Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,722,300 G→T 6.8% intergenic (+195/+28) sokA → / ← glyS pseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,722,3000GT 6.8% 592.7 / 18.6 189intergenic (+195/+28)sokA/glySpseudogene, sRNA antisense regulator blocking mokA/hokA translation/glycine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (90/85);  new base T (9/5);  total (99/90)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

ACCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGA  >  NC_000913/3722151‑3722446
                                                                                                                                                      |                                                                                                                                                  
aCCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAg                                                                                                                                                    <  1:866604/150‑1 (MQ=255)
 cccGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGa                                                                                                                                                   >  2:1292666/1‑150 (MQ=255)
 cccGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGACTGCCAGa                                                                                                                                                   >  1:568074/1‑150 (MQ=255)
    gTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATgg                                                                                                                                                <  2:1136559/150‑1 (MQ=255)
      ggCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGACAGATGGCa                                                                                                                                              >  1:259511/1‑150 (MQ=255)
         tATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGc                                                                                                                                           <  2:923731/150‑1 (MQ=255)
          aTTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCt                                                                                                                                          >  1:1502957/1‑150 (MQ=255)
           ttCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTa                                                                                                                                         >  1:1543682/1‑150 (MQ=255)
            tCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTAATCAGAAGAATATTAAAGGTTCCTGAATTATATAGCGATAAAAAAAGCCTGCCAGAAGTCAGGCAAt                                                                                                                                        >  1:120754/1‑150 (MQ=255)
              tAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCTtttt                                                                                                                                      <  1:444349/150‑1 (MQ=255)
               aGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTta                                                                                                                                     >  2:793241/1‑150 (MQ=255)
               aGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTta                                                                                                                                     >  1:936055/1‑150 (MQ=255)
               aGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTta                                                                                                                                     >  2:1012263/1‑150 (MQ=255)
                gCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaa                                                                                                                                    >  2:674453/1‑150 (MQ=255)
                gCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaa                                                                                                                                    >  1:1373880/1‑150 (MQ=255)
                gCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaa                                                                                                                                    <  2:446288/150‑1 (MQ=255)
                gCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTtaa                                                                                                                                    <  2:1574992/150‑1 (MQ=255)
                  ttAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCTttttttat                                                                                                                                  <  2:636097/150‑5 (MQ=255)
                     ataaGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCATCTGGCAGGCTttttttatcgc                                                                                                                               <  2:1384533/150‑8 (MQ=255)
                         gtttgtttCTTCTCCTAGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTATCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGt                                                                                                                           >  1:899640/1‑150 (MQ=255)
                           ttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGtta                                                                                                                         >  2:179941/1‑150 (MQ=255)
                           ttgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGtta                                                                                                                         <  1:1637377/150‑1 (MQ=255)
                            tgtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttat                                                                                                                        <  2:53739/150‑1 (MQ=255)
                            tgtttCTTATACTTGATATAAACCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGTTTGATGTATTATTTATAGATAAAAAAAGCATGCCATATGGAATGCTATATAATAACTGCTttat                                                                                                                        >  2:1367007/1‑150 (MQ=255)
                             gtttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatt                                                                                                                       >  2:1672417/1‑150 (MQ=255)
                              tttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatta                                                                                                                      >  1:972533/1‑150 (MQ=255)
                              tttCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGAGATAAAAAAATCCTGCCAGATGGCAGGCTATTTAATAACGGCGttatta                                                                                                                      >  1:982102/1‑150 (MQ=255)
                               ttcttcTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTtattat                                                                                                                     <  2:581841/150‑1 (MQ=255)
                                tcttcttctTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACgg                                                                                                                              >  2:1422703/1‑140 (MQ=255)
                                tcttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACTGCGTTattatt                                                                                                                    >  1:1029115/1‑150 (MQ=255)
                                 cttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGCCGttattaaat                                                                                                                   <  1:1106552/150‑4 (MQ=255)
                                  ttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGGTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGc                                                                                                                  <  2:169227/150‑1 (MQ=255)
                                  ttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGc                                                                                                                  <  1:236739/150‑1 (MQ=255)
                                  ttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGc                                                                                                                  <  2:1560039/150‑1 (MQ=255)
                                  ttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGc                                                                                                                  >  2:1479391/1‑150 (MQ=255)
                                  ttctCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGc                                                                                                                  <  1:1644780/150‑1 (MQ=255)
                                   tctcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCa                                                                                                                 <  2:303494/150‑1 (MQ=255)
                                   tctcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCa                                                                                                                 <  2:1172655/150‑1 (MQ=255)
                                    ctcCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGATGATTATTAAAGGTTGGTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATACAGGCGTTATTATTGCaa                                                                                                                >  1:113000/1‑150 (MQ=255)
                                    ctcCTTGATATAATCCTAAAAAAATCTCATAAAATAAATATATGAGAAAATCTATATTCAGCAGAAGATTATTAAAGTTTGCTATATTATTTAGCTATAAAAAAAGCCTGACAGATGGCAGGCTATTTAATAACGGCGTTATTATTGaaa                                                                                                                >  1:199400/1‑150 (MQ=255)
                                    atcCTTGATATAATACTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGCCGttattaaatagc                                                                                                                <  2:1321622/149‑7 (MQ=255)
                                      ccTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                              >  1:368175/1‑150 (MQ=255)
                                      ccTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                              <  1:1176511/150‑1 (MQ=255)
                                      ccTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                              >  2:829596/1‑150 (MQ=255)
                                      ccTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACa                                                                                                              >  2:715944/1‑150 (MQ=255)
                                      ccTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTAAGAAGAAGATTATTAAAGGTTGCTGTATTATATAGCGATAAAAAAAGCCTGCCAGATTGCAGGCTATTTAATAACTGCGTTTTTATTGCAACa                                                                                                              >  2:1331316/1‑150 (MQ=255)
                                         tGATATAATCCTAAAAAAATCACATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCTATAAAAAAAGCCTGCCAGATGACAGGCTATTTAATAACGGCGTTATTATTGAACAGCGa                                                                                                          >  2:1607054/1‑150 (MQ=255)
                                                 tCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATcc                                                                                                   <  1:698506/150‑1 (MQ=255)
                                                   cTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGc                                                                                                 >  2:1205790/1‑150 (MQ=255)
                                                   cTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGc                                                                                                 <  1:179941/150‑1 (MQ=255)
                                                    tAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCa                                                                                                <  1:1479391/150‑1 (MQ=255)
                                                      aaaaaaTCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGATGTATTATTTAGCGATAAAAAAAAGCATGCCAGATGGAAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCaa                                                                                               >  2:913310/1‑150 (MQ=255)
                                                               cATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGaa                                                                                     >  1:1002317/1‑150 (MQ=255)
                                                                  aaaaTTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAg                                                                                  <  2:442042/150‑1 (MQ=255)
                                                                      ttAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTcg                                                                              <  2:368175/150‑1 (MQ=255)
                                                                         atatatGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCgcgc                                                                           >  1:742150/1‑150 (MQ=255)
                                                                             atGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtt                                                                       <  1:1193323/150‑1 (MQ=255)
                                                                               gagaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGtttg                                                                     >  2:1157845/1‑149 (MQ=255)
                                                                               gagaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGttta                                                                     >  1:1197378/1‑149 (MQ=255)
                                                                               gagaTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTtc                                                                     >  2:100395/1‑150 (MQ=255)
                                                                                  aTAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCTACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTcc                                                                  <  1:560837/150‑1 (MQ=255)
                                                                                   tAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCa                                                                 <  2:1296308/150‑1 (MQ=255)
                                                                                         ttATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGG                                                           <  2:1649855‑M1/150‑1 (MQ=255)
                                                                                           aTTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCAAGATGGCAGGCTATTTAATAACGGCGTTATTATTTCAACAGCGAAATATCCGCAACGCGCAGTAAAAGTTCGCGCAGTTTCTCCATCATGGTC                                                         >  1:1043384‑M1/1‑150 (MQ=255)
                                                                                            ttCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCA                                                        >  1:1589242‑M1/1‑150 (MQ=255)
                                                                                            ttCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCA                                                        <  1:1597946‑M1/150‑1 (MQ=255)
                                                                                             tCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATATCGGCGTTTGTTTTGAAACAGCGAAATATCCGCAACGCGAAGGAACAGTTGGCGCATTTACTCCAGAATGGTCAa                                                       >  1:107126‑M1/1‑149 (MQ=255)
                                                                                                   gaagaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGtt                                                 <  1:984136/150‑1 (MQ=255)
                                                                                                      gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                              <  2:553861/150‑1 (MQ=255)
                                                                                                      gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                              <  1:1659614/150‑1 (MQ=255)
                                                                                                      gaTTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGAt                                              <  1:1685566/150‑1 (MQ=255)
                                                                                                                   tGCTGTATTATTTAGCGATAAAAATAGCCTGCCAGATGGCAGTCTATTTAATAACTGCGTTATTATTGCAACAGCGAAATATCAGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTg                                 <  2:1226334/150‑1 (MQ=255)
                                                                                                                   tGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTAATACGCAATTCTTTg                                 <  2:972533/150‑1 (MQ=255)
                                                                                                                     cTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtc                               >  1:1230528/1‑150 (MQ=255)
                                                                                                                     cTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGGCAGACGGTTGATACGCAATTCTTTGtc                               <  2:205596/150‑1 (MQ=255)
                                                                                                                       gTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGGAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGtcat                             >  1:133304/1‑150 (MQ=255)
                                                                                                                            attTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAAcc                        <  2:936055/150‑1 (MQ=255)
                                                                                                                               tAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGAGAAATATCCGCAACGCGCAGGAAAAGTTCTCGCAGTTTCTCCAGCATTGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtg                     <  1:1039390‑M2/150‑3 (MQ=255)
                                                                                                                                 gCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgac                   >  2:1549993‑M2/1‑146 (MQ=255)
                                                                                                                                 gCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgac                   >  2:259511‑M2/1‑146 (MQ=255)
                                                                                                                                  cGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgacc                  >  2:344903‑M2/1‑145 (MQ=255)
                                                                                                                                   gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAtggttgatgacaaagaattgcgtatcaaccgtttgacca                 >  1:390427‑M1/1‑111 (MQ=255)
                                                                                                                                   gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgacca                 >  1:674453‑M2/1‑144 (MQ=255)
                                                                                                                                   gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgacca                 <  1:1555132‑M2/150‑7 (MQ=255)
                                                                                                                                   gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgacca                 >  2:1496831‑M2/1‑144 (MQ=255)
                                                                                                                                   gATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTACAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCAtgacca                 <  2:1082959‑M2/150‑7 (MQ=255)
                                                                                                                                    aTAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAtggttgatgacaaagaattgcgtatcaaccgtctgaccat                <  2:1448888‑M1/150‑41 (MQ=255)
                                                                                                                                     tAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGCAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATc               >  1:1159162/1‑150 (MQ=255)
                                                                                                                                     tAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATc               <  1:100395/150‑1 (MQ=255)
                                                                                                                                       ttaaataGCCTGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCa              >  2:491392/3‑150 (MQ=255)
                                                                                                                                        aaaaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCAc             >  2:1317528/1‑150 (MQ=255)
                                                                                                                                          aaaaGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAtggttgatgacaaagaattgcgtatcaaccgtttgaccatgctgg           <  2:390427‑M1/150‑46 (MQ=255)
                                                                                                                                               ccTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACTGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATca      <  2:982102/150‑2 (MQ=255)
                                                                                                                                                 tGCCATCTGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa    >  2:633227/1‑150 (MQ=255)
                                                                                                                                                 tGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaa    >  2:404273/1‑150 (MQ=255)
                                                                                                                                                  gCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaag   <  1:807129/150‑1 (MQ=255)
                                                                                                                                                   ccAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGCCCATCACTTTATCgaaga  >  2:1058265/1‑150 (MQ=255)
                                                                                                                                                   ccAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaaga  >  2:187917/1‑150 (MQ=255)
                                                                                                                                                   ccAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaaga  >  2:882631/1‑150 (MQ=255)
                                                                                                                                                   ccAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTACAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCgaaga  >  1:77342/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
ACCCGTGGCTATTCTAGCTTAATAAGTTTGTTTCTTCTCCTTGATATAATCCTAAAAAAATCTCATAAAATTAATATATGAGATAATCTTTATTCAGCAGAAGATTATTAAAGGTTGCTGTATTATTTAGCGATAAAAAAAGCCTGCCAGATGGCAGGCTATTTAATAACGGCGTTATTATTGCAACAGCGAAATATCCGCAACGCGCAGGAACAGTTCGCGCAGTTTCTCCAGCATGGTCAGACGGTTGATACGCAATTCTTTGTCATCAACCATGACCATCACTTTATCGAAGA  >  NC_000913/3722151‑3722446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: