Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,736,226 T→C 7.0% intergenic (+69/+127) xylR → / ← bax xylose divergent operon transcriptional activator/putative glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,736,2260TC 7.0% 409.7 / 16.9 142intergenic (+69/+127)xylR/baxxylose divergent operon transcriptional activator/putative glucosaminidase
Reads supporting (aligned to +/- strand):  ref base T (63/69);  new base C (8/2);  total (71/71)
Fisher's exact test for biased strand distribution p-value = 9.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01

Only 100 of 144 total aligned reads displayed.

TTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACC  >  NC_000913/3736079‑3736375
                                                                                                                                                   |                                                                                                                                                     
 ttCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                    >  2:655833/1‑150 (MQ=255)
  tCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGTGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                   <  1:310330/150‑1 (MQ=255)
    tACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGa                                                                                                                                                 <  2:1614578/150‑1 (MQ=255)
     aCTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATAAGGCGTTCACGCCGCATCCGAc                                                                                                                                                <  2:34071/150‑1 (MQ=255)
          gTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAcc                                                                                                                                           >  1:21914/1‑150 (MQ=255)
            ttttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCg                                                                                                                                         >  1:1263498/1‑150 (MQ=255)
             tttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGc                                                                                                                                        >  2:1508904/1‑150 (MQ=255)
              ttAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGAGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGcc                                                                                                                                       >  2:623575/1‑150 (MQ=255)
                aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGCTGATGAGAATTGTCGGCGTCACATCAGGTACCGCTGCGTGGTTGTCGTATGCGGCGTGAACGCCTTATCCGACCTACCCGCAAg                                                                                                                                     >  1:848758/1‑150 (MQ=255)
                aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGATAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAg                                                                                                                                     >  2:793167/1‑150 (MQ=255)
                aaaaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCAAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAcc                                                                                                                                           >  2:1701162/1‑144 (MQ=255)
                  aaaaGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGc                                                                                                                                   >  2:1022373/1‑150 (MQ=255)
                     aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAATTAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                                >  2:244353/1‑150 (MQ=255)
                     aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                                >  1:1365985/1‑150 (MQ=255)
                     aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                                <  2:1263498/150‑1 (MQ=255)
                     aGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATg                                                                                                                                <  2:1492991/150‑1 (MQ=255)
                      gCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGa                                                                                                                               <  2:1425557/150‑1 (MQ=255)
                      gCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGa                                                                                                                               <  1:1553738/150‑1 (MQ=255)
                       cATATGACACGACGCCAAAAGAGAATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGAGGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGAt                                                                                                                              <  1:1169740/150‑1 (MQ=255)
                        atatGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATa                                                                                                                             >  2:1695700/1‑150 (MQ=255)
                         tatGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaa                                                                                                                            >  2:643072/1‑150 (MQ=255)
                           tGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATaaaa                                                                                                                          <  1:171770/150‑1 (MQ=255)
                           tGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCAGGATaaaa                                                                                                                          <  1:152944/150‑1 (MQ=255)
                            gACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAAc                                                                                                                         >  1:146316/1‑150 (MQ=255)
                             acacGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACg                                                                                                                        >  2:52499/1‑150 (MQ=255)
                                cgacgCCAAAAGAGTATCGCGATGTTAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAc                                                                                                                     <  2:557076/150‑1 (MQ=255)
                                cgacgCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGTCCTACCCGCCAGGCATGATAAAACGCAc                                                                                                                     <  1:658876/150‑1 (MQ=255)
                                     ccAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACcaaca                                                                                                                >  1:744049/1‑150 (MQ=255)
                                              tATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGGATGATAAAACGCACCAACAACGCTTCAg                                                                                                       <  1:1608660/150‑1 (MQ=255)
                                                  gcgATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCATTATCCGACCTACCCGCCAGGAAAGATAAAACGCACCAACAACGCTTCAGGCTc                                                                                                   >  1:682273/1‑150 (MQ=255)
                                                        tAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCCACAACGCTTCAGGCTCGTAGCt                                                                                             >  1:111222/1‑150 (MQ=255)
                                                         aaaTAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTc                                                                                            >  2:70239/1‑150 (MQ=255)
                                                              gCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTg                                                                                       >  1:517329/1‑150 (MQ=255)
                                                               cGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGc                                                                                      >  1:639764/1‑150 (MQ=255)
                                                                gAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGcc                                                                                     >  2:26389/1‑150 (MQ=255)
                                                                  ggTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTg                                                                                   <  2:1537035/150‑1 (MQ=255)
                                                                   gTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTga                                                                                  >  1:1680808/1‑150 (MQ=255)
                                                                   gTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTga                                                                                  >  1:1123187/1‑150 (MQ=255)
                                                                    tCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTgag                                                                                 <  1:1244642/150‑1 (MQ=255)
                                                                         ttgtAGCGATGATGAGATTTGTCGGCGTCACATCAGGTTACGCTGCGTGGTTGTCGCATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCTACTGCCTGAGACaaa                                                                            <  1:85325/150‑1 (MQ=255)
                                                                          tgtAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAg                                                                           <  1:1153385/150‑1 (MQ=255)
                                                                           gtAGCGATGATGAGTATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCTAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGt                                                                          <  2:1019751/150‑1 (MQ=255)
                                                                                  tgatgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                   >  2:1417451/1‑150 (MQ=255)
                                                                                  tgatgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                   <  2:82458/150‑1 (MQ=255)
                                                                                  tgatgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGc                                                                   <  2:544742/150‑1 (MQ=255)
                                                                                    atgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCAGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCt                                                                 >  2:1234351/1‑150 (MQ=255)
                                                                                     tgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCtt                                                                >  1:149815/1‑150 (MQ=255)
                                                                                     tgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCtt                                                                <  1:1028667/150‑1 (MQ=255)
                                                                                     tgaGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCtt                                                                <  1:1022373/150‑1 (MQ=255)
                                                                                      gagaATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTa                                                               <  2:561770/150‑1 (MQ=255)
                                                                                      gagaATTGTAGGCGTCACATCAGGTATCGATGCGTGGTTGGCGGATGCGTCGTGAACGCCTTATCCGACCTACCCTCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTa                                                               <  2:268871/150‑1 (MQ=255)
                                                                                        gaATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGATCGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATc                                                             >  1:1719527/1‑150 (MQ=255)
                                                                                           ttGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGa                                                          <  2:146316/150‑1 (MQ=255)
                                                                                            tGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGAc                                                         <  1:678500/150‑1 (MQ=255)
                                                                                            tGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGAc                                                         <  1:1437458/150‑1 (MQ=255)
                                                                                                ggCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGAc                                                     <  2:1132657/150‑1 (MQ=255)
                                                                                                 gCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACa                                                    <  2:659325/150‑1 (MQ=255)
                                                                                                    tCACATCAGGTAACGCTGCGTGGTTGTCGGCTGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAgg                                                 >  2:1345874/1‑149 (MQ=255)
                                                                                                    tCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGt                                                 >  2:1210713/1‑150 (MQ=255)
                                                                                                    tCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGAACTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGATCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGt                                                 >  2:870276/1‑150 (MQ=255)
                                                                                                     cacaTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCTGGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAACCGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTc                                                <  2:866427/150‑1 (MQ=255)
                                                                                                       caTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCgg                                              <  1:1586115/150‑1 (MQ=255)
                                                                                                       caTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCgg                                              >  1:943300/1‑150 (MQ=255)
                                                                                                       caTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCgg                                              <  2:614354/150‑1 (MQ=255)
                                                                                                        aTCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGa                                             <  2:517329/150‑1 (MQ=255)
                                                                                                           aGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATaa                                          >  1:100144/1‑150 (MQ=255)
                                                                                                           aGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATaa                                          >  2:1410988/1‑150 (MQ=255)
                                                                                                            ggTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAg                                         <  1:234453/150‑1 (MQ=255)
                                                                                                             gTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCTGACTGACAAGTCGGATAAgg                                        <  2:1680808/150‑1 (MQ=255)
                                                                                                             gTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTTGTCAGTCGGATAAgg                                        <  2:111222/150‑1 (MQ=255)
                                                                                                              tAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGc                                       <  1:381328/150‑1 (MQ=255)
                                                                                                              tAACGCTGCGTGGTTGTCGGATCCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGc                                       <  2:942669/150‑1 (MQ=255)
                                                                                                                   cTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAg                                  <  2:1577626/150‑1 (MQ=255)
                                                                                                                        tGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGaaa                              <  1:782346/149‑1 (MQ=255)
                                                                                                                        tGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGaaa                              >  2:782346/1‑149 (MQ=255)
                                                                                                                         ggTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATg                            <  2:773941/150‑1 (MQ=255)
                                                                                                                         ggTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATg                            >  2:1344133/1‑150 (MQ=255)
                                                                                                                           ttGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCa                          >  2:46812/1‑150 (MQ=255)
                                                                                                                           ttGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCa                          >  2:1128937/1‑150 (MQ=255)
                                                                                                                             gTCGGATAAGGCGTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAAt                        >  1:1224942/1‑150 (MQ=255)
                                                                                                                              gcGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAAtt                       <  2:682273/149‑1 (MQ=255)
                                                                                                                               cGGATAAGGCGTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTa                      >  1:395579/1‑150 (MQ=255)
                                                                                                                               cGGATAAGGCGTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCAGAGACAAAGTAAAACGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTa                      >  1:113113/1‑150 (MQ=255)
                                                                                                                                ggataaggcgTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTaa                     >  2:743022/7‑149 (MQ=255)
                                                                                                                                ggataaggcgTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTAc                     >  2:768050/7‑150 (MQ=255)
                                                                                                                                ggataaggcgTTCACGCCGCATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTAc                     >  2:1183837/7‑150 (MQ=255)
                                                                                                                                 gATGCGGCGTGAACGCCTTATCCTACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACa                    <  2:1153943/150‑1 (MQ=255)
                                                                                                                                   tGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACata                  <  2:49971/150‑1 (MQ=255)
                                                                                                                                    gcggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCTCCCACACCGCTTCAGGCTCGTAGCTCCACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCTAGGAAATGCAATCCCatat                 >  1:224651/1‑150 (MQ=255)
                                                                                                                                     cggcgTGAACGCCTTATCCGACCTACCCGCCATGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg                <  1:918796/150‑1 (MQ=255)
                                                                                                                                     cggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg                >  1:1670464/1‑150 (MQ=255)
                                                                                                                                     cggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCAACAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATg                <  1:1344133/150‑1 (MQ=255)
                                                                                                                                      ggcgTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATgc               >  1:1382837/1‑150 (MQ=255)
                                                                                                                                         gTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATa                                           <  2:724110/119‑1 (MQ=255)
                                                                                                                                         gTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGcgcc            <  2:1123187/150‑1 (MQ=255)
                                                                                                                                          tGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGcgccg           >  1:328586/1‑150 (MQ=255)
                                                                                                                                             aCGCCATATCCGACCTACCCGCCAGGCAAGATAAAACGCACCAACACCGCTTCAGTCTCGTAGCTCAACTGCCTGAGACAAAGGAAAAAGCCTTATCCGACTGACAAGACGCATAAGGCTCAAGGAAATGCAATTACAAATGCGCCGCGa        >  2:340394/1‑150 (MQ=255)
                                                                                                                                              cGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTTTCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAt       <  1:870276/150‑1 (MQ=255)
                                                                                                                                              cGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGAt       <  2:21914/150‑1 (MQ=255)
                                                                                                                                                   tATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAAcc  <  2:943300/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTTCTACTCTGTTTTTAAAAAAGCATATGACACGACGCCAAAAGAGTATCGCGATGTAAATAGCGAGGTCATGTTGTAGCGATGATGAGAATTGTCGGCGTCACATCAGGTAACGCTGCGTGGTTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACCCGCCAGGCATGATAAAACGCACCAACAACGCTTCAGGCTCGTAGCTCAACTGCCTGAGACAAAGTAAAAAGCCTTATCCGACTGACAAGTCGGATAAGGCTCAAGGAAATGCAATTACATATGCGCCGCGATTAACC  >  NC_000913/3736079‑3736375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: