Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,648,359 G→T 6.8% noncoding (66/84 nt) agrB → antisense sRNA antitoxin for the DinQ toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,648,3590GT 6.8% 395.9 / 11.5 142noncoding (66/84 nt)agrBantisense sRNA antitoxin for the DinQ toxin
Reads supporting (aligned to +/- strand):  ref base G (72/59);  new base T (6/4);  total (79/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

Only 100 of 143 total aligned reads displayed.

GATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACC  >  NC_000913/3648210‑3648506
                                                                                                                                                     |                                                                                                                                                   
gATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACg                                                                                                                                                     >  1:731561/1‑150 (MQ=255)
   atataCCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTATTTATTGCAATGTTAGACACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTATCTCACTACAGACAGAGCCACCAACTCAGGGATGGAAAGTAAAAAACCGACGCaa                                                                                                                                                  >  1:1687406/1‑150 (MQ=38)
                ctccctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACTTTGCGTCGGtttttta                                                                                                                                     <  1:959673/150‑2 (MQ=38)
                  ccctccGCGAGCTGAAGAAATCGCTAAATCTTGCAATGTTAGCCACTTGCTAATAGTATTGAGCTGTTAGATAAGATCTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACTTTGCGTCGGttttttact                                                                                                                                   <  2:309857/150‑4 (MQ=37)
                   cctccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACg                                                                                                                                  >  1:1154217/1‑150 (MQ=255)
                      ccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTcc                                                                                                                               >  2:1150567/1‑150 (MQ=255)
                      ccGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTcc                                                                                                                               >  2:739381/1‑150 (MQ=255)
                         ccAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGa                                                                                                                            <  1:1648119/150‑1 (MQ=255)
                                 agaaATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcc                                                                                                                    >  2:1719459/1‑150 (MQ=255)
                                  gaaATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcct                                                                                                                   <  1:1601602/150‑1 (MQ=255)
                                  gaaATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTATCTAACTCCAGCCAGAGCCACCAACTCAGGTCTGGAAAGTAAAAAACCGAAGAAAAGACGGTTTTTTTACGTCATGATTAAGAcaa                                                                                                                   >  1:731424/1‑148 (MQ=255)
                                     aTCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAcctcct                                                                                                                <  2:662407/150‑1 (MQ=255)
                                       tgCTAATTCTTGCAATATTATCACCTTGGTAATAGTATTGATGTGTTAGATCATCTCTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCttt                                                                                                              <  1:937325/149‑1 (MQ=17)
                                        gCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGTCGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTc                                                                                                             >  2:1036031/1‑150 (MQ=255)
                                        gCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTc                                                                                                             >  2:540339/1‑150 (MQ=255)
                                        gCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTc                                                                                                             <  1:665794/150‑1 (MQ=255)
                                        gCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTc                                                                                                             <  1:1592219/150‑1 (MQ=255)
                                         cTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCAATCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGTTTTTTGTACGTCCTGAGTCAGACCTCCTTTCa                                                                                                            >  1:253187/1‑150 (MQ=255)
                                          tAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCaa                                                                                                           >  1:1580517/1‑150 (MQ=255)
                                            tttCTTGCAATCTTATCCAATGTCTAATAGTATTGAGGGGTTAGATCATTCCTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCGGGAAAGTAAAAAACCGACGCAAAGGCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                                         <  1:1485465/149‑1 (MQ=18)
                                            aTTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGTCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAAt                                                                                                         <  1:1157972/150‑1 (MQ=255)
                                               cTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGaa                                                                                                      >  2:47656/1‑150 (MQ=255)
                                               cTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGaa                                                                                                      >  1:823757/1‑150 (MQ=255)
                                               cTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGaa                                                                                                      <  1:47656/150‑1 (MQ=255)
                                                 tGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATa                                                                                                    >  1:1135118/1‑150 (MQ=255)
                                                   cAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGc                                                                                                  >  1:860025/1‑150 (MQ=255)
                                                   cAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGc                                                                                                  >  1:1353880/1‑150 (MQ=255)
                                                    actattAGCCAGTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGcc                                                                                                 >  2:1676570/5‑150 (MQ=255)
                                                         tAGCCACTGGATAATAGTTTAGAGCTGAAAGATAAGAACTCTCTTACTCCAGCCAGATCCAACAACTCAGGGTTGGAAAGTTAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTc                                                                                            <  2:269391/150‑1 (MQ=25)
                                                          aGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCa                                                                                           >  1:411740/1‑150 (MQ=255)
                                                          aGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCa                                                                                           <  1:1278354/150‑1 (MQ=255)
                                                           gCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCATAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCaa                                                                                          >  1:1129720/1‑150 (MQ=255)
                                                              aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAAt                                                                                       <  2:54578/150‑1 (MQ=255)
                                                              aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAAt                                                                                       <  2:1289487/150‑1 (MQ=255)
                                                              aCTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAAt                                                                                       <  2:1328715/150‑1 (MQ=255)
                                                                 gtcTATTAGTATTGAGCTGTTAGATTAGTTCTCTCTCACTCCAGGGAGAGCCACCAACTCAGGGGTTGAAAGTAAAAAACCGACGCAATGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAATATTca                                                                                    <  1:754132/148‑1 (MQ=21)
                                                                  gCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTcac                                                                                   <  2:823757/150‑1 (MQ=255)
                                                                     aaTAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACAACAGAAAGTCGGTTTTTTTTCGTCCAGAATCACTCCTCCGTTCAAAAGTATATCCAACTCAAAATTCCCAcca                                                                               >  2:1037329/1‑149 (MQ=21)
                                                                       tAGTATTGGGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTTGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTa                                                                              <  1:361696/150‑1 (MQ=255)
                                                                          tATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTa                                                                           <  2:217813/150‑1 (MQ=255)
                                                                           aTTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTAc                                                                          <  1:1150567/150‑1 (MQ=255)
                                                                           aTTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTAc                                                                          >  2:691345/1‑150 (MQ=255)
                                                                            ttGAGCTGTTAGATAAGAACTCTCTCACTCCCGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGCCCGCATTTCAAATGATTAGCCAACTCAAAATTCACACCTATTAca                                                                         >  1:286774/1‑149 (MQ=25)
                                                                             tGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAATTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCt                                                                        >  1:395854/1‑150 (MQ=255)
                                                                              gAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCtt                                                                       >  2:1386221/1‑150 (MQ=255)
                                                                                gCTGTTAGATATGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTcc                                                                     <  1:230281/150‑1 (MQ=255)
                                                                                gCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTcc                                                                     <  1:691345/150‑1 (MQ=255)
                                                                                 cTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCct                                                                    >  1:1043033/1‑150 (MQ=255)
                                                                                     tAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGc                                                                >  1:805880/1‑150 (MQ=255)
                                                                                      aGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACCCCTATTACCTTCCTCTGCa                                                               >  1:1670012/1‑150 (MQ=255)
                                                                                       gATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCAc                                                              >  1:707381/1‑150 (MQ=255)
                                                                                        aTAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACt                                                             <  2:765798/150‑1 (MQ=255)
                                                                                        aTAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACt                                                             >  2:790340/1‑150 (MQ=255)
                                                                                        aTAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACt                                                             <  2:860025/150‑1 (MQ=255)
                                                                                        aTAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACt                                                             <  1:1081918/150‑1 (MQ=255)
                                                                                           aGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAATAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAATTAATTGCCAACGCAAAATTGACCCCTATTTCCTTACTCTGCACTTtca                                                         >  1:765689/1‑150 (MQ=21)
                                                                                               ctctctCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACAt                                                      >  1:830165/1‑150 (MQ=255)
                                                                                                 ctctcACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCATATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTGTGCACTTACTCATTc                                                    <  2:396773/150‑1 (MQ=255)
                                                                                                 ctctcACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTc                                                    >  2:947439/1‑150 (MQ=255)
                                                                                                 ctctcACTAAAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGCTTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTc                                                    <  1:121492/150‑1 (MQ=255)
                                                                                                  tctcACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTATCAAATGAATAGCCAACTCAAAATTAACACCTATTACCTTCCTCTGCACTTACAAATTCg                                                   >  1:1492053/1‑150 (MQ=255)
                                                                                                   ctcACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGt                                                  <  2:1135118/150‑1 (MQ=255)
                                                                                                       cTCCAGCCAGAGACACCAACTCAGGGCTGGAAAGAAAAAAACCGACGCAAAGTCGGTTTTTTTACTTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTATGCACTTACACATTCGTTAAg                                              >  2:619580/1‑150 (MQ=255)
                                                                                                       atCCAGCCAGAGCAACCAACTCAGTGCTGGAAAGTAAAAAACCGACGCAAATTCGGTTTTTTTACGTCCTGATTCAGACGTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAg                                              <  2:731424/149‑1 (MQ=255)
                                                                                                         ccagccagAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAAGAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTc                                            >  1:140793/1‑150 (MQ=255)
                                                                                                         ccagccagAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCGTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTc                                            >  1:1314083/1‑150 (MQ=255)
                                                                                                          cagccagAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCa                                           >  2:1277415/1‑150 (MQ=255)
                                                                                                            gccagAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTGAGCCCTCCTTTCAAATGAATAGCCAAATCAAAATTCACACCTATTACCTACCGCTGCACTTACACATTGGTTAAGTCata                                         >  2:269553/1‑150 (MQ=37)
                                                                                                            gccagAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCata                                         <  1:1386221/150‑1 (MQ=255)
                                                                                                              cagAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAACTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACAATCGTAAAGTAatata                                       >  1:12156/1‑150 (MQ=255)
                                                                                                                  gCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGtt                                   >  1:1107298/1‑150 (MQ=255)
                                                                                                                  gCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTACCTCTGCACTTACACATTCGTTAAGTCAAATATgat                                   >  2:422060/1‑148 (MQ=255)
                                                                                                                       caACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTGCGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGa                              <  1:1430552/150‑1 (MQ=255)
                                                                                                                        aaCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGAc                             >  2:1090715/1‑150 (MQ=255)
                                                                                                                         aCTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACt                            <  1:1390728/150‑1 (MQ=255)
                                                                                                                          cTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATGCAGACCTCCTTGCAAATGAATAGCCAACTCAAAATTCACCCCTATTACCTTCCTCTGCACTTACCCATTCGTTAAGTCATATATGTTTTTGACtt                           >  2:426917/1‑150 (MQ=255)
                                                                                                                            cAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTAt                         >  2:906259/1‑150 (MQ=255)
                                                                                                                             agtgCTGGAAAGTAAAAAAGCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATc                        <  2:12156/147‑1 (MQ=255)
                                                                                                                              gggCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATcc                       <  1:967088/150‑1 (MQ=255)
                                                                                                                                 cTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCAAACCTATTACCTTCCTCAGCAATAACAAATTCGATAAGTCATATATGTTTTTGACTTATCCGCt                    >  1:291480/1‑150 (MQ=38)
                                                                                                                                 cTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTATCAAATGAATAGCCAACTCAAAATTCACACCTATTACATTCCTCTCCACTTACACAGTCGTTAAGTCATATATGTTTTTGACTTATCCGCt                    >  2:73214/1‑150 (MQ=255)
                                                                                                                                   ggAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTc                  <  2:1154217/150‑1 (MQ=255)
                                                                                                                                    gacgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCg                 >  2:1357214/7‑150 (MQ=255)
                                                                                                                                    gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCg                 >  2:1642389/1‑150 (MQ=255)
                                                                                                                                    gAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCg                 <  2:1534102/150‑1 (MQ=255)
                                                                                                                                     aaaGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGa                >  1:1059704/1‑150 (MQ=255)
                                                                                                                                       aGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAag              <  2:395854/150‑1 (MQ=255)
                                                                                                                                        gTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGAGATCCTTTCAAATGAATAGCCAACTCAAAATTAACACCTATTTCCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAaga             >  1:841705/1‑150 (MQ=255)
                                                                                                                                        gTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAaga             >  2:610681/1‑150 (MQ=255)
                                                                                                                                         tAAATAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGATTAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagag            <  2:1560071/150‑1 (MQ=255)
                                                                                                                                         tAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCATTCAAATGAATAACAAACTCAAAATTCACACCTATTGCCTTCCTCTGCACTTAAACATTCGTTAAGTCATATATGTTTTTGCCTTATGCGCTTCGAagag            >  2:1175456/1‑150 (MQ=39)
                                                                                                                                         aaaaaaaCCGACTTTGCGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagag            >  1:1649561/2‑150 (MQ=255)
                                                                                                                                          aaaaaaCCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagaga           <  2:101255/150‑1 (MQ=255)
                                                                                                                                          aaaaaaCCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAagaga           <  1:678974/150‑1 (MQ=255)
                                                                                                                                          aaaaaaCCGACGCAAAGTCGGTTTTGTGACGTCCTGAGTCAGCCCTCCTGTCAAATGAATAGCCCCCGCAAACTTCACACCTATTACCTTCCGCTGCACTTACACCTTCGGTAAGGCAGATATGTTTTTGACGTCTCCGCTGCGAagagc           >  1:938206/1‑149 (MQ=255)
                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGaca       <  1:540339/150‑1 (MQ=255)
                                                                                                                                               aCCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCGTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGacac      >  2:1494509/1‑150 (MQ=255)
                                                                                                                                                 cGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACGTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTa    >  1:855455/1‑150 (MQ=255)
                                                                                                                                                  gACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTAc   >  1:1101113/1‑150 (MQ=255)
                                                                                                                                                   aCGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTAcc  >  1:572554/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCTAATAGTATTGAGCTGTTAGATAAGAACTCTCTCACTCCAGCCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACCTCCTTTCAAATGAATAGCCAACTCAAAATTCACACCTATTACCTTCCTCTGCACTTACACATTCGTTAAGTCATATATGTTTTTGACTTATCCGCTTCGAAGAGAGACACTACC  >  NC_000913/3648210‑3648506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: