Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,740,791 G→C 5.3% G363R (GGC→CGC)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,740,7910GC 5.3% 353.5 / 7.3 110G363R (GGC→CGC) avtAvaline‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
Reads supporting (aligned to +/- strand):  ref base G (41/61);  new base C (4/2);  total (46/64)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-01

Only 100 of 115 total aligned reads displayed.

GTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGC  >  NC_000913/3740644‑3740939
                                                                                                                                                   |                                                                                                                                                    
gtTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcggc                                                                                                                                                    <  2:879167/150‑1 (MQ=255)
gtTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcggc                                                                                                                                                    >  2:1190422/1‑150 (MQ=255)
gtTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACgcggc                                                                                                                                                    <  1:1190422/150‑1 (MQ=255)
    aGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCGTCTGGCTATGGTTTAAGGATTTGCGCATTAGGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGAGGCGTGc                                                                                                                                                >  2:25712/1‑150 (MQ=255)
        aaCTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGAt                                                                                                                                            <  1:1214659/150‑1 (MQ=255)
           tATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCCGCGTGCtttcagg                                                                                                                                         <  1:19412/150‑7 (MQ=255)
            aTCGCCATCATTCTCCGCTATTTTCCGGTATATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTg                                                                                                                                        <  2:1148730/150‑1 (MQ=255)
            aTCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTg                                                                                                                                        >  2:1264425/1‑150 (MQ=255)
              cGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcc                                                                                                                                      >  2:580148/1‑150 (MQ=255)
              cGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGcc                                                                                                                                      >  1:119331/1‑150 (MQ=255)
               gctatcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                     >  2:1164826/4‑150 (MQ=255)
               gCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCg                                                                                                                                     >  2:1644561/1‑150 (MQ=255)
                ccATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgg                                                                                                                                    <  1:963666/150‑1 (MQ=255)
                 catcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCggg                                                                                                                                   >  1:372994/1‑150 (MQ=255)
                  atcatTCGCCTCTCTTTACCGGAAAATCGGTGCCGTATTCATACACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATATGCCCATTACGACCAAGCAGCTCTATGAGCGCCTGAAAGCTCGCGGCGTGCTGATGGTGCCgggg                                                                                                                                  <  2:1055740/150‑1 (MQ=255)
                  atcatTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCgggg                                                                                                                                  >  2:773907/1‑150 (MQ=255)
                    catTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGca                                                                                                                                <  1:432403/150‑1 (MQ=255)
                        cgccgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAc                                                                                                                            <  1:709182/150‑1 (MQ=255)
                         gccgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAct                                                                                                                           <  1:106296/150‑1 (MQ=255)
                         gccgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAct                                                                                                                           <  1:988797/150‑1 (MQ=255)
                         gccgcTATTTACCGGAAAATCGCTGCCGGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTAGGGTTTAAGGATTTGCCCATTACGACCACGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAAct                                                                                                                           <  1:84937/150‑1 (MQ=255)
                           cgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtc                                                                                                                         <  1:533243/150‑1 (MQ=255)
                           cgcTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtc                                                                                                                         <  2:1578780/150‑1 (MQ=255)
                           cgcTATTTACCGGAAAATCGCTGCCTGATTCAAAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCCCGGcaccatcag                                                                                                                         <  1:1627161/150‑7 (MQ=255)
                           cgcTATTTACCGGAAAATCGCTGCCTGATGCATAACCCGGACCGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCACCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAActtc                                                                                                                         <  2:155973/150‑1 (MQ=255)
                              tATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAATCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACttcttc                                                                                                                      <  1:1310269/150‑1 (MQ=255)
                              tATTTACACGAAAATCGCTGCCTGATTCATAAACCGGACGGAGCCATATTCCTCTGGCTATGGTTTAAGGCTTTGCCCATTACCACCAAGCAGCACTATCAGCGCATGAAAGCACGCTGCGTGCTGATGGTGCCGGGGCACTACttcttc                                                                                                                      <  1:729338/150‑1 (MQ=255)
                                tttACCGGAACATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCGCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCTCCTGATAGCACGAGGCGTGCTGATTGTGCCGGGTCACAACTTCTTccc                                                                                                                    <  1:1494264/150‑1 (MQ=255)
                                  tACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAg                                                                                                                  <  2:461485/150‑1 (MQ=255)
                                     cGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGc                                                                                                               >  2:925790/1‑150 (MQ=255)
                                      ggAAAATCGCTGCGTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCAGAAAGCACGCGGCGTGATGATGGTGCCGGGGCACAACTTCTTCCCAGGGCt                                                                                                              >  1:624913/1‑150 (MQ=255)
                                      ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCt                                                                                                              >  1:569598/1‑150 (MQ=255)
                                      ggAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTg                                                                                                             <  1:956102/150‑1 (MQ=255)
                                        aaaaTCGCTTCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCGCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTgg                                                                                                            <  1:1090167/150‑1 (MQ=255)
                                           aTCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATa                                                                                                         <  1:1164826/150‑1 (MQ=255)
                                              gCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAc                                                                                                      <  2:259457/150‑1 (MQ=255)
                                              gCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAc                                                                                                      >  1:736375/1‑150 (MQ=255)
                                               cTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAAcc                                                                                                     >  1:981244/1‑150 (MQ=255)
                                                tGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAACCCg                                                                                                    >  1:1310070/1‑150 (MQ=255)
                                                   cTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTgg                                                                                                 >  1:1676233/1‑150 (MQ=255)
                                                    tGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGc                                                                                                <  2:1951/150‑1 (MQ=255)
                                                    tGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGc                                                                                                >  1:1258888/1‑150 (MQ=255)
                                                        tCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCa                                                                                            <  2:1188985/150‑1 (MQ=255)
                                                        tCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCa                                                                                            <  2:119331/150‑1 (MQ=255)
                                                        tCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCa                                                                                            <  1:682929/150‑1 (MQ=255)
                                                         cATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTATGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGAGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCAt                                                                                           >  1:162370/1‑150 (MQ=255)
                                                          aTAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATa                                                                                          >  1:1648559/1‑150 (MQ=255)
                                                          aTAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCACCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATa                                                                                          >  2:1203571/1‑150 (MQ=255)
                                                              aCCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCa                                                                                      >  1:1319910/1‑150 (MQ=255)
                                                                cGGAAGGAGCCATTTTCCTCTGGCTATGGTGTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATc                                                                                    <  1:382176/150‑1 (MQ=255)
                                                                    aGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCCCGCGGCGTGCGGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATg                                                                                <  1:877660/150‑1 (MQ=255)
                                                                        gCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCAATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCTGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATg                                                                            >  2:231388/1‑150 (MQ=255)
                                                                           aTTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATgcgc                                                                         <  1:364779/150‑1 (MQ=255)
                                                                            ttttCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCa                                                                        >  2:285341/1‑150 (MQ=255)
                                                                             tttCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCAt                                                                       <  2:1648559/150‑1 (MQ=255)
                                                                                ccTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACACCTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGaa                                                                    >  2:1234225/1‑150 (MQ=255)
                                                                                 ctctGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAAc                                                                   >  1:1093504/1‑150 (MQ=255)
                                                                                   ctGGCTATGGTTTAAGGATTTGCCCATTATGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGAGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTa                                                                 <  2:162370/150‑1 (MQ=255)
                                                                                     ggCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACg                                                               <  2:5883/150‑1 (MQ=255)
                                                                                       cTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTa                                                             >  2:703932/1‑150 (MQ=255)
                                                                                         aTGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGAGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTAcc                                                           <  1:773907/150‑1 (MQ=255)
                                                                                          tGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCa                                                          <  1:299043/150‑1 (MQ=255)
                                                                                          tGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGGGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCa                                                          <  1:303568/150‑1 (MQ=255)
                                                                                             tttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCagag                                                       <  2:733774/150‑1 (MQ=255)
                                                                                             tttAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCagag                                                       <  2:736375/150‑1 (MQ=255)
                                                                                               tAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGcc                                                     <  2:499648/150‑1 (MQ=255)
                                                                                               tAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGcc                                                     <  2:1276954/150‑1 (MQ=255)
                                                                                                aaGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCg                                                    >  2:1016657/1‑150 (MQ=255)
                                                                                                 aGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCgg                                                   <  1:1395323/150‑1 (MQ=255)
                                                                                                  ggATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGga                                                  <  2:569598/150‑1 (MQ=255)
                                                                                                           cATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGa                                         <  2:1319910/150‑1 (MQ=255)
                                                                                                           cATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGa                                         >  2:1473975/1‑150 (MQ=255)
                                                                                                               aCGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTgcggcg                                     >  1:501532/1‑150 (MQ=255)
                                                                                                                 caCCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCggg                                   <  2:624913/149‑1 (MQ=255)
                                                                                                                  aCCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCgggg                                  <  1:1264425/150‑1 (MQ=255)
                                                                                                                    cAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGAGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGt                                <  1:285341/150‑1 (MQ=255)
                                                                                                                      agcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGa                              <  1:1644561/150‑1 (MQ=255)
                                                                                                                       gcagcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGaa                             <  1:1132720/150‑1 (MQ=255)
                                                                                                                        cagcTCTATCAGCGCCTGAAAGCCCGCGGCGTGCTGATGGTTCCGGGGCACAACTTCTTCCCACGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAg                            <  1:514468/150‑1 (MQ=255)
                                                                                                                         agcTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGa                           >  2:1245774/1‑150 (MQ=255)
                                                                                                                          gcTCTATCAGCGCCTGAAAGCACGAGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGAt                          <  1:1200035/150‑1 (MQ=255)
                                                                                                                             ctATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCt                       >  2:1569154/1‑150 (MQ=255)
                                                                                                                                 ccgcgcCTGAAAGCACGCGGCGAGCAGATGGTGCCGGGGCACAACTTCATCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCAAGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCg                   <  1:1606281/148‑1 (MQ=255)
                                                                                                                                 cAGCGCCTGACAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTACCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCg                   <  1:1105313/150‑1 (MQ=255)
                                                                                                                                 cAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCg                   <  2:628694/150‑1 (MQ=255)
                                                                                                                                  aGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCgg                  >  2:51049/1‑150 (MQ=255)
                                                                                                                                  aGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCgg                  <  1:507421/150‑1 (MQ=255)
                                                                                                                                  aGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCgg                  >  2:1471039/1‑150 (MQ=255)
                                                                                                                                    cgcCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGaa                >  1:1477470/1‑150 (MQ=255)
                                                                                                                                      ccTGAAAGCACGCGGCGTGCTGATGGTGTCGGGGCACCACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAagg              >  1:241360/1‑149 (MQ=255)
                                                                                                                                      accatcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAaga              >  2:266936/7‑150 (MQ=255)
                                                                                                                                       ccatcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAagag             >  1:183224/6‑150 (MQ=255)
                                                                                                                                        catcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATCCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAgg                                       >  1:1200848/5‑123 (MQ=255)
                                                                                                                                        catcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCACAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAgg                                       <  2:1200848/119‑1 (MQ=255)
                                                                                                                                         atcaGCACGCCGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGAt           >  1:1631046/4‑150 (MQ=255)
                                                                                                                                          aaaGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATa          >  1:1128649/1‑150 (MQ=255)
                                                                                                                                             gCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGaa       >  1:1479760/1‑150 (MQ=255)
                                                                                                                                               aCGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAag     <  1:1016657/150‑1 (MQ=255)
                                                                                                                                                cgcgGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAaga    <  1:703932/150‑1 (MQ=255)
                                                                                                                                                  cggcgTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGc  >  1:1124650/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTTCAGGAAACTATCGCCATCATTCGCCGCTATTTACCGGAAAATCGCTGCCTGATTCATAAACCGGAAGGAGCCATTTTCCTCTGGCTATGGTTTAAGGATTTGCCCATTACGACCAAGCAGCTCTATCAGCGCCTGAAAGCACGCGGCGTGCTGATGGTGCCGGGGCACAACTTCTTCCCAGGGCTGGATAAACCGTGGCCGCATACGCATCAATGTATGCGCATGAACTACGTACCAGAGCCGGAGAAAATTGAGGCGGGGGTGAAGATTCTGGCGGAAGAGATAGAAAGAGC  >  NC_000913/3740644‑3740939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: