Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,736,225 T→G 7.1% intergenic (+68/+128) xylR → / ← bax xylose divergent operon transcriptional activator/putative glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,2250TG 7.1% 404.8 / 18.2 142intergenic (+68/+128)xylR/baxxylose divergent operon transcriptional activator/putative glucosaminidase
Reads supporting (aligned to +/- strand):  ref base T (61/69);  new base G (8/2);  total (71/71)
Fisher's exact test for biased strand distribution p-value = 5.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

Only 100 of 143 total aligned reads displayed.

ATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAA  >  NC_000913/3736076‑3736373
                                                                                                                                                     |                                                                                                                                                    
   tttCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTAt                                                                                                                                                   >  1:1485681/1‑150 (MQ=255)
     tCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGTGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                 <  1:310330/150‑1 (MQ=255)
       tACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGa                                                                                                                                               <  2:1614578/150‑1 (MQ=255)
        aCTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATAAGGCGTTCACGCCGCATCCGAc                                                                                                                                              <  2:34071/150‑1 (MQ=255)
           ctGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                                                                                                           >  2:1153385/1‑150 (MQ=255)
               ttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATTTAAATAGCGAGGTCATGATGTAGCGATGATGAGAATAGTCTGCGTCACATAAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCATATCCGACCTACCCg                                                                                                                                       >  2:51778/1‑150 (MQ=255)
               ttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCg                                                                                                                                       >  1:1263498/1‑150 (MQ=255)
                tttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGc                                                                                                                                      >  2:1508904/1‑150 (MQ=255)
                 ttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGAGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGcc                                                                                                                                     >  2:623575/1‑150 (MQ=255)
                   aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGCTGATGAGAATTGTCGGCGTCACATCAGGTACCGCTGCGTGGTTGTCGTATGCGGCGTGAACGCCTTATCCGACCTACCCGCAAg                                                                                                                                   >  1:848758/1‑150 (MQ=255)
                   aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCAAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAcc                                                                                                                                         <  1:1701162/144‑1 (MQ=255)
                   aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCAAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAcc                                                                                                                                         >  2:1701162/1‑144 (MQ=255)
                     aaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGc                                                                                                                                 >  2:1022373/1‑150 (MQ=255)
                        aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAATTAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                              >  2:244353/1‑150 (MQ=255)
                        aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                              >  1:1365985/1‑150 (MQ=255)
                        aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                              <  2:1263498/150‑1 (MQ=255)
                        aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                              <  2:1492991/150‑1 (MQ=255)
                         gCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTTGTTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACCCGCCAGGCATGa                                                                                                                             >  2:70677/1‑150 (MQ=255)
                         gCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGa                                                                                                                             <  1:1553738/150‑1 (MQ=255)
                         gCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGa                                                                                                                             <  2:1425557/150‑1 (MQ=255)
                          cATATGACACGACGCCAAAAGAGAATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGAGGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGAt                                                                                                                            <  1:1169740/150‑1 (MQ=255)
                           atatGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATa                                                                                                                           >  2:1695700/1‑150 (MQ=255)
                            tatGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaa                                                                                                                          >  2:643072/1‑150 (MQ=255)
                              tGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCAGGATaaaa                                                                                                                        <  1:152944/150‑1 (MQ=255)
                               gACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAAc                                                                                                                       >  1:146316/1‑150 (MQ=255)
                                        ccAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaaca                                                                                                              >  1:744049/1‑150 (MQ=255)
                                               agTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTc                                                                                                       >  2:678500/1‑150 (MQ=255)
                                               agTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTc                                                                                                       <  2:789878/150‑1 (MQ=255)
                                                ttagcgcgATGTAAATAGCGAGGTCATGTTGTAGCTATGTTGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGTTTGTCGGATGCGGCGTGAACGCCTTATACGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCa                                                                                                      <  1:331160/146‑1 (MQ=255)
                                                 tATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGGATGATAAAACGCACCAACAACGCTTCAg                                                                                                     <  1:1608660/150‑1 (MQ=255)
                                                  aTCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAgg                                                                                                    <  2:1545318/150‑1 (MQ=255)
                                                     gcgATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCATTATCCGACCTACCCGCCAGGAAAGATAAAACGCACCAACAACGCTTCAGGCTc                                                                                                 >  1:682273/1‑150 (MQ=255)
                                                       gATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGt                                                                                               >  1:672154/1‑150 (MQ=255)
                                                           tAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCCACAACGCTTCAGGCTCGTAGCt                                                                                           >  1:111222/1‑150 (MQ=255)
                                                            aaaTAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTc                                                                                          <  2:549499/150‑1 (MQ=255)
                                                            aaaTAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTc                                                                                          >  2:70239/1‑150 (MQ=255)
                                                               tAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAAc                                                                                       >  2:259378/1‑150 (MQ=255)
                                                                 gCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTg                                                                                     >  1:517329/1‑150 (MQ=255)
                                                                  cGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGc                                                                                    >  1:639764/1‑150 (MQ=255)
                                                                     ggTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTg                                                                                 <  2:1537035/150‑1 (MQ=255)
                                                                      gTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTga                                                                                >  1:1680808/1‑150 (MQ=255)
                                                                      gTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTga                                                                                >  1:1123187/1‑150 (MQ=255)
                                                                       tCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTgag                                                                               <  1:1244642/150‑1 (MQ=255)
                                                                            ttgtAGCGATGATGAGATTTGTCGGCGTCACATCAGGTTACGCTGCGTGGTTGTCGCATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCTACTGCCTGAGACaaa                                                                          <  1:85325/150‑1 (MQ=255)
                                                                             tgtAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAg                                                                         <  1:1153385/150‑1 (MQ=255)
                                                                             tgtAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAg                                                                         <  2:33565/150‑1 (MQ=255)
                                                                              gtAGCGATGATGAGTATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCTAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGt                                                                        <  2:1019751/150‑1 (MQ=255)
                                                                               tAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTa                                                                       <  2:83108/150‑1 (MQ=255)
                                                                                    atgatgAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAg                                                                  <  1:195773/150‑1 (MQ=255)
                                                                                     tgatgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                 >  2:1417451/1‑150 (MQ=255)
                                                                                     tgatgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                 <  2:544742/150‑1 (MQ=255)
                                                                                     tgatgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                 <  2:82458/150‑1 (MQ=255)
                                                                                     tgatgaGAATAGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGAATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                 <  2:1630270/150‑1 (MQ=255)
                                                                                       atgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCAGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCt                                                               >  2:1234351/1‑150 (MQ=255)
                                                                                        tgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCtt                                                              <  1:1028667/150‑1 (MQ=255)
                                                                                        tgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCtt                                                              <  1:1022373/150‑1 (MQ=255)
                                                                                         gagaATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTa                                                             <  2:561770/150‑1 (MQ=255)
                                                                                         gagaATTGTAGGCGTCACATCAGGTATCGATGCGTGGTTGGCGGATGCGTCGTGAACGCCTTATCCGACCTACCCTCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTa                                                             <  2:268871/150‑1 (MQ=255)
                                                                                           gaATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGATCGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATc                                                           >  1:1719527/1‑150 (MQ=255)
                                                                                              ttGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGa                                                        <  2:146316/150‑1 (MQ=255)
                                                                                               tGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGAc                                                       <  1:1437458/150‑1 (MQ=255)
                                                                                               tGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGAc                                                       <  1:678500/150‑1 (MQ=255)
                                                                                                   ggCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                   <  2:1132657/150‑1 (MQ=255)
                                                                                                    gCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACa                                                  <  2:659325/150‑1 (MQ=255)
                                                                                                       tCACATCAGGTAACGCTGCGTGGTTGTCGGCTGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAgg                                               >  2:1345874/1‑149 (MQ=255)
                                                                                                       tCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGt                                               >  2:1210713/1‑150 (MQ=255)
                                                                                                       tCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGATCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGt                                               >  2:870276/1‑150 (MQ=255)
                                                                                                          caTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCgg                                            <  1:1586115/150‑1 (MQ=255)
                                                                                                          caTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCgg                                            >  1:943300/1‑150 (MQ=255)
                                                                                                           aTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGa                                           <  2:517329/150‑1 (MQ=255)
                                                                                                             cAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATa                                         <  2:353193/150‑1 (MQ=255)
                                                                                                              aGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATaa                                        >  1:100144/1‑150 (MQ=255)
                                                                                                               ggTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAg                                       <  1:234453/150‑1 (MQ=255)
                                                                                                                gTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAGTCGGATAAgg                                      <  2:111222/150‑1 (MQ=255)
                                                                                                                 tAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGc                                     <  1:381328/150‑1 (MQ=255)
                                                                                                                 tAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAACAACAACGCATCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGc                                     >  2:65522/1‑150 (MQ=255)
                                                                                                                 tAACGCTGCGTGGTTGTCGGATCCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGc                                     <  2:942669/150‑1 (MQ=255)
                                                                                                                           tGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGaaa                            >  2:782346/1‑149 (MQ=255)
                                                                                                                           tGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGaaa                            <  1:782346/149‑1 (MQ=255)
                                                                                                                            ggTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATg                          >  2:1344133/1‑150 (MQ=255)
                                                                                                                             gTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGc                         <  2:1365985/150‑1 (MQ=255)
                                                                                                                              ttGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCa                        >  2:1128937/1‑150 (MQ=255)
                                                                                                                               ggTCGGATAAGGCGTACACGCCGCATCCGACCTACCCGCCAGCCATCATAAAACGCACCAACAACCCTTCAGGCACGAAGCTCAACTGCCAGAGACAAAGTAAAAAGCCTTATACGACTCACAAGTCGGATAATGCTCAAGGAAATGCaa                       >  2:750100/2‑150 (MQ=255)
                                                                                                                                gTCGGATAAGGCGTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAAt                      >  1:1224942/1‑150 (MQ=255)
                                                                                                                                  cGGATAAGGCGTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTa                    >  1:395579/1‑150 (MQ=255)
                                                                                                                                  cGGATAAGGCGTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCAGAGACAAAGTAAAACGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTa                    >  1:113113/1‑150 (MQ=255)
                                                                                                                                   ggataaggcgTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTaa                   >  2:743022/7‑149 (MQ=255)
                                                                                                                                   ggataaggcgTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTAc                   >  2:768050/7‑150 (MQ=255)
                                                                                                                                   ggataaggcgTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTAc                   >  2:1183837/7‑150 (MQ=255)
                                                                                                                                      tGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACata                <  2:49971/150‑1 (MQ=255)
                                                                                                                                       gcggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCTCCCACACCGCTTCAGGCTCGTAGCTCCACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCTAGGAAATGCAATCCCatat               >  1:224651/1‑150 (MQ=255)
                                                                                                                                        cggcgTGAACGCCTTATCCGACCTACCCGCCATGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg              <  1:918796/150‑1 (MQ=255)
                                                                                                                                        cggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg              >  1:1670464/1‑150 (MQ=255)
                                                                                                                                        cggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAACAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg              <  1:1344133/150‑1 (MQ=255)
                                                                                                                                         ggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATgc             >  1:1382837/1‑150 (MQ=255)
                                                                                                                                            gTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGcgcc          <  2:1123187/150‑1 (MQ=255)
                                                                                                                                             tGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGcgccg         >  1:328586/1‑150 (MQ=255)
                                                                                                                                                 cGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTTTCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAt     <  1:870276/150‑1 (MQ=255)
                                                                                                                                                 cGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAt     <  2:21914/150‑1 (MQ=255)
                                                                                                                                                    cTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAAAGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAACGAAATGCAATTACATATGCGCCGAGATTaa  >  2:562710/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATATTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAA  >  NC_000913/3736076‑3736373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: