Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,700,419 T→C 5.0% intergenic (+218/‑144) rdlD → / → yhjV sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,700,4190TC 5.0% 424.1 / 9.0 142intergenic (+218/‑144)rdlD/yhjVsRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD/putative transporter
Reads supporting (aligned to +/- strand):  ref base T (68/65);  new base C (4/3);  total (73/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.19e-01

Only 100 of 145 total aligned reads displayed.

CTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATGCAG  >  NC_000913/3700270‑3700568
                                                                                                                                                     |                                                                                                                                                     
 ttGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATAGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTAATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTa                                                                                                                                                      <  2:1053561/150‑1 (MQ=255)
  tGCCGCTGATTCTACGATTGATTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAg                                                                                                                                                     <  2:273360/150‑1 (MQ=255)
         gATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTgcg                                                                                                                                              <  1:1481516/150‑1 (MQ=255)
            tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcct                                                                                                                                           <  1:929082/150‑1 (MQ=255)
            tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGcct                                                                                                                                           <  1:472443/150‑1 (MQ=255)
            tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcat                                                                                                                                           <  2:537037/150‑3 (MQ=255)
            tCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcat                                                                                                                                           <  1:1654160/150‑3 (MQ=255)
             cTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTCTACAAAATTgcgcatt                                                                                                                                          <  1:154327/150‑4 (MQ=255)
                cGATTGCTTTTTACGATCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTTGTAGTGAAATGCGCAATTTTGAAGAATTGCGCctcctc                                                                                                                                       >  2:106269/1‑150 (MQ=255)
                  aTTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTGCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTcgc                                                                                                                                     >  1:781421/1‑150 (MQ=255)
                     gCTTTTTACTGTCAGCACCCGGCCATCTTCCTCATTGCGCGATGCCTTCGAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGTGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGAAGAATTGCGCCTCCTCGCGAc                                                                                                                                  <  2:270577/150‑1 (MQ=255)
                     gCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                                  >  1:1390063/1‑150 (MQ=255)
                     gCTTTTTACTGTCAGCACCCGGCAATCTTACTCATTGCGAGATGCCTTCAAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATACGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAc                                                                                                                                  >  2:1025706/1‑150 (MQ=255)
                      cTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAcc                                                                                                                                 >  1:386713/1‑150 (MQ=255)
                      cTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAcc                                                                                                                                 >  1:63996/1‑150 (MQ=255)
                      cTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGAcc                                                                                                                                 >  2:659450/1‑150 (MQ=255)
                        ttttACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTc                                                                                                                               <  2:47728/150‑1 (MQ=255)
                               gTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTg                                                                                                                        >  1:998168/1‑150 (MQ=255)
                                tCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGa                                                                                                                       <  2:1455159/150‑1 (MQ=255)
                                 cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                      >  2:1192216/1‑150 (MQ=255)
                                 cAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGaa                                                                                                                      >  1:1195912/1‑150 (MQ=255)
                                  aGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAAt                                                                                                                     >  2:963149/1‑150 (MQ=255)
                                   gCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATc                                                                                                                    <  1:334098/150‑1 (MQ=255)
                                      cccGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCATTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCATCCTAGCGACCTCCCCAGTCAATCTGt                                                                                                                 >  2:1505095/1‑150 (MQ=255)
                                       ccGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGtt                                                                                                                <  2:63996/150‑1 (MQ=255)
                                                 tCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAtttt                                                                                                      <  1:416201/150‑1 (MQ=255)
                                                  ccTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttttt                                                                                                     >  1:1247247/1‑150 (MQ=255)
                                                  ccTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAttttt                                                                                                     >  1:370959/1‑150 (MQ=255)
                                                    tCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTa                                                                                                   <  1:813763/150‑1 (MQ=255)
                                                     cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTTAATCTGTTGCCTCATTTTTTAc                                                                                                  <  1:1026477/150‑1 (MQ=255)
                                                     cATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGGGAATCTGTTGCCTCATTTTTTAc                                                                                                  >  2:1094595/1‑150 (MQ=255)
                                                     cATTGCGCGATGCCTTCCAGCTTAATATCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTATTTAc                                                                                                  >  2:387297/1‑150 (MQ=255)
                                                       ttGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACAt                                                                                                <  2:587640/150‑1 (MQ=255)
                                                        tGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATa                                                                                               >  1:1379324/1‑150 (MQ=255)
                                                        tGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATa                                                                                               >  1:1469136/1‑150 (MQ=255)
                                                          cgcgATGCCTTCCAGCTTAATTTCTTTATATGCAATAGTTGCAGTTCATATCCGGGCTGCCTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGACTCCTCGCGACCTACCCAGTGAATCTGTTTCCTCATTTTTTAAATAca                                                                                             >  2:1077139/1‑149 (MQ=255)
                                                           gcgATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTg                                                                                            <  2:857304/150‑1 (MQ=255)
                                                              aTGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGACTCATTTTTTTCATACTGAGt                                                                                         >  1:240480/1‑150 (MQ=255)
                                                               tggcTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTa                                                                                        <  2:1603461/147‑1 (MQ=255)
                                                                gCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTAt                                                                                       >  1:690052/1‑150 (MQ=255)
                                                                   ttCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCg                                                                                    <  2:690052/150‑1 (MQ=255)
                                                                   ttCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCg                                                                                    >  1:877619/1‑150 (MQ=255)
                                                                   ttCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGAATGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACATCACCAGTGAATCTGTTGCATCATTTTTTACATACTGCGTATTCg                                                                                    >  1:1047511/1‑150 (MQ=255)
                                                                     ccAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGAc                                                                                  >  2:1140330/1‑150 (MQ=255)
                                                                      cAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACt                                                                                 <  1:1226680/150‑1 (MQ=255)
                                                                       aGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGGCTCATATTTTACATACTGCGTATTCGACtt                                                                                >  1:181514/1‑150 (MQ=255)
                                                                        gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTtc                                                                               >  1:1601318/1‑150 (MQ=255)
                                                                        gCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCAGTTTTTACATACTGCGTATTCGACTtc                                                                               >  2:992604/1‑150 (MQ=255)
                                                                            aaTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCTACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCa                                                                           <  1:1566108/150‑1 (MQ=255)
                                                                             aTTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCAc                                                                          >  1:1536947/1‑150 (MQ=255)
                                                                                tCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCGTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTg                                                                       >  1:736886/1‑150 (MQ=255)
                                                                                  tttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGtt                                                                     <  1:821551/150‑1 (MQ=255)
                                                                                  tttATTTGCAATAGTTGCAGTTCATATCCGGGCGGACTCCAGACTGGTAGTGAACTGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTCTACATACTGCGTATTCGACTTCTCCACCTGtt                                                                     <  1:1113409/150‑1 (MQ=255)
                                                                                   ttATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTg                                                                    <  2:23788/150‑1 (MQ=255)
                                                                                     aTTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTgcg                                                                  <  2:386713/150‑1 (MQ=255)
                                                                                     aTTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTACTCCACCTGTTgcg                                                                  >  2:237756/1‑150 (MQ=255)
                                                                                        tGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCaa                                                               <  2:1380135/150‑1 (MQ=255)
                                                                                         gCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAag                                                              <  2:1391262/150‑1 (MQ=255)
                                                                                            aTAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAgaga                                                           <  1:111547/150‑1 (MQ=255)
                                                                                              aGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGaaa                                                         >  2:790971/1‑150 (MQ=255)
                                                                                                  gCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTgg                                                     <  1:1599383/150‑1 (MQ=255)
                                                                                                  ccAGATCATATCCGGGCTGACTCCAGACTGGTAGGGAAATGCGCAATTTTTTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCGGTTGCCTCATTTTTAACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTgg                                                     <  1:640631/149‑1 (MQ=255)
                                                                                                   cAGTACATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCTCAATTTTGTAGAATTGCGCCACCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATAATGCGTATTCGAATTCTCCAAATGTTGCCCAATAGATACTggg                                                    >  2:791868/1‑150 (MQ=255)
                                                                                                    aGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGGGAAACTGGGt                                                   >  1:258365/1‑150 (MQ=255)
                                                                                                     gTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGtt                                                  <  2:877619/150‑1 (MQ=255)
                                                                                                       tCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTa                                                >  2:1158634/1‑150 (MQ=255)
                                                                                                       tCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTa                                                <  2:604720/150‑1 (MQ=255)
                                                                                                       tCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTCCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTa                                                >  2:1709808/1‑150 (MQ=255)
                                                                                                         atatCCGGGCTGACTCCAGACTGGGAGTGAAATGCGCAATTTTTTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTAtt                                              <  1:106269/150‑1 (MQ=255)
                                                                                                           atCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCa                                            >  2:1443378/1‑150 (MQ=255)
                                                                                                           atCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCa                                            <  1:464872/150‑1 (MQ=255)
                                                                                                             ccGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCAtt                                          <  1:208674/150‑1 (MQ=255)
                                                                                                                   ggACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCg                                    <  1:348312/149‑1 (MQ=255)
                                                                                                                     aCTCCAGTCTGGTAGTGAAATGCGCAATTTTGTAGAATTCCGCCTCCTCGCGACCTCCCCAGTGAATCTTTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAg                                  <  2:111443/150‑1 (MQ=255)
                                                                                                                     aCTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAg                                  <  2:74949/150‑1 (MQ=255)
                                                                                                                      cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAgg                                 >  2:1664269/1‑150 (MQ=255)
                                                                                                                      cTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGAGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTGGCGCAAGAGAAAATGGGTTTATTCATTTTTGAGAgg                                 >  1:1219352/1‑150 (MQ=255)
                                                                                                                       tCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGc                                <  2:1185774/150‑1 (MQ=255)
                                                                                                                        ccAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGcc                               <  1:1192216/150‑1 (MQ=255)
                                                                                                                         cAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCg                              >  1:87401/1‑150 (MQ=255)
                                                                                                                          aGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGa                             >  2:402756/1‑150 (MQ=255)
                                                                                                                               ggTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtct                        >  1:936752/1‑150 (MQ=255)
                                                                                                                               ggTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtct                        >  2:1546461/1‑150 (MQ=255)
                                                                                                                                gTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGActtctt                       >  2:411347/1‑150 (MQ=255)
                                                                                                                                 tAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttcttt                      <  1:1025706/150‑1 (MQ=255)
                                                                                                                                  aGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttc                     <  2:1245096/150‑1 (MQ=255)
                                                                                                                                  aGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACttctttc                     <  2:1621645/150‑1 (MQ=255)
                                                                                                                                       aaTGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGAc                >  2:1622123/1‑150 (MQ=255)
                                                                                                                                       aaTGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGAc                <  2:1536947/150‑1 (MQ=255)
                                                                                                                                         ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGCCTGCTTTCTGGACAg              >  1:1707925/2‑150 (MQ=255)
                                                                                                                                         ggcgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAg              >  1:1035092/2‑150 (MQ=255)
                                                                                                                                          gcgcAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGCAACTGGGTTTATTCATTTTTGAGAGGCCGACTTCTTTCTGGACAgg             >  2:569103/1‑150 (MQ=255)
                                                                                                                                           cgcAATTCTACAAAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATAATGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGa            >  2:590890/1‑150 (MQ=255)
                                                                                                                                             cAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACt          <  2:1379324/150‑1 (MQ=255)
                                                                                                                                                ttttGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTAATTTTTTACATACTGCGTATTCGCCTTCTCGACCTCTTGCGCAAGATAAACTGTGTTTATTCTTTTTTGAGAGGCAGACTTCTTTCGGTACAGGCCttga       >  2:311061/1‑148 (MQ=255)
                                                                                                                                                  ttGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATg     >  2:437365/1‑150 (MQ=255)
                                                                                                                                                   tGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATgc    >  2:1469371/1‑150 (MQ=255)
                                                                                                                                                    gTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAgg             >  2:1219060/1‑140 (MQ=255)
                                                                                                                                                     tAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATgcag  >  1:1517509/1‑150 (MQ=255)
                                                                                                                                                     tAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATgcag  >  1:1232515/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTGCCGCTGATTCTACGATTGCTTTTTACTGTCAGCACCCGGCAATCTTCCTCATTGCGCGATGCCTTCCAGCTTAATTTCTTTATTTGCAATAGTTGCAGTTCATATCCGGGCTGACTCCAGACTGGTAGTGAAATGCGCAATTTTGTAGAATTGCGCCTCCTCGCGACCTCCCCAGTGAATCTGTTGCCTCATTTTTTACATACTGCGTATTCGACTTCTCCACCTGTTGCGCAAGAGAAACTGGGTTTATTCATTTTTGCGAGGCCGACTTCTTTCTGGACAGGACTTTATGCAG  >  NC_000913/3700270‑3700568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: