Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,700,416 T→C 5.0% intergenic (+215/‑147) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,700,4160TC 5.0% 422.4 / 9.0 139intergenic (+215/‑147)rdlD/yhjVsRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
Reads supporting (aligned to +/- strand):  ref base T (66/66);  new base C (4/3);  total (70/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01

Only 100 of 143 total aligned reads displayed.

CGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATG  >  NC_000913/3700267‑3700565
                                                                                                                                                     |                                                                                                                                                     
 ggCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAAtttt                                                                                                                                                      <  2:1543252/150‑1 (MQ=255)
   cTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGt                                                                                                                                                    <  2:765588/150‑1 (MQ=255)
    ttGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATAGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTAATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTa                                                                                                                                                   <  2:1053561/150‑1 (MQ=255)
     tGCCGCTGATTCTACGATTGATTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAg                                                                                                                                                  <  2:273360/150‑1 (MQ=255)
      gCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGa                                                                                                                                                 >  1:857304/1‑150 (MQ=255)
            gATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTgcg                                                                                                                                           <  1:1481516/150‑1 (MQ=255)
               tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcct                                                                                                                                        <  1:929082/150‑1 (MQ=255)
               tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcat                                                                                                                                        <  2:537037/150‑3 (MQ=255)
                cTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcatt                                                                                                                                       <  1:154327/150‑4 (MQ=255)
                  aCGATTGCTTTTTACTGTCAGCACCCGGCAATCTTACTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATACGGGCTGACTCCAGACTGGTAGTGAAATTCGCAATTTTGTAGAATTGCGcctcct                                                                                                                                     >  2:1319179/1‑150 (MQ=255)
                   cGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCctcctc                                                                                                                                    >  1:586591/1‑150 (MQ=255)
                   cGATTGCTTTTTACGATCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTTGTAGTGAAATGCGCAATTTTGAAGAATTGCGCctcctc                                                                                                                                    >  2:106269/1‑150 (MQ=255)
                     aTTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTGCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTcgc                                                                                                                                  >  1:781421/1‑150 (MQ=255)
                        gCTTTTTACTGTCAGCACCCGGCCATCTTCCTCATTGCGCGATGCCTTCGAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGTGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGAAGAATTGCGCCTCCTCGCGAc                                                                                                                               <  2:270577/150‑1 (MQ=255)
                        gCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                               >  1:1390063/1‑150 (MQ=255)
                        gCTTTTTACTGTCAGCACCCGGCAATCTTACTCATTGCGAGATGCCTTCAAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATACGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                               >  2:1025706/1‑150 (MQ=255)
                         cTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAcc                                                                                                                              >  2:659450/1‑150 (MQ=255)
                         cTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAcc                                                                                                                              >  1:63996/1‑150 (MQ=255)
                         cTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAcc                                                                                                                              >  1:386713/1‑150 (MQ=255)
                           ttttACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTc                                                                                                                            <  2:47728/150‑1 (MQ=255)
                              tACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTcccc                                                                                                                         <  2:195536/150‑1 (MQ=255)
                                  gTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTg                                                                                                                     <  2:1442243/150‑1 (MQ=255)
                                  gTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTg                                                                                                                     >  1:23788/1‑150 (MQ=255)
                                  gTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTg                                                                                                                     >  1:998168/1‑150 (MQ=255)
                                   tCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGa                                                                                                                    <  2:1455159/150‑1 (MQ=255)
                                    cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                   >  2:1192216/1‑150 (MQ=255)
                                    cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                   >  1:1195912/1‑150 (MQ=255)
                                     aGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAAt                                                                                                                  >  2:963149/1‑150 (MQ=255)
                                      gCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATc                                                                                                                 <  1:334098/150‑1 (MQ=255)
                                      gCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATc                                                                                                                 <  2:586591/150‑1 (MQ=255)
                                      gCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATc                                                                                                                 <  1:659450/150‑1 (MQ=255)
                                          ccGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGtt                                                                                                             <  2:63996/150‑1 (MQ=255)
                                           cGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTg                                                                                                            >  1:1716648/1‑150 (MQ=255)
                                            ggCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGc                                                                                                           <  1:1473321/150‑1 (MQ=255)
                                            ggCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGc                                                                                                           >  1:1380135/1‑150 (MQ=255)
                                                  cTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAtt                                                                                                     >  2:1530576/1‑150 (MQ=255)
                                                     ccTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttttt                                                                                                  >  1:370959/1‑150 (MQ=255)
                                                     ccTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttttt                                                                                                  >  1:1247247/1‑150 (MQ=255)
                                                       tCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTa                                                                                                <  1:813763/150‑1 (MQ=255)
                                                        cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTTAATCTGTTGCCTCATTTTTTAc                                                                                               <  1:1026477/150‑1 (MQ=255)
                                                        cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGGGAATCTGTTGCCTCATTTTTTAc                                                                                               >  2:1094595/1‑150 (MQ=255)
                                                              gcgATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTg                                                                                         <  2:857304/150‑1 (MQ=255)
                                                                 aTGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGACTCATTTTTTTCATACTGAGt                                                                                      >  1:240480/1‑150 (MQ=255)
                                                                  tggcTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                     <  2:1603461/147‑1 (MQ=255)
                                                                   gCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGCATCTGTTGCCTCATTTTTTACATACTGCGTAt                                                                                    >  2:109182/1‑150 (MQ=255)
                                                                   gCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTAt                                                                                    >  1:690052/1‑150 (MQ=255)
                                                                      ttCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCg                                                                                 <  2:690052/150‑1 (MQ=255)
                                                                      ttCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGAATGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACATCACCAGTGAATCTGTTGCATCATTTTTTACATACTGCGTATTCg                                                                                 >  1:1047511/1‑150 (MQ=255)
                                                                        ccAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGAc                                                                               >  2:1140330/1‑150 (MQ=255)
                                                                          aGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGGCTCATATTTTACATACTGCGTATTCGACtt                                                                             >  1:181514/1‑150 (MQ=255)
                                                                          aGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTTCGTATTCGACtt                                                                             >  2:158539/1‑150 (MQ=255)
                                                                           gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtc                                                                            >  1:1601318/1‑150 (MQ=255)
                                                                           gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAGTTTTTACATACTGCGTATTCGACTtc                                                                            >  2:992604/1‑150 (MQ=255)
                                                                           gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGCTAGTGAAATGCGCAATTTTGTAGAATTGCGACTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtc                                                                            >  1:1245096/1‑150 (MQ=255)
                                                                              tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                         <  2:386792/150‑1 (MQ=255)
                                                                              tAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTcc                                                                         >  1:1621645/1‑150 (MQ=255)
                                                                               aaTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCTACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCa                                                                        <  1:1566108/150‑1 (MQ=255)
                                                                                   tCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCGTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTg                                                                    >  1:736886/1‑150 (MQ=255)
                                                                                     tttATTTGCAATAGTTGCAGTTCATATCCGGGCGGACTCCAGACTGGTAGTGAACTGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTCTACATACTGCGTATTCGACTTCTCCACCTGtt                                                                  <  1:1113409/150‑1 (MQ=255)
                                                                                      ttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTg                                                                 <  2:23788/150‑1 (MQ=255)
                                                                                        aTTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTgcg                                                               <  2:386713/150‑1 (MQ=255)
                                                                                        aTTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTACTCCACCTGTTgcg                                                               >  2:237756/1‑150 (MQ=255)
                                                                                            gCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAag                                                           <  2:1391262/150‑1 (MQ=255)
                                                                                               aTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAgaga                                                        <  1:111547/150‑1 (MQ=255)
                                                                                                    tGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTg                                                   >  1:378184/1‑150 (MQ=255)
                                                                                                     gCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTgg                                                  <  1:418442/150‑1 (MQ=255)
                                                                                                      cAGTACATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCTCAATTTTGTAGAATTGCGCCACCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATAATGCGTATTCGAATTCTCCAAATGTTGCCCAATAGATACTggg                                                 >  2:791868/1‑150 (MQ=255)
                                                                                                        gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                               <  2:877619/150‑1 (MQ=255)
                                                                                                          tCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTa                                             >  2:1158634/1‑150 (MQ=255)
                                                                                                          tCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTCCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTa                                             >  2:1709808/1‑150 (MQ=255)
                                                                                                           cATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTAt                                            >  2:595711/1‑150 (MQ=255)
                                                                                                            atatCCGGGCTGACTCCAGACTGGGAGTGAAATGCGCAATTTTTTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTAtt                                           <  1:106269/150‑1 (MQ=255)
                                                                                                              atCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCa                                         <  1:464872/150‑1 (MQ=255)
                                                                                                                ccGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAtt                                       <  1:1530576/150‑1 (MQ=255)
                                                                                                                      tGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCg                                 <  2:797431/150‑1 (MQ=255)
                                                                                                                        aCTCCAGTCTGGTAGTGAAATGCGCAATTTTGTAGAATTCCGCCTCCTCGCGACCTCCCCAGTGAATCTTTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAg                               <  2:111443/150‑1 (MQ=255)
                                                                                                                        aCTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAg                               <  2:74949/150‑1 (MQ=255)
                                                                                                                         cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAgg                              >  2:1664269/1‑150 (MQ=255)
                                                                                                                         cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAgg                              >  1:274809/1‑150 (MQ=255)
                                                                                                                         cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGAGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTGGCGCAAGAGAAAATGGGTTTATTCATTTTTGAGAgg                              >  1:1219352/1‑150 (MQ=255)
                                                                                                                          tCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGc                             <  2:1185774/150‑1 (MQ=255)
                                                                                                                           ccAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGcc                            <  1:1192216/150‑1 (MQ=255)
                                                                                                                            cAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCg                           >  1:87401/1‑150 (MQ=255)
                                                                                                                             aGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGa                          >  2:402756/1‑150 (MQ=255)
                                                                                                                                  ggTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtct                     >  1:936752/1‑150 (MQ=255)
                                                                                                                                   gTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtctt                    >  2:411347/1‑150 (MQ=255)
                                                                                                                                    tAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttcttt                   <  1:1025706/150‑1 (MQ=255)
                                                                                                                                     aGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttc                  <  2:1621645/150‑1 (MQ=255)
                                                                                                                                     aGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttc                  <  2:1245096/150‑1 (MQ=255)
                                                                                                                                          aaTGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGAc             <  2:1536947/150‑1 (MQ=255)
                                                                                                                                          aaTGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGAc             >  2:1622123/1‑150 (MQ=255)
                                                                                                                                            ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGCCTGCTTTCTGGACAg           >  1:1707925/2‑150 (MQ=255)
                                                                                                                                            ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg           >  1:1035092/2‑150 (MQ=255)
                                                                                                                                             gcgcAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGCAACTGGGTTTATTCATTTTTGAGAGGCCGACTTCTTTCTGGACAgg          >  2:569103/1‑150 (MQ=255)
                                                                                                                                              cgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATAATGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGa         >  2:590890/1‑150 (MQ=255)
                                                                                                                                                cAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACt       <  2:1469136/150‑1 (MQ=255)
                                                                                                                                                cAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACt       <  2:1379324/150‑1 (MQ=255)
                                                                                                                                                cAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACt       >  1:887804/1‑150 (MQ=255)
                                                                                                                                                   ttttGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTAATTTTTTACATACTGCGTATTCGCCTTCTCGACCTCTTGCGCAAGATAAACTGTGTTTATTCTTTTTTGAGAGGCAGACTTCTTTCGGTACAGGCCttga    >  2:311061/1‑148 (MQ=255)
                                                                                                                                                     ttGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATg  >  2:437365/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATG  >  NC_000913/3700267‑3700565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: