Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,655,922 C→T 5.2% intergenic (‑20/+44) yhiD ← / ← hdeB putative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,9220CT 5.2% 427.5 / 5.4 133intergenic (‑20/+44)yhiD/hdeBputative Mg(2+) transport ATPase, inner membrane protein/acid‑resistance protein
Reads supporting (aligned to +/- strand):  ref base C (64/60);  new base T (4/3);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

GGCGCTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTT  >  NC_000913/3655774‑3656071
                                                                                                                                                    |                                                                                                                                                     
ggCGCTTCCCATACCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGagca                                                                                                                                                      <  2:664550/150‑1 (MQ=255)
   gcTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAgcagca                                                                                                                                                   >  1:1060701/1‑150 (MQ=255)
        ccATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATg                                                                                                                                              <  1:1341489/150‑1 (MQ=255)
        ccATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATg                                                                                                                                              >  1:1283747/1‑150 (MQ=255)
            gCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTc                                                                                                                                          <  2:121974/150‑1 (MQ=255)
             ccAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCa                                                                                                                                         >  2:1030797/1‑150 (MQ=255)
             ccAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCa                                                                                                                                         >  1:1426247/1‑150 (MQ=255)
              cAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTgagccat                                                                                                                                        >  2:531779/1‑144 (MQ=255)
              cAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCaa                                                                                                                                        >  2:1406571/1‑150 (MQ=255)
                  tAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGAGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTg                                                                                                                                    <  1:647986/150‑1 (MQ=255)
                    aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                                  <  1:523254/150‑1 (MQ=255)
                    aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                                  <  2:723412/150‑1 (MQ=255)
                    aTACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCt                                                                                                                                  <  2:997063/150‑1 (MQ=255)
                         tgtgtgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTcc                                                                                                                             <  2:1274382/150‑1 (MQ=255)
                         tgtgcgCGTAACCCTTCTACTTTGCCGCGCATTTGCATTTCCATGCCAATAGCGCCAAAGGCAATTGCCGCGAGTATAAGACTTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTcc                                                                                                                             <  2:259409/150‑1 (MQ=255)
                             tgCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCtttt                                                                                                                         <  2:1541748/150‑1 (MQ=38)
                              gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                        >  1:962054/1‑150 (MQ=255)
                              gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                        >  2:28689/1‑150 (MQ=255)
                              gCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCttttt                                                                                                                        <  1:1090967/150‑1 (MQ=255)
                              gCGTAACCCTGCTACTTTGCCGCGAATTTGCCTTTCAATGCCAATAGCGCCACAGGCAAATGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTAATATAAAAATGAAAAAAGGAGAAGCAAGATGGCTAAAATTGCTACTCCtttta                                                                                                                        >  2:226891/1‑149 (MQ=255)
                                gTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTAc                                                                                                                      <  1:964023/150‑1 (MQ=255)
                                   aCCCTGCTCCTTTGCCGCGCATTTTCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTg                                                                                                                   <  2:362628/150‑1 (MQ=255)
                                       tGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCAcc                                                                                                               <  1:1489737/150‑1 (MQ=255)
                                         cTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATTATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTc                                                                                                             >  2:915919/1‑150 (MQ=255)
                                         cTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTc                                                                                                             >  2:316842/1‑150 (MQ=255)
                                           ccTTTGCCGCGCATTTGCCTTTACATGCCAATAGCGCCCCAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAAATGGCTCAACTTGCTAATACATTTTACTTGCACCTCaa                                                                                                           >  2:661061/1‑149 (MQ=255)
                                               tGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGGGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAAt                                                                                                       >  1:1181432/1‑150 (MQ=255)
                                               tGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTACTTGCACCTCATTAAtt                                                                                                      >  2:395121/1‑150 (MQ=255)
                                                 cgcggcaattgcctgtggcgctattggcatggaaaggcaaatgcCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                                                                                     >  2:985903‑M2/45‑150 (MQ=255)
                                                 ccGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTc                                                                                                     >  1:1450679/1‑150 (MQ=255)
                                                   gcgcATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCgg                                                                                                   <  2:927735/150‑1 (MQ=255)
                                                   cggcaattgcctgtggcgctattggcatggaaaggcaaatgcCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCgg                                                                                                   >  1:1151739‑M2/43‑150 (MQ=255)
                                                    ggcaattgcctgtggcgctattggcatggaaaggcaaatgcCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGc                                                                                                  <  2:1151739‑M2/109‑1 (MQ=255)
                                                     GCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCTAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCa                                                                                                 >  1:1647497‑M1/1‑150 (MQ=255)
                                                      CATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCaa                                                                                                <  1:150707‑M1/150‑1 (MQ=255)
                                                       ATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                                                                               >  2:344492‑M1/1‑150 (MQ=255)
                                                       ATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAg                                                                                               >  1:1516742‑M1/1‑150 (MQ=255)
                                                         ttGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTc                                                                                             <  2:829236/150‑1 (MQ=255)
                                                          tGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCa                                                                                            >  2:392032/1‑150 (MQ=255)
                                                          tGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTACTTTTTACTTGCACCTCATTAATTCGGCAAGTCa                                                                                            >  2:1036804/1‑150 (MQ=255)
                                                            ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                          >  1:1285316/1‑150 (MQ=255)
                                                            ccTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCAtt                                                                                          >  2:1013247/1‑150 (MQ=255)
                                                             cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                         >  2:1183853/1‑150 (MQ=255)
                                                             cTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTa                                                                                         >  1:485427/1‑150 (MQ=255)
                                                              tttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAg                                                                                        <  1:931428/150‑1 (MQ=255)
                                                              ctgtggcgctattggcatggaaaggcaaatgCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTACTTTTTACTTTCACCTCTTTAATTCGGAAAGTCATtat                                                                                        >  1:310421‑M2/32‑149 (MQ=255)
                                                               ttCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGa                                                                                       <  2:29570/150‑1 (MQ=255)
                                                                    tGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCtt                                                                                  >  1:1359625/1‑150 (MQ=255)
                                                                          tAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGAtttt                                                                            <  1:1447448/150‑1 (MQ=255)
                                                                            gcgcCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTTGATGCTTGATTTTTg                                                                          >  1:1038057/1‑150 (MQ=255)
                                                                                cacaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAgg                                                                      >  2:1290793/1‑150 (MQ=255)
                                                                                 acaGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGt                                                                     >  1:579148/1‑150 (MQ=255)
                                                                                    ggCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtata                                                                  <  1:231976/150‑1 (MQ=255)
                                                                                    ggCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGtata                                                                  <  2:1446069/150‑1 (MQ=255)
                                                                                          tgcCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAtt                                                            >  2:447344‑M2/4‑150 (MQ=255)
                                                                                            cCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAtttt                                                          <  2:962054‑M2/149‑1 (MQ=255)
                                                                                            cCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAAtttt                                                          <  2:150707‑M2/149‑1 (MQ=255)
                                                                                                gAGTATAAGACGTATAATAAATTCTGATGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTg                                                      <  2:767694/150‑1 (MQ=255)
                                                                                                  gTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTgag                                                    >  1:769342/1‑148 (MQ=255)
                                                                                                  gTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCg                                                    <  2:1328195/150‑1 (MQ=255)
                                                                                                   tataATAAGTATAATAAATTCTGCTGTAATATATAAATGCAAAAAGGAGCAGCAAGATGTTTCAACTTGCTACTCCTTTTTACTTGCCCCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATAAAAATTTTTCTGCgg                                                   <  1:264795/150‑1 (MQ=255)
                                                                                                   tataAGACGTATAATAAATTCTGCTGTGATATAAAAATGCAAAAAGTAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCCGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCgg                                                   <  1:521200/150‑1 (MQ=255)
                                                                                                       aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                               >  1:1242355/1‑150 (MQ=255)
                                                                                                       aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                               >  1:1434627/1‑150 (MQ=255)
                                                                                                       aGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGttt                                               >  1:679941/1‑150 (MQ=255)
                                                                                                             ataataAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTa                                         <  2:485427/150‑1 (MQ=255)
                                                                                                                ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                      >  1:122584/1‑150 (MQ=255)
                                                                                                                ataaATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAg                                      <  1:616087/150‑1 (MQ=255)
                                                                                                                   aaTTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTAt                                   >  2:1380695/1‑150 (MQ=255)
                                                                                                                   aaTTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTAt                                   >  1:314335/1‑150 (MQ=255)
                                                                                                                    aTTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTAtt                                  >  1:535238/1‑150 (MQ=255)
                                                                                                                       ctgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGTTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGa                               >  1:1637512/1‑150 (MQ=255)
                                                                                                                        tgctgTCATATAAAAATTCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCATTTTTACTTGCACCTCATTAATTCGGCTAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                              <  1:19183/150‑1 (MQ=255)
                                                                                                                        tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTa                                                                                                         <  1:1324736/75‑1 (MQ=255)
                                                                                                                        tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                              <  2:1604801/150‑1 (MQ=255)
                                                                                                                        tgctgTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGAt                              <  1:447344/150‑1 (MQ=255)
                                                                                                                             tCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACtt                         <  2:1450679/150‑1 (MQ=255)
                                                                                                                               atatAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTa                       >  2:1304756/1‑150 (MQ=255)
                                                                                                                                tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAg                      >  2:615145/1‑150 (MQ=255)
                                                                                                                                tataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAgg                     <  1:395121/150‑1 (MQ=255)
                                                                                                                                 ttaAAAATTCAATAAGGAGAAGCTAGATGTCTCAACTTGCTATTCCTTTTTACTTTCACCTCATTAATTCGGCAAGTCTTTAGATGCTTTATTTTTGAAGGTCTACAAATTTTTCTGCGGTTTTTTCTTACAGTATTCGATCACTTTAgg                     <  1:1391257/149‑1 (MQ=255)
                                                                                                                                 ataAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAgg                     <  2:371539/150‑1 (MQ=255)
                                                                                                                                 ataAAAATGAAAAAAGGAGCAGCAAGATGGGTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAgg                     <  1:1626381/150‑1 (MQ=255)
                                                                                                                                      caagtaaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAAttt                >  1:765410/6‑150 (MQ=255)
                                                                                                                                      aaTGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAAttt                >  1:452031/1‑150 (MQ=255)
                                                                                                                                      aaTGCAAAAAGGAGCAGCAAGATGGATCAAGTTGCTACTCCATTTTACTTGCACCTCATTAATTCGGAAAGTCATTAGATGCATGATTTTTGAAGGTATACAAATTTTTCTGCGGGATTTTCTTACAGTATTAGCTCAATTTAGTAAttt                >  1:560706/1‑150 (MQ=255)
                                                                                                                                        tGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTCCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATGTTTGAAGGTAGACAAAGGTTTCTGCGGGTTTTTCGTCCAGTAGTCGATCACGTTCGGAATGtga              >  1:67390/1‑150 (MQ=255)
                                                                                                                                         gCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgag             <  2:1181432/150‑1 (MQ=255)
                                                                                                                                          cAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagt            >  1:806855/1‑150 (MQ=255)
                                                                                                                                          cAAAAAGGAGCAGCAAGATCGCTCAACTTGCTAATACTTTTTACATGCACCTCATTAATTCGGCAACTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGATTTTCTTACAGTATTGGATCACTTTAGGAATTtgagt            >  1:1183328/1‑150 (MQ=255)
                                                                                                                                           aaaatGGAGTAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg           <  2:1539849/150‑1 (MQ=255)
                                                                                                                                           aaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg           <  2:702939/150‑1 (MQ=255)
                                                                                                                                           aaaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgagtg           <  2:1285316/150‑1 (MQ=255)
                                                                                                                                            aaaaGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTtgtgtga          >  2:844742/1‑150 (MQ=255)
                                                                                                                                             aaaGGAGTAGCAAGTTGAGCCATCTTGCTGCTACTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTATGCGGGTTTTTCTTAAAGTATTCGATGACTTTAGGAATTtgagtgag         >  1:12378/1‑150 (MQ=38)
                                                                                                                                               aGGAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGAt       >  2:496208/1‑150 (MQ=39)
                                                                                                                                                ggAGTAGCAAGTTGAGCCATCTTGCTGCTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATTCAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATc      >  2:260225/1‑150 (MQ=38)
                                                                                                                                                ggAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATc      <  2:512186/150‑1 (MQ=255)
                                                                                                                                                  agcagcAAGATGGATAAACTTGCTACTCCTTTTAACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAACGTATACAAATATTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCgg    <  2:184862/150‑1 (MQ=255)
                                                                                                                                                    cagcaAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGtt  <  2:562699/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCGCTTCCCATGCCAATTAATACATGTGTGCGTAACCCTGCTCCTTTGCCGCGCATTTGCCTTTCCATGCCAATAGCGCCACAGGCAATTGCCGCGAGTATAAGACGTATAATAAATTCTGCTGTCATATAAAAATGCAAAAAGGAGCAGCAAGATGGCTCAACTTGCTACTCCTTTTTACTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTT  >  NC_000913/3655774‑3656071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: