Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,895,235 C→G 5.2% intergenic (+17/+37) chrR → / ← adeP chromate reductase, Class I, flavoprotein/adenine permease, high affinity; adenine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,895,2350CG 5.2% 504.2 / 12.0 150intergenic (+17/+37)chrR/adePchromate reductase, Class I, flavoprotein/adenine permease, high affinity; adenine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (77/65);  new base G (3/5);  total (80/70)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 152 total aligned reads displayed.

GAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGA  >  NC_000913/3895087‑3895384
                                                                                                                                                    |                                                                                                                                                     
gAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCa                                                                                                                                                      >  1:981651/1‑150 (MQ=255)
   tttATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATc                                                                                                                                                   >  2:1475616/1‑150 (MQ=255)
     tATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATcat                                                                                                                                                 >  1:971245/1‑150 (MQ=255)
      aTGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATcatg                                                                                                                                                <  1:1560598/150‑1 (MQ=255)
       tGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATcatgc                                                                                                                                               >  2:829609/1‑150 (MQ=255)
              cgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCgg                                                                                                                                        >  1:729794/1‑150 (MQ=255)
              cgTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCgg                                                                                                                                        <  1:1249022/150‑1 (MQ=255)
                  aTTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCATGCATGATTGCTGGCGGGttt                                                                                                                                    <  2:466488/150‑1 (MQ=255)
                  aTTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGttt                                                                                                                                    >  1:1022844/1‑150 (MQ=255)
                  aTTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGttt                                                                                                                                    >  1:1390873/1‑150 (MQ=255)
                  aTTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAAGCATGCATGGCGGGttt                                                                                                                                    >  1:852523/1‑150 (MQ=255)
                   ttCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAATACCCGCCAGCAATCATGCATGGCGGGtttt                                                                                                                                   <  1:927088/150‑1 (MQ=255)
                   ttCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCATGCATGATTGCTGGCGGGtttt                                                                                                                                   <  2:1104927/150‑1 (MQ=255)
                   ttCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGtttt                                                                                                                                   >  2:1623629/1‑150 (MQ=255)
                   ttCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACAGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGtttt                                                                                                                                   >  2:859899/1‑150 (MQ=255)
                    tCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                                  >  2:1100961/1‑150 (MQ=255)
                    tCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGttttt                                                                                                                                  <  1:1621050/150‑1 (MQ=255)
                     cAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTa                                                                                                                                 >  2:764865/1‑150 (MQ=255)
                      aGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTaa                                                                                                                                >  2:1200371/1‑150 (MQ=255)
                       gAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAAc                                                                                                                               >  1:518125/1‑150 (MQ=255)
                       gAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCAAGGCGGGTTTTTAAc                                                                                                                               >  2:702755/1‑150 (MQ=255)
                       gAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGATACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAAc                                                                                                                               >  1:193658/1‑150 (MQ=255)
                         aCAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAAcgc                                                                                                                             <  1:175169/150‑1 (MQ=255)
                           aaaGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACgcgc                                                                                                                           >  2:1376283/1‑150 (MQ=255)
                              gTTGATCCGCAAACCGGAGAAGTTATTGATCATGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGGTTATTCAGCGAGTTAACATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTAt                                                                                                                        <  2:291665/150‑1 (MQ=255)
                                tGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGAATGGCGGGTTTTTAACGCTCTATCg                                                                                                                      >  1:1103369/1‑150 (MQ=255)
                                   tCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAtt                                                                                                                   <  2:415074/150‑1 (MQ=255)
                                   tCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAtt                                                                                                                   >  2:1487815/1‑150 (MQ=255)
                                     cGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAtttt                                                                                                                 >  1:1706428/1‑150 (MQ=255)
                                      gCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTa                                                                                                                >  1:379229/1‑150 (MQ=255)
                                       cAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAg                                                                                                               <  2:971245/150‑1 (MQ=255)
                                         aaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTg                                                                                                             >  2:529416/1‑150 (MQ=255)
                                         aaCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTg                                                                                                             <  1:1200371/150‑1 (MQ=255)
                                          aCCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGa                                                                                                            >  1:239970/1‑150 (MQ=255)
                                           ccGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAg                                                                                                           >  2:1114475/1‑150 (MQ=255)
                                               agaAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTc                                                                                                       <  1:802929/150‑1 (MQ=255)
                                               agaAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTc                                                                                                       >  1:1572211/1‑150 (MQ=255)
                                                gaAGTGATTGATCAGGGTACGCTGGCCCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCg                                                                                                      >  2:347544/1‑150 (MQ=255)
                                                 aaGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGa                                                                                                     <  2:702391/150‑1 (MQ=255)
                                                  aGTGATTGATCAGGGTACGCTGACCACCTGCCCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATa                                                                                                   >  2:30435/1‑150 (MQ=255)
                                                     gattgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATaaa                                                                                                 <  1:1270671/150‑1 (MQ=255)
                                                        tgatCAGGTTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGAc                                                                                              <  1:1171029/150‑1 (MQ=255)
                                                        tgatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGAc                                                                                              <  2:891025/150‑1 (MQ=255)
                                                         gatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACa                                                                                             >  1:576070/1‑150 (MQ=255)
                                                         gatCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACa                                                                                             >  1:382631/1‑150 (MQ=255)
                                                            cAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATc                                                                                          <  2:664356/150‑1 (MQ=255)
                                                              gggTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCtt                                                                                        >  2:1277144/1‑150 (MQ=255)
                                                                 tACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAg                                                                                     <  2:1667666/150‑1 (MQ=255)
                                                                  aCGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTTAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAgg                                                                                    >  1:136081/1‑150 (MQ=255)
                                                                   cGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGa                                                                                   <  2:1287590/150‑1 (MQ=255)
                                                                    gCTGGACCACCTGACCTGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGAt                                                                                  <  2:241351/150‑1 (MQ=255)
                                                                    gCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAATATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGAt                                                                                  <  1:1063049/150‑1 (MQ=255)
                                                                             ccTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAAcagcag                                                                         <  2:651529/150‑1 (MQ=255)
                                                                               tGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGATTTAAGATCTAAATAAAAAACCCGCCTGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAgcg                                                                       <  2:724653/150‑1 (MQ=255)
                                                                               tGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACATCAgcg                                                                       >  1:169531/1‑150 (MQ=255)
                                                                                       cAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACgatg                                                               >  2:949911/1‑150 (MQ=255)
                                                                                           tGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTa                                                           <  1:1507925/150‑1 (MQ=255)
                                                                                           tGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTa                                                           >  1:618417/1‑150 (MQ=255)
                                                                                           tGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTa                                                           <  1:1376823/150‑1 (MQ=255)
                                                                                                gCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCAc                                                      >  2:145447/1‑150 (MQ=255)
                                                                                                 cATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACg                                                     <  1:678711/150‑1 (MQ=255)
                                                                                                  aTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACgg                                                    <  2:578584/150‑1 (MQ=255)
                                                                                                  aTTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACggg                                                   >  2:75410/1‑150 (MQ=255)
                                                                                                   tttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACggg                                                   >  1:1469749/1‑150 (MQ=255)
                                                                                                   tttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACggg                                                   <  1:814975/150‑1 (MQ=255)
                                                                                                    ttGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGc                                                  <  2:239970/150‑1 (MQ=255)
                                                                                                     tGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCt                                                 <  1:1277144/150‑1 (MQ=255)
                                                                                                       gTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                               >  1:518368/1‑150 (MQ=255)
                                                                                                       gTGAGTTTATTCAGAGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTa                                               <  1:1035688/150‑1 (MQ=255)
                                                                                                        tGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAg                                              <  1:1623629/150‑1 (MQ=255)
                                                                                                          tgTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGAt                                            <  1:1242214/149‑1 (MQ=255)
                                                                                                            tttATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCa                                          <  1:619975/150‑1 (MQ=255)
                                                                                                              tATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACg                                        <  1:781013/150‑1 (MQ=255)
                                                                                                              tATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACg                                        >  2:510693/1‑150 (MQ=255)
                                                                                                                ttCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCa                                      >  2:787263/1‑150 (MQ=255)
                                                                                                                ttCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCa                                      <  2:717943/150‑1 (MQ=255)
                                                                                                                  cAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGa                                    >  2:105300/1‑150 (MQ=255)
                                                                                                                  cAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGa                                    <  1:1457107/150‑1 (MQ=255)
                                                                                                                     cGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGt                                 >  1:1355684/1‑150 (MQ=255)
                                                                                                                        gTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGAGTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCg                              <  1:870191/150‑1 (MQ=255)
                                                                                                                          tAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCCCGGGCTTAGATCACGCAGACGTCCGGt                            >  1:545027/1‑150 (MQ=255)
                                                                                                                          tAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGt                            >  1:757064/1‑150 (MQ=255)
                                                                                                                          tAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCAGGt                            >  2:1506324/1‑150 (MQ=255)
                                                                                                                           aaGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTa                           <  1:458647/150‑1 (MQ=255)
                                                                                                                             gATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTAcc                         >  1:433498/1‑150 (MQ=255)
                                                                                                                               tCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCCGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGa                       >  2:1411954/1‑150 (MQ=255)
                                                                                                                                   aataaaAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGAtttt                   >  2:1372247/1‑150 (MQ=255)
                                                                                                                                     taaaAACCCCGGCAGCAATCATTCATGTCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtca                 <  1:106761/150‑1 (MQ=255)
                                                                                                                                       aaaaaCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtcctc               >  2:1564705/1‑150 (MQ=255)
                                                                                                                                       aaaaaCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtcatc               >  2:1629626/1‑150 (MQ=255)
                                                                                                                                        aaaaCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTtcatct              <  1:30435/150‑2 (MQ=255)
                                                                                                                                        aaaaCCCGCCAGCAATCAAGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCATGCAGACGTCCGGTACCGATTTtcatca              >  2:1192317/1‑150 (MQ=255)
                                                                                                                                          aaCCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCATCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATTATTACGCACGGTCTTAGATCACTCATACTGCCTGTACCAATTTTCATCACg            >  1:251904/1‑150 (MQ=255)
                                                                                                                                            cccGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCa          >  1:1659973/1‑150 (MQ=255)
                                                                                                                                             ccGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAg         <  2:868287/150‑1 (MQ=255)
                                                                                                                                              cGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGt        >  1:220725/1‑150 (MQ=255)
                                                                                                                                              cGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGt        >  1:1587622/1‑150 (MQ=255)
                                                                                                                                                 cAGCAATCAAGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAATGATGATTACGCACTGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAgg     <  2:1129253/150‑1 (MQ=255)
                                                                                                                                                    cAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGgaga  <  1:1106397/150‑1 (MQ=255)
                                                                                                                                                    cAATCATGCAAGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGgaga  <  1:702755/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAATTTATGGGCGGCGTGATTCAGAACAAAGTTGATCCGCAAACCGGAGAAGTGATTGATCAGGGTACGCTGGACCACCTGACCGGGCAATTGACCGCATTTGGTGAGTTTATTCAGCGAGTTAAGATCTAAATAAAAAACCCGCCAGCAATCATGCATGGCGGGTTTTTAACGCGCTATCGATTTTAGTGAGCGTCGATAAAGACAATCTTCAGGATAAACAGCAGCGCAACGATGATTACGCACGGGCTTAGATCACGCAGACGTCCGGTACCGATTTTCATCACGCAGTAGGAGA  >  NC_000913/3895087‑3895384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: