Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,728,217	A→T	6.8%	I385I (ATT→ATA) ‡	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,217	0	A	T	6.8%	206.8 / 2.5	71	I385I (ATT→ATA) ‡	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base A (37/29);  new base T (2/3);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTC  >  NC_000913/3728070‑3728366                                                                                                                                                    |                                                                                                                                                      gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAt                                                                                                                                                     <  2:1051594/150‑1 (MQ=255)   tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAc                                                                                                                                                   <  2:1096164/150‑1 (MQ=255)    cTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATAcc                                                                                                                                                  <  1:139892/150‑1 (MQ=255)          cAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                                                                                                                                            <  1:1411825/150‑1 (MQ=255)          cAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaag                                                                                                                                            <  2:999183/150‑4 (MQ=255)           aGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaagt                                                                                                                                           <  1:1383947/150‑5 (MQ=255)            gCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCTATCAACGTAACACTTTTCGGTATTAAACCGCaaagtt                                                                                                                                          <  1:1477103/150‑6 (MQ=255)                      gcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATc                                                                                                                                <  2:426496/150‑1 (MQ=255)                              cTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt                                                                                                                        <  2:1193967/150‑1 (MQ=255)                                     cacaTCCCCCCCCGTACTGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAg                                                                                                                 <  2:1310478/150‑1 (MQ=255)                                         tCCCCCCCCGTACGGTAGTCGAGCTGCTGACCGCGGGTGTCCGCCCGCCTCGGCCGCCCGTACTCCCGACGCGCCCCGCCCCCAATCAACGTAACACTTTGGGGTTTTATCCCGCAGCGCTGGACGCCATACATGCCAGCTGCCAgcggg                                                                                                             >  2:1557932/1‑148 (MQ=255)                                            ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCACCGTAACCCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCagtc                                                                                                          >  1:687406/1‑147 (MQ=255)                                              ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc                                                                                                        <  1:712258/150‑1 (MQ=255)                                                    aCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGcc                                                                                                  <  2:188250/150‑1 (MQ=255)                                                         aaTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCa                                                                                             >  2:622674/1‑150 (MQ=255)                                                          aTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAg                                                                                            >  1:1560124/1‑150 (MQ=255)                                                            cGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCCGGCATGCACGACATCCGTGCCATCTGCCAGCGCATAACCCACGCCGTCCAGCa                                                                                          >  2:1497293/1‑150 (MQ=255)                                                             gAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCAc                                                                                         >  2:1021779/1‑150 (MQ=255)                                                               gctgctGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTg                                                                                       >  1:991269/1‑150 (MQ=255)                                                                     gACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCCATCTGCCCGCGCATAACCCACGCCTTCCAGCACTGCTcgcg                                                                                 >  1:58202/1‑150 (MQ=255)                                                                     gACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTcgcg                                                                                 >  1:1260185/1‑150 (MQ=255)                                                                         gCTGATATCCGCCAGCATCTGACGCCAATACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAg                                                                             >  2:100486/1‑150 (MQ=255)                                                                          cTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACGCTTTGCGGTATACTACCGCAGGCATGCCCGACATCCATGCCATCTGCCCGCGCATAACCCACGCCTTCCAGCCCTGCGCGCGCCAgg                                                                            >  1:339358/1‑149 (MQ=255)                                                                          cTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGt                                                                            >  2:1649541/1‑150 (MQ=255)                                                                           tGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGtt                                                                           >  2:719831/1‑150 (MQ=255)                                                                             atatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCa                                                                         >  2:1558724/1‑150 (MQ=255)                                                                             atatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCa                                                                         >  2:1613735/1‑150 (MQ=255)                                                                              tatCCGCCAGTATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAt                                                                        <  1:313210/150‑1 (MQ=255)                                                                              tatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCCTCACCCACGCCTTCCCGCACTGCTCGCGCCAGTTCAt                                                                        >  1:547312/1‑150 (MQ=255)                                                                              tatCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAt                                                                        >  1:554092/1‑150 (MQ=255)                                                                               atCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCAtt                                                                       >  1:1279143/1‑150 (MQ=255)                                                                                tCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTg                                                                      >  2:1476937/1‑150 (MQ=255)                                                                                   gCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCACCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTgggg                                                                   <  2:228540/150‑1 (MQ=255)                                                                                   gCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTgggg                                                                   <  2:436407/150‑1 (MQ=255)                                                                                    ccAGCATCTGCCGCCAGTGCTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCTCGACATCCATGCCATCGGCCAGCGCATAACCCCCGCGTTCCAGCCCTGCTCGCGCCAGGTCATTGGGGc                                                                  >  2:161216/1‑150 (MQ=255)                                                                                      aGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCa                                                                <  1:1021779/150‑1 (MQ=255)                                                                                         aTCTGACTCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGt                                                             <  2:1684360/150‑1 (MQ=255)                                                                                         aTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGt                                                             <  1:868300/150‑1 (MQ=255)                                                                                                gCCAGGACTCACTCCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATTCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGGGCATTGGGGCCATGTTGATGAg                                                      <  2:1147876/150‑1 (MQ=255)                                                                                                  cAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCCGGCCAGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTc                                                    >  1:614180/1‑150 (MQ=255)                                                                                                    gTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCaa                                                  >  1:391770/1‑150 (MQ=255)                                                                                                      gcTCACTACGCGCCCCGCCCCCAATCATCGTAACACTTTGCGGTGTAATACCGCAGGCATGCACGACTTCCATGCCATCTGCCAGCGCATAACCCACGCATTCCAGCACTTCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAc                                                <  2:1553816/149‑1 (MQ=255)                                                                                                       cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTACAACCGCAGGCATGCACGACATCCATGCCATCTGCCTGCGCATAACCCACGCCTTCCCGCACTGCTCGCGCCAGTTCCTTGGGGCCATGTTGATGAGTCCAAca                                               >  1:1553816/1‑149 (MQ=255)                                                                                                       cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCCCGACATCCATGCCATCTGCCAGCGCATACCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAcc                                               >  1:1172823/1‑150 (MQ=255)                                                                                                       cTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCATTTCATTGGGGCCATGTTGATGAGTCAAAcc                                               <  1:1658413/150‑1 (MQ=255)                                                                                                          aCTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGCCATGCACGACATCCATGCCATCTGCCAGCGCATACCCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaaa                                            >  1:36022/1‑150 (MQ=255)                                                                                                             aCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGaa                                         <  1:200284/150‑1 (MQ=255)                                                                                                             aCGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTAAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGaa                                         <  1:205896/150‑1 (MQ=255)                                                                                                                cgccccgcccCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAc                                      <  1:1380317/150‑1 (MQ=255)                                                                                                                 gccccgccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAcc                                     >  2:1422136/1‑150 (MQ=255)                                                                                                                      gccccCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCtt                                >  2:1236417/1‑150 (MQ=255)                                                                                                                       cccccAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCTGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTc                               >  1:929783/1‑150 (MQ=255)                                                                                                                           cAATCAACGTAACGCTTTGCGGTTTAATACCGCAGGCAGGCACGACATCCATGCCATCTGCCAGCGAATAACCCCCGCCTTACAGCACTGCTCGCTCCAGTTCAGTGGGGCCAGGTTGATTAGGCAAACCAAAGAAATCCCCCTGCGccg                           >  2:883889/1‑149 (MQ=255)                                                                                                                           cAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCt                           <  2:1560124/150‑1 (MQ=255)                                                                                                                            aaTCCACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTg                          >  1:1292129/1‑150 (MQ=255)                                                                                                                            aaTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTg                          <  1:1558724/150‑1 (MQ=255)                                                                                                                             aTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTgg                         <  1:622674/150‑1 (MQ=255)                                                                                                                             aTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTgg                         <  1:1356210/150‑1 (MQ=255)                                                                                                                               cAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTgggg                       <  1:1641700/150‑1 (MQ=255)                                                                                                                                 aCGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGat                     >  2:1715136/1‑150 (MQ=255)                                                                                                                                  cGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGACAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGatt                    >  2:1339766/1‑150 (MQ=255)                                                                                                                                   gTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATTCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGatta                   <  1:1557932/150‑1 (MQ=255)                                                                                                                                    tAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGattat                  >  1:958994/1‑150 (MQ=255)                                                                                                                                       cacTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgt               <  1:1613735/150‑1 (MQ=255)                                                                                                                                          tttGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg            <  1:810963/150‑1 (MQ=255)                                                                                                                                          cctGCGGTATTAAACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCAGGTTGATGAGTCAACCCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgtg            >  2:316227/3‑150 (MQ=255)                                                                                                                                               ggTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAACACCCCCTTCGCCTGGGGATTATTGTGTGGCGTa       >  2:922838/1‑150 (MQ=255)                                                                                                                                                gTTTAATACCGCAGGCATGCACGACATCCATGCCATCTACCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTAc      >  2:1063804/1‑150 (MQ=255)                                                                                                                                                 tttAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACg     >  1:806793/1‑150 (MQ=255)                                                                                                                                                   tAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCt   >  1:1556537/1‑150 (MQ=255)                                                                                                                                                   tAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCt   >  2:1264290/1‑150 (MQ=255)                                                                                                                                                    aaTACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAATCCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTc  >  2:442246/1‑150 (MQ=255)                                                                                                                                                    aaTACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTc  >  2:752818/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTC  >  NC_000913/3728070‑3728366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑