Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,908,435 T→G 5.5% intergenic (‑69/+114) pstB ← / ← pstA phosphate ABC transporter ATPase/phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,908,4350TG 5.5% 350.0 / 8.5 126intergenic (‑69/+114)pstB/pstAphosphate ABC transporter ATPase/phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (45/73);  new base G (3/4);  total (49/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 126 total aligned reads displayed.

TTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCG  >  NC_000913/3908287‑3908581
                                                                                                                                                    |                                                                                                                                                  
ttCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTc                                                                                                                                                   >  2:1281548/1‑150 (MQ=255)
 tCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCa                                                                                                                                                  <  1:175018/150‑1 (MQ=255)
  cAGGGCCTGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAc                                                                                                                                                 <  1:1154020/150‑1 (MQ=255)
  cAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAc                                                                                                                                                 <  1:464299/150‑1 (MQ=255)
   cgggCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCCTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGTCCGGATCAGGCGTTCAcg                                                                                                                                                <  2:282526/149‑1 (MQ=255)
   aGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcg                                                                                                                                                <  1:571844/150‑1 (MQ=255)
   aGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcg                                                                                                                                                <  2:116967/150‑1 (MQ=255)
    gggCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgc                                                                                                                                               >  1:552224/1‑150 (MQ=255)
    gggCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgc                                                                                                                                               >  2:1223944/1‑150 (MQ=255)
      gCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCTATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                             <  1:191182/150‑1 (MQ=255)
      gCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                             <  2:337037/150‑1 (MQ=255)
      gCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                             <  2:965959/150‑1 (MQ=255)
      gCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                             <  1:167013/150‑1 (MQ=255)
      gCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                             >  1:1251460/1‑150 (MQ=255)
      gCAGGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTCCTCGGGTCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCAcgccg                                                                                                                                             <  1:271023/150‑1 (MQ=255)
         tGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCAt                                                                                                                                          >  2:1219623/1‑150 (MQ=255)
         tGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCAt                                                                                                                                          <  1:1214565/150‑1 (MQ=255)
         tGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCAt                                                                                                                                          >  1:404352/1‑150 (MQ=255)
             aTTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATGCGGCGTGAACGCCTGATCCgg                                                                                                                                      <  2:1552112/150‑1 (MQ=255)
                tGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCaa                                                                                                                                   >  1:1555901/1‑150 (MQ=255)
                  ccGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAg                                                                                                                                 >  1:973410/1‑150 (MQ=255)
                    gtagtaGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                               <  1:1562464/150‑1 (MQ=255)
                    gtagtaGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtg                                                                                                                               <  1:405148/150‑1 (MQ=255)
                     tagtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAgtgt                                                                                                                              <  2:886495/150‑1 (MQ=255)
                      agtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGtt                                                                                                                             <  2:630963/150‑1 (MQ=255)
                      agtagAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGtt                                                                                                                             <  1:1018786/150‑1 (MQ=255)
                         agAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAAc                                                                                                                          <  2:5512/150‑1 (MQ=255)
                         agAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGATTCAACCATACTCATAGCAATCTTATCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAAc                                                                                                                          <  1:289229/150‑1 (MQ=255)
                           aaGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCg                                                                                                                        <  2:815211/150‑1 (MQ=255)
                           aaGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCg                                                                                                                        <  2:1585704/150‑1 (MQ=255)
                           aaGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTGCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCg                                                                                                                        <  1:1373453/150‑1 (MQ=255)
                             gTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgtg                                                                                                                      <  2:1256938/150‑1 (MQ=255)
                              ttCTAATTACGCACCTGTATTTTACTCTGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCATAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCgtgt                                                                                                                     <  2:1039032/150‑1 (MQ=255)
                               tCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTa                                                                                                                    >  1:1622418/1‑150 (MQ=255)
                                cAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTAc                                                                                                                   <  2:1197662/150‑1 (MQ=255)
                                cAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTAc                                                                                                                   >  2:113794/1‑150 (MQ=255)
                                cAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTAc                                                                                                                   <  1:1281548/150‑1 (MQ=255)
                                     tACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGt                                                                                                              >  2:1013481/1‑150 (MQ=255)
                                             tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAgg                                                                                                      >  2:632373/1‑150 (MQ=255)
                                             tGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAgg                                                                                                      <  2:1509869/150‑1 (MQ=255)
                                             tGAATTTTACTCGGGGCAGTATCAACCATACTCATTGCAATCTTTACCTCATCGTGCCTGATGCACATCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCAACCGGCAAAGTGATAACCGTGTACAGAGTCAAAAAgc                                                                                                      >  2:611491/1‑149 (MQ=255)
                                               aaTTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCATGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgcg                                                                                                    <  1:684098/150‑1 (MQ=255)
                                               aaTTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGgcg                                                                                                    <  1:1175740/150‑1 (MQ=255)
                                                    tACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcgg                                                                                               <  2:1430434/150‑1 (MQ=255)
                                                       tccgggCAGCTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATTCACTTCGCTAATCAGGCTTACCGTAGTCCGGATCAGGCGTTCACGCAGCATCAGGCAAAGTGTTAACCGTGTACTGAGTCAAAAAGGCGCtcggtcg                                                                                            <  2:1554865/147‑1 (MQ=255)
                                                       tCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCtcggtcg                                                                                            >  2:722977/1‑150 (MQ=255)
                                                         ggggCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCTTACACGCCCCATCCGTCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCGcggtcggt                                                                                          >  1:1549894/1‑150 (MQ=255)
                                                           ggCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTcc                                                                                        <  1:702506/150‑1 (MQ=255)
                                                                tttCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATACGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCACg                                                                                   >  1:457414/1‑150 (MQ=255)
                                                                  tCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCACCTCGcc                                                                                 <  1:29136/150‑1 (MQ=255)
                                                                      ccATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCctc                                                                             <  2:1622418/150‑1 (MQ=255)
                                                                      ccATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCctc                                                                             >  1:956856/1‑150 (MQ=255)
                                                                        aTACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTg                                                                           >  2:1421592/1‑150 (MQ=255)
                                                                         tACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTccccagagg                                                                          <  2:1375107/150‑6 (MQ=255)
                                                                          aCTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTggg                                                                         >  1:1356801/1‑150 (MQ=255)
                                                                                tGCACTCTTTTCCTCATCGTGCCTGATGCACTTCGCTCATCACGCTAACCGTAGGCCGGATCAGGCGATCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                   <  2:698723/150‑1 (MQ=255)
                                                                                tGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                   <  1:1628789/150‑1 (MQ=39)
                                                                                 ggattCTTTTCCTCATCGTGCCCGTTGCACCTCGCTTATCACGCCTACCGTAGGCCGGAGCAGGCGTGCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGGCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAggg                                                                  <  1:658442/146‑1 (MQ=255)
                                                                                 gCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                  <  1:1098380/150‑1 (MQ=255)
                                                                                    aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCAGAGGGGCGAggggac                                                               <  1:1219623/150‑4 (MQ=39)
                                                                                    aTCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                               <  2:1335307/150‑1 (MQ=255)
                                                                                     tCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCTCCCCTCTGGGGAGAGGGTTAg                                                              >  2:1395423/1‑150 (MQ=255)
                                                                                       ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                        <  2:184204/138‑1 (MQ=255)
                                                                                       ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTgggg                                                                        >  1:184204/1‑138 (MQ=255)
                                                                                       ttttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                            >  1:1445780/1‑150 (MQ=255)
                                                                                         ttCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                          <  2:1683471/150‑1 (MQ=255)
                                                                                          tCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGa                                                         >  1:673759/1‑150 (MQ=255)
                                                                                            cTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                       <  1:625067/150‑1 (MQ=255)
                                                                                             tCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                      <  2:1263621/150‑1 (MQ=255)
                                                                                              cATCGTGCCTGATGCACTTCGCTACTCCGGCTTACCGTCGGCCGGATCCGGCGGGCACGCCGCAGCCGGCAAAGTGGTCACCGTGTACCGAGGCAACACGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgggg                                                     <  1:920179/150‑1 (MQ=25)
                                                                                               aTCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTACCCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGCGGGTTAGGGTGAGGGGa                                                    >  2:1054542/1‑150 (MQ=255)
                                                                                                tCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                   >  2:745105/1‑150 (MQ=255)
                                                                                                 cGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                  >  1:1382884/1‑150 (MQ=255)
                                                                                                   tGCCTGATGCACTTCGCTATTGAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAAGGGGCGCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAc                                                <  1:1314315/150‑1 (MQ=38)
                                                                                                   ggcgtGATGCACTTCGCTAATCAGGCTTACCGTAGGCTGGATCAGGCGTTCACGCCGAATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGGCCCCTCGCCCCTCTGGGGAGAGGGTTAGTGTGTGGGGAAAAc                                                <  2:1549894/146‑1 (MQ=39)
                                                                                                    gCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTTTACAGAGTCAAAAAGGCGCTCGGTCGGGCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAcc                                               <  1:826687/150‑1 (MQ=255)
                                                                                                     ccTGATTCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGGGTTAACCGGGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCGCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCg                                              <  1:1606675/150‑1 (MQ=255)
                                                                                                     ccTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGTCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCg                                              <  2:973410/150‑1 (MQ=255)
                                                                                                        gATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAACGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCgtgt                                           <  1:722977/150‑1 (MQ=255)
                                                                                                                        cAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCgt                           >  1:1380723/1‑150 (MQ=255)
                                                                                                                            cTTACCGTAGGCCGGATCAGGCGTTCACCCCGCATCCGGAAATGTGTTAACCGTGTACAGAGTCAAAACGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGTGTGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGttt                       <  2:1445780/150‑1 (MQ=39)
                                                                                                                              tACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAACCCGTGTCAGCAAGATCAACCGTGTTTAt                     >  2:897344/1‑150 (MQ=255)
                                                                                                                                 cGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttct                  <  2:1241115/150‑1 (MQ=255)
                                                                                                                                   tAGGCCGGAGCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTAttcttc                <  1:25798/150‑1 (MQ=255)
                                                                                                                                    ttgCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg               >  2:893265/3‑150 (MQ=38)
                                                                                                                                    tggCCGGAGCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg               <  2:1356801/149‑1 (MQ=255)
                                                                                                                                    aGGCGGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTAAAGATTCAAAATGGGGCGCGGGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGTGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg               <  2:1341880/150‑1 (MQ=38)
                                                                                                                                    aGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg               <  1:632373/150‑1 (MQ=255)
                                                                                                                                    aGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCg               >  2:922094/1‑150 (MQ=255)
                                                                                                                                     ggCCGGAGCAGGGGTGCACGCCGCATCGGGAAAAGTGTTAACCGTGTACAGAGTCAAAACGGCGCTGGGGCGGGCCCCTCGCCCCTCTGGGGTTAGGGTTAGGGGGCGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGc              <  2:1253564/150‑1 (MQ=255)
                                                                                                                                      gCCGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATAACAACCGTGTTTATTCTTCGCa             >  2:319320/1‑150 (MQ=38)
                                                                                                                                       ccGGATGCGGCGTGAACGCCTGATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTTGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaa            >  1:747257/1‑150 (MQ=38)
                                                                                                                                       ccGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGCGTGTCAGCAATATCACCCGTGTTTATTCTTCGCag            >  1:117320/1‑149 (MQ=255)
                                                                                                                                        cGGATCAGGCGTTCACGCCGCATCCGGAAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCaaa           <  2:388198/150‑1 (MQ=255)
                                                                                                                                            tCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaaca       <  2:956856/150‑1 (MQ=255)
                                                                                                                                             cAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaa      >  1:1294678/1‑150 (MQ=255)
                                                                                                                                             cAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAaacaa      >  2:740336/1‑150 (MQ=255)
                                                                                                                                               ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCATCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcg    >  2:251087/1‑150 (MQ=255)
                                                                                                                                               ggCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCATCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAacaacacag    >  2:282038/1‑147 (MQ=255)
                                                                                                                                                 cGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGATGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAAcgcg  <  1:113794/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TTCAGGGCATGGAATTTGCCGTAGTAGAAGTTCAAATTACGAACCTGAATTTTACTCGGGGCAGTTTCAACCATACTCATTGCAATCTTTTCCTCATCGTGCCTGATGCACTTCGCTAATCAGGCTTACCGTAGGCCGGATCAGGCGTTCACGCCGCATCCGGCAAAGTGTTAACCGTGTACAGAGTCAAAAAGGCGCTCGGTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAACCGTGTCAGCAATATCAACCGTGTTTATTCTTCGCAAAAACAACGCG  >  NC_000913/3908287‑3908581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑