Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,732,143 A→C 6.7% intergenic (+20/‑58) xylF → / → xylG D‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,732,1430AC 6.7% 316.8 / 11.8 116intergenic (+20/‑58)xylF/xylGD‑xylose transporter subunit/D‑xylose ABC transporter dual domain ATPase
Reads supporting (aligned to +/- strand):  ref base A (50/57);  new base C (3/5);  total (53/63)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

Only 100 of 116 total aligned reads displayed.

GCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCG  >  NC_000913/3731995‑3732291
                                                                                                                                                    |                                                                                                                                                    
gCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGCTACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAg                                                                                                                                                     <  2:1297383/150‑1 (MQ=255)
gCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAg                                                                                                                                                     <  2:653044/150‑1 (MQ=255)
 cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                    >  2:821655/1‑150 (MQ=255)
 cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                    <  1:1012889/150‑1 (MQ=255)
 cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                    >  2:197434/1‑150 (MQ=255)
 cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGCTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                    >  1:1084060/1‑150 (MQ=255)
 cAGATACCACACTGAATAATGGCCTGAAAGATGTCCCATCCCGCCTCATGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGc                                                                                                                                                    >  2:1322645/1‑150 (MQ=255)
     tACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCgggg                                                                                                                                                <  2:1476173/150‑1 (MQ=255)
       ccACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGc                                                                                                                                              >  1:447737/1‑150 (MQ=255)
       ccACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGc                                                                                                                                              >  2:1446722/1‑150 (MQ=255)
         acacTGAATACTGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCGGGGGCGt                                                                                                                                            <  1:995784/150‑1 (MQ=255)
         acacTGAATAATGGCCTGAAAGATCTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGt                                                                                                                                            <  1:1123763/150‑1 (MQ=255)
           acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCTAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTATGCGTTACGCCCCAGCGCGGCGCGGGGGCGTGa                                                                                                                                          >  2:674408/1‑150 (MQ=255)
           acTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaa                                                                                                                                          <  1:406863/150‑3 (MQ=255)
            cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                         >  1:318839/1‑150 (MQ=255)
            cTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaac                                                                                                                                         <  1:1601665/150‑4 (MQ=255)
            atGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAt                                                                                                                                         <  2:1664841/149‑1 (MQ=255)
             tGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAGGTGAATAAAAACAACATCAAAGATACCGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCCGCTCCGCGCTGGGGCGtaacg                                                                                                                                        <  1:901176/150‑5 (MQ=255)
             cgAATACTGGCCTGAAAGATGTCCCCTCCCCCCTCCTGACACCGATCGATGTCAAAAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAtt                                                                                                                                        <  1:669082/149‑1 (MQ=255)
              gAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAttt                                                                                                                                       <  1:1446722/150‑1 (MQ=255)
                 taatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACATCATCAAAGATACGGTAATTTAAGACGGATTCCACAAAGAGAGCGTGCTGTAAGCGTTACGAACCAGCGCGGAGCGGGGGCGTGATGtctc                                                                                                                                    >  1:708164/1‑150 (MQ=255)
                 taatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctc                                                                                                                                    >  1:1238203/1‑150 (MQ=255)
                  aatGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACATAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTtctct                                                                                                                                   <  1:649376/150‑1 (MQ=255)
                    tGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTcc                                                                                                                                 <  2:860289/150‑1 (MQ=255)
                    gcgCCTGAAAGATGTCCCATCCCGCCTCCTCACACCGATGGATGTCAATAAAAACAACATCAAAGCTACGGTAATTAAAGACGGATTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCTGCGCGGAGCGTGGGCGTGATTTCTCTcc                                                                                                                                 <  2:830540/148‑1 (MQ=255)
                      gCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCAt                                                                                                                               <  2:934239/150‑1 (MQ=255)
                       ccTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAATGAGAGCGAGATGAAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATg                                                                                                                              >  2:224035/1‑150 (MQ=255)
                       ccTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATg                                                                                                                              >  2:699738/1‑150 (MQ=255)
                        cTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGc                                                                                                                             >  1:1087933/1‑150 (MQ=255)
                            aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                         <  1:504240/150‑1 (MQ=255)
                            aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                         >  1:795733/1‑150 (MQ=255)
                            aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                         <  2:1290274/150‑1 (MQ=255)
                            aaGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCcgcg                                                                                                                         >  1:1703851/1‑150 (MQ=255)
                              gATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                       >  2:450770/1‑150 (MQ=255)
                              gATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                       <  2:1278397/150‑1 (MQ=255)
                              caTGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGCCGGATGCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtg                                                                                                                       <  1:248633/149‑1 (MQ=255)
                                   ccccTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGtgaatga                                                                                                                  >  1:1120437/1‑150 (MQ=255)
                                       tCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTg                                                                                                              >  1:1551650/1‑150 (MQ=255)
                                        cccGCCTCCTGACACCTATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTgg                                                                                                             <  2:795733/150‑1 (MQ=255)
                                         ccGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGc                                                                                                            <  2:712027/150‑1 (MQ=255)
                                          cGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCt                                                                                                           >  2:255695/1‑150 (MQ=255)
                                              tcctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAgg                                                                                                       >  2:1345803/1‑150 (MQ=255)
                                               cctGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGt                                                                                                      >  2:492803/1‑150 (MQ=255)
                                                      ccGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCg                                                                                               >  2:1382513/1‑150 (MQ=255)
                                                       cgatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGt                                                                                              <  2:753787/150‑1 (MQ=255)
                                                        gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                             <  1:1281882/150‑1 (MQ=255)
                                                        gatcgatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGtt                                                                                             >  2:360187/1‑150 (MQ=255)
                                                            gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                                 >  2:706781/1‑142 (MQ=255)
                                                            gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                                 <  1:132594/142‑1 (MQ=255)
                                                            gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGt                                                                                                 >  2:132594/1‑142 (MQ=255)
                                                            gatGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGc                                                                                         >  2:1494997/1‑150 (MQ=255)
                                                              tgtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGTGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                                       <  2:1712061/150‑1 (MQ=255)
                                                              tgtgAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCt                                                                                       <  1:1345803/150‑1 (MQ=255)
                                                                 gAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTAt                                                                                    >  1:497212/1‑150 (MQ=255)
                                                                  aaTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCATCGCGGAGCGGGGGCGTGATTTCTCTCCATGCAGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGccgaatc                                                                                   >  2:1253748/1‑145 (MQ=255)
                                                                   aTAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCt                                                                                  <  2:747354/150‑1 (MQ=255)
                                                                      aaaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACt                                                                               >  1:448184/1‑150 (MQ=255)
                                                                       aaaCAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGAGTGAATGAATTGGCTTAGGTGGAGACGTTATGCCTTATCTACtt                                                                              >  2:80057/1‑150 (MQ=255)
                                                                        aacaacATCAAAGATACTGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTg                                                                             <  2:1703851/150‑1 (MQ=255)
                                                                            acaTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAAt                                                                         <  1:1024036/150‑1 (MQ=255)
                                                                             caTCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAATTg                                                                        <  1:54111/150‑1 (MQ=255)
                                                                              aTCAAAGATCCGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATga                                                                       <  1:486457/150‑1 (MQ=255)
                                                                               tCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaa                                                                      >  1:564280/1‑150 (MQ=255)
                                                                                 aaaGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATgaaga                                                                    <  1:496698/150‑1 (MQ=255)
                                                                                   aGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAAc                                                                  <  1:588137/150‑1 (MQ=255)
                                                                                        cGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAc                                                             <  1:836153/150‑1 (MQ=255)
                                                                                        cGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCGTAGGAGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAc                                                             <  1:257868/150‑1 (MQ=255)
                                                                                         ggaaaTTATAGACGGATTCCACAAAGAGAGCGAGCGGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTAcc                                                            <  2:1517883/147‑1 (MQ=255)
                                                                                          gTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCa                                                           >  1:282283/1‑150 (MQ=255)
                                                                                           tAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCaa                                                          <  2:318839/150‑1 (MQ=255)
                                                                                            aaTTAAAGACGGATTCCACAAAGAGAGCGCGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGAGTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAATAACATTACaaaa                                                         >  2:186917/1‑150 (MQ=255)
                                                                                             aTTAAAGACGGATTCCACAAAGAGAGCGAGCTGTACGCGTTACGCCCAAGCGCGGAGCGGGGGCGTGATTTCTAGCCATGCCGCGTGAAGGAATTGGCTTAGGTGTAGTCGTTATGCCTTATCTACTTGAAATGAAGAAAATTACCaaaa                                                        >  1:814060/1‑150 (MQ=255)
                                                                                               tAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGACGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAAcc                                                      <  1:699738/150‑1 (MQ=255)
                                                                                                aaaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCt                                                     <  2:447737/150‑1 (MQ=255)
                                                                                                 aaGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCtt                                                    >  2:919745/1‑150 (MQ=255)
                                                                                                     cGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGc                                                <  1:1484403/150‑1 (MQ=255)
                                                                                                     cGGATTCCACAAAGAGAGCGACCTGACAGCGTTACGCGCCAGCGCGGAGCGGGGGCGGGATTTCTCTCCATGCCGCGGGAATGAAATGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGc                                                <  2:906878/150‑1 (MQ=255)
                                                                                                      ggATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCa                                               >  1:1557828/1‑150 (MQ=255)
                                                                                                       gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAg                                              <  1:821655/150‑1 (MQ=255)
                                                                                                       gATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAg                                              <  1:1322645/150‑1 (MQ=255)
                                                                                                         ttCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAgtg                                            <  2:497212/150‑1 (MQ=255)
                                                                                                           ccACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGTGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGtgtg                                          >  2:1016965/1‑150 (MQ=255)
                                                                                                            cacaAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGa                                         >  2:519160/1‑150 (MQ=255)
                                                                                                             acaAAGCTAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGaa                                        <  2:163025/150‑1 (MQ=255)
                                                                                                             aaaaaGAGAGCTAGCTGTAAGCGTTACGCCCCAGCGGGGAGGGGGGGAGTGTTTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGaa                                        <  2:1084060/148‑1 (MQ=255)
                                                                                                              caAAGAGAGGGAGCTGTATGGGTTACGCGCCATCTTGGAGCGGGGGCGTGATTTCTCTCCATGCCGCTTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATTAAGAACATTACCAAAACCTTCGGCAGTGTGAAg                                       <  1:1263599/150‑1 (MQ=255)
                                                                                                                       cgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGAt                              >  1:608943/1‑150 (MQ=255)
                                                                                                                       cgagcTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTAGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGAt                              >  2:1472784/1‑150 (MQ=255)
                                                                                                                           cTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGAGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACg                          >  2:921518/1‑150 (MQ=255)
                                                                                                                             gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                        >  2:1149290/1‑150 (MQ=255)
                                                                                                                             gTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTc                        <  2:1238203/150‑1 (MQ=255)
                                                                                                                                    ttACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGc                 <  2:1087933/150‑1 (MQ=255)
                                                                                                                                     caCGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATATACTTGAAATGAAGAACATTAACAAAACCTTAGGCAGTGTGAAGGCGATTGATAACGTCTGCTTTCg                >  2:1499811/2‑150 (MQ=255)
                                                                                                                                       cGCCCCCGCTCCGCGCTGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGt              >  2:1236848/1‑150 (MQ=255)
                                                                                                                                         ccccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTg            <  2:1557828/150‑1 (MQ=255)
                                                                                                                                         ccccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTACGGCAGTGTGAAGGCGATTGATAACGTCTGCTGGCGGTTg            >  2:346143/1‑150 (MQ=255)
                                                                                                                                           ccAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGaa          <  2:940046/150‑1 (MQ=255)
                                                                                                                                               cgcgGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCt      <  1:360187/150‑1 (MQ=255)
                                                                                                                                                   gAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCg  <  2:1120437/150‑1 (MQ=255)
                                                                                                                                                   gAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAAATGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCg  <  1:224035/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAGAGAGCGAGCTGTAAGCGTTACGCCCCAGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATGCCTTATCTACTTGAAATGAAGAACATTACCAAAACCTTCGGCAGTGTGAAGGCGATTGATAACGTCTGCTTGCGGTTGAATGCTGGCG  >  NC_000913/3731995‑3732291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: