Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,897,613 G→A 8.4% P36P (CCG→CCA cbrB → PRK09823 family inner membrane protein, creBC regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,897,6130GA 8.4% 469.6 / 20.3 156P36P (CCG→CCAcbrBPRK09823 family inner membrane protein, creBC regulon
Reads supporting (aligned to +/- strand):  ref base G (58/83);  new base A (8/5);  total (67/89)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-02

Only 100 of 156 total aligned reads displayed.

GCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGC  >  NC_000913/3897466‑3897760
                                                                                                                                                   |                                                                                                                                                   
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                   <  2:1434845/150‑1 (MQ=255)
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                   >  1:1425412/1‑150 (MQ=255)
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                   <  1:1190655/150‑1 (MQ=255)
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                   >  2:827873/1‑150 (MQ=255)
gCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                   <  1:471365/150‑1 (MQ=255)
gCTTAAATTGGCGCTCAAAGGGAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGCGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCt                                                                                                                                                   <  2:1627550/150‑1 (MQ=255)
 cTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTg                                                                                                                                                  >  2:32169/1‑150 (MQ=255)
  ttAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTgg                                                                                                                                                 <  2:1495093/150‑1 (MQ=255)
    aaaTTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGtt                                                                                                                                               >  2:488714/1‑150 (MQ=255)
         ggCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTg                                                                                                                                          <  2:1034930/150‑1 (MQ=255)
          gcgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCttttg                                                                                                                                         <  2:489900/150‑3 (MQ=255)
           cgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCttttgc                                                                                                                                        <  2:1633279/150‑4 (MQ=255)
           cgcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTGCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCAGCGGTTCttttgc                                                                                                                                        <  1:1363940/150‑4 (MQ=255)
            tggcAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                       <  1:1270914/147‑1 (MQ=255)
            gcTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGt                                                                                                                                       <  2:1574512/150‑1 (MQ=255)
             cTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTg                                                                                                                                      <  1:993431/150‑1 (MQ=255)
              tCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGTACCGTTAATAGGTGTTCTGTTTCTTGTCCTCTACATATTATTCGCAAAAGAACCGCTGGTAATATGGGTGa                                                                                                                                     >  2:389092/1‑150 (MQ=255)
                aaaGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTTATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGata                                                                                                                                   <  1:1378535/150‑1 (MQ=255)
                   ggTAAGTAAAGGGAGTTTGATATGTCTGTTTCAGGTCGGGTAATACATCACGGACTTTATTTTGCAGTTGTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGataata                                                                                                                                <  1:828171/150‑1 (MQ=255)
                   ggTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGTATCGTTAATTGGTGTTCTGTTTCTTGTCCTCTAAATATTCTTCGCAAAAGAAACGATGGTTCTTTGGGTGataata                                                                                                                                >  1:202277/1‑150 (MQ=255)
                   ggTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGataata                                                                                                                                <  1:1157604/150‑1 (MQ=255)
                      aagtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACAt                                                                                                                             >  2:596317/1‑150 (MQ=255)
                       tgtatAGGGAGTTTGATATGTCTGTTTGACTTCTGGTAATACATCAATTACTTTATTTTGCAGTTTTATGACCGTTATTTGTTGTTATGTTTCTTGTCCTATACATATTCTTCGCATAAGATGCGCTGGTTATTTGGGTGATAATACATc                                                                                                                            <  2:1285622/149‑1 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATc                                                                                                                            >  1:745129/1‑150 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATc                                                                                                                            <  1:1593014/150‑1 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATc                                                                                                                            <  1:1415056/150‑1 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATc                                                                                                                            >  2:629794/1‑150 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATc                                                                                                                            <  2:711575/150‑1 (MQ=255)
                       agtaaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATc                                                                                                                            <  1:1249232/150‑1 (MQ=255)
                        gtaaAGGGAGTTTGATATGTCTTTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTATAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATcc                                                                                                                           <  1:324907/150‑1 (MQ=255)
                         taaAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCa                                                                                                                          <  2:11192/150‑1 (MQ=255)
                               gAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATAAATCAAAttttt                                                                                                                    >  2:1604272/1‑150 (MQ=255)
                                     gATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTa                                                                                                              >  2:1021855/1‑150 (MQ=255)
                                      atatGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTACTCTACATATTCTTCGCAAAAGAACCGCTGGATCTTTGGGTGATAATACATCCAATTTTTCTCTTAt                                                                                                             >  1:1498436/1‑150 (MQ=255)
                                      atatCTCTGTTTCACGTCGGGTAATACATAACCGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTACTCTACATATTCTTCGCAAAAGAACCGCTTGTTCTTTGGTTTATAATACATCCAATTTTTCACTTAt                                                                                                             >  2:819309/1‑150 (MQ=255)
                                          gTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTc                                                                                                         <  1:1017987/150‑1 (MQ=255)
                                           tCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCg                                                                                                        >  2:1593960/1‑150 (MQ=255)
                                              gTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATa                                                                                                     <  2:1418086/150‑1 (MQ=255)
                                              gTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACAGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATa                                                                                                     >  1:171177/1‑150 (MQ=255)
                                               tttCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATaa                                                                                                    <  2:224319/150‑1 (MQ=255)
                                                 tCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACa                                                                                                                              <  1:1705550/122‑1 (MQ=255)
                                                 tCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACt                                                                                                  >  2:1331854/1‑150 (MQ=255)
                                                   aCGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTAc                                                                                                <  2:416630/150‑1 (MQ=255)
                                                   aCGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTAc                                                                                                <  2:372726/150‑1 (MQ=255)
                                                    cGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACg                                                                                               <  2:1425412/150‑1 (MQ=255)
                                                     gTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACgg                                                                                              >  1:1293664/1‑150 (MQ=255)
                                                        gggTAATACCTCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAg                                                                                           <  2:1374223/150‑1 (MQ=255)
                                                        gggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCTCAAAAGAACCGCTGGTTCTTTGTGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAg                                                                                           <  2:30379/150‑1 (MQ=255)
                                                         ggTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGc                                                                                          <  2:1310562/150‑1 (MQ=255)
                                                          gTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCt                                                                                         >  1:668981/1‑150 (MQ=255)
                                                               aCATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTcc                                                                                    >  2:862050/1‑150 (MQ=255)
                                                                cATCACTGACTTTATTTTGCATTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTATACATATTCTTCGCAAAAGAACCGCTGGTTCTTTTGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCt                                                                                   <  1:426728/150‑1 (MQ=255)
                                                                    aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTGCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGTTGAGAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgt                                                                               >  2:303389/1‑150 (MQ=255)
                                                                    aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgt                                                                               <  1:303981/150‑1 (MQ=255)
                                                                    aCGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCATCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgt                                                                               >  1:699366/1‑150 (MQ=255)
                                                                      ggACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCgttg                                                                             <  1:1021855/150‑1 (MQ=255)
                                                                         cTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTa                                                                          >  2:1089326/1‑150 (MQ=255)
                                                                         cTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTATTATTGTCGATAACTAAGGGAGCTATTCCTGCGTTGTTa                                                                          >  1:751432/1‑150 (MQ=255)
                                                                           ttATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAAc                                                                        >  2:551899/1‑150 (MQ=255)
                                                                            tATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAAcc                                                                       <  1:629794/150‑1 (MQ=255)
                                                                            tATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCg                                                                      >  2:753453/1‑150 (MQ=255)
                                                                             aTTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCgg                                                                     <  2:404227/150‑1 (MQ=255)
                                                                                   cAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTaa                                                                >  2:1709546/1‑150 (MQ=255)
                                                                                    aGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGAGAATACATGCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAAt                                                               >  2:1075257/1‑150 (MQ=255)
                                                                                     gTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTATCTTATTGTCGATAACTACGGGAGCTATTACTGAGTTTTTAACCGGTGTAATg                                                              >  2:1385291/1‑150 (MQ=255)
                                                                                      ttttAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATgg                                                             <  2:315903/150‑1 (MQ=255)
                                                                                         tAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAAACGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTg                                                          <  2:883718/150‑1 (MQ=255)
                                                                                           ggACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGgtg                                                                   >  1:1378931/1‑139 (MQ=255)
                                                                                            gACCGTTATTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCt                                                       <  1:827873/150‑1 (MQ=255)
                                                                                                   aaTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGcc                                                <  1:32169/150‑1 (MQ=255)
                                                                                                   aaTTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGGTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGcc                                                <  1:382906/150‑1 (MQ=255)
                                                                                                          gtTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAg                                         <  1:862050/150‑1 (MQ=255)
                                                                                                                  tCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTc                                 <  1:409064/150‑1 (MQ=255)
                                                                                                                  tCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTc                                 <  2:635470/150‑1 (MQ=255)
                                                                                                                    ttGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTcac                               <  1:1192500/150‑1 (MQ=255)
                                                                                                                      gTCCTCTACATATTCTTCGCAAAAGAACCTCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAg                             >  1:510788/1‑150 (MQ=255)
                                                                                                                        ccTCTACATATTCTTCGCAAAAGAACCACTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGaa                           >  1:246877/1‑150 (MQ=255)
                                                                                                                         ctctACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGaaa                          <  2:668981/150‑1 (MQ=255)
                                                                                                                            tACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGt                       >  2:974884/1‑150 (MQ=255)
                                                                                                                                tatTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGaaaa                                          <  2:807771/127‑1 (MQ=255)
                                                                                                                                tatTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGaaaa                                          >  1:807771/1‑127 (MQ=255)
                                                                                                                                    cttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTg               <  1:1240389/150‑1 (MQ=255)
                                                                                                                                    attcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTg               <  1:162035/149‑1 (MQ=255)
                                                                                                                                     ttcGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGt              <  2:1263687/150‑1 (MQ=255)
                                                                                                                                      cacccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTc             >  1:469177/6‑150 (MQ=255)
                                                                                                                                      cacccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTc             >  1:1582811/6‑150 (MQ=255)
                                                                                                                                        gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTc             >  1:355050/1‑148 (MQ=255)
                                                                                                                                        gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTc             <  2:355050/148‑1 (MQ=255)
                                                                                                                                        gCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGGTGCCTGGCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGGTGTctg           <  1:1359543/150‑1 (MQ=255)
                                                                                                                                        cccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCTATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTg               <  1:919218/143‑1 (MQ=255)
                                                                                                                                        cccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCTATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTg               >  2:919218/4‑146 (MQ=255)
                                                                                                                                        cccaaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctg           >  1:1683161/4‑150 (MQ=255)
                                                                                                                                         cAAAAGAAGCGCTGTTTATTTGGGTTATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTTCGTTGTTAACCGGTTTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctgg          <  2:175420/150‑1 (MQ=255)
                                                                                                                                         cAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctgg          <  2:1520416/150‑1 (MQ=255)
                                                                                                                                          caaaGAACCAGCGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc         >  1:696967/2‑150 (MQ=255)
                                                                                                                                          caaaGAACCAGCGGTTCTTTGGGTGATAATACATACAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTctggc         >  1:703180/2‑150 (MQ=255)
                                                                                                                                             aGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCGTAGTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTTACCGGTGTAATGGGTGCCTGTCTGCCGGAAAAGCTCGGGGCCCAGAATCGTTATCGGGGGctggctgg      >  2:1148385/1‑150 (MQ=255)
                                                                                                                                              taaCCTCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATATCTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCtggt     <  1:596317/149‑1 (MQ=255)
                                                                                                                                               aaCCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCTGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCtggtg    <  1:1315208/150‑1 (MQ=255)
                                                                                                                                                 ccGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGc  >  1:1086891/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTTAAATTGGCGCTCAAAGGTAAGTAAAGGGAGTTTGATATGTCTGTTTCACGTCGGGTAATACATCACGGACTTTATTTTGCAGTTTTAGGACCGTTAATTGGTGTTCTGTTTCTTGTCCTCTACATATTCTTCGCAAAAGAACCGCTGGTTCTTTGGGTGATAATACATCCAATTTTTCTCTTATTGTCGATAACTACGGGAGCTATTCCTGCGTTGTTAACCGGTGTAATGGTTGCCTGTCTGCCGGAAAAGATCGGGTCACAGAAACGTTATCGTTGTCTGGCTGGTGGC  >  NC_000913/3897466‑3897760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: