Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,131 T→G 13.7% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1310TG13.7% 143.4 / 8.1 67intergenic (+114/‑84)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (32/25);  new base G (6/4);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTT  >  NC_000913/3780982‑3781278
                                                                                                                                                      |                                                                                                                                                   
gctcTGGCTGCCTAGGCGAAAGCGAATGCGGCATAGTCCTTTGCGCCCCCTCTCCCTAACCCGCTCCCTCTGGGTGAGGGGACCGGTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACTCCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                                                                                                     <  2:116322/147‑1 (MQ=255)
gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCt                                                                                                                                                     <  2:275584/150‑1 (MQ=255)
  aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCCCTCTTTTTTTCTCCCTCGCCCCGcc                                                                                                                                                   >  1:927195/1‑150 (MQ=255)
     ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                <  1:1530682/150‑1 (MQ=255)
      gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                                                                                               <  1:1252436/150‑1 (MQ=255)
       cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCGCAGGGAGAGGGGACCGATAGGCGCGGTATGTACTCCCGCACACAGAAACCCCACCCCACGCATTTTTTATCCCTCGCCCCGCAggggg                                                                                                                                              >  2:513923/1‑149 (MQ=255)
         cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                            <  1:719554/150‑1 (MQ=255)
          ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                                                           >  1:1029958/1‑150 (MQ=255)
             tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                                                                                        <  1:1450610/150‑2 (MQ=255)
              ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgggag                                                                                                                                       <  2:770916/150‑3 (MQ=255)
                gaaaggggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                                                     >  1:900036/6‑150 (MQ=255)
                 gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                    >  2:37150/1‑150 (MQ=255)
                   aaGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                                                                  <  1:1445761/150‑1 (MQ=255)
                    aGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                                                                                                 <  1:1497933/150‑1 (MQ=255)
                     gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTATCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGTGAGGGCCGGGGTgg                                                                                                                                >  1:962526/1‑149 (MQ=255)
                      ggAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                               <  2:416727/150‑1 (MQ=255)
                        aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTTTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                             <  2:514596/150‑1 (MQ=255)
                             ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                                                        <  2:900036/150‑1 (MQ=255)
                               cATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                                                                                                      <  1:979084/150‑1 (MQ=255)
                                aacggcGTTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGATAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGCCACCGCACGCATTTAAACTCCCTCCCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGc                                                                                                                     <  2:100041/145‑1 (MQ=255)
                                   gTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTGGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCGCGCCCCTCGGGGGAGAGGGCGGGGGTGAGGGTAAAAGGCCGc                                                                                                                  <  2:44794/150‑1 (MQ=255)
                                                    aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                                                 >  1:644836/1‑150 (MQ=255)
                                                     cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                >  2:987607/1‑150 (MQ=255)
                                                      catAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                               <  2:198697/148‑1 (MQ=255)
                                                             ctctCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACTACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                        <  2:290498/150‑1 (MQ=255)
                                                             ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                        >  2:976708/1‑150 (MQ=255)
                                                             cgatcCCTCAGGGAGCGGGGACCGTGCGGCGCTGTATGCACTCCCTCCCGCTGCACCGCCCCCGCACTCTTTTTTTCTCCCTCGCCCCTCTGTGGAGTGGGCCGGGTTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                        <  1:883705/147‑1 (MQ=255)
                                                                 cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                                                    >  1:461171/1‑150 (MQ=255)
                                                                         gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                                            >  1:1559089/1‑150 (MQ=255)
                                                                          agagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                                                           >  1:1082237/1‑150 (MQ=255)
                                                                               ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                      >  1:1390281/1‑150 (MQ=255)
                                                                                 aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                                                    <  2:1029958/150‑1 (MQ=255)
                                                                                  ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTAGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAg                                                                   <  2:1624947/150‑1 (MQ=255)
                                                                                  ccGTTCGGCGCTGTATGAACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCGCCCGCCCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                                                   <  1:37150/150‑1 (MQ=255)
                                                                                   ggtgcGGCGCTGTTTTTACTCCCTCCCTCTGAACCGACCCGGCACGCTTTTTTGCTCCCCCGCCCCGCCGGGGTGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAgg                                                                  <  1:24946/146‑2 (MQ=255)
                                                                                   ctttCGGCGCGTTTGTAACTCCCTCACTCTGCAAGGACCCCGAACTCTTTTTTTCTCCCCCCCCCCTGCGGGGTGTGGGGCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                  <  2:504401/148‑1 (MQ=255)
                                                                                       cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                              >  1:1076613/1‑150 (MQ=255)
                                                                                       cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                              >  2:387083/1‑150 (MQ=255)
                                                                                        ggCGCTGTATGTCCTCCCTCACTCGTAAACGACACCTCACTCTTTTTTTCTCCCGCCCCCCTCCGGGGCGAGGGCCGGGGCGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                                             <  1:262129/150‑1 (MQ=255)
                                                                                         gcgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTTCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                                            >  2:629937/1‑150 (MQ=255)
                                                                                               tatgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                                                      >  2:61707/1‑150 (MQ=255)
                                                                                                 tgtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                                                    >  2:1601120/1‑150 (MQ=255)
                                                                                                     aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAACGTACt                                                 >  1:703431/1‑150 (MQ=255)
                                                                                                      cTCCCTCACTCTGAAACGACACCGCACTATTTTTTTCTCGCTCGCCCCGCCGGGGAGAGGGCCGGGGTTAGTGGTAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGTGGGCAGTATGCTTAACATCGTAGtt                                                >  2:296872/1‑150 (MQ=255)
                                                                                                        cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCACGCACTAAGGGGGCAGTATGCTAAACCTCGTCCTTTa                                              >  1:283723/1‑150 (MQ=255)
                                                                                                           tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                           >  2:1468846/1‑150 (MQ=255)
                                                                                                                ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                      >  2:349174/1‑150 (MQ=255)
                                                                                                                 tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa                                     >  2:933692/1‑150 (MQ=255)
                                                                                                                   aaaCGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCGGGGGTGTGGGCGGGGGTGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa                                   <  2:644836/150‑1 (MQ=255)
                                                                                                                    aaCGACCCCGCACTCTTTTTTTCTCCCACGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt                                  <  2:1080799/150‑1 (MQ=255)
                                                                                                                         cacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                             >  2:479096/1‑150 (MQ=255)
                                                                                                                           ccGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGACGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                           >  1:1070729/1‑150 (MQ=255)
                                                                                                                             gCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaaa                         >  2:1310043/1‑150 (MQ=255)
                                                                                                                              cACTTTTTTTTTCTCCCTCGCCCCGCGGGGGTGTGGGCGGGGGTGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAAt                        <  2:1390281/150‑1 (MQ=255)
                                                                                                                                ctctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATAc                      >  2:1564163/1‑150 (MQ=255)
                                                                                                                                 tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                     <  1:61707/150‑1 (MQ=255)
                                                                                                                                  ctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAGGAACCAGATATTCCGCCAAATAGTg                    >  2:247885/1‑150 (MQ=255)
                                                                                                                                   tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGAGAGGGGATAAGGCAGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTCCGGGGGCAGGATGCTACACATCGTACTGGCCGCCCCAGACTTGCCGCCCGATACTgg                   >  1:227952/1‑150 (MQ=255)
                                                                                                                                    ttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                  <  1:1601120/150‑1 (MQ=255)
                                                                                                                                          ctcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAAGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatc            >  2:347076/1‑150 (MQ=255)
                                                                                                                                           tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca           >  2:110981/1‑150 (MQ=255)
                                                                                                                                           gcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca           >  1:932027/2‑150 (MQ=255)
                                                                                                                                           gcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca           >  2:1370296/2‑150 (MQ=255)
                                                                                                                                            cccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAGGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat          >  1:876284/1‑150 (MQ=255)
                                                                                                                                            cccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat          >  2:263057/1‑150 (MQ=255)
                                                                                                                                                   cccTCGGGGGTGTGTGCCGGTGTGTGGTTATAAGGCCGCACTTCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt   <  1:477899/150‑1 (MQ=255)
                                                                                                                                                   cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt   <  1:987607/150‑1 (MQ=255)
                                                                                                                                                   cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt   >  1:1291839/1‑150 (MQ=255)
                                                                                                                                                    ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt  >  2:1142444/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
GCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTT  >  NC_000913/3780982‑3781278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: