Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,681,132 A→G 6.7% V270A (GTG→GCG) ‡ yhjJ ← putative periplasmic M16 family chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,681,1320AG 6.7% 324.5 / 11.6 122V270A (GTG→GCG) ‡yhjJputative periplasmic M16 family chaperone
Reads supporting (aligned to +/- strand):  ref base A (56/55);  new base G (4/4);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01

Only 100 of 125 total aligned reads displayed.

CTTTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGT  >  NC_000913/3680983‑3681281
                                                                                                                                                     |                                                                                                                                                     
cTTTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAACAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCa                                                                                                                                                       <  1:1176116/150‑1 (MQ=255)
  ttGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCGCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCAcc                                                                                                                                                     <  2:338968/150‑1 (MQ=255)
    gCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACcgc                                                                                                                                                   <  1:959350/150‑1 (MQ=255)
    gCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACcgc                                                                                                                                                   >  2:1043811/1‑150 (MQ=255)
       gTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGCATCGGCTGCCACGGCGGATCCCACAGGATAGATAACCGGTCCTGACGCACcgcggc                                                                                                                                                >  1:80368/1‑148 (MQ=255)
         tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCTCACGCCAGTAGAGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGAGTATCCCACATGATAGATAACCGGTCCTGACGCACAGCgtcat                                                                                                                                              >  1:1038014/1‑149 (MQ=255)
          aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCgtcagg                                                                                                                                             >  2:799439/1‑149 (MQ=255)
          aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCgtcagt                                                                                                                                             <  2:107391/150‑1 (MQ=255)
           cTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcagga                                                                                                                                            <  1:953813/150‑3 (MQ=255)
            tGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcaggac                                                                                                                                           <  1:1684653/150‑4 (MQ=255)
               cACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcaggaccgg                                                                                                                                        <  2:1357660/150‑7 (MQ=255)
                aCTTAACGCTTGCTTAACATGCCATAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                       <  2:807558/150‑1 (MQ=255)
                 cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataata                                                                                                                                      >  2:1452651/1‑150 (MQ=255)
                 cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataata                                                                                                                                      >  2:1248597/1‑150 (MQ=255)
                      cGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCAc                                                                                                                                 >  2:1207832/1‑150 (MQ=255)
                       gCTTGCTGAACATGCCAGAACTGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAAGCGGCTGCCACGGCGTATCCCACATGATAGATATCCGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                                                <  2:890580/150‑1 (MQ=255)
                       gCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                                                <  2:1443435/150‑1 (MQ=255)
                          tGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCt                                                                                                                             >  1:1485680/1‑150 (MQ=255)
                           gCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCtt                                                                                                                            >  2:122795/1‑150 (MQ=255)
                            cTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTc                                                                                                                           >  2:1463042/1‑150 (MQ=255)
                              gAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcg                                                                                                                         >  2:786284/1‑150 (MQ=255)
                                  aTGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacg                                                                                                                     >  1:464907/1‑150 (MQ=255)
                                   tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCAGATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacgc                                                                                                                    >  2:61517/1‑150 (MQ=255)
                                   tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                       >  2:1330013/1‑131 (MQ=255)
                                   tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                       <  1:1330013/131‑1 (MQ=255)
                                         aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCTGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGga                                                                                                              <  1:1665192/150‑1 (MQ=255)
                                          aCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGgag                                                                                                             <  2:1488012/150‑1 (MQ=255)
                                             gcgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                          >  2:256803/1‑150 (MQ=255)
                                             ctggcgtgcgGACCTGGCCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                          >  2:412389/8‑150 (MQ=255)
                                              cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                                         >  2:1117840/1‑150 (MQ=255)
                                              cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                                         <  1:1283642/150‑1 (MQ=255)
                                               gcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCGCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGc                                                                                                        <  2:1506664/150‑1 (MQ=255)
                                                 cTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGt                                                                                                      >  1:575602/1‑150 (MQ=255)
                                                            ggTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAg                                                                                           >  2:644408/1‑150 (MQ=255)
                                                            ggTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAg                                                                                           >  1:1154178/1‑150 (MQ=255)
                                                             gTCCGCACGCCAGTAGCGAAGCAGTTCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGc                                                                                          <  1:1513126/150‑1 (MQ=255)
                                                               ccGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTg                                                                                        >  2:238861/1‑150 (MQ=255)
                                                                cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                       <  2:1401819/150‑1 (MQ=255)
                                                                cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                       <  2:1218721/150‑1 (MQ=255)
                                                                cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                       <  2:464907/150‑1 (MQ=255)
                                                                 gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  2:555618/150‑1 (MQ=255)
                                                                 gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  2:1529148/150‑1 (MQ=255)
                                                                 gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  1:122795/150‑1 (MQ=255)
                                                                 gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  2:1535700/150‑1 (MQ=255)
                                                                  cACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCg                                                                                     >  1:1618749/1‑150 (MQ=255)
                                                                       cAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCa                                                                                <  1:1562946/150‑1 (MQ=255)
                                                                         gTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACg                                                                              <  2:1410974/150‑1 (MQ=255)
                                                                           aGCGCAGCAGTGAGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACAAGAAAGATAAAATATCCTGACGAACCGAGTCAGTAATAATACTCACCTCTTCCCAACGAAGCGGAGCAAGCGTAGGACCCGGAGCTGGCGTTTCACAtt                                                                            >  2:1166985/1‑150 (MQ=255)
                                                                             cgcAGCAGTGCGGCTGATTCACGTATCGGCTGCCACGGCGTATCCCACATGATAGATATCCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                          <  2:66395/150‑1 (MQ=255)
                                                                             cgcAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                          <  2:80368/150‑1 (MQ=255)
                                                                              gcagcaGTGCGGCTGATTCACTAATCGGCTGCCACGGCGTATCCCACATTATAGAAAACCGGTCCTGCCGCCCCGCGTCAGTCATAATACTCACCGCTTCCGCACTCAGCGGAGAAAGCGACGCCACCGGAGCTGGCGTTTAACTTTAGc                                                                         >  2:1053271/1‑150 (MQ=255)
                                                                                   agTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTc                                                                    <  2:1617505/150‑1 (MQ=255)
                                                                                    gTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCa                                                                   <  1:492294/150‑1 (MQ=255)
                                                                                     tGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCAGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAg                                                                  <  2:552440/150‑1 (MQ=255)
                                                                                         gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:432848/150‑1 (MQ=255)
                                                                                         gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:722508/150‑1 (MQ=255)
                                                                                         gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:60481/150‑1 (MQ=255)
                                                                                           tGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGcc                                                            <  1:419198/150‑1 (MQ=255)
                                                                                            gATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCa                                                           <  2:512669/150‑1 (MQ=255)
                                                                                              ttCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCaaa                                                         >  1:473338/1‑150 (MQ=255)
                                                                                                 aCGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGt                                                      >  1:1388333/1‑150 (MQ=255)
                                                                                                  cGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCCGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTAGCCAAACGtt                                                     >  1:196238/1‑150 (MQ=255)
                                                                                                   gAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGttt                                                    <  1:40492/150‑1 (MQ=255)
                                                                                                    aaTCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtttt                                                   >  2:435937/1‑150 (MQ=255)
                                                                                                    aaTCGGCTGCCACGGCGTATCCCACATGAAAGATAACCGGTCCTGACGCACCGCGTAAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtttt                                                   <  2:607759/150‑1 (MQ=255)
                                                                                                     aTCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTg                                                  >  2:806237/1‑150 (MQ=255)
                                                                                                        ggCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGttt                                               >  1:390038/1‑150 (MQ=255)
                                                                                                        ggCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGttt                                               >  1:137257/1‑150 (MQ=255)
                                                                                                         gCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTa                                              <  2:1423510/150‑1 (MQ=255)
                                                                                                           tGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                                            <  1:31744/150‑1 (MQ=255)
                                                                                                           tGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                                            >  2:113512/1‑150 (MQ=255)
                                                                                                            gCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAttt                                           >  1:472899/1‑150 (MQ=255)
                                                                                                             ccACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCTGAGAAACCGACGGCACCGGAGCTGGCGTTTCACGTTTTCCTTTCAGTTCGCCAAACGTTTTGTTTATTTg                                          >  1:1092449/1‑150 (MQ=255)
                                                                                                               aCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGt                                        >  2:98575/1‑150 (MQ=255)
                                                                                                                cGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGGCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTc                                       <  1:644408/150‑1 (MQ=255)
                                                                                                                 ggCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCg                                      <  2:907625/150‑1 (MQ=255)
                                                                                                                 ggCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGGCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCg                                      <  1:1481711/150‑1 (MQ=255)
                                                                                                                  gCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGa                                     >  1:1413357/1‑150 (MQ=255)
                                                                                                                    gTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACa                                   >  1:1655837/1‑150 (MQ=255)
                                                                                                                        cccACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCg                               <  1:1117840/150‑1 (MQ=255)
                                                                                                                        cccACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCg                               <  1:256803/150‑1 (MQ=255)
                                                                                                                               gatagataACCGGGCCTGTCGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAgcgcgc                        <  2:1241290/150‑1 (MQ=255)
                                                                                                                                atagataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCa                       <  1:1043811/150‑1 (MQ=255)
                                                                                                                                atagataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCa                       >  1:942132/1‑150 (MQ=255)
                                                                                                                                 tagatcACCGGTCCTGCCGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCAt                      <  1:781318/150‑1 (MQ=255)
                                                                                                                                   gataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTATATTTGGACGACAACCGAGCGCGCAtaa                    >  1:27746/1‑148 (MQ=255)
                                                                                                                                     taACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCAc                  <  2:1413357/150‑1 (MQ=255)
                                                                                                                                        ccGGTCCTGACGCCCCGCGGCAGTCATAATACTCCCCGCTTCCGCACGCAGCGGAGACAGCGGCGGCACCGGAGCTGGCGTTTCCCGTGTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACACCCGAGCGCGCATCCACGtt               <  2:76075/150‑1 (MQ=255)
                                                                                                                                           gTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTcc            <  2:1388333/150‑1 (MQ=255)
                                                                                                                                            gacTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTccc           >  2:975392/3‑150 (MQ=255)
                                                                                                                                             ccTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCcca          >  1:635003/1‑150 (MQ=255)
                                                                                                                                             acTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTAGCCAAACGTTTTGTTTATTTGGTCGACAACAGCGCTCGCATCCACTTTTcaca          >  2:314489/2‑150 (MQ=255)
                                                                                                                                              cTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGGCGGCACCGGAGCTGGCGTTTCACGTGTGCCTTTCAGTTCGCCAAACGGTTTGTTTATTTGGTCGACAACCGAGCGCGCCTCCACGTTTCccac         >  2:686016/1‑150 (MQ=255)
                                                                                                                                               tGACGCACCGCGGCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccacc        <  1:238861/150‑1 (MQ=255)
                                                                                                                                                gACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccacca       >  2:682103/1‑150 (MQ=255)
                                                                                                                                                  cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc     <  1:682103/150‑1 (MQ=255)
                                                                                                                                                  cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc     <  2:575602/150‑1 (MQ=255)
                                                                                                                                                    cACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAg   >  1:1556102/1‑150 (MQ=255)
                                                                                                                                                     aCCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGt  >  1:1432272/1‑150 (MQ=255)
                                                                                                                                                     aCCGCGTCAGTCATAATACTCACCGCTTCAGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGt  >  1:1600613/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGT  >  NC_000913/3680983‑3681281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: