BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,648,128	G→T	5.2%	noncoding (66/84 nt)	agrA →	inactive antisense sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,128	0	G	T	5.2%	396.3 / 9.6	135	noncoding (66/84 nt)	agrA	inactive antisense sRNA
Reads supporting (aligned to +/- strand): ref base G (54/74); new base T (4/3); total (58/77)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-02

Only 100 of 137 total aligned reads displayed.

TAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGC  >  NC_000913/3647979‑3648277

tAACTGGATAATCATACAGTACATGAAGGTTATAAAACCAGCTCGTCCTTGCAATAGTTTCAGTCTTGTATTAGCTTTGATGCGTTAGTTGATGGGTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACg                                                                                                                                                       <  2:76401/150‑1 (MQ=38)

 aaCTGGAAAATCATACAGTACATGCAGGTTATAAAACCAGCCCGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGc                                                                                                                                                      >  1:1036044/1‑150 (MQ=255)

     ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTTGAAAGTAAAAAACCGACGCAAAg                                                                                                                                                  >  1:152285/1‑150 (MQ=255)

      gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGt                                                                                                                                                 <  1:141506/150‑1 (MQ=255)

      gATAATCATACAGTACATGCAGGGTATAAAAGCAGCACGTCCGTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGt                                                                                                                                                 <  1:1106889/150‑1 (MQ=255)

       aTAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTc                                                                                                                                                <  2:974889/150‑1 (MQ=255)

          aTCATACAGTACATGCAGGTTATAAAACGAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                                                                                                             <  1:1008222/150‑1 (MQ=255)

          aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                                                                                                             >  1:869698/1‑150 (MQ=255)

            cATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAGAAAACCGACGCAAAGTCGGttt                                                                                                                                           <  1:1550750/150‑1 (MQ=255)

               aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTTCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                        <  2:803902/150‑1 (MQ=255)

                cactACATGCAGGTTATACAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                       <  2:349376/147‑1 (MQ=255)

                cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                       <  1:631067/150‑1 (MQ=255)

                cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTGCAGTATGGTATTCGCTTTGATGCGTTAGATGATGGCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAACTCGGttttttt                                                                                                                                       <  1:1308250/150‑1 (MQ=37)

                  gTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                     >  2:1233783/1‑150 (MQ=255)

                      aTGCAGGTTTTAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATcc                                                                                                                                 <  1:582276/150‑1 (MQ=255)

                        gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCgg                                                                                                                               <  2:399159/150‑1 (MQ=255)

                         cAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGa                                                                                                                              >  1:1658161/1‑150 (MQ=255)

                           ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAtt                                                                                                                            >  1:541657/1‑150 (MQ=255)

                           ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAtt                                                                                                                            >  1:386771/1‑150 (MQ=255)

                            gTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCAGTTTTTTTACATCCGGATTc                                                                                                                           >  1:1553705/1‑150 (MQ=255)

                             ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                          >  2:1186320/1‑150 (MQ=255)

                              tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTAAATCCGGATTCgg                                                                                                                         >  2:749844/1‑150 (MQ=255)

                                 aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                      >  1:457930/1‑150 (MQ=255)

                                  aaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACaa                                                                                                                     >  1:1280085/1‑150 (MQ=255)

                                  aaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACaa                                                                                                                     >  2:257844/1‑150 (MQ=255)

                                    aCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATAAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGACACgg                                                                                                                   <  2:95621/150‑1 (MQ=255)

                                          aCGTCCTTGCAAAAGTTTCAGTATGGTAGTAGCATTGAGGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAACACCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAat                                                                                                             <  2:842027/150‑1 (MQ=25)

                                           cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                            >  1:1043325/1‑150 (MQ=255)

                                           cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                            <  1:458456/150‑1 (MQ=255)

                                           cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                            <  2:479907/150‑1 (MQ=255)

                                           cGTCCTTGCAATAGTTTCAGTATGGTATAAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                            <  1:290788/150‑1 (MQ=255)

                                               cTTGCAATAGTTTCAGTATGGTATTAGCATTGAGGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAc                                                                                                        <  1:850004/150‑1 (MQ=255)

                                                ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCGGATTCGGACATGCATTAAAATGaag                                                                                                       >  1:244910/1‑148 (MQ=21)

                                                  gCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGAt                                                                                                     <  2:742785/150‑1 (MQ=255)

                                                   cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGAAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                    >  2:663776/1‑150 (MQ=255)

                                                    aaTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGa                                                                                                   <  1:66867/150‑1 (MQ=255)

                                                     aTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCTGAAAAGTATTAATATGAAGATTAc                                                                                                  >  1:1412183/1‑150 (MQ=25)

                                                           tCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTg                                                                                            >  1:245534/1‑150 (MQ=255)

                                                             aGTTTGGTATAAGCATTGAGGCGTTAGATGAAGGCTATCTCACTCCATTCAGAGACACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaa                                                                                          <  2:814001/150‑1 (MQ=25)

                                                              gTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                         >  2:1649/1‑150 (MQ=255)

                                                                aTGGTATTAGCATTTATGCGTTATATGATGGCTATCTCACTCCAGTCAGTGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCACAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGt                                                                                       <  1:826963/150‑1 (MQ=255)

                                                                    tATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatat                                                                                   <  2:1718322/150‑1 (MQ=255)

                                                                     aTTATCATTGATGGGTTAGATTATTGCTATCTCACTCCATTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                  <  2:239774/150‑1 (MQ=25)

                                                                     aTTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                  <  2:461649/150‑1 (MQ=255)

                                                                     aTTAGCATTGATGCGTTAGATGATGGATATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                  <  1:432032/150‑1 (MQ=255)

                                                                       tAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttt                                                                                                                                           >  2:907245/1‑91 (MQ=255)

                                                                       tAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGttt                                                                                                                                           <  1:907245/91‑1 (MQ=255)

                                                                        aGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAAt                                                                               <  2:1036044/150‑1 (MQ=255)

                                                                              gATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCAc                                                                         >  1:606960/1‑150 (MQ=255)

                                                                                tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                       >  1:143786/1‑150 (MQ=255)

                                                                                tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCAGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACACAGTGAAAGTATATAAATCGTCACTg                                                                       <  2:1553705/150‑1 (MQ=255)

                                                                                 gctttAGATGATGGCTATCTCACTCCAGTCATAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGc                                                                      <  2:326386/147‑1 (MQ=255)

                                                                                    ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                   >  1:868836/1‑150 (MQ=255)

                                                                                     tAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGata                                                                  <  1:339786/150‑1 (MQ=255)

                                                                                       gatgatGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                        <  2:184790/78‑1 (MQ=255)

                                                                                       gatgatGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                        >  1:184790/1‑78 (MQ=255)

                                                                                          gatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAc                                                             >  1:866336/1‑150 (MQ=255)

                                                                                          gatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAc                                                             >  1:807226/1‑150 (MQ=255)

                                                                                          gatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAc                                                             >  1:1521385/1‑150 (MQ=255)

                                                                                           atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                            <  2:245534/150‑1 (MQ=255)

                                                                                             ggCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGa                                                          <  1:518568/150‑1 (MQ=255)

                                                                                               cTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                        <  2:1043325/150‑1 (MQ=255)

                                                                                               cTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                        <  2:219776/150‑1 (MQ=255)

                                                                                                tATCTCACTCCAGTCAGAGCCACCAACTCATGGCTGGAAAGTAAAAAACCGACGCAAAGTAGTTTTTTTTACATCCGGATTCGGACAAGTCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCAGTGCGATATATACCGAAGt                                                       >  2:1287382/1‑150 (MQ=37)

                                                                                                 aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                      <  2:541657/150‑1 (MQ=255)

                                                                                                 aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                      <  2:1399933/150‑1 (MQ=255)

                                                                                                 aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                      >  1:1108610/1‑150 (MQ=255)

                                                                                                     cACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctcc                                                  >  2:1416172/1‑150 (MQ=255)

                                                                                                      aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccc                                                 <  2:1195387/150‑1 (MQ=255)

                                                                                                          cAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                             >  2:1594133/1‑150 (MQ=255)

                                                                                                          cAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGCTGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                             <  1:903775/150‑1 (MQ=255)

                                                                                                                    caccaACTCAGGGCTGGAAAGTAAAAAACCGCCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                   >  1:1522888/1‑150 (MQ=255)

                                                                                                                     accaACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaaga                                  <  2:1521385/150‑1 (MQ=255)

                                                                                                                      ccaACTCACGGCTGTAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaagaa                                 <  1:663776/150‑1 (MQ=255)

                                                                                                                         aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAATCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATc                              <  1:1266313/150‑1 (MQ=255)

                                                                                                                              gggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTaa                         >  1:126928/1‑150 (MQ=255)

                                                                                                                                gCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGATCCCTCCGCCAGCTGAAGAAATCGCTAAtt                       >  2:1650837/1‑150 (MQ=255)

                                                                                                                                   ggAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                             <  2:409487/141‑1 (MQ=255)

                                                                                                                                   ggAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                             >  1:409487/1‑141 (MQ=255)

                                                                                                                                   ggAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                    >  2:959673/1‑150 (MQ=255)

                                                                                                                                   ggAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                    >  1:1125033/1‑150 (MQ=255)

                                                                                                                                    gatgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                   >  1:1289487/7‑150 (MQ=255)

                                                                                                                                    gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                   <  2:152285/150‑1 (MQ=255)

                                                                                                                                    gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                   <  1:743806/150‑1 (MQ=255)

                                                                                                                                     aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                  <  1:332047/150‑1 (MQ=255)

                                                                                                                                     aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                  <  2:866336/150‑1 (MQ=255)

                                                                                                                                      tgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                 >  1:765798/5‑150 (MQ=255)

                                                                                                                                      aaGTAAAAAAACGACGAAAAGTTGGTTTTTTTACATCAGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                 <  1:285229/150‑1 (MQ=255)

                                                                                                                                       aGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCTCTAATTCTTGCaa                >  2:1113560/1‑150 (MQ=255)

                                                                                                                                         tAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCATCCGCCATCTGAAGAACTCGCTAATACTTGCAATg              >  1:320471/1‑150 (MQ=255)

                                                                                                                                         aaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATg              >  2:1302715/2‑150 (MQ=255)

                                                                                                                                            aaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTa           >  2:580378/1‑150 (MQ=255)

                                                                                                                                             aaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAg          >  2:989627/1‑150 (MQ=255)

                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGc         >  2:763902/1‑150 (MQ=255)

                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGc         <  2:868836/150‑1 (MQ=255)

                                                                                                                                                  gACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt     <  1:257844/150‑1 (MQ=255)

                                                                                                                                                  gACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt     >  1:383927/1‑150 (MQ=255)

                                                                                                                                                    cGCAAAGTCTGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg   <  1:689434/150‑1 (MQ=255)

                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg   <  1:1649/150‑1 (MQ=255)

                                                                                                                                                     gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATAGTCACTGCGATATATACCGAAGTGCTCCCTCCTCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGACACTGGc  >  1:917005/1‑150 (MQ=255)

TAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGC  >  NC_000913/3647979‑3648277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`