Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,681,134 C→T 6.7% A269A (GCG→GCA yhjJ ← putative periplasmic M16 family chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,681,1340CT 6.7% 371.5 / 8.1 119A269A (GCG→GCAyhjJputative periplasmic M16 family chaperone
Reads supporting (aligned to +/- strand):  ref base C (56/54);  new base T (4/4);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAG  >  NC_000913/3680985‑3681283
                                                                                                                                                     |                                                                                                                                                     
ttGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCGCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCAcc                                                                                                                                                       <  2:338968/150‑1 (MQ=255)
  gCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACcgc                                                                                                                                                     <  1:959350/150‑1 (MQ=255)
  gCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACcgc                                                                                                                                                     >  2:1043811/1‑150 (MQ=255)
     gTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGCATCGGCTGCCACGGCGGATCCCACAGGATAGATAACCGGTCCTGACGCACcgcggc                                                                                                                                                  >  1:80368/1‑148 (MQ=255)
       tACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCTCACGCCAGTAGAGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGAGTATCCCACATGATAGATAACCGGTCCTGACGCACAGCgtcat                                                                                                                                                >  1:1038014/1‑149 (MQ=255)
        aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCgtcagg                                                                                                                                               >  2:799439/1‑149 (MQ=255)
        aCTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcagg                                                                                                                                               <  1:1516071/150‑2 (MQ=255)
          tGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcaggac                                                                                                                                             <  1:1684653/150‑4 (MQ=255)
             cACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCGGTGCgtcaggaccgg                                                                                                                                          <  2:1357660/150‑7 (MQ=255)
              aCTTAACGCTTGCTTAACATGCCATAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataat                                                                                                                                         <  2:807558/150‑1 (MQ=255)
               cTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCataata                                                                                                                                        >  2:1248597/1‑150 (MQ=255)
                    cGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCAc                                                                                                                                   >  2:1207832/1‑150 (MQ=255)
                     gCTTGCTGAACATGCCAGAACTGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAAGCGGCTGCCACGGCGTATCCCACATGATAGATATCCGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                                                  <  2:890580/150‑1 (MQ=255)
                     gCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCAcc                                                                                                                                  <  2:1443435/150‑1 (MQ=255)
                        tGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCt                                                                                                                               >  1:1485680/1‑150 (MQ=255)
                         gCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCtt                                                                                                                              >  2:122795/1‑150 (MQ=255)
                          cTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTc                                                                                                                             >  2:1463042/1‑150 (MQ=255)
                           tGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTcc                                                                                                                            <  1:1324582/150‑1 (MQ=255)
                           tGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTcc                                                                                                                            >  2:1481711/1‑150 (MQ=255)
                            gAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcg                                                                                                                           >  2:786284/1‑150 (MQ=255)
                                aTGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacg                                                                                                                       >  1:464907/1‑150 (MQ=255)
                                 tGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCAGATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCcgcacgc                                                                                                                      >  2:61517/1‑150 (MQ=255)
                                       aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCTGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGga                                                                                                                <  1:1665192/150‑1 (MQ=255)
                                       aaCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGga                                                                                                                <  1:67637/150‑1 (MQ=255)
                                        aCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCAGCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGgag                                                                                                               <  2:14180/150‑1 (MQ=255)
                                        aCAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGgag                                                                                                               <  2:1488012/150‑1 (MQ=255)
                                         cAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCAGAATACTCACCGCTTCCGCACGCAGCGgcga                                                                                                              >  1:163334/1‑150 (MQ=255)
                                           gcgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                            >  2:256803/1‑150 (MQ=255)
                                           gcgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                            >  2:1363827/1‑150 (MQ=255)
                                           ctggcgtgcgGACCTGGCCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGaaa                                                                                                            >  2:412389/8‑150 (MQ=255)
                                            cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                                           >  2:1117840/1‑150 (MQ=255)
                                            cgcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAg                                                                                                           <  1:1283642/150‑1 (MQ=255)
                                             gcCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCGCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGc                                                                                                          <  2:1506664/150‑1 (MQ=255)
                                               cTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGt                                                                                                        >  1:575602/1‑150 (MQ=255)
                                               cTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCATATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGt                                                                                                        >  1:1415255/1‑150 (MQ=255)
                                                          ggTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAg                                                                                             >  2:644408/1‑150 (MQ=255)
                                                           gTCCGCACGCCAGTAGCGAAGCAGTTCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGc                                                                                            <  1:1513126/150‑1 (MQ=255)
                                                             ccGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTg                                                                                          >  2:238861/1‑150 (MQ=255)
                                                              cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                         <  2:1401819/150‑1 (MQ=255)
                                                              cGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTgg                                                                                         <  2:464907/150‑1 (MQ=255)
                                                               gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                        <  2:1535700/150‑1 (MQ=255)
                                                               gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                        <  2:555618/150‑1 (MQ=255)
                                                               gCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGc                                                                                        <  1:122795/150‑1 (MQ=255)
                                                                cACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCg                                                                                       >  1:1618749/1‑150 (MQ=255)
                                                                     cAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCa                                                                                  <  1:1562946/150‑1 (MQ=255)
                                                                         aGCGCAGCAGTGAGGCTGATTCACGAATCGGCTGCCCCGGCGTATCCCACAAGAAAGATAAAATATCCTGACGAACCGAGTCAGTAATAATACTCACCTCTTCCCAACGAAGCGGAGCAAGCGTAGGACCCGGAGCTGGCGTTTCACAtt                                                                              >  2:1166985/1‑150 (MQ=255)
                                                                           cgcAGCAGTGCGGCTGATTCACGTATCGGCTGCCACGGCGTATCCCACATGATAGATATCCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                            <  2:66395/150‑1 (MQ=255)
                                                                           cgcAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                            <  1:458165/150‑1 (MQ=255)
                                                                           cgcAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTg                                                                            <  2:80368/150‑1 (MQ=255)
                                                                            gcagcaGTGCGGCTGATTCACTAATCGGCTGCCACGGCGTATCCCACATTATAGAAAACCGGTCCTGCCGCCCCGCGTCAGTCATAATACTCACCGCTTCCGCACTCAGCGGAGAAAGCGACGCCACCGGAGCTGGCGTTTAACTTTAGc                                                                           >  2:1053271/1‑150 (MQ=255)
                                                                                 agTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTc                                                                      <  2:1617505/150‑1 (MQ=255)
                                                                                  gTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCa                                                                     <  1:492294/150‑1 (MQ=255)
                                                                                   tGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCAGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAg                                                                    <  2:552440/150‑1 (MQ=255)
                                                                                       gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                                <  1:1150377/150‑1 (MQ=255)
                                                                                       gCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCg                                                                <  1:432848/150‑1 (MQ=255)
                                                                                         tGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGcc                                                              <  1:419198/150‑1 (MQ=255)
                                                                                          gATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCa                                                             <  2:512669/150‑1 (MQ=255)
                                                                                            ttCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCaaa                                                           >  1:473338/1‑150 (MQ=255)
                                                                                               aCGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGt                                                        >  1:1388333/1‑150 (MQ=255)
                                                                                                cGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCCGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTAGCCAAACGtt                                                       >  1:196238/1‑150 (MQ=255)
                                                                                                  aaTCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtttt                                                     >  2:435937/1‑150 (MQ=255)
                                                                                                  aaTCGGCTGCCACGGCGTATCCCACATGAAAGATAACCGGTCCTGACGCACCGCGTAAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGtttt                                                     <  2:607759/150‑1 (MQ=255)
                                                                                                   aTCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTg                                                    >  2:806237/1‑150 (MQ=255)
                                                                                                    tCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGt                                                   >  2:31744/1‑150 (MQ=255)
                                                                                                      ggCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGttt                                                 >  1:137257/1‑150 (MQ=255)
                                                                                                      ggCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGttt                                                 >  1:390038/1‑150 (MQ=255)
                                                                                                       gCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTa                                                <  2:1423510/150‑1 (MQ=255)
                                                                                                         tGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                                              >  2:113512/1‑150 (MQ=255)
                                                                                                         tGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAtt                                              <  1:31744/150‑1 (MQ=255)
                                                                                                           ccACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCTGAGAAACCGACGGCACCGGAGCTGGCGTTTCACGTTTTCCTTTCAGTTCGCCAAACGTTTTGTTTATTTg                                            >  1:1092449/1‑150 (MQ=255)
                                                                                                             aCGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGt                                          >  2:98575/1‑150 (MQ=255)
                                                                                                              cGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGGCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTc                                         <  1:644408/150‑1 (MQ=255)
                                                                                                               ggCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCg                                        <  2:907625/150‑1 (MQ=255)
                                                                                                               ggCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGGCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCg                                        <  1:1481711/150‑1 (MQ=255)
                                                                                                                gCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGa                                       >  1:1413357/1‑150 (MQ=255)
                                                                                                                  gTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACa                                     <  2:1154178/150‑1 (MQ=255)
                                                                                                                      cccACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCg                                 <  1:1117840/150‑1 (MQ=255)
                                                                                                                      cccACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCg                                 <  1:256803/150‑1 (MQ=255)
                                                                                                                          caTGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAgcg                             >  2:1230893/1‑150 (MQ=255)
                                                                                                                            tGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCTCCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCCCCGGAGCTGGCGTTTCACGTATGCCTTTCACTTCGCCAAACGTTATGGTTATTTGGACGACAACCGAgcgcg                           >  1:219561/1‑150 (MQ=255)
                                                                                                                             gatagataACCGGGCCTGTCGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAgcgcgc                          <  2:1241290/150‑1 (MQ=255)
                                                                                                                              atagataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCa                         <  1:1043811/150‑1 (MQ=255)
                                                                                                                              atagataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCa                         >  1:942132/1‑150 (MQ=255)
                                                                                                                               tagatcACCGGTCCTGCCGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCAt                        <  1:781318/150‑1 (MQ=255)
                                                                                                                                 gataACCGGTCCTGACGCTCCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATcc                      >  1:33383/1‑150 (MQ=255)
                                                                                                                                 gataACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTATATTTGGACGACAACCGAGCGCGCAtaa                      >  1:27746/1‑148 (MQ=255)
                                                                                                                                   taACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCAc                    <  2:1413357/150‑1 (MQ=255)
                                                                                                                                      ccGGTCCTGACGCCCCGCGGCAGTCATAATACTCCCCGCTTCCGCACGCAGCGGAGACAGCGGCGGCACCGGAGCTGGCGTTTCCCGTGTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACACCCGAGCGCGCATCCACGtt                 <  2:76075/150‑1 (MQ=255)
                                                                                                                                         tgacTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCCCCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTAGTTGGTCGACAACCGAGCGCGCATCCACGTTTcc              >  2:1052109/4‑150 (MQ=255)
                                                                                                                                         gTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTcc              <  2:1388333/150‑1 (MQ=255)
                                                                                                                                          gacTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTccc             >  2:975392/3‑150 (MQ=255)
                                                                                                                                           ccTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCcca            >  1:635003/1‑150 (MQ=255)
                                                                                                                                           acTGACGCGGTGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCcca            >  1:870628/2‑150 (MQ=255)
                                                                                                                                            cTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGGCGGCACCGGAGCTGGCGTTTCACGTGTGCCTTTCAGTTCGCCAAACGGTTTGTTTATTTGGTCGACAACCGAGCGCGCCTCCACGTTTCccac           >  2:686016/1‑150 (MQ=255)
                                                                                                                                              gACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccacca         >  2:682103/1‑150 (MQ=255)
                                                                                                                                                cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc       >  1:534975/1‑150 (MQ=255)
                                                                                                                                                cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc       <  2:575602/150‑1 (MQ=255)
                                                                                                                                                cGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCccaccacc       <  1:682103/150‑1 (MQ=255)
                                                                                                                                                   aCCGCGTCAGTCATAATACTCACCGCTTCAGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGt    >  1:1600613/1‑150 (MQ=255)
                                                                                                                                                     cgcgTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAg  <  1:1207832/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGCTGTTACTGGCACTTAACGCTTGCTGAACATGCCAGAACAGCGCCTCGCGGGCCAGGTCCGCACGCCAGTAGCGCAGCAGTGCGGCTGATTCACGAATCGGCTGCCACGGCGTATCCCACATGATAGATAACCGGTCCTGACGCACCGCGTCAGTCATAATACTCACCGCTTCCGCACGCAGCGGAGAAAGCGTCGGCACCGGAGCTGGCGTTTCACGTTTGCCTTTCAGTTCGCCAAACGTTTTGTTTATTTGGTCGACAACCGAGCGCGCATCCACGTTTCCCACCACCAGTAG  >  NC_000913/3680985‑3681283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: