Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,676,202 T→G 5.6% intergenic (+94/+88) yhjE → / ← yhjG putative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,676,2020TG 5.6% 384.1 / 10.3 125intergenic (+94/+88)yhjE/yhjGputative MFS transporter; membrane protein/putative inner membrane‑anchored periplasmic AsmA family protein
Reads supporting (aligned to +/- strand):  ref base T (71/47);  new base G (4/3);  total (75/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.75e-01

Only 100 of 126 total aligned reads displayed.

CTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGA  >  NC_000913/3676054‑3676351
                                                                                                                                                    |                                                                                                                                                     
ctTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                      <  1:587509/150‑1 (MQ=255)
ctTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTc                                                                                                                                                      <  1:995290/150‑1 (MQ=255)
 ttGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAGTTTCCATACAATATATTGAATTCGAAAGGATTTGTAGGCCGGATAAGGCGTTCa                                                                                                                                                     >  1:390742/1‑150 (MQ=255)
  tGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAATCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCTGATAAGGCGTTCAc                                                                                                                                                    >  1:329700/1‑150 (MQ=255)
   gACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                                   <  1:575738/150‑1 (MQ=255)
      gTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcgccg                                                                                                                                                <  2:968626/150‑1 (MQ=255)
           tCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                           <  1:429170/150‑1 (MQ=38)
           tCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                                   <  1:1273184/142‑1 (MQ=255)
           tCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCAcg                                                                                                                                                   >  2:1273184/1‑142 (MQ=255)
                ctgctgctgACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                                                                                                                      >  2:1602303/1‑150 (MQ=255)
                 tgctgctgACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATg                                                                                                                                     <  2:1676438/150‑1 (MQ=255)
                   ctgctgACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGaa                                                                                                                                   >  2:773154/1‑150 (MQ=255)
                    tgctgACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                                                                                                                                  >  1:1176393/1‑150 (MQ=255)
                    tgctgACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAc                                                                                                                                  >  2:571413/1‑150 (MQ=255)
                     gctgACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACa                                                                                                                                 <  2:1616189/150‑1 (MQ=255)
                        gACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAg                                                                                                                              >  1:1019285/1‑150 (MQ=255)
                         aCCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGc                                                                                                                             <  1:469363/150‑1 (MQ=255)
                           ccATGAGACGCGACATCAGGCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcac                                                                                                                           <  1:109614/150‑1 (MQ=255)
                            cATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGcaca                                                                                                                          >  2:262989/1‑150 (MQ=255)
                             aTGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCacac                                                                                                                         >  2:537618/1‑150 (MQ=255)
                                 gaCGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTg                                                                                                                     >  1:1125767/1‑150 (MQ=255)
                                  aCGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGc                                                                                                                    >  2:1333065/1‑150 (MQ=255)
                                   cgcgACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGcc                                                                                                                   >  2:56642/1‑150 (MQ=255)
                                   cgcgACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGcc                                                                                                                   >  2:202476/1‑150 (MQ=255)
                                   cgcgACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATATCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGcc                                                                                                                   <  2:1209127/150‑1 (MQ=255)
                                      gACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaac                                                                                                                <  1:743984/150‑1 (MQ=255)
                                      gACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATCGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaac                                                                                                                <  2:528678/150‑1 (MQ=255)
                                        cATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaacaa                                                                                                              >  1:865982/1‑150 (MQ=255)
                                        cATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaacaa                                                                                                              >  2:260136/1‑150 (MQ=255)
                                        cATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCcaacaa                                                                                                              >  2:263975/1‑150 (MQ=255)
                                         aTCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAAt                                                                                                             <  1:995048/150‑1 (MQ=255)
                                               cGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTaatc                                                                                                       <  2:117442/150‑3 (MQ=255)
                                                gTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATc                                                                                                      <  1:1340676/150‑1 (MQ=255)
                                                 ttGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCt                                                                                                     >  1:1615754/1‑150 (MQ=255)
                                                  tGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTg                                                                                                    >  1:116565/1‑150 (MQ=255)
                                                  tGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTg                                                                                                    >  1:1297804/1‑150 (MQ=255)
                                                   gTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCCACAATCTAAATCTGt                                                                                                   >  1:299276/1‑150 (MQ=255)
                                                    taataaGAGTGAAACGCCTGATGCGTTACGCTTTTCAGGGGTGAATTTCCATACAATATATTTAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGtt                                                                                                  <  2:1508055/150‑1 (MQ=25)
                                                        aaGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAAt                                                                                              >  2:498765/1‑150 (MQ=255)
                                                         agagTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAAtt                                                                                             >  1:1683310/1‑150 (MQ=255)
                                                          gagTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTg                                                                                            <  2:23530/150‑1 (MQ=255)
                                                            gTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGcc                                                                                          >  2:1344899/1‑150 (MQ=255)
                                                             tGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCg                                                                                         <  1:1030516/150‑1 (MQ=255)
                                                              gAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCgg                                                                                        >  2:1392590/1‑150 (MQ=255)
                                                              gAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCgg                                                                                        >  2:1651649/1‑150 (MQ=255)
                                                              gAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCgg                                                                                        >  2:555081/1‑150 (MQ=255)
                                                                aaaGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGAt                                                                                      <  2:1005640/150‑1 (MQ=255)
                                                                 aaGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATg                                                                                     <  2:1019285/150‑1 (MQ=255)
                                                                 aaGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATg                                                                                     <  2:865982/150‑1 (MQ=255)
                                                                 aaGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATg                                                                                     <  2:885502/150‑1 (MQ=255)
                                                                   gCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTc                                                                                   >  2:673459/1‑150 (MQ=255)
                                                                     cTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAg                                                                                 <  2:613180/150‑1 (MQ=255)
                                                                       gATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGAc                                                                               >  2:515313/1‑150 (MQ=255)
                                                                           cgcTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATcc                                                                           >  2:446839/1‑150 (MQ=255)
                                                                               aCGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCa                                                                       <  1:575746/150‑1 (MQ=255)
                                                                                cGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCaa                                                                      >  2:1078000/1‑150 (MQ=255)
                                                                                cGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCAACAAAGCACACTTTGCAAACAATCTAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAt                                                                     >  2:71112/1‑150 (MQ=255)
                                                                                 gCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCTGACATCCGGCAAt                                                                     <  1:1455105/150‑1 (MQ=255)
                                                                                       cAGGCCTGATTTTCCATACAATATATTGAATTCGAAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCAGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatcat                                                               <  2:390742/150‑1 (MQ=255)
                                                                                         ggCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAAGTAtcatca                                                             >  1:178965/1‑150 (MQ=255)
                                                                                           ccTGAATTTCCATACAATATATTGACTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAct                                                           <  1:450201/150‑1 (MQ=255)
                                                                                           ccTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCAct                                                           >  1:736274/1‑150 (MQ=255)
                                                                                             tGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTatcataaatta                                                         >  1:351305/1‑144 (MQ=255)
                                                                                                 tttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCa                                                     <  1:238131/150‑1 (MQ=255)
                                                                                                 tttCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCAAACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCa                                                     >  1:1226234/1‑150 (MQ=255)
                                                                                                   tCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATc                                                   >  1:771874/1‑150 (MQ=255)
                                                                                                   tCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATc                                                   >  1:1479021/1‑150 (MQ=255)
                                                                                                    ccATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCt                                                  >  1:1481754/1‑150 (MQ=255)
                                                                                                    ccATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCt                                                  <  1:263975/150‑1 (MQ=255)
                                                                                                    ccATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCt                                                  <  1:260136/150‑1 (MQ=255)
                                                                                                     cATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTg                                                 >  1:1484651/1‑150 (MQ=255)
                                                                                                     cATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTg                                                 <  2:1515493/150‑1 (MQ=255)
                                                                                                         cAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGAc                                             <  2:1176393/150‑1 (MQ=255)
                                                                                                         cAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGAc                                             >  1:1201306/1‑150 (MQ=255)
                                                                                                               atTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATc                                       >  1:1632010/1‑150 (MQ=255)
                                                                                                               atTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATc                                       >  1:920842/1‑150 (MQ=255)
                                                                                                                ttGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCg                                      <  1:773154/150‑1 (MQ=255)
                                                                                                                 tGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGt                                     >  2:628698/1‑150 (MQ=255)
                                                                                                                  gAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCATCAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGTGACAAAATCGTc                                    >  1:1180530/1‑150 (MQ=255)
                                                                                                                    aTTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCg                                  <  2:1125767/150‑1 (MQ=255)
                                                                                                                      tCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGac                                <  2:1488492/150‑1 (MQ=255)
                                                                                                                       cGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGaca                               >  1:5365/1‑150 (MQ=255)
                                                                                                                       cGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGaca                               >  1:252837/1‑150 (MQ=255)
                                                                                                                       cGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGAGACAAAATCGTCCGaca                               >  2:457175/1‑150 (MQ=255)
                                                                                                                       cGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTGTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACCTCCGGCAAGTCTCCTCACTTCTGCATCTGCGCCAAAATCGTCCGcca                               >  1:319139/1‑150 (MQ=37)
                                                                                                                         cAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACt                             >  2:1292859/1‑150 (MQ=255)
                                                                                                                            ggATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGAt                          >  1:89381/1‑150 (MQ=255)
                                                                                                                              aTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTa                        <  2:1256438/150‑1 (MQ=255)
                                                                                                                               tttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAg                       <  1:1333065/150‑1 (MQ=255)
                                                                                                                                   tAGGCCGGATAAGGCGTTCACTCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTc                   <  1:1398661/150‑1 (MQ=255)
                                                                                                                                    atgCCGGATGCGGCGTGAACGCCTTATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTcc                  >  2:1083812/3‑150 (MQ=39)
                                                                                                                                    aGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTcc                  >  1:1223752/1‑150 (MQ=255)
                                                                                                                                     ggCCGGATAAGGCGCTCACGCCGCATCCGGCTTGAACAAAGCACACTTTGCCAACACACAAAATCTGTTTAATTCCCGGATGTCAGCCAACCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTccc                 <  2:231934/150‑1 (MQ=255)
                                                                                                                                      gCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTcccc                <  2:5365/150‑1 (MQ=255)
                                                                                                                                         ggATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTc             >  1:590127/1‑150 (MQ=255)
                                                                                                                                           aTAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCgg           <  2:1115504/150‑1 (MQ=255)
                                                                                                                                            tgcggCGTGAACGCCTTATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGa          <  1:1083812/147‑1 (MQ=255)
                                                                                                                                              aGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAg        <  1:537618/150‑1 (MQ=255)
                                                                                                                                                 tgTTCATGCCGGATGCGGCGTGAACAAAGCACACATTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACATCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCACTTCGGAAGGTg     >  2:612515/2‑150 (MQ=255)
                                                                                                                                                  gTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTga    <  1:498765/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTGACGTTAATCGCCCTGCTGCTGACCCATGAGACGCGACATCAGTCGTTGTAATAAGAGTGAAAAGCCTGATGCGCTACGCTTATCAGGCCTGAATTTCCATACAATATATTGAATTCGCAAGGATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAACAAAGCACACTTTGCCAACAATCTAAATCTGTTTAATTGCCGGATGTCAGACATCCGGCAATTATCATCACTTCTTCATCTGCGACAAAATCGTCCGACACTGATTAGCCTCCCCTTCGGAAGGTGAGA  >  NC_000913/3676054‑3676351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: