Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,781,133	C→A	7.7%	intergenic (+116/‑82)	lldD → / → trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,133	0	C	A	7.7%	175.5 / 2.7	66	intergenic (+116/‑82)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (34/22);  new base A (3/2);  total (37/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTT  >  NC_000913/3780984‑3781278                                                                                                                                                       |                                                                                                                                                  aCTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCGCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGCCACCGCCCTCTTTTTTTCTCCCTCGCCCCGcc                                                                                                                                                   >  1:927195/1‑150 (MQ=255)    ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                <  1:1530682/150‑1 (MQ=255)     gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                                                                                               <  1:1252436/150‑1 (MQ=255)      cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCAAACCCACTCCCGCAGGGAGAGGGGACCGATAGGCGCGGTATGT‑ACTCCCGCACACAGAAACCCCACCCCACGCATTTTTTATCCCTCGCCCCGCAggggg                                                                                                                                              >  2:513923/1‑149 (MQ=255)        cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                            <  1:719554/150‑1 (MQ=255)         ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                                                           >  1:1029958/1‑150 (MQ=255)            tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                                                                                                                                        <  1:1450610/150‑2 (MQ=255)             ggCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAgggag                                                                                                                                       <  2:770916/150‑3 (MQ=255)               gaaaggggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgg                                                                                                                                     >  1:900036/6‑150 (MQ=255)                gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                    >  2:37150/1‑150 (MQ=255)                  aaGGGAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                                                                  <  1:1445761/150‑1 (MQ=255)                   aGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                                                                                                 <  1:1497933/150‑1 (MQ=255)                    gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTATCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCGCCGGGGTGAGGGCCGGGGTgg                                                                                                                                >  1:962526/1‑149 (MQ=255)                     ggAATGCGGCATAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                               <  2:416727/150‑1 (MQ=255)                       aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTTTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                             <  2:514596/150‑1 (MQ=255)                            ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                                                                        <  2:900036/150‑1 (MQ=255)                              cATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                                                                                                      <  1:979084/150‑1 (MQ=255)                               aacggcGTTTGCGCCCCCTCACCCAAACCCTCTCCCTCAGGGATAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAACCGCCACCGCACGCATTTAAACTCCCTCCCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGc                                                                                                                     <  2:100041/145‑1 (MQ=255)                                  gTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTGGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCGCGCCCCTCGGGGGAGAGGGCGGGGGTGAGGGTAAAAGGCCGc                                                                                                                  <  2:44794/150‑1 (MQ=255)                                                   aCCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                                                 >  1:644836/1‑150 (MQ=255)                                                    cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                >  2:987607/1‑150 (MQ=255)                                                     catAACCCTCTCCCTCAGGGTGTGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                                                               <  2:198697/148‑1 (MQ=255)                                                            ctctCCCTCAGGGCGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACTACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                        <  2:290498/150‑1 (MQ=255)                                                            ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                        >  2:976708/1‑150 (MQ=255)                                                            cgatcCCTCAGGGAGCGGGGACCGTGCGGCGCTGTATGC‑ACTCCCTCCCGCTGCACCGCCCCCGCACTCTTTTTTTCTCCCTCGCCCCTCTGTGGAGTGGGCCGGGTTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                        <  1:883705/147‑1 (MQ=255)                                                                cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                                                    >  1:461171/1‑150 (MQ=255)                                                                        gagaGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                                                            >  1:1559089/1‑150 (MQ=255)                                                                         agagGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTaa                                                                           >  1:1082237/1‑150 (MQ=255)                                                                              ggACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                                                      >  1:1390281/1‑150 (MQ=255)                                                                                aCCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                                                                    <  2:1029958/150‑1 (MQ=255)                                                                                 ccGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTAGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAg                                                                   <  2:1624947/150‑1 (MQ=255)                                                                                 ccGTTCGGCGCTGTATGA‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCGCCCGCCCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                                                   <  1:37150/150‑1 (MQ=255)                                                                                  ggtgcGGCGCTGTTTTT‑ACTCCCTCCCTCTGAACCGACCCGGCACGCTTTTTTGCTCCCCCGCCCCGCCGGGGTGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCTTAGTGCGGGGGCAgg                                                                  <  1:24946/146‑2 (MQ=255)                                                                                  ctttCGGCGC‑GTTTGTAACTCCCTCACTCTGCAAGGACCCCGAACTCTTTTTTTCTCCCCCCCCCCTGCGGGGTGTGGGGCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                                                                  <  2:504401/148‑1 (MQ=255)                                                                                      cGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                              >  1:1076613/1‑150 (MQ=255)                                                                                      cGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGc                                                              >  2:387083/1‑150 (MQ=255)                                                                                       ggCGCTGTATGT‑CCTCCCTCACTCGTAAACGACACCTCACTCTTTTTTTCTCCCGCCCCCCTCCGGGGCGAGGGCCGGGGCGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                                                             <  1:262129/150‑1 (MQ=255)                                                                                        gcgcTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTTCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                                                            >  2:629937/1‑150 (MQ=255)                                                                                              tatgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                                                      >  2:61707/1‑150 (MQ=255)                                                                                                tgt‑aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                                                    >  2:1601120/1‑150 (MQ=255)                                                                                                    aCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACAACGTACt                                                 >  1:703431/1‑150 (MQ=255)                                                                                                     cTCCCTCACTCTGAAACGACACCGCACTATTTTTTTCTCGCTCGCCCCGCCGGGGAGAGGGCCGGGGTTAGTGGTAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGTGGGCAGTATGCTTAACATCGTAGtt                                                >  2:296872/1‑150 (MQ=255)                                                                                                       cccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCACGCACTAAGGGGGCAGTATGCTAAACCTCGTCCTTTa                                              >  1:283723/1‑150 (MQ=255)                                                                                                          tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                           >  2:1468846/1‑150 (MQ=255)                                                                                                               ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                      >  2:349174/1‑150 (MQ=255)                                                                                                                tGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa                                     >  2:933692/1‑150 (MQ=255)                                                                                                                  aaaCGACCCCGCACTCTTTTTTTCTCCCTCGCCCCTCGGGGGTGTGGGCGGGGGTGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGaaa                                   <  2:644836/150‑1 (MQ=255)                                                                                                                   aaCGACCCCGCACTCTTTTTTTCTCCCACGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAAt                                  <  2:1080799/150‑1 (MQ=255)                                                                                                                        cacCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGc                             >  2:479096/1‑150 (MQ=255)                                                                                                                          ccGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGACGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                           >  1:1070729/1‑150 (MQ=255)                                                                                                                            gCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCaaa                         >  2:1310043/1‑150 (MQ=255)                                                                                                                             cACTTTTTTTTTCTCCCTCGCCCCGCGGGGGTGTGGGCGGGGGTGCGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAAt                        <  2:1390281/150‑1 (MQ=255)                                                                                                                               ctctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATAc                      >  2:1564163/1‑150 (MQ=255)                                                                                                                                tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                     <  1:61707/150‑1 (MQ=255)                                                                                                                                 ctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAGGAACCAGATATTCCGCCAAATAGTg                    >  2:247885/1‑150 (MQ=255)                                                                                                                                  tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGAGAGGGGATAAGGCAGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTCCGGGGGCAGGATGCTACACATCGTACTGGCCGCCCCAGACTTGCCGCCCGATACTgg                   >  1:227952/1‑150 (MQ=255)                                                                                                                                   ttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGc                  <  1:1601120/150‑1 (MQ=255)                                                                                                                                         ctcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAAGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatc            >  2:347076/1‑150 (MQ=255)                                                                                                                                          tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca           >  2:110981/1‑150 (MQ=255)                                                                                                                                          gcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca           >  1:932027/2‑150 (MQ=255)                                                                                                                                          gcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca           >  2:1370296/2‑150 (MQ=255)                                                                                                                                           cccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAGGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat          >  1:876284/1‑150 (MQ=255)                                                                                                                                           cccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatcat          >  2:263057/1‑150 (MQ=255)                                                                                                                                                  cccTCGGGGGTGTGTGCCGGTGTGTGGTTATAAGGCCGCACTTCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt   <  1:477899/150‑1 (MQ=255)                                                                                                                                                  cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt   >  1:1291839/1‑150 (MQ=255)                                                                                                                                                  cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt   <  1:987607/150‑1 (MQ=255)                                                                                                                                                   ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt  >  2:1142444/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                  ACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGT‑ACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTT  >  NC_000913/3780984‑3781278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑