Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,681,133 C→G 7.2% V270L (GTG→CTG) ‡ yhjJ ← putative periplasmic M16 family chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,681,1330CG 7.2% 413.0 / 15.7 122V270L (GTG→CTG) ‡yhjJputative periplasmic M16 family chaperone
Reads supporting (aligned to +/- strand):  ref base C (59/54);  new base G (4/5);  total (63/59)
Fisher's exact test for biased strand distribution p-value = 7.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 124 total aligned reads displayed.

TTTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGT  >  NC_000913/3680984‑3681281
                                                                                                                                                     |                                                                                                                                                    
tttGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCAc                                                                                                                                                      >  2:1562946/1‑150 (MQ=255)
 ttGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCGCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCAcc                                                                                                                                                     <  2:338968/150‑1 (MQ=255)
   gCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACcgc                                                                                                                                                   >  2:1043811/1‑150 (MQ=255)
      gTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGCATCGGCTGCCACGGCGGATCCCACAGGATAGATAACCGGTCCTGACGCACcgcggc                                                                                                                                                >  1:80368/1‑148 (MQ=255)
        tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCTCACGCCAGTAGAGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGAGTATCCCACATGATAGATAACCGGTCCTGACGCACAGCgtcat                                                                                                                                              >  1:1038014/1‑149 (MQ=255)
         aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCgtcagg                                                                                                                                             >  2:799439/1‑149 (MQ=255)
         aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcagg                                                                                                                                             <  1:1516071/150‑2 (MQ=255)
         aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCgtcagt                                                                                                                                             <  2:107391/150‑1 (MQ=255)
          cTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcagga                                                                                                                                            <  1:953813/150‑3 (MQ=255)
           tGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcaggac                                                                                                                                           <  1:1684653/150‑4 (MQ=255)
               aCTTAACGCTTGCTTAACATGCCATAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                       <  2:807558/150‑1 (MQ=255)
                cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataata                                                                                                                                      >  2:1452651/1‑150 (MQ=255)
                cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataata                                                                                                                                      >  2:1248597/1‑150 (MQ=255)
                     cGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCAc                                                                                                                                 >  2:1207832/1‑150 (MQ=255)
                      gCTTGCTGAACATGCCAGAACTGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAAGCGGCTGCCACGGCGTATCCCACATGATAGATATCCGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                                                <  2:890580/150‑1 (MQ=255)
                      gCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                                                <  2:1443435/150‑1 (MQ=255)
                         tGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCt                                                                                                                             >  1:1485680/1‑150 (MQ=255)
                            tGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTcc                                                                                                                          <  1:1324582/150‑1 (MQ=255)
                            tGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTcc                                                                                                                          >  2:1481711/1‑150 (MQ=255)
                             gAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcg                                                                                                                         >  2:786284/1‑150 (MQ=255)
                                 aTGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacg                                                                                                                     >  1:464907/1‑150 (MQ=255)
                                  tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCAGATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacgc                                                                                                                    >  2:61517/1‑150 (MQ=255)
                                  tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                       >  2:1330013/1‑131 (MQ=255)
                                  tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                       <  1:1330013/131‑1 (MQ=255)
                                        aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGga                                                                                                              <  1:67637/150‑1 (MQ=255)
                                         aCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCAGCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGgag                                                                                                             <  2:14180/150‑1 (MQ=255)
                                         aCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGgag                                                                                                             <  2:1488012/150‑1 (MQ=255)
                                          cAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCAGAATACTCACCGCTTCCGCACGCAGCGgcga                                                                                                            >  1:163334/1‑150 (MQ=255)
                                            gcgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                          >  2:256803/1‑150 (MQ=255)
                                            gcgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                          >  2:1363827/1‑150 (MQ=255)
                                            ctggcgtgcgGACCTGGCCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                          >  2:412389/8‑150 (MQ=255)
                                             cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                                         <  1:1283642/150‑1 (MQ=255)
                                              gcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCGCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGc                                                                                                        <  2:1506664/150‑1 (MQ=255)
                                                cTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGt                                                                                                      >  1:575602/1‑150 (MQ=255)
                                                cTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCATATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGt                                                                                                      >  1:1415255/1‑150 (MQ=255)
                                                           ggTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAg                                                                                           >  2:644408/1‑150 (MQ=255)
                                                            gTCCGCACGCCAGTAGCGAAGCAGTTCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGc                                                                                          <  1:1513126/150‑1 (MQ=255)
                                                              ccGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTg                                                                                        >  2:238861/1‑150 (MQ=255)
                                                               cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                       <  2:1401819/150‑1 (MQ=255)
                                                               cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                       <  2:1218721/150‑1 (MQ=255)
                                                               cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                       <  2:464907/150‑1 (MQ=255)
                                                                gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  2:555618/150‑1 (MQ=255)
                                                                gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  2:1529148/150‑1 (MQ=255)
                                                                gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                      <  1:122795/150‑1 (MQ=255)
                                                                 cACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCg                                                                                     >  1:1618749/1‑150 (MQ=255)
                                                                        gTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACg                                                                              <  2:1410974/150‑1 (MQ=255)
                                                                          aGCGCAGCAGTGAGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACAAGAAAGATAAAATATCCTGACGAACCGAGTCAGTAATAATACTCACCTCTTCCCAACGAAGCGGAGCAAGCGTAGGACCCGGAGCTGGCGTTTCACAtt                                                                            >  2:1166985/1‑150 (MQ=255)
                                                                            cgcAGCAGTGCGGCTGATTCACGTATCGGCTGCCACGGCGTATCCCACATGATAGATATCCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                          <  2:66395/150‑1 (MQ=255)
                                                                            cgcAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                          <  2:80368/150‑1 (MQ=255)
                                                                            cgcAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                          <  1:458165/150‑1 (MQ=255)
                                                                             gcagcaGTGCGGCTGATTCACTAATCGGCTGCCACGGCGTATCCCACATTATAGAAAACCGGTCCTGCCGCCCCGCGTCAGTCATAATACTCACCGCTTCCGCACTCAGCGGAGAAAGCGACGCCACCGGAGCTGGCGTTTAACTTTAGc                                                                         >  2:1053271/1‑150 (MQ=255)
                                                                                  agTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTc                                                                    <  2:1617505/150‑1 (MQ=255)
                                                                                   gTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCa                                                                   <  1:492294/150‑1 (MQ=255)
                                                                                    tGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCAGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAg                                                                  <  2:552440/150‑1 (MQ=255)
                                                                                        gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:722508/150‑1 (MQ=255)
                                                                                        gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:1150377/150‑1 (MQ=255)
                                                                                        gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:60481/150‑1 (MQ=255)
                                                                                        gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                              <  1:432848/150‑1 (MQ=255)
                                                                                           gATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCa                                                           <  2:512669/150‑1 (MQ=255)
                                                                                                aCGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGt                                                      >  1:1388333/1‑150 (MQ=255)
                                                                                                 cGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCCGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTAGCCAAACGtt                                                     >  1:196238/1‑150 (MQ=255)
                                                                                                  gAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGttt                                                    <  1:40492/150‑1 (MQ=255)
                                                                                                   aaTCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtttt                                                   >  2:435937/1‑150 (MQ=255)
                                                                                                   aaTCGGCTGCCACGGCGTATCCCACATGAAAGATAACCGGTCCTGACGCACCGCGTAAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtttt                                                   <  2:607759/150‑1 (MQ=255)
                                                                                                    aTCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTg                                                  >  2:806237/1‑150 (MQ=255)
                                                                                                     tCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGt                                                 >  2:31744/1‑150 (MQ=255)
                                                                                                       ggCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGttt                                               >  1:390038/1‑150 (MQ=255)
                                                                                                        gCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTa                                              <  2:1423510/150‑1 (MQ=255)
                                                                                                          tGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                                            >  2:113512/1‑150 (MQ=255)
                                                                                                          tGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                                            <  1:31744/150‑1 (MQ=255)
                                                                                                           gCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAttt                                           >  1:472899/1‑150 (MQ=255)
                                                                                                            ccACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCTGAGAAACCGACGGCACCGGAGCTGGCGTTTCACGTTTTCCTTTCAGTTCGCCAAACGTTTTGTTTATTTg                                          >  1:1092449/1‑150 (MQ=255)
                                                                                                              aCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGt                                        >  2:98575/1‑150 (MQ=255)
                                                                                                               cGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGGCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTc                                       <  1:644408/150‑1 (MQ=255)
                                                                                                                ggCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCg                                      <  2:907625/150‑1 (MQ=255)
                                                                                                                 gCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGa                                     >  1:1413357/1‑150 (MQ=255)
                                                                                                                   gTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACa                                   <  2:1154178/150‑1 (MQ=255)
                                                                                                                   gTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACa                                   >  1:1655837/1‑150 (MQ=255)
                                                                                                                       cccACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCg                               <  1:1117840/150‑1 (MQ=255)
                                                                                                                           caTGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAgcg                           >  2:1230893/1‑150 (MQ=255)
                                                                                                                             tGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCTCCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCCCCGGAGCTGGCGTTTCACGTATGCCTTTCACTTCGCCAAACGTTATGGTTATTTGGACGACAACCGAgcgcg                         >  1:219561/1‑150 (MQ=255)
                                                                                                                              gatagataACCGGGCCTGTCGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAgcgcgc                        <  2:1241290/150‑1 (MQ=255)
                                                                                                                               atagataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCa                       <  1:1043811/150‑1 (MQ=255)
                                                                                                                               atagataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCa                       >  1:942132/1‑150 (MQ=255)
                                                                                                                                  gataACCGGTCCTGACGCTCCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATcc                    >  1:33383/1‑150 (MQ=255)
                                                                                                                                  gataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTATATTTGGACGACAACCGAGCGCGCAtaa                    >  1:27746/1‑148 (MQ=255)
                                                                                                                                       ccGGTCCTGACGCCCCGCGGCAGTCATAATACTCCCCGCTTCCGCACGCAGCGGAGACAGCGGCGGCACCGGAGCTGGCGTTTCCCGTGTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACACCCGAGCGCGCATCCACGtt               <  2:76075/150‑1 (MQ=255)
                                                                                                                                          tgacTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCCCCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAGTTGGTCGACAACCGAGCGCGCATCCACGTTTcc            >  2:1052109/4‑150 (MQ=255)
                                                                                                                                          gTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTcc            <  2:1388333/150‑1 (MQ=255)
                                                                                                                                           gacTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTccc           >  2:975392/3‑150 (MQ=255)
                                                                                                                                            acTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCcca          >  1:870628/2‑150 (MQ=255)
                                                                                                                                             cTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGGCGGCACCGGAGCTGGCGTTTCACGTGTGCCTTTCAGTTCGCCAAACGGTTTGTTTATTTGGTCGACAACCGAGCGCGCCTCCACGTTTCccac         >  2:686016/1‑150 (MQ=255)
                                                                                                                                              tGACGCACCGCGGCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccacc        <  1:238861/150‑1 (MQ=255)
                                                                                                                                               gACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccacca       >  2:682103/1‑150 (MQ=255)
                                                                                                                                                 cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc     <  1:682103/150‑1 (MQ=255)
                                                                                                                                                 cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc     >  1:534975/1‑150 (MQ=255)
                                                                                                                                                 cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc     <  2:575602/150‑1 (MQ=255)
                                                                                                                                                   cACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAg   >  1:1556102/1‑150 (MQ=255)
                                                                                                                                                    aCCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGt  >  1:1432272/1‑150 (MQ=255)
                                                                                                                                                    aCCGCGTCAGTCATAATACTCACCGCTTCAGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGt  >  1:1600613/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGT  >  NC_000913/3680984‑3681281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: