
                                                        aCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAg                                                                                               >  2:156514/1‑150 (MQ=255)
                                                        aCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAg                                                                                               >  1:751584/1‑150 (MQ=255)
                                                              aaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATc                                                                                         >  2:161878/1‑150 (MQ=255)
                                                                aGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGGTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAg                                                                                       >  2:1443814/1‑150 (MQ=255)
                                                                aGACATCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAg                                                                                       <  2:1116310/150‑1 (MQ=255)
                                                                   cTTCGACAATACCTTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                                    <  1:9932/150‑1 (MQ=255)
                                                                   cTTCGACAATACCGTCGCCTTTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                                    >  2:1667076/1‑150 (MQ=255)
                                                                   cTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                                    <  1:631646/150‑1 (MQ=255)
                                                                     tCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCca                                                                                  <  1:275497/150‑1 (MQ=255)
                                                                        aaaaTACCGTCGCCATTCACCCAACGGCGGCATAAGAGTTCGTTACAATGCGTTAAATGTTAACGGGCTAAGCGTCGTGGGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCCGAAGCTGAAGCAGAAAGCGTTCAGTTCcagcag                                                                              <  2:1234385/148‑1 (MQ=255)
                                                                         cAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCcagcag                                                                              <  2:248445/150‑1 (MQ=255)
                                                                         aaaTACCGTCGCCATTCACCCAACGGCGTCAGAAGAGTTCGTGACAATGCGTTAACTGTTAAAGGGCTAAGAGTAGTGGGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCcagcag                                                                              <  1:871381/149‑1 (MQ=255)
                                                                             aCCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCa                                                                          <  1:615212/150‑1 (MQ=255)
                                                                              ccGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                         >  2:1529550/1‑150 (MQ=255)
                                                                              ccGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                         >  1:124432/1‑150 (MQ=255)
                                                                              ccGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                         <  1:1671160/150‑1 (MQ=255)
                                                                                gTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTcc                                                                                   <  2:522366/138‑1 (MQ=255)
                                                                                gTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTcc                                                                                   >  1:522366/1‑138 (MQ=255)
                                                                                   gCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAACCAGAAAGCGGAACAGTTCCAGCAGCGCAATTAAc                                                                    >  1:1251022/1‑150 (MQ=255)
                                                                                     cATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGc                                                                  <  1:194479/150‑1 (MQ=255)
                                                                                     cATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGc                                                                  >  1:1689467/1‑150 (MQ=255)
                                                                                       ttCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGccc                                                                >  1:1615853/1‑150 (MQ=255)
                                                                                          aCCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTa                                                             >  1:39872/1‑150 (MQ=255)
                                                                                            ccAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGa                                                           <  1:397621/150‑1 (MQ=255)
                                                                                            ccAACGGCGCCAGAAGAGTTCGTGACAATGCGTTAAATGTTAACGGGCTAAGCCTAGTCAGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAAGTAACGCCCCTAGa                                                           >  1:78480/1‑150 (MQ=255)
                                                                                              aaCGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAAc                                                         >  2:928550/1‑150 (MQ=255)
                                                                                                  gcttcAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGCGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTGCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACgatg                                                     <  2:72233/146‑1 (MQ=255)
                                                                                                    ggcAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACgatgat                                                   >  2:1305703/1‑150 (MQ=255)
                                                                                                     gcAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGAtt                                                  >  1:884354/1‑150 (MQ=255)
                                                                                                        gaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCt                                               >  1:1182446/1‑150 (MQ=255)
                                                                                                        gaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCt                                               >  1:1438615/1‑150 (MQ=255)
                                                                                                          agagTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTACCGCCCCTAGAACGATGATTGCttt                                             >  2:1119362/1‑150 (MQ=255)
                                                                                                             tttCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTatc                                          <  1:968093/149‑1 (MQ=255)
                                                                                                                cGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTatcaat                                       >  2:1188629/1‑150 (MQ=255)
                                                                                                                   gACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCTCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAc                                    <  2:438927/150‑1 (MQ=255)
                                                                                                                   gACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAc                                    >  2:1315543/1‑150 (MQ=255)
                                                                                                                   gACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAc                                    >  1:138144/1‑150 (MQ=255)
                                                                                                                      aaTGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCg                                 >  2:475784/1‑150 (MQ=255)
                                                                                                                      aaTGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCg                                 <  2:216294/150‑1 (MQ=255)
                                                                                                                            ttAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTtct                           <  2:1467501/150‑1 (MQ=255)
                                                                                                                              aaaTGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTcc                         <  1:434982/150‑1 (MQ=255)
                                                                                                                                    tAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGa                   >  1:574301/1‑150 (MQ=255)
                                                                                                                                     aaaGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAATGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGAt                  <  1:156514/150‑1 (MQ=255)
                                                                                                                                     aaaGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGAt                  <  1:1486433/150‑1 (MQ=255)
                                                                                                                                       aGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg                <  2:1438615/150‑1 (MQ=255)
                                                                                                                                       aGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg                >  2:1483564/1‑150 (MQ=255)
                                                                                                                                       aGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg                <  2:1182446/150‑1 (MQ=255)
                                                                                                                                       aGGGCTAAGAGCACACTACTCTTAGCCCTTAATTACGTTTCGGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg                >  2:661961/1‑150 (MQ=255)
                                                                                                                                       aGGGCTAAGAGCACACTACTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgg                >  1:201363/1‑150 (MQ=255)
                                                                                                                                         ggCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg              <  2:124432/150‑1 (MQ=255)
                                                                                                                                         ggCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg              <  1:1305703/150‑1 (MQ=255)
                                                                                                                                         ggCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg              >  2:1699055/1‑150 (MQ=255)
                                                                                                                                          gCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGt             >  1:1013900/1‑150 (MQ=255)
                                                                                                                                           cTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTATCCATGCGATGGAGTg            >  1:474931/1‑150 (MQ=255)
                                                                                                                                             aaGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATAAATCACCCGTTTTCTCCATGCGATGGAGTgag          >  2:1661114/1‑150 (MQ=255)
                                                                                                                                                agtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCNNAAGCTGANGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAt       >  1:1702994/1‑150 (MQ=255)
                                                                                                                                                agtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAt       >  1:1709507/1‑150 (MQ=255)
                                                                                                                                                 gtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCATCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATg      <  2:1394548/150‑1 (MQ=255)
                                                                                                                                                   agtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCa    <  2:1704521/150‑1 (MQ=255)
                                                                                                                                                   agtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCa    <  2:1323886/150‑1 (MQ=255)
                                                                                                                                                    gtgtGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCAt   >  2:742584/1‑150 (MQ=255)
                                                                                                                                                     tgtgCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATCCGATGGAGTGAGAATGCATc  <  1:161878/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATC  >  NC_000913/3647524‑3647822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: