Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,756,565 C→T 5.1% intergenic (‑54/+111) aldB ← / ← yiaY aldehyde dehydrogenase B/L‑threonine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,756,5650CT 5.1% 440.9 / 5.6 135intergenic (‑54/+111)aldB/yiaYaldehyde dehydrogenase B/L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (68/60);  new base T (4/3);  total (72/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTG  >  NC_000913/3756418‑3756714
                                                                                                                                                   |                                                                                                                                                     
 gccgccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCtta                                                                                                                                                    >  1:1589172/1‑149 (MQ=255)
  ccgccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCTTACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGTTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCg                                                                                                                                                   <  1:1216534/150‑1 (MQ=255)
  ccgccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGGCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGCCGTGACTGGCGAAATCTTCg                                                                                                                                                   >  1:1564783/1‑150 (MQ=255)
   cgccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGc                                                                                                                                                  >  1:225050/1‑150 (MQ=255)
   cgccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGc                                                                                                                                                  <  2:1295009/150‑1 (MQ=255)
    gccAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGcc                                                                                                                                                 >  2:32566/1‑150 (MQ=255)
    gccAATAAAGTTGTCATAGCGGGCATTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGcc                                                                                                                                                 <  1:1433492/150‑1 (MQ=255)
     ccAATAAAGTTGTCCTAGCGGGCTTTTACCTTGCGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCa                                                                                                                                                <  2:1610795/150‑1 (MQ=255)
      cAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATATGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTTGCGAAATCTTCGCCAg                                                                                                                                               >  1:951785/1‑150 (MQ=255)
       aaTAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGt                                                                                                                                              <  2:1648111/150‑1 (MQ=255)
          aaaGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTcac                                                                                                                                           <  1:954305/150‑3 (MQ=255)
           acgTTGGCATAGCGGGCTTTTATCTTGAGGGGGAATCCATACTCGCCGGGGTTAATCTGTGCTGAAGGCGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGTGGTATAACAAGGGTAGACGTGACTGGCGAAGATTTCGCCAGTcacg                                                                                                                                          <  1:244120/148‑4 (MQ=255)
               tGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACa                                                                                                                                      <  1:1380366/150‑1 (MQ=255)
                      gCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTCGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTAc                                                                                                                               <  2:1301436/150‑1 (MQ=255)
                      gCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTAc                                                                                                                               <  1:942869/150‑1 (MQ=255)
                      gCGGGCGTTTACCTTGAGGGGGAAACCATACTCGCCGGGCTTACTCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTAc                                                                                                                               <  1:629685/150‑1 (MQ=255)
                         ggCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGAc                                                                                                                            <  2:22945/150‑1 (MQ=255)
                                 aCTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGATGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCa                                                                                                                    <  1:1073120/150‑1 (MQ=255)
                                 aCTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGATAGCTATCa                                                                                                                    >  1:1135683/1‑150 (MQ=255)
                                  cTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCAc                                                                                                                   >  1:1204257/1‑150 (MQ=255)
                                    tGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGa                                                                                                                 >  2:1683734/1‑150 (MQ=255)
                                      aGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAAt                                                                                                               >  1:769707/1‑150 (MQ=255)
                                       gggggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAAtt                                                                                                              >  2:398291/1‑150 (MQ=255)
                                        ggggAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAAttt                                                                                                             >  1:1153676/1‑150 (MQ=255)
                                            aaaCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACgg                                                                                                         <  1:446134/150‑1 (MQ=255)
                                             aaCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACggg                                                                                                        <  1:678110/150‑1 (MQ=255)
                                             aaCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAAGCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACggg                                                                                                        <  1:1627881/150‑1 (MQ=255)
                                              aCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGc                                                                                                       >  2:1383923/1‑150 (MQ=255)
                                              aCCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGc                                                                                                       <  2:1647190/150‑1 (MQ=255)
                                               ccATACTCGCCGGGCTAAATATGTGCTGAAGGGCGATTATTCGTCATGATCGACTCCTTGCGGTGTGAGGTATAACAAGGTTAGACGTGACTGGCTAAAACTTCGCCAGTCGGAAACAGCTTTACGACAGCTATCACGAAATTACTGGCa                                                                                                      >  1:137011/1‑150 (MQ=255)
                                                cATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCaa                                                                                                     >  2:823501/1‑150 (MQ=255)
                                                  tACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAgg                                                                                                   >  1:1669577/1‑150 (MQ=255)
                                                   aCTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAggg                                                                                                  <  2:677811/150‑1 (MQ=255)
                                                   aCTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCAGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAggg                                                                                                  >  2:523149/1‑150 (MQ=255)
                                                    cTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGt                                                                                                 >  2:466222/1‑150 (MQ=255)
                                                       gCCGGGCTTAATCTGTGCTGAAGGGGCATTATTGGTCATGATCGTCTCATTGCGGTGAGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGtaaa                                                                                              >  2:207125/1‑147 (MQ=255)
                                                         cGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACag                                                                                            >  1:697873/1‑150 (MQ=255)
                                                            gCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAgaga                                                                                         >  1:210714/1‑150 (MQ=255)
                                                                  tCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTg                                                                                   <  1:912400/150‑1 (MQ=255)
                                                                   cTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGa                                                                                  >  1:748205/1‑150 (MQ=255)
                                                                        cTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAg                                                                             <  1:668491/150‑1 (MQ=255)
                                                                         tGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTAGGTAACAGCGTTACGACAGCTATCACGAATTTCCGGGCAAGGGTTACCGAGCTAGTTGAAAAAGc                                                                            >  1:1525347/1‑150 (MQ=255)
                                                                          tatgggggATTATTGGTCATGATCGTGTCCTTGCGGTGTGATGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACATCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                                                                           <  2:1589172/147‑1 (MQ=255)
                                                                          gAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCg                                                                           <  2:1472195/150‑1 (MQ=255)
                                                                           aaGGGGGATTATTGTTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGACAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCgg                                                                          <  1:1277690/150‑1 (MQ=255)
                                                                           aaGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTATGGGCAAGGGTTACAGAGATAGTTGAAAAAGCgg                                                                          <  1:1110290/150‑1 (MQ=255)
                                                                           aaGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCgg                                                                          <  1:1005160/150‑1 (MQ=255)
                                                                            aGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGc                                                                         <  1:823501/150‑1 (MQ=255)
                                                                            aGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGc                                                                         >  2:503571/1‑150 (MQ=255)
                                                                             gggggATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACATAGATAGTTGAAAAAGCGGCt                                                                        >  2:1059028/1‑150 (MQ=255)
                                                                                   ttattGGTCATGATAGTCTCCTTGCGGTGTGAGGTATAACAAGGGTTGACGGGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACTAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAt                                                                  <  2:1500961/150‑1 (MQ=255)
                                                                                        ggTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGcc                                                             <  2:1536037/150‑1 (MQ=255)
                                                                                        ggTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGcc                                                             <  1:466222/150‑1 (MQ=255)
                                                                                        ggTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGcc                                                             <  1:398291/150‑1 (MQ=255)
                                                                                        ggTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAATTGcc                                                             >  1:852334/1‑150 (MQ=255)
                                                                                          tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAg                                                           >  2:943575/1‑150 (MQ=255)
                                                                                          tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAg                                                           <  2:1153676/150‑1 (MQ=255)
                                                                                          tCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTAGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTTCCAg                                                           >  1:1273480/1‑150 (MQ=255)
                                                                                           cATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGc                                                          >  2:1208023/1‑150 (MQ=255)
                                                                                           cATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGc                                                          <  1:1403290/150‑1 (MQ=255)
                                                                                           cATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGc                                                          >  1:344143/1‑150 (MQ=255)
                                                                                             tGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCg                                                        >  2:691556/1‑150 (MQ=255)
                                                                                                   tctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTgg                                                  >  1:817583/1‑150 (MQ=255)
                                                                                                   tctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTgg                                                  >  1:550786/1‑150 (MQ=255)
                                                                                                    ctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAg                                                           >  1:1569863/1‑140 (MQ=255)
                                                                                                    ctcCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAg                                                           <  2:1569863/140‑1 (MQ=255)
                                                                                                      ccTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGaaa                                               >  2:1700640/1‑150 (MQ=255)
                                                                                                        ttGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAatg                                             <  1:1433514/150‑1 (MQ=255)
                                                                                                         tGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAatga                                            >  1:1158784/1‑150 (MQ=255)
                                                                                                               gtgAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTa                                      >  2:1388953/1‑150 (MQ=255)
                                                                                                                 gAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTtgtg                                                   >  1:938157/1‑135 (MQ=255)
                                                                                                                 gAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTtgtg                                                   <  2:938157/135‑1 (MQ=255)
                                                                                                                  aGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACAt                                   >  2:638517/1‑150 (MQ=255)
                                                                                                                   ggTATAAAAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGGTTTACGACCGCTATCACGAATTTACGGGCAAGGGTTACAGAGATGGTTGATAAAGCGGCTACCAATTTGGCAGCAGTTGTGGAAATGATGATTACATc                                  >  2:131812/1‑150 (MQ=255)
                                                                                                                    gTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCg                                 >  2:899887/1‑150 (MQ=255)
                                                                                                                    gTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATAACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGACAGCCGTTTTGGAAATGATGATTACAtac                                 >  2:1446863/1‑148 (MQ=255)
                                                                                                                      ataACAAGGGTAGACGTGACTGGCGAAATCTTAGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGTCAGCCGTTGTGGAAATGATGATTACATCGCt                               >  2:663710/1‑150 (MQ=255)
                                                                                                                         aCAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAAATTGCCAGCCGTTGTGGAAATGATTAATACATCGCTgcg                            >  1:633777/1‑150 (MQ=255)
                                                                                                                          cAAGGGTAGCCGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTCCGACAGCTATCACGAATTTACGGTCAAGGGTTACAGAGATTGTTTAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTgcgc                           >  1:65970/1‑150 (MQ=255)
                                                                                                                          cAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAatgatg                                          <  1:111970/135‑1 (MQ=255)
                                                                                                                          cAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAatgatg                                          >  2:111970/1‑135 (MQ=255)
                                                                                                                          cAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTgcgc                           <  1:503571/150‑1 (MQ=255)
                                                                                                                          cAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTgcgc                           >  2:1696139/1‑150 (MQ=255)
                                                                                                                          cAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTgcgc                           >  1:759167/1‑150 (MQ=255)
                                                                                                                              ggTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATa                       <  2:210714/150‑1 (MQ=255)
                                                                                                                               gTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATaa                      >  1:41606/1‑150 (MQ=255)
                                                                                                                                 gttaccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAAt                    >  1:693471/7‑150 (MQ=255)
                                                                                                                                    cGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGc                 <  2:1204257/150‑1 (MQ=255)
                                                                                                                                     ccgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGcc                >  1:1470819/3‑150 (MQ=255)
                                                                                                                                      tGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCca               >  1:1199753/1‑150 (MQ=255)
                                                                                                                                      cgACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCca               >  2:751411/2‑150 (MQ=255)
                                                                                                                                       gACTGGCGAAGATTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCcac              >  2:691352/1‑150 (MQ=255)
                                                                                                                                       gACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCcac              >  1:1055014/1‑150 (MQ=255)
                                                                                                                                             cGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATttc        >  2:664563/1‑150 (MQ=255)
                                                                                                                                              gAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATttct       >  2:393451/1‑150 (MQ=255)
                                                                                                                                                aaTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATttcttc     >  2:282473/1‑150 (MQ=255)
                                                                                                                                                 aTCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCg    <  2:655365/150‑1 (MQ=255)
                                                                                                                                                   cTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTg  <  1:512021/150‑1 (MQ=255)
                                                                                                                                                   cTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTg  <  2:1461699/150‑1 (MQ=255)
                                                                                                                                                   cTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAAGCGCAACAATTTCTTCGTg  >  1:901558/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCCGCCAATAAAGTTGTCATAGCGGGCTTTTAACTTGAGGGGGAAACCATACTCGCCGGGCTTAATCTGTGCTGAAGGGGGATTATTGGTCATGATCGTCTCCTTGCGGTGTGAGGTATAACAAGGGTAGACGTGACTGGCGAAATCTTCGCCAGTCGGTAACAGCTTTACGACAGCTATCACGAATTTACGGGCAAGGGTTACAGAGATAGTTGAAAAAGCGGCTAACAATTTGCCAGCCGTTGTGGAAATGATGATTACATCGCTGCGCGATAAATCGCCACAATTTCTTCGTG  >  NC_000913/3756418‑3756714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: