Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,732,139 G→T 5.9% intergenic (+16/‑62) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,1390GT 5.9% 345.8 / 2.4 112intergenic (+16/‑62)xylF/xylGD‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base G (50/54);  new base T (2/6);  total (52/60)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCT  >  NC_000913/3731990‑3732287
                                                                                                                                                     |                                                                                                                                                    
cAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAgcg                                                                                                                                                      >  2:1575752/1‑150 (MQ=255)
  aaaGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGcgcg                                                                                                                                                    <  1:1025623/150‑1 (MQ=255)
     gCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAg                                                                                                                                                 <  2:653044/150‑1 (MQ=255)
      cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                >  2:821655/1‑150 (MQ=255)
      cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                <  1:1012889/150‑1 (MQ=255)
      cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                >  2:197434/1‑150 (MQ=255)
      cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGCTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                >  1:1084060/1‑150 (MQ=255)
      cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCATCCCGCCTCATGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                >  2:1322645/1‑150 (MQ=255)
          tACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCgggg                                                                                                                                            <  2:1476173/150‑1 (MQ=255)
           aacacaCTGAATAATGGCCGGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACATCCTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCggggg                                                                                                                                           <  2:1324265/148‑1 (MQ=255)
            ccACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGc                                                                                                                                          >  1:447737/1‑150 (MQ=255)
            ccACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGc                                                                                                                                          >  2:1446722/1‑150 (MQ=255)
             cacaCTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCg                                                                                                                                         <  2:1681393/150‑1 (MQ=255)
              acacTGAATACTGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCGGGGGCGt                                                                                                                                        <  1:995784/150‑1 (MQ=255)
              acacTGAATAATGGCCTGAAAGATCTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGt                                                                                                                                        <  1:1123763/150‑1 (MQ=255)
                acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCTAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTATGCGTTACGCCCCAGCGCGGCGCGGGGGCGTGa                                                                                                                                      >  2:674408/1‑150 (MQ=255)
                acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaa                                                                                                                                      <  1:406863/150‑3 (MQ=255)
                 cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                     >  1:318839/1‑150 (MQ=255)
                 cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                     <  1:1601665/150‑4 (MQ=255)
                 atGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                     <  2:1664841/149‑1 (MQ=255)
                  tGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAGGTGAATAAAAACAACATCAAAGATACCGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaacg                                                                                                                                    <  1:901176/150‑5 (MQ=255)
                  cgAATACTGGCCTGAAAGATGTCCCCTCCCCCCTCCTGACACCGATCGATGTCAAAAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAtt                                                                                                                                    <  1:669082/149‑1 (MQ=255)
                   gAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAttt                                                                                                                                   <  1:1446722/150‑1 (MQ=255)
                      taatTGCCTGAAAGATGGCCCCGCCCGCCTCCTGCCCCCGCTCGATGTGAATAAAAACAACATCAAAGATACGGTACTTAAAGACGGATTCCACACCGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctc                                                                                                                                <  1:1482605/150‑1 (MQ=255)
                      taatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACATCATCAAAGATACGGTAATTTAAGACGGATTCCACAAAGAGAGCGTGCTGTAAGCGTTACGAACCAGCGCGGAGCGGGGGCGTGATGtctc                                                                                                                                >  1:708164/1‑150 (MQ=255)
                       aatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACATAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctct                                                                                                                               <  1:649376/150‑1 (MQ=255)
                         tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                             <  2:860289/150‑1 (MQ=255)
                         gcgCCTGAAAGATGTCCCATCCCGCCTCCTCACACCGATGGATGTCAATAAAAACAACATCAAAGCTACGGTAATTAAAGACGGATTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCTGCGCGGAGCGTGGGCGTGATTTCTCTcc                                                                                                                             <  2:830540/148‑1 (MQ=255)
                           gCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCAt                                                                                                                           <  2:934239/150‑1 (MQ=255)
                            ccTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAATGAGAGCGAGATGAAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATg                                                                                                                          >  2:224035/1‑150 (MQ=255)
                            ccTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATg                                                                                                                          >  2:699738/1‑150 (MQ=255)
                             cTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGc                                                                                                                         >  1:1087933/1‑150 (MQ=255)
                               gAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcg                                                                                                                       >  2:1484403/1‑150 (MQ=255)
                                 aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                     >  1:1703851/1‑150 (MQ=255)
                                 aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                     <  1:504240/150‑1 (MQ=255)
                                 aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                     <  2:1290274/150‑1 (MQ=255)
                                 aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                     >  1:795733/1‑150 (MQ=255)
                                   gATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                   <  2:1278397/150‑1 (MQ=255)
                                   gATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                   >  2:450770/1‑150 (MQ=255)
                                   caTGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGCCGGATGCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                   <  1:248633/149‑1 (MQ=255)
                                        ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                              >  1:1120437/1‑150 (MQ=255)
                                        ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                              >  2:734709/1‑150 (MQ=255)
                                        cccataccGCCTCCTGACACCGAACGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGTTTCCACAAAGAGAGCGAGATGTAAGCGTTACGCCCCAGCGCTGAGCGGGGGCGAGATTTCTCTCCATGCCGCGtgaatga                                                                                                              >  2:1233021/7‑150 (MQ=255)
                                            tCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTg                                                                                                          >  1:1551650/1‑150 (MQ=255)
                                             cccGCCTCCTGACACCTATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTgg                                                                                                         <  2:795733/150‑1 (MQ=255)
                                                   tcctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAgg                                                                                                   >  2:1345803/1‑150 (MQ=255)
                                                        acacCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAg                                                                                              <  1:225568/150‑1 (MQ=255)
                                                           ccGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCg                                                                                           >  2:1382513/1‑150 (MQ=255)
                                                            cgatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGt                                                                                          <  2:753787/150‑1 (MQ=255)
                                                             gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                         >  2:360187/1‑150 (MQ=255)
                                                             gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                         <  1:1281882/150‑1 (MQ=255)
                                                                 gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                             >  2:706781/1‑142 (MQ=255)
                                                                 gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                             >  2:132594/1‑142 (MQ=255)
                                                                 gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                             <  1:132594/142‑1 (MQ=255)
                                                                 gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                             <  1:706781/142‑1 (MQ=255)
                                                                 gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGc                                                                                     >  2:1494997/1‑150 (MQ=255)
                                                                   tgtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGTGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                                   <  2:1712061/150‑1 (MQ=255)
                                                                   tgtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                                   <  1:1345803/150‑1 (MQ=255)
                                                                      gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                                >  1:497212/1‑150 (MQ=255)
                                                                       aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCATCGCGGAGCGGGGGCGTGATTTCTCTCCATGCAGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGccgaatc                                                                               >  2:1253748/1‑145 (MQ=255)
                                                                       aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATc                                                                               >  2:505675/1‑150 (MQ=255)
                                                                        aTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                              <  2:747354/150‑1 (MQ=255)
                                                                           aaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                           >  1:448184/1‑150 (MQ=255)
                                                                            aaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGAGTGAATGAATTGGCTTAGGTGGAGACGTTATGCCTTATCTACtt                                                                          >  2:80057/1‑150 (MQ=255)
                                                                             aacaacATCAAAGATACTGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTg                                                                         <  2:1703851/150‑1 (MQ=255)
                                                                                 acaTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAAt                                                                     <  1:1024036/150‑1 (MQ=255)
                                                                                  caTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAATTg                                                                    <  1:54111/150‑1 (MQ=255)
                                                                                    tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                                  <  1:544700/150‑1 (MQ=255)
                                                                                    tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                                  >  1:564280/1‑150 (MQ=255)
                                                                                      aaaGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                                <  1:496698/150‑1 (MQ=255)
                                                                                        aGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAAc                                                              <  1:588137/150‑1 (MQ=255)
                                                                                             cGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAc                                                         <  1:836153/150‑1 (MQ=255)
                                                                                              ggaaaTTATAGACGGATTCCACAAAGAGAGCGAGCGGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAcc                                                        <  2:1517883/147‑1 (MQ=255)
                                                                                               gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                       >  1:282283/1‑150 (MQ=255)
                                                                                                tAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCaa                                                      <  2:318839/150‑1 (MQ=255)
                                                                                                 aaTTAAAGACGGATTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAGTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAATAACATTACaaaa                                                     >  2:186917/1‑150 (MQ=255)
                                                                                                    tAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGACGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAcc                                                  <  1:699738/150‑1 (MQ=255)
                                                                                                     aaaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCt                                                 <  2:447737/150‑1 (MQ=255)
                                                                                                      aaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCtt                                                >  2:919745/1‑150 (MQ=255)
                                                                                                       aGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTc                                               <  1:409318/150‑1 (MQ=255)
                                                                                                          cGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTACATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGGCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGc                                            >  2:261990/1‑150 (MQ=255)
                                                                                                          cGGATTCCACAAAGAGAGCGACCTGACAGCGTTACGCGCCAGCGCGGAGCGGGGGCGGGATTTCTCTCCATGCCGCGGGAATGAAATGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGc                                            <  2:906878/150‑1 (MQ=255)
                                                                                                           ggATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCa                                           >  1:1557828/1‑150 (MQ=255)
                                                                                                            gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAg                                          <  1:821655/150‑1 (MQ=255)
                                                                                                            gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAg                                          <  1:1322645/150‑1 (MQ=255)
                                                                                                              ttCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgtg                                        <  2:497212/150‑1 (MQ=255)
                                                                                                                ccACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGTGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGtgtg                                      >  2:1016965/1‑150 (MQ=255)
                                                                                                                 cacaAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGa                                     >  2:519160/1‑150 (MQ=255)
                                                                                                                  aaaaaGAGAGCTAGCTGTAAGCGTTACGCCCCAGCGGGGAGGGGGGGAGTGTTTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGaa                                    <  2:1084060/148‑1 (MQ=255)
                                                                                                                   caAAGAGAGGGAGCTGTATGGGTTACGCGCCATCTTGGAGCGGGGGCGTGATTTCTCTCCATGCCGCTTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATTAAGAACATTACCAAAACCTTCGGCAGTGTGAAg                                   <  1:1263599/150‑1 (MQ=255)
                                                                                                                                cTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGAGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACg                      >  2:921518/1‑150 (MQ=255)
                                                                                                                                  gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                    <  2:1238203/150‑1 (MQ=255)
                                                                                                                                  gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                    >  2:1149290/1‑150 (MQ=255)
                                                                                                                                  gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                    <  1:919745/150‑1 (MQ=255)
                                                                                                                                          caCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATATACTTGAAATGAAGAACATTAACAAAACCTTAGGCAGTGTGAAGGCGATTGATAACGTCTGCTTTCg            >  2:1499811/2‑150 (MQ=255)
                                                                                                                                            cGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGt          >  2:1236848/1‑150 (MQ=255)
                                                                                                                                              ccccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTg        <  2:1557828/150‑1 (MQ=255)
                                                                                                                                              ccccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTACGGCAGTGTGAAGGCGATTGATAACGTCTGCTGGCGGTTg        >  2:346143/1‑150 (MQ=255)
                                                                                                                                                ccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGaa      <  2:940046/150‑1 (MQ=255)
                                                                                                                                                    cgcgGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCt  <  1:360187/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCT  >  NC_000913/3731990‑3732287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: