Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,051 C→G 6.8% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0510CG 6.8% 202.3 / 5.3 72intergenic (+34/‑164)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (27/37);  new base G (2/3);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCC  >  NC_000913/3780903‑3781196
                                                                                                                                                    |                                                                                                                                                 
gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCa                                                                                                                                                  >  2:960137/1‑150 (MQ=255)
gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                                                  <  1:461020/150‑1 (MQ=255)
  cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTACCTCAgg                                                                                                                                                >  2:262129/1‑150 (MQ=255)
   gCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAggg                                                                                                                                               <  1:1618935/150‑1 (MQ=255)
     tGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGgag                                                                                                                                             <  2:616940/150‑1 (MQ=255)
          ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggt                                                                                                                                        <  1:1270456/150‑2 (MQ=255)
          ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggt                                                                                                                                        <  1:595504/150‑2 (MQ=255)
          ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgggg                                                                                                                                        <  2:743735/150‑1 (MQ=255)
          ggCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCCCACCCACAGGGGGAgggg                                                                                                                                        >  2:1252436/1‑150 (MQ=255)
             gcgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttag                                                                                                                                     >  1:957131/1‑146 (MQ=255)
              cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCAAACCCACTCCCGCAGGGAGAGGGGTCCg                                                                                                                                    >  1:624719/1‑150 (MQ=255)
               gAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgggttaggg                                                                                                                                   <  2:1533988/150‑7 (MQ=255)
                aaaTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTCGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGtt                                                                                                                                  >  1:44794/1‑150 (MQ=255)
                aaaTCGATCAGCGAAATAACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCCCCCCCACCCCCAAACCCACACCCGCCAGGCGAGGGGGccccg                                                                                                                                  >  1:1378977/1‑147 (MQ=255)
                 aaTCGAGCAGCGAAATTACGCAAGATTCGCGGGTGCAGGGGCGGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCCTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGCGAGGGGACCGTTc                                                                                                                                 <  2:116746/150‑1 (MQ=255)
                     gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGgcg                                                                                                                             >  2:24946/1‑150 (MQ=255)
                          gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgta                                                                                                                        <  2:905026/150‑1 (MQ=255)
                            gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                                                      <  2:984185/150‑1 (MQ=255)
                             acaTTACGCAAGAGTCGCTGGGGCAGGGGCTGGGGAAAGAGGTGCCTGCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCTGGGAGAGGGGACCGTTCGGCGCtgtatgt                                                                                                                     <  1:310278/148‑1 (MQ=255)
                                ccgatctaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACt                                                                                                                  <  1:627594/143‑1 (MQ=255)
                                   cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCAAACCCTCTCCCGAACGGAGCGGGGACCGTTGGGCGCtgtttgtaaaccc                                                                                                               >  1:504401/1‑145 (MQ=255)
                                   cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                                               >  1:770916/1‑150 (MQ=255)
                                   cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAAACCACAACCACAGGGAGACGGGGCCCTTAGGCGCAGTATGGAATccc                                                                                                               >  2:1373297/1‑150 (MQ=255)
                                    gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                                              <  2:1081192/150‑1 (MQ=255)
                                    gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                                              <  2:1719291/150‑1 (MQ=255)
                                       aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTACCGCAGGGAGAGGGGACCGTTGGGCGCTGTATGTACtagatcgg                                                                                                           >  2:627594/1‑143 (MQ=255)
                                       aGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACTCCCAAAGGGAGAGGGGACCCTTAGGCGCTGTATGTACTACCacac                                                                                                           >  2:409622/1‑150 (MQ=255)
                                        gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCACTACCGGAGGGAGAGGGGGCCGTTCGGCGCGGGATGTACTCCCTCAcg                                                                                                          >  1:198697/1‑149 (MQ=255)
                                          ttCGCTGGTGCAGGGGGTGGGGATCGAGTTGCCTGCGGCACTGGCTCCCATGGCGATAGGGTATGCGGCATAGGCGTTTGCGTCTCCTGACCCTAACCCTCTCCCTCAGGGAGAGGTGAGCGTTCGGCGCTGTATGTACTCCctctctct                                                                                                        <  1:217446/150‑1 (MQ=255)
                                              cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGGTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                    <  1:74896/150‑1 (MQ=255)
                                                 gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTGGCGCCCCCGCACCAGAACCCTCTCCCGCAGGGAGAGGGGACCCTTAGGAGCCGTAGGTCCTACCCACGACGAAACGac                                                                                                >  1:907489/1‑150 (MQ=255)
                                                    cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGacac                                                                                              <  1:748921/150‑1 (MQ=255)
                                                        ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCGGTATGTACTCCCTCACTCTGAAACGACACCGcc                                                                                          >  1:290936/1‑149 (MQ=255)
                                                         gCTAGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGGTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCAc                                                                                         <  1:679427/150‑1 (MQ=255)
                                                           ggggTAAAGAGTGGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActc                                                                                       <  2:307869/149‑1 (MQ=255)
                                                                  agagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCTAAACCAATACCAAAGCGAAAGGGGACCCTTAGGCCCTGTATTTACTCCCACACTCAGAAACCAAACCCCACAAgtttttt                                                                                >  2:477899/1‑150 (MQ=255)
                                                                    agTTGCCTGCGGCACTGGCTCCCCTGGCGACAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCTAACCCTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCAGTATGTACTCCCTCACTCTGCAACGACACCGCCCTGGTTTTTtca                                                                              >  1:290498/1‑149 (MQ=255)
                                                                     gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                             <  1:761624/150‑1 (MQ=255)
                                                                      ttGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCCCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTcc                                                                            <  1:1606617/150‑1 (MQ=255)
                                                                       tGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCCCTCTGAAACGACACCGCACTCTTTTTTTCTccc                                                                           >  2:217219/1‑150 (MQ=255)
                                                                         ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCAATACCACTCCCTCAGGGAGAGGGGACCGTTAGGCGCTGTATGTACTCCCTCCCTCTTAAACGACACCGCACTCtttttttaacccac                                                                         >  1:729806/1‑143 (MQ=255)
                                                                          cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCCCCCTAACCCTCGCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACCCCGCCCTCTTTTTTTCTCCcgcg                                                                        >  1:1018809/1‑150 (MQ=255)
                                                                          cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                        >  2:979084/1‑150 (MQ=255)
                                                                               gctcTGGCTGCCTAGGCGAAAGCGAATGCGGCATAGTCCTTTGCGCCCCCTCTCCCTAACCCGCTCCCTCTGGGTGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACTCCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                   <  2:116322/147‑1 (MQ=255)
                                                                               gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                   <  2:275584/150‑1 (MQ=255)
                                                                                cACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTccccgg                                                                  <  2:992484/150‑3 (MQ=255)
                                                                                 aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCCCTCTTTTTTTCTCCCTCGCCCCGcc                                                                 >  1:927195/1‑150 (MQ=255)
                                                                                    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                              <  1:1530682/150‑1 (MQ=255)
                                                                                     gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                             <  1:1252436/150‑1 (MQ=255)
                                                                                      cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCGCAGGGAGAGGGGACCGATAGGCGCGGTATGTACTCCCGCACACAGAAACCCCACCCCACGCATTTTTTATCCCTCGCCCCGCAggggg                                                            >  2:513923/1‑149 (MQ=255)
                                                                                        cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                          <  1:719554/150‑1 (MQ=255)
                                                                                         ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                         >  1:1029958/1‑150 (MQ=255)
                                                                                            tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                      <  1:1450610/150‑2 (MQ=255)
                                                                                             ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgggag                                                     <  2:770916/150‑3 (MQ=255)
                                                                                               gaaaggggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                   >  1:900036/6‑150 (MQ=255)
                                                                                                gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                  >  2:37150/1‑150 (MQ=255)
                                                                                                  aaGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                <  1:1445761/150‑1 (MQ=255)
                                                                                                   aGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                               <  1:1497933/150‑1 (MQ=255)
                                                                                                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTATCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGTGAGGGCCGGGGTgg                                              >  1:962526/1‑149 (MQ=255)
                                                                                                     ggAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                             <  2:416727/150‑1 (MQ=255)
                                                                                                       aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTTTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                           <  2:514596/150‑1 (MQ=255)
                                                                                                            ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                      <  2:900036/150‑1 (MQ=255)
                                                                                                              cATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                    <  1:979084/150‑1 (MQ=255)
                                                                                                               aacggcGTTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGATAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGCCACCGCACGCATTTAAACTCCCTCCCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGc                                   <  2:100041/145‑1 (MQ=255)
                                                                                                                  gTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTGGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCGCGCCCCTCGGGGGAGAGGGCGGGGGTGAGGGTAAAAGGCCGc                                <  2:44794/150‑1 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc               >  1:644836/1‑150 (MQ=255)
                                                                                                                                    cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt              >  2:987607/1‑150 (MQ=255)
                                                                                                                                     catAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg             <  2:198697/148‑1 (MQ=255)
                                                                                                                                            ctctCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACTACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt      <  2:290498/150‑1 (MQ=255)
                                                                                                                                            ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt      >  2:976708/1‑150 (MQ=255)
                                                                                                                                            cgatcCCTCAGGGAGCGGGGACCGTGCGGCGCTGTATGCACTCCCTCCCGCTGCACCGCCCCCGCACTCTTTTTTTCTCCCTCGCCCCTCTGTGGAGTGGGCCGGGTTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt      <  1:883705/147‑1 (MQ=255)
                                                                                                                                                cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc  >  1:461171/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCC  >  NC_000913/3780903‑3781196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: