Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,654,553 C→G 5.3% intergenic (+26/‑130) slp → / → dctR outer membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,654,5530CG 5.3% 523.9 / 14.2 152intergenic (+26/‑130)slp/dctRouter membrane lipoprotein/Putative LuxR family repressor for dicarboxylate transport
Reads supporting (aligned to +/- strand):  ref base C (71/73);  new base G (3/5);  total (74/78)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01

Only 100 of 159 total aligned reads displayed.

AGTGGTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAG  >  NC_000913/3654404‑3654702
                                                                                                                                                     |                                                                                                                                                     
 gTGGTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTtct                                                                                                                                                      <  1:325151/150‑1 (MQ=255)
  tGGTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTtctc                                                                                                                                                     <  2:1571640/150‑1 (MQ=255)
  gggTTAATACCACCTATAACCTGTGGGATTACGGCTATGGGGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTtctc                                                                                                                                                     <  2:1133131/149‑1 (MQ=255)
   ggTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCa                                                                                                                                                    >  1:330727/1‑150 (MQ=255)
    gTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGGGCCTTAATACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAg                                                                                                                                                   <  1:1682533/150‑1 (MQ=255)
      tAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCa                                                                                                                                                 <  1:193322/150‑1 (MQ=255)
      tAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCa                                                                                                                                                 >  2:1335754/1‑150 (MQ=255)
       aaTACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCAt                                                                                                                                                >  2:1118678/1‑150 (MQ=255)
         tACCACCTATAACCTGTGGGATTATGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCt                                                                                                                                              <  1:1240181/150‑1 (MQ=255)
         tACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCt                                                                                                                                              >  1:709730/1‑150 (MQ=255)
          accaccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTg                                                                                                                                             >  1:1622624/1‑150 (MQ=255)
          accacaTATTTCGTGGGGGATTACGGCCATCGTGCATGCTGGCCGGATCCGTGCTGGGGTGCGCGTTACTTCACGAACTCGGTGATTCCGGTAACTCCTGAGCTGGTCATATAACACCACCGAAATATGCAGATGCTTTCTCAGCATCTg                                                                                                                                             <  2:279738/150‑1 (MQ=255)
           ccaccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGc                                                                                                                                            <  2:815623/150‑1 (MQ=255)
            caccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGca                                                                                                                                           <  1:1315153/150‑1 (MQ=255)
            caccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGca                                                                                                                                           <  2:1124464/150‑1 (MQ=255)
              ccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGcatc                                                                                                                                         >  2:1395017/1‑150 (MQ=255)
              ccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGcatc                                                                                                                                         <  1:981908/150‑1 (MQ=255)
              ccTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGGGCGCATTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGcatc                                                                                                                                         <  1:102508/150‑1 (MQ=255)
                  tacccTGTGGGATTACGGCGCGGGTGCCTTCTGGCCGGACCCGGGCTGGGGTGCGCCTGACTCCACCACTGCGGTGAGTCAGGTAACACCTGAGCTGGGCATATCACACCACCGAACGATGCAGATGCTTTCTCAGCAGCTGCATCATGc                                                                                                                                     <  1:67092/147‑1 (MQ=255)
                  taACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCGGGGGTGCGACTTACTACACCAATGCGGTGAGTCAGGTAACTCCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGc                                                                                                                                     <  2:1099630/150‑1 (MQ=255)
                    aCCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCAt                                                                                                                                   >  2:1023523/1‑150 (MQ=255)
                    aCCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCAt                                                                                                                                   >  2:931392/1‑150 (MQ=255)
                    aCCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCAt                                                                                                                                   >  1:1063940/1‑150 (MQ=255)
                      cTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTa                                                                                                                                 <  1:1131947/150‑1 (MQ=255)
                       tgtgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACCCCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTAc                                                                                                                                >  1:1491060/1‑150 (MQ=255)
                       tgtgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTAc                                                                                                                                >  2:867871/1‑150 (MQ=255)
                         tgGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACaa                                                                                                                              >  1:1167564/1‑149 (MQ=255)
                             aTTACGGCTTTGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCaaa                                                                                                                          <  2:310443/150‑1 (MQ=255)
                               tACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAAtt                                                                                                                        <  2:397136/150‑1 (MQ=255)
                                 cGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTaa                                                                                                                      >  2:1662909/1‑150 (MQ=255)
                                  ggCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAAt                                                                                                                     <  1:3458/150‑1 (MQ=255)
                                   gCTATGGTGCATTCTGGCCGGAACCGGGCGGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCTTGCATTACATCAAATTAATa                                                                                                                    <  2:496165/150‑1 (MQ=255)
                                      aTGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATacac                                                                                                                 <  1:565943/150‑1 (MQ=255)
                                       tGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATAcaca                                                                                                                <  1:799318/150‑1 (MQ=255)
                                          tGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTa                                                                                                             >  1:572111/1‑150 (MQ=255)
                                           gCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTaa                                                                                                            >  1:242336/1‑150 (MQ=255)
                                                cTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTaa                                                                                                       <  1:1200355/150‑1 (MQ=255)
                                                cTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCAATACATCAAATTAATAAACAGTAAGCTaa                                                                                                       >  1:1475325/1‑150 (MQ=255)
                                                   gCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACAGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACta                                                                                                    >  2:1595759/1‑150 (MQ=255)
                                                             ggCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACtattattattat                                                                                          >  1:273158/1‑150 (MQ=255)
                                                                   ggTGCGCCTTACTACCCCAATGCGGTGAGTCAGGTAACACCTGAGCTGTGCAAATAACACCACCGAAAGATGCAGCTGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGccc                                                                                    <  1:501850/150‑1 (MQ=255)
                                                                   ggTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGccc                                                                                    >  2:186006/1‑150 (MQ=255)
                                                                   ggTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCAAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGccc                                                                                    >  2:1597985/1‑150 (MQ=255)
                                                                     tGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctg                                                                                  >  1:1665972/1‑150 (MQ=255)
                                                                      gcgcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgt                                                                                 >  2:942451/1‑150 (MQ=255)
                                                                       cgcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCcctgtc                                                                                >  2:1487039/1‑150 (MQ=255)
                                                                        gcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGcatc                                                                                                                                         >  1:1676808/1‑92 (MQ=38)
                                                                        gcCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTGAGAAAGCATCTGcatc                                                                                                                                         <  2:1676808/92‑1 (MQ=38)
                                                                           ttACTACACCAATGCGGGGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATCCAGATGCTTTCTCAGCATCTGCATCATGCATAACATCAAATAAATACAAAGTAAGCTAACTATTATTATTATAAGCcctgtcctgt                                                                            <  1:71357/150‑1 (MQ=255)
                                                                               tacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                        <  2:572111/150‑1 (MQ=255)
                                                                               tacACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAAt                                                                        <  1:1016630/150‑1 (MQ=255)
                                                                                   ccAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAcc                                                                    >  1:1685454/1‑150 (MQ=255)
                                                                                   ccAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTAcc                                                                    <  2:671587/150‑1 (MQ=255)
                                                                                    cAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCt                                                                   >  2:400574/1‑150 (MQ=255)
                                                                                     aaTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCtt                                                                  >  2:1203084/1‑150 (MQ=255)
                                                                                      aTGCGGTGAGTCAGGTAACACCTGATCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCttt                                                                 >  1:1039471/1‑150 (MQ=255)
                                                                                      aTGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCGTGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCttt                                                                 <  1:1541367/150‑1 (MQ=255)
                                                                                        gCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTgg                                                               >  1:1356664/1‑150 (MQ=255)
                                                                                         cGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGc                                                              <  2:709730/150‑1 (MQ=255)
                                                                                         cGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGc                                                              >  1:64106/1‑150 (MQ=255)
                                                                                             gAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAAc                                                          <  2:96227/150‑1 (MQ=255)
                                                                                                 cAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                      <  1:1186301/150‑1 (MQ=255)
                                                                                                 cAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAt                                                      >  2:904320/1‑150 (MQ=255)
                                                                                                  aGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGAtt                                                     >  2:608287/1‑150 (MQ=255)
                                                                                                    ctAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATTCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGACCAGTTAATTACCTTTGGCAAACTCATtat                                                   >  1:100542/2‑150 (MQ=255)
                                                                                                        cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATaaat                                               >  2:1203701/1‑149 (MQ=255)
                                                                                                        cacCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAg                                               >  2:1676228/1‑150 (MQ=255)
                                                                                                         acCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGt                                              <  2:594529/150‑1 (MQ=255)
                                                                                                         acCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGt                                              <  1:446384/150‑1 (MQ=255)
                                                                                                            tGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                           <  2:1039471/150‑1 (MQ=255)
                                                                                                            tGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTaa                                           >  2:1449734/1‑150 (MQ=255)
                                                                                                              aGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAAtt                                         >  1:399059/1‑149 (MQ=255)
                                                                                                              aGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATg                                         <  2:1631762/150‑1 (MQ=255)
                                                                                                               gCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGt                                        <  2:778543/150‑1 (MQ=255)
                                                                                                                 tGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAACTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTcc                                      >  1:643593/1‑150 (MQ=255)
                                                                                                                  ggTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTTTTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCg                                     >  2:245427/1‑150 (MQ=255)
                                                                                                                  ggTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCg                                     >  2:1296035/1‑150 (MQ=255)
                                                                                                                  ggTCAAATAACACCACCAAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCg                                     <  1:1597985/150‑1 (MQ=255)
                                                                                                                     cAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCAc                                  <  1:1133671/150‑1 (MQ=255)
                                                                                                                       aaTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCa                                <  1:94336/150‑1 (MQ=255)
                                                                                                                       aaTAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCa                                >  1:13496/1‑150 (MQ=255)
                                                                                                                        aTAACACCACAGAAATATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATAATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAg                               <  1:668080/150‑1 (MQ=255)
                                                                                                                          aaCACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATTTCCGCACAAGGa                             >  1:1409758/1‑150 (MQ=255)
                                                                                                                           acacCACCGAAAGATGCAGATGCTTTCTCAGCTTCTGCATCATGCATTACATAAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAg                            >  2:1720598/1‑150 (MQ=255)
                                                                                                                             accaccGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTc                          <  2:329902/150‑1 (MQ=255)
                                                                                                                              ccaccGAAAGATGCAGCTGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTTTTATAAGCCCTGTCCTGTTAATTACCTTTGGCAATCTGATTATAAAGTTAATGTGCGCACCAGGAGTCg                         >  2:1409697/1‑150 (MQ=255)
                                                                                                                              ccaccGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTACGCACCAGGAGTCg                         >  2:590234/1‑150 (MQ=255)
                                                                                                                                  cGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTa                     <  1:942451/150‑1 (MQ=255)
                                                                                                                                  cGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                    <  2:1412835/150‑1 (MQ=255)
                                                                                                                                   gAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTAt                    >  2:1611751/1‑150 (MQ=255)
                                                                                                                                    aaaGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATg                   <  1:608287/150‑1 (MQ=255)
                                                                                                                                      tgatgcagatgcTGAGAAAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGtt                 >  1:889690/2‑150 (MQ=255)
                                                                                                                                      agatgcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTTAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGtt                 <  1:1334694/150‑1 (MQ=255)
                                                                                                                                        atgcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTc               <  2:729545/150‑1 (MQ=255)
                                                                                                                                         tgcagatgcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCt              <  2:273158/150‑1 (MQ=255)
                                                                                                                                          gcagatgcTTTCTCAGCATCTGCATCATGCATTTCATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCtt             <  2:1076036/150‑1 (MQ=255)
                                                                                                                                                gcTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAAtt       <  1:1106143/150‑1 (MQ=255)
                                                                                                                                                 cTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTa      >  1:907405/1‑150 (MQ=255)
                                                                                                                                                   ttCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTAcc    <  1:1569615/150‑1 (MQ=255)
                                                                                                                                                    tctcAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCa   >  2:880865/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTGGTTAATACCACCTATAACCTGTGGGATTACGGCTATGGTGCATTCTGGCCGGAACCGGGCTGGGGTGCGCCTTACTACACCAATGCGGTGAGTCAGGTAACACCTGAGCTGGTCAAATAACACCACCGAAAGATGCAGATGCTTTCTCAGCATCTGCATCATGCATTACATCAAATTAATACACAGTAAGCTAACTATTATTATTATAAGCCCTGTCCTGTTAATTACCTTTGGCAAACTGATTATAAAGTTAATGTCCGCACCAGGAGTCGGTTATGTTTCTTATAATTACCAG  >  NC_000913/3654404‑3654702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: